Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS00375 and MMJJ_RS00380 are separated by 63 nucleotides MMJJ_RS00380 and MMJJ_RS00385 overlap by 4 nucleotides MMJJ_RS00385 and MMJJ_RS00390 are separated by 84 nucleotides MMJJ_RS00390 and MMJJ_RS00395 are separated by 18 nucleotides
MMJJ_RS00375: MMJJ_RS00375 - diaminopimelate epimerase, at 72,112 to 72,945
_RS00375
MMJJ_RS00380: MMJJ_RS00380 - PRC-barrel domain-containing protein, at 73,009 to 73,257
_RS00380
MMJJ_RS00385: MMJJ_RS00385 - TIGR00454 family protein, at 73,254 to 73,862
_RS00385
MMJJ_RS00390: MMJJ_RS00390 - proteasome assembly chaperone family protein, at 73,947 to 74,654
_RS00390
MMJJ_RS00395: MMJJ_RS00395 - DUF473 domain-containing protein, at 74,673 to 75,050
_RS00395
Position (kb) 73
74 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 72.898 kb on - strand at 72.908 kb on - strand at 72.909 kb on + strand at 72.968 kb on - strand at 72.993 kb on - strand at 73.010 kb on - strand at 73.092 kb on + strand at 73.151 kb on + strand at 73.377 kb on - strand, within MMJJ_RS00385 at 73.378 kb on + strand, within MMJJ_RS00385 at 73.395 kb on - strand, within MMJJ_RS00385 at 73.433 kb on + strand at 73.515 kb on + strand, within MMJJ_RS00385 at 73.586 kb on - strand, within MMJJ_RS00385 at 73.587 kb on + strand, within MMJJ_RS00385 at 73.587 kb on + strand, within MMJJ_RS00385 at 73.596 kb on - strand, within MMJJ_RS00385 at 73.596 kb on - strand, within MMJJ_RS00385 at 73.597 kb on + strand, within MMJJ_RS00385 at 73.609 kb on - strand, within MMJJ_RS00385 at 73.609 kb on - strand, within MMJJ_RS00385 at 73.626 kb on + strand, within MMJJ_RS00385 at 73.632 kb on - strand, within MMJJ_RS00385 at 73.632 kb on - strand, within MMJJ_RS00385 at 73.632 kb on - strand, within MMJJ_RS00385 at 73.632 kb on - strand, within MMJJ_RS00385 at 73.632 kb on - strand, within MMJJ_RS00385 at 73.633 kb on + strand, within MMJJ_RS00385 at 73.633 kb on + strand, within MMJJ_RS00385 at 73.633 kb on + strand, within MMJJ_RS00385 at 73.635 kb on + strand, within MMJJ_RS00385 at 73.635 kb on + strand, within MMJJ_RS00385 at 73.635 kb on + strand, within MMJJ_RS00385 at 73.638 kb on - strand, within MMJJ_RS00385 at 73.638 kb on - strand, within MMJJ_RS00385 at 73.639 kb on + strand, within MMJJ_RS00385 at 73.652 kb on + strand, within MMJJ_RS00385 at 73.684 kb on - strand, within MMJJ_RS00385 at 73.685 kb on + strand, within MMJJ_RS00385 at 73.713 kb on - strand, within MMJJ_RS00385 at 73.723 kb on - strand, within MMJJ_RS00385 at 73.725 kb on - strand, within MMJJ_RS00385 at 73.726 kb on + strand, within MMJJ_RS00385 at 73.765 kb on - strand, within MMJJ_RS00385 at 73.886 kb on - strand at 73.887 kb on + strand at 73.887 kb on + strand at 73.893 kb on - strand at 73.893 kb on - strand at 73.893 kb on - strand at 73.893 kb on - strand at 73.893 kb on - strand at 73.893 kb on - strand at 73.893 kb on - strand at 73.893 kb on - strand at 73.893 kb on - strand at 73.894 kb on + strand at 73.894 kb on + strand at 73.895 kb on - strand at 73.897 kb on - strand at 73.898 kb on + strand at 73.947 kb on + strand at 73.953 kb on - strand at 73.953 kb on - strand at 73.954 kb on + strand at 73.980 kb on + strand at 74.070 kb on - strand, within MMJJ_RS00390 at 74.079 kb on - strand, within MMJJ_RS00390 at 74.151 kb on - strand, within MMJJ_RS00390 at 74.168 kb on + strand, within MMJJ_RS00390 at 74.171 kb on + strand, within MMJJ_RS00390 at 74.173 kb on - strand, within MMJJ_RS00390 at 74.188 kb on - strand, within MMJJ_RS00390 at 74.188 kb on - strand, within MMJJ_RS00390 at 74.188 kb on - strand, within MMJJ_RS00390 at 74.188 kb on - strand, within MMJJ_RS00390 at 74.207 kb on + strand, within MMJJ_RS00390 at 74.207 kb on + strand, within MMJJ_RS00390 at 74.209 kb on - strand, within MMJJ_RS00390 at 74.209 kb on - strand, within MMJJ_RS00390 at 74.209 kb on - strand, within MMJJ_RS00390 at 74.209 kb on - strand, within MMJJ_RS00390 at 74.210 kb on + strand, within MMJJ_RS00390 at 74.210 kb on + strand, within MMJJ_RS00390 at 74.216 kb on - strand, within MMJJ_RS00390 at 74.216 kb on - strand, within MMJJ_RS00390 at 74.216 kb on - strand, within MMJJ_RS00390 at 74.216 kb on - strand, within MMJJ_RS00390 at 74.217 kb on + strand, within MMJJ_RS00390 at 74.217 kb on + strand, within MMJJ_RS00390 at 74.217 kb on + strand, within MMJJ_RS00390 at 74.217 kb on + strand, within MMJJ_RS00390 at 74.217 kb on + strand, within MMJJ_RS00390 at 74.225 kb on - strand, within MMJJ_RS00390 at 74.226 kb on + strand, within MMJJ_RS00390 at 74.252 kb on + strand at 74.270 kb on + strand, within MMJJ_RS00390 at 74.385 kb on + strand, within MMJJ_RS00390 at 74.385 kb on + strand, within MMJJ_RS00390 at 74.408 kb on - strand, within MMJJ_RS00390 at 74.409 kb on + strand, within MMJJ_RS00390 at 74.421 kb on + strand, within MMJJ_RS00390 at 74.470 kb on - strand, within MMJJ_RS00390 at 74.496 kb on + strand, within MMJJ_RS00390 at 74.501 kb on + strand, within MMJJ_RS00390 at 74.512 kb on - strand, within MMJJ_RS00390 at 74.642 kb on - strand at 74.642 kb on - strand at 74.642 kb on - strand at 74.643 kb on + strand at 74.646 kb on - strand at 74.646 kb on - strand at 74.646 kb on - strand at 74.646 kb on - strand at 74.646 kb on - strand at 74.646 kb on - strand at 74.646 kb on - strand at 74.646 kb on - strand at 74.646 kb on - strand at 74.646 kb on - strand at 74.646 kb on - strand at 74.646 kb on - strand at 74.647 kb on + strand at 74.647 kb on + strand at 74.647 kb on + strand at 74.647 kb on + strand at 74.647 kb on + strand at 74.647 kb on + strand at 74.647 kb on + strand at 74.647 kb on + strand at 74.647 kb on + strand at 74.647 kb on + strand at 74.647 kb on + strand at 74.648 kb on - strand at 74.649 kb on + strand at 74.653 kb on + strand at 74.653 kb on + strand at 74.653 kb on + strand at 74.765 kb on - strand, within MMJJ_RS00395
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE remove 72,898 - -3.2 72,908 - -3.7 72,909 + -1.4 72,968 - -1.9 72,993 - -1.1 73,010 - +0.4 73,092 + -0.4 73,151 + -0.4 73,377 - MMJJ_RS00385 0.20 -3.7 73,378 + MMJJ_RS00385 0.20 -0.4 73,395 - MMJJ_RS00385 0.23 -3.7 73,433 + -1.4 73,515 + MMJJ_RS00385 0.43 -0.4 73,586 - MMJJ_RS00385 0.55 -3.3 73,587 + MMJJ_RS00385 0.55 -3.3 73,587 + MMJJ_RS00385 0.55 -2.7 73,596 - MMJJ_RS00385 0.56 -2.1 73,596 - MMJJ_RS00385 0.56 -3.4 73,597 + MMJJ_RS00385 0.56 -2.4 73,609 - MMJJ_RS00385 0.58 -3.6 73,609 - MMJJ_RS00385 0.58 -1.8 73,626 + MMJJ_RS00385 0.61 -2.7 73,632 - MMJJ_RS00385 0.62 -0.9 73,632 - MMJJ_RS00385 0.62 -2.4 73,632 - MMJJ_RS00385 0.62 -2.7 73,632 - MMJJ_RS00385 0.62 -0.5 73,632 - MMJJ_RS00385 0.62 -0.6 73,633 + MMJJ_RS00385 0.62 -2.2 73,633 + MMJJ_RS00385 0.62 -2.6 73,633 + MMJJ_RS00385 0.62 -0.4 73,635 + MMJJ_RS00385 0.63 -2.4 73,635 + MMJJ_RS00385 0.63 -1.4 73,635 + MMJJ_RS00385 0.63 -3.4 73,638 - MMJJ_RS00385 0.63 -3.2 73,638 - MMJJ_RS00385 0.63 -2.3 73,639 + MMJJ_RS00385 0.63 -1.6 73,652 + MMJJ_RS00385 0.65 -0.4 73,684 - MMJJ_RS00385 0.71 -2.7 73,685 + MMJJ_RS00385 0.71 -3.6 73,713 - MMJJ_RS00385 0.75 -2.4 73,723 - MMJJ_RS00385 0.77 -3.4 73,725 - MMJJ_RS00385 0.77 -1.4 73,726 + MMJJ_RS00385 0.78 -3.2 73,765 - MMJJ_RS00385 0.84 -2.5 73,886 - -1.2 73,887 + -1.2 73,887 + -0.4 73,893 - -1.4 73,893 - -0.4 73,893 - -0.0 73,893 - -0.6 73,893 - -0.8 73,893 - +0.0 73,893 - -0.6 73,893 - -0.0 73,893 - -0.2 73,894 + -2.4 73,894 + -3.7 73,895 - +0.5 73,897 - +0.8 73,898 + -1.1 73,947 + -0.0 73,953 - +2.0 73,953 - +1.2 73,954 + +0.9 73,980 + +0.1 74,070 - MMJJ_RS00390 0.17 +0.4 74,079 - MMJJ_RS00390 0.19 +0.6 74,151 - MMJJ_RS00390 0.29 +1.7 74,168 + MMJJ_RS00390 0.31 -0.2 74,171 + MMJJ_RS00390 0.32 -0.8 74,173 - MMJJ_RS00390 0.32 -0.4 74,188 - MMJJ_RS00390 0.34 +0.6 74,188 - MMJJ_RS00390 0.34 +0.0 74,188 - MMJJ_RS00390 0.34 +0.9 74,188 - MMJJ_RS00390 0.34 +0.0 74,207 + MMJJ_RS00390 0.37 +0.1 74,207 + MMJJ_RS00390 0.37 -0.2 74,209 - MMJJ_RS00390 0.37 -0.5 74,209 - MMJJ_RS00390 0.37 -0.5 74,209 - MMJJ_RS00390 0.37 +0.7 74,209 - MMJJ_RS00390 0.37 +1.0 74,210 + MMJJ_RS00390 0.37 -0.7 74,210 + MMJJ_RS00390 0.37 -0.3 74,216 - MMJJ_RS00390 0.38 -0.7 74,216 - MMJJ_RS00390 0.38 +0.3 74,216 - MMJJ_RS00390 0.38 +0.6 74,216 - MMJJ_RS00390 0.38 +0.2 74,217 + MMJJ_RS00390 0.38 +1.7 74,217 + MMJJ_RS00390 0.38 -0.7 74,217 + MMJJ_RS00390 0.38 -0.4 74,217 + MMJJ_RS00390 0.38 +0.0 74,217 + MMJJ_RS00390 0.38 +0.6 74,225 - MMJJ_RS00390 0.39 -0.2 74,226 + MMJJ_RS00390 0.39 -0.7 74,252 + +0.0 74,270 + MMJJ_RS00390 0.46 +0.4 74,385 + MMJJ_RS00390 0.62 -0.6 74,385 + MMJJ_RS00390 0.62 +2.8 74,408 - MMJJ_RS00390 0.65 +0.3 74,409 + MMJJ_RS00390 0.65 +1.5 74,421 + MMJJ_RS00390 0.67 -1.0 74,470 - MMJJ_RS00390 0.74 -0.5 74,496 + MMJJ_RS00390 0.78 -0.8 74,501 + MMJJ_RS00390 0.78 +1.9 74,512 - MMJJ_RS00390 0.80 -1.1 74,642 - -1.1 74,642 - +0.2 74,642 - +0.2 74,643 + -1.1 74,646 - -0.4 74,646 - -0.5 74,646 - +0.1 74,646 - -0.4 74,646 - +0.2 74,646 - -0.5 74,646 - +0.2 74,646 - -0.1 74,646 - +0.3 74,646 - +0.3 74,646 - -0.3 74,646 - -0.5 74,647 + +0.4 74,647 + +0.5 74,647 + -0.1 74,647 + -0.4 74,647 + +3.4 74,647 + -1.4 74,647 + +0.0 74,647 + -0.7 74,647 + +0.1 74,647 + +0.1 74,647 + +2.5 74,648 - +1.0 74,649 + +2.2 74,653 + -0.4 74,653 + +0.7 74,653 + +2.6 74,765 - MMJJ_RS00395 0.24 +0.1
Or see this region's nucleotide sequence