Experiment: sodium fluoride 12.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2928 and Pf6N2E2_2929 overlap by 4 nucleotides Pf6N2E2_2929 and Pf6N2E2_2930 overlap by 4 nucleotides Pf6N2E2_2930 and Pf6N2E2_2931 are separated by 84 nucleotides
Pf6N2E2_2928 - Uncharacterized protein conserved in bacteria, NMA0228-like, at 3,420,506 to 3,421,375
_2928
Pf6N2E2_2929 - Glutamate synthase [NADPH] large chain (EC 1.4.1.13), at 3,421,372 to 3,422,130
_2929
Pf6N2E2_2930 - INTEGRAL MEMBRANE PROTEIN (Rhomboid family), at 3,422,127 to 3,422,606
_2930
Pf6N2E2_2931 - Transcriptional regulator, LysR family, at 3,422,691 to 3,423,647
_2931
Position (kb)
3422
3423 Strain fitness (log2 ratio)
-1
0
1 at 3421.194 kb on + strand, within Pf6N2E2_2928 at 3421.238 kb on + strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.239 kb on - strand, within Pf6N2E2_2928 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.585 kb on + strand, within Pf6N2E2_2929 at 3421.586 kb on - strand, within Pf6N2E2_2929 at 3421.684 kb on + strand, within Pf6N2E2_2929 at 3421.685 kb on - strand, within Pf6N2E2_2929 at 3421.685 kb on - strand, within Pf6N2E2_2929 at 3422.101 kb on + strand at 3422.101 kb on + strand at 3422.101 kb on + strand at 3422.101 kb on + strand at 3422.102 kb on - strand at 3422.102 kb on - strand at 3422.108 kb on + strand at 3422.108 kb on + strand at 3422.108 kb on + strand at 3422.109 kb on - strand at 3422.121 kb on - strand at 3422.121 kb on - strand at 3422.513 kb on + strand, within Pf6N2E2_2930 at 3422.513 kb on + strand, within Pf6N2E2_2930 at 3422.513 kb on + strand, within Pf6N2E2_2930 at 3422.513 kb on + strand, within Pf6N2E2_2930 at 3422.513 kb on + strand, within Pf6N2E2_2930 at 3422.536 kb on - strand, within Pf6N2E2_2930 at 3422.620 kb on + strand at 3422.621 kb on - strand at 3422.621 kb on - strand at 3422.621 kb on - strand at 3422.621 kb on - strand at 3422.693 kb on - strand at 3422.747 kb on - strand at 3422.747 kb on - strand at 3422.788 kb on + strand, within Pf6N2E2_2931 at 3422.803 kb on + strand, within Pf6N2E2_2931 at 3422.803 kb on + strand, within Pf6N2E2_2931 at 3422.803 kb on + strand, within Pf6N2E2_2931 at 3422.804 kb on - strand, within Pf6N2E2_2931 at 3422.804 kb on - strand, within Pf6N2E2_2931 at 3422.804 kb on - strand, within Pf6N2E2_2931 at 3422.804 kb on - strand, within Pf6N2E2_2931 at 3422.804 kb on - strand, within Pf6N2E2_2931 at 3422.938 kb on + strand, within Pf6N2E2_2931 at 3422.938 kb on + strand, within Pf6N2E2_2931 at 3422.939 kb on - strand, within Pf6N2E2_2931 at 3422.939 kb on - strand, within Pf6N2E2_2931 at 3422.946 kb on - strand, within Pf6N2E2_2931 at 3422.998 kb on + strand, within Pf6N2E2_2931 at 3422.999 kb on - strand, within Pf6N2E2_2931 at 3422.999 kb on - strand, within Pf6N2E2_2931 at 3422.999 kb on - strand, within Pf6N2E2_2931 at 3423.022 kb on + strand, within Pf6N2E2_2931 at 3423.022 kb on + strand, within Pf6N2E2_2931 at 3423.023 kb on - strand, within Pf6N2E2_2931 at 3423.023 kb on - strand, within Pf6N2E2_2931 at 3423.029 kb on + strand, within Pf6N2E2_2931 at 3423.082 kb on + strand, within Pf6N2E2_2931 at 3423.253 kb on + strand, within Pf6N2E2_2931 at 3423.254 kb on - strand, within Pf6N2E2_2931 at 3423.258 kb on - strand, within Pf6N2E2_2931 at 3423.258 kb on - strand, within Pf6N2E2_2931 at 3423.258 kb on - strand, within Pf6N2E2_2931 at 3423.258 kb on - strand, within Pf6N2E2_2931 at 3423.338 kb on + strand, within Pf6N2E2_2931
Per-strain Table
Position Strand Gene LocusTag Fraction sodium fluoride 12.5 mM remove 3,421,194 + Pf6N2E2_2928 0.79 -0.9 3,421,238 + Pf6N2E2_2928 0.84 +0.6 3,421,239 - Pf6N2E2_2928 0.84 -1.2 3,421,239 - Pf6N2E2_2928 0.84 +0.7 3,421,239 - Pf6N2E2_2928 0.84 -0.1 3,421,239 - Pf6N2E2_2928 0.84 -1.0 3,421,239 - Pf6N2E2_2928 0.84 -0.3 3,421,239 - Pf6N2E2_2928 0.84 -0.1 3,421,585 + Pf6N2E2_2929 0.28 -0.6 3,421,585 + Pf6N2E2_2929 0.28 -0.4 3,421,585 + Pf6N2E2_2929 0.28 +0.9 3,421,585 + Pf6N2E2_2929 0.28 +1.1 3,421,586 - Pf6N2E2_2929 0.28 +0.4 3,421,684 + Pf6N2E2_2929 0.41 -0.7 3,421,685 - Pf6N2E2_2929 0.41 +0.4 3,421,685 - Pf6N2E2_2929 0.41 -0.1 3,422,101 + -0.7 3,422,101 + +0.1 3,422,101 + +1.3 3,422,101 + -0.7 3,422,102 - +0.1 3,422,102 - -0.6 3,422,108 + +1.2 3,422,108 + +0.2 3,422,108 + +0.3 3,422,109 - +0.0 3,422,121 - -0.1 3,422,121 - +0.7 3,422,513 + Pf6N2E2_2930 0.80 +0.4 3,422,513 + Pf6N2E2_2930 0.80 -0.3 3,422,513 + Pf6N2E2_2930 0.80 -0.7 3,422,513 + Pf6N2E2_2930 0.80 -0.6 3,422,513 + Pf6N2E2_2930 0.80 -0.5 3,422,536 - Pf6N2E2_2930 0.85 -0.6 3,422,620 + +0.4 3,422,621 - -0.5 3,422,621 - +0.9 3,422,621 - +0.6 3,422,621 - +0.7 3,422,693 - +0.2 3,422,747 - +0.2 3,422,747 - -0.1 3,422,788 + Pf6N2E2_2931 0.10 +0.8 3,422,803 + Pf6N2E2_2931 0.12 -1.1 3,422,803 + Pf6N2E2_2931 0.12 -0.4 3,422,803 + Pf6N2E2_2931 0.12 +0.5 3,422,804 - Pf6N2E2_2931 0.12 +1.1 3,422,804 - Pf6N2E2_2931 0.12 +0.1 3,422,804 - Pf6N2E2_2931 0.12 +0.4 3,422,804 - Pf6N2E2_2931 0.12 +1.3 3,422,804 - Pf6N2E2_2931 0.12 +0.1 3,422,938 + Pf6N2E2_2931 0.26 +0.2 3,422,938 + Pf6N2E2_2931 0.26 +0.6 3,422,939 - Pf6N2E2_2931 0.26 +1.6 3,422,939 - Pf6N2E2_2931 0.26 +0.8 3,422,946 - Pf6N2E2_2931 0.27 +0.0 3,422,998 + Pf6N2E2_2931 0.32 -1.3 3,422,999 - Pf6N2E2_2931 0.32 +0.3 3,422,999 - Pf6N2E2_2931 0.32 +0.5 3,422,999 - Pf6N2E2_2931 0.32 -0.3 3,423,022 + Pf6N2E2_2931 0.35 -0.8 3,423,022 + Pf6N2E2_2931 0.35 -0.3 3,423,023 - Pf6N2E2_2931 0.35 -0.0 3,423,023 - Pf6N2E2_2931 0.35 +0.6 3,423,029 + Pf6N2E2_2931 0.35 +0.1 3,423,082 + Pf6N2E2_2931 0.41 -0.8 3,423,253 + Pf6N2E2_2931 0.59 -0.1 3,423,254 - Pf6N2E2_2931 0.59 +0.7 3,423,258 - Pf6N2E2_2931 0.59 +0.5 3,423,258 - Pf6N2E2_2931 0.59 +0.4 3,423,258 - Pf6N2E2_2931 0.59 -0.5 3,423,258 - Pf6N2E2_2931 0.59 +0.4 3,423,338 + Pf6N2E2_2931 0.68 -0.3
Or see this region's nucleotide sequence