Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_335

Experiment: sodium fluoride 12.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_334 and Pf6N2E2_335 are separated by 100 nucleotidesPf6N2E2_335 and Pf6N2E2_336 overlap by 4 nucleotidesPf6N2E2_336 and Pf6N2E2_337 are separated by 173 nucleotides Pf6N2E2_334 - Transcriptional regulator, at 401,717 to 402,670 _334 Pf6N2E2_335 - Aldo-keto reductase, at 402,771 to 403,634 _335 Pf6N2E2_336 - hypothetical protein, at 403,631 to 404,413 _336 Pf6N2E2_337 - hypothetical protein, at 404,587 to 404,859 _337 Position (kb) 402 403 404Strain fitness (log2 ratio) -1 0 1 2at 401.838 kb on + strand, within Pf6N2E2_334at 401.841 kb on + strand, within Pf6N2E2_334at 401.910 kb on + strand, within Pf6N2E2_334at 401.937 kb on + strand, within Pf6N2E2_334at 401.937 kb on + strand, within Pf6N2E2_334at 401.937 kb on + strand, within Pf6N2E2_334at 401.938 kb on - strand, within Pf6N2E2_334at 401.957 kb on + strand, within Pf6N2E2_334at 401.958 kb on - strand, within Pf6N2E2_334at 402.051 kb on + strand, within Pf6N2E2_334at 402.051 kb on + strand, within Pf6N2E2_334at 402.150 kb on + strand, within Pf6N2E2_334at 402.150 kb on + strand, within Pf6N2E2_334at 402.151 kb on - strand, within Pf6N2E2_334at 402.211 kb on + strand, within Pf6N2E2_334at 402.212 kb on - strand, within Pf6N2E2_334at 402.337 kb on + strand, within Pf6N2E2_334at 402.686 kb on - strandat 402.733 kb on + strandat 402.733 kb on + strandat 402.733 kb on + strandat 402.733 kb on + strandat 402.733 kb on + strandat 402.734 kb on - strandat 402.734 kb on - strandat 402.734 kb on - strandat 402.734 kb on - strandat 402.734 kb on - strandat 402.734 kb on - strandat 402.957 kb on + strand, within Pf6N2E2_335at 403.006 kb on - strand, within Pf6N2E2_335at 403.332 kb on + strand, within Pf6N2E2_335at 403.332 kb on + strand, within Pf6N2E2_335at 403.395 kb on + strand, within Pf6N2E2_335at 403.395 kb on + strand, within Pf6N2E2_335at 403.396 kb on - strand, within Pf6N2E2_335at 403.396 kb on - strand, within Pf6N2E2_335at 403.396 kb on - strand, within Pf6N2E2_335at 403.633 kb on - strandat 403.865 kb on + strand, within Pf6N2E2_336at 403.865 kb on + strand, within Pf6N2E2_336at 403.865 kb on + strand, within Pf6N2E2_336at 403.865 kb on + strand, within Pf6N2E2_336at 403.866 kb on - strand, within Pf6N2E2_336at 403.866 kb on - strandat 403.866 kb on - strand, within Pf6N2E2_336at 403.938 kb on + strand, within Pf6N2E2_336at 403.938 kb on + strand, within Pf6N2E2_336at 403.938 kb on + strand, within Pf6N2E2_336at 404.039 kb on - strand, within Pf6N2E2_336at 404.039 kb on - strand, within Pf6N2E2_336at 404.039 kb on - strand, within Pf6N2E2_336at 404.116 kb on - strand, within Pf6N2E2_336at 404.467 kb on + strandat 404.467 kb on + strandat 404.536 kb on + strandat 404.536 kb on + strandat 404.536 kb on + strandat 404.537 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM
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401,838 + Pf6N2E2_334 0.13 -0.3
401,841 + Pf6N2E2_334 0.13 +0.3
401,910 + Pf6N2E2_334 0.20 -0.2
401,937 + Pf6N2E2_334 0.23 -0.1
401,937 + Pf6N2E2_334 0.23 -0.0
401,937 + Pf6N2E2_334 0.23 +1.0
401,938 - Pf6N2E2_334 0.23 -0.0
401,957 + Pf6N2E2_334 0.25 +0.5
401,958 - Pf6N2E2_334 0.25 +0.9
402,051 + Pf6N2E2_334 0.35 +0.1
402,051 + Pf6N2E2_334 0.35 -0.2
402,150 + Pf6N2E2_334 0.45 +0.8
402,150 + Pf6N2E2_334 0.45 -0.0
402,151 - Pf6N2E2_334 0.45 -0.3
402,211 + Pf6N2E2_334 0.52 +0.1
402,212 - Pf6N2E2_334 0.52 -0.5
402,337 + Pf6N2E2_334 0.65 -0.1
402,686 - -1.5
402,733 + +0.5
402,733 + +0.1
402,733 + -0.0
402,733 + +0.5
402,733 + -0.7
402,734 - -0.6
402,734 - -0.6
402,734 - -1.2
402,734 - +0.9
402,734 - -0.3
402,734 - -0.4
402,957 + Pf6N2E2_335 0.22 -0.5
403,006 - Pf6N2E2_335 0.27 -1.0
403,332 + Pf6N2E2_335 0.65 +0.2
403,332 + Pf6N2E2_335 0.65 +0.1
403,395 + Pf6N2E2_335 0.72 -0.2
403,395 + Pf6N2E2_335 0.72 -0.8
403,396 - Pf6N2E2_335 0.72 +0.0
403,396 - Pf6N2E2_335 0.72 +0.3
403,396 - Pf6N2E2_335 0.72 -0.6
403,633 - +0.4
403,865 + Pf6N2E2_336 0.30 +0.1
403,865 + Pf6N2E2_336 0.30 +1.2
403,865 + Pf6N2E2_336 0.30 +0.6
403,865 + Pf6N2E2_336 0.30 +0.3
403,866 - Pf6N2E2_336 0.30 -0.3
403,866 - +2.4
403,866 - Pf6N2E2_336 0.30 -0.3
403,938 + Pf6N2E2_336 0.39 +1.0
403,938 + Pf6N2E2_336 0.39 -0.4
403,938 + Pf6N2E2_336 0.39 -0.6
404,039 - Pf6N2E2_336 0.52 +0.3
404,039 - Pf6N2E2_336 0.52 +0.4
404,039 - Pf6N2E2_336 0.52 +0.5
404,116 - Pf6N2E2_336 0.62 +1.1
404,467 + -0.2
404,467 + +1.3
404,536 + -0.1
404,536 + +0.2
404,536 + +0.5
404,537 - +0.4

Or see this region's nucleotide sequence