Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS00340

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS00330 and MMJJ_RS00335 are separated by 43 nucleotidesMMJJ_RS00335 and MMJJ_RS00340 are separated by 10 nucleotidesMMJJ_RS00340 and MMJJ_RS00345 are separated by 8 nucleotidesMMJJ_RS00345 and MMJJ_RS00350 are separated by 8 nucleotides MMJJ_RS00330: MMJJ_RS00330 - chemotaxis response regulator protein-glutamate methylesterase, at 64,115 to 65,212 _RS00330 MMJJ_RS00335: MMJJ_RS00335 - chemotaxis protein CheW, at 65,256 to 65,699 _RS00335 MMJJ_RS00340: MMJJ_RS00340 - tRNA (pseudouridine(54)-N(1))-methyltransferase TrmY, at 65,710 to 66,306 _RS00340 MMJJ_RS00345: MMJJ_RS00345 - 4-hydroxy-tetrahydrodipicolinate reductase, at 66,315 to 67,127 _RS00345 MMJJ_RS00350: MMJJ_RS00350 - phage infection protein, at 67,136 to 67,786 _RS00350 Position (kb) 65 66 67Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3 4at 64.747 kb on - strand, within MMJJ_RS00330at 64.777 kb on - strand, within MMJJ_RS00330at 64.808 kb on - strand, within MMJJ_RS00330at 64.808 kb on - strand, within MMJJ_RS00330at 64.809 kb on + strand, within MMJJ_RS00330at 64.931 kb on - strand, within MMJJ_RS00330at 64.932 kb on + strand, within MMJJ_RS00330at 64.936 kb on - strand, within MMJJ_RS00330at 65.012 kb on + strand, within MMJJ_RS00330at 65.054 kb on + strand, within MMJJ_RS00330at 65.058 kb on + strand, within MMJJ_RS00330at 65.186 kb on - strandat 65.231 kb on - strandat 65.305 kb on + strand, within MMJJ_RS00335at 65.309 kb on - strand, within MMJJ_RS00335at 65.338 kb on - strand, within MMJJ_RS00335at 65.338 kb on - strand, within MMJJ_RS00335at 65.404 kb on + strand, within MMJJ_RS00335at 65.427 kb on - strand, within MMJJ_RS00335at 65.463 kb on - strand, within MMJJ_RS00335at 65.463 kb on - strand, within MMJJ_RS00335at 65.464 kb on + strand, within MMJJ_RS00335at 65.542 kb on + strand, within MMJJ_RS00335at 65.562 kb on + strand, within MMJJ_RS00335at 65.637 kb on - strand, within MMJJ_RS00335at 65.680 kb on + strandat 65.721 kb on - strandat 65.721 kb on - strandat 65.722 kb on + strandat 65.726 kb on + strandat 65.726 kb on + strandat 65.726 kb on + strandat 65.734 kb on + strandat 65.746 kb on - strandat 65.746 kb on - strandat 65.747 kb on + strandat 65.747 kb on + strandat 65.747 kb on + strandat 65.785 kb on - strand, within MMJJ_RS00340at 65.912 kb on + strand, within MMJJ_RS00340at 65.959 kb on + strand, within MMJJ_RS00340at 65.959 kb on + strand, within MMJJ_RS00340at 65.962 kb on + strand, within MMJJ_RS00340at 66.141 kb on - strand, within MMJJ_RS00340at 66.142 kb on + strand, within MMJJ_RS00340at 66.156 kb on - strand, within MMJJ_RS00340at 66.212 kb on + strand, within MMJJ_RS00340at 66.288 kb on - strandat 66.346 kb on - strandat 66.346 kb on - strandat 66.347 kb on + strandat 66.350 kb on - strandat 66.351 kb on + strandat 66.377 kb on - strandat 66.377 kb on - strandat 66.378 kb on + strandat 66.462 kb on - strand, within MMJJ_RS00345at 66.463 kb on + strand, within MMJJ_RS00345at 66.580 kb on + strand, within MMJJ_RS00345at 66.710 kb on - strand, within MMJJ_RS00345at 66.717 kb on - strand, within MMJJ_RS00345at 66.718 kb on + strand, within MMJJ_RS00345at 66.719 kb on - strand, within MMJJ_RS00345at 66.719 kb on - strand, within MMJJ_RS00345at 66.719 kb on - strand, within MMJJ_RS00345at 66.720 kb on + strand, within MMJJ_RS00345at 66.720 kb on + strand, within MMJJ_RS00345at 66.720 kb on + strand, within MMJJ_RS00345at 66.720 kb on + strand, within MMJJ_RS00345at 66.723 kb on - strand, within MMJJ_RS00345at 66.723 kb on - strand, within MMJJ_RS00345at 66.723 kb on - strand, within MMJJ_RS00345at 66.724 kb on + strand, within MMJJ_RS00345at 66.818 kb on - strand, within MMJJ_RS00345at 66.818 kb on - strand, within MMJJ_RS00345at 66.827 kb on - strand, within MMJJ_RS00345at 66.900 kb on - strand, within MMJJ_RS00345at 66.904 kb on + strand, within MMJJ_RS00345at 66.904 kb on + strand, within MMJJ_RS00345at 66.950 kb on - strand, within MMJJ_RS00345at 66.951 kb on + strand, within MMJJ_RS00345at 66.954 kb on - strand, within MMJJ_RS00345at 66.961 kb on + strand, within MMJJ_RS00345at 67.041 kb on - strand, within MMJJ_RS00345at 67.041 kb on - strand, within MMJJ_RS00345at 67.041 kb on - strand, within MMJJ_RS00345at 67.041 kb on - strand, within MMJJ_RS00345at 67.042 kb on + strand, within MMJJ_RS00345at 67.047 kb on - strandat 67.047 kb on - strandat 67.048 kb on + strandat 67.110 kb on + strandat 67.171 kb on + strandat 67.199 kb on - strandat 67.199 kb on - strandat 67.200 kb on + strandat 67.200 kb on + strandat 67.221 kb on + strand, within MMJJ_RS00350at 67.234 kb on - strand, within MMJJ_RS00350at 67.235 kb on + strand, within MMJJ_RS00350at 67.245 kb on + strand, within MMJJ_RS00350at 67.256 kb on - strand, within MMJJ_RS00350at 67.256 kb on - strand, within MMJJ_RS00350at 67.257 kb on + strand, within MMJJ_RS00350at 67.257 kb on + strand, within MMJJ_RS00350at 67.257 kb on + strand, within MMJJ_RS00350at 67.276 kb on + strand, within MMJJ_RS00350at 67.296 kb on + strand, within MMJJ_RS00350

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
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64,747 - MMJJ_RS00330 0.58 -0.7
64,777 - MMJJ_RS00330 0.60 +0.7
64,808 - MMJJ_RS00330 0.63 -1.1
64,808 - MMJJ_RS00330 0.63 +0.8
64,809 + MMJJ_RS00330 0.63 -0.6
64,931 - MMJJ_RS00330 0.74 -0.4
64,932 + MMJJ_RS00330 0.74 +0.0
64,936 - MMJJ_RS00330 0.75 +0.6
65,012 + MMJJ_RS00330 0.82 -1.3
65,054 + MMJJ_RS00330 0.86 +1.0
65,058 + MMJJ_RS00330 0.86 -0.3
65,186 - -0.3
65,231 - +0.3
65,305 + MMJJ_RS00335 0.11 -1.3
65,309 - MMJJ_RS00335 0.12 +0.7
65,338 - MMJJ_RS00335 0.18 -0.5
65,338 - MMJJ_RS00335 0.18 -1.5
65,404 + MMJJ_RS00335 0.33 -0.4
65,427 - MMJJ_RS00335 0.39 -1.6
65,463 - MMJJ_RS00335 0.47 -1.1
65,463 - MMJJ_RS00335 0.47 -0.2
65,464 + MMJJ_RS00335 0.47 +0.4
65,542 + MMJJ_RS00335 0.64 -0.1
65,562 + MMJJ_RS00335 0.69 -0.6
65,637 - MMJJ_RS00335 0.86 -0.7
65,680 + +0.6
65,721 - -0.9
65,721 - -0.3
65,722 + +0.2
65,726 + -0.7
65,726 + +1.7
65,726 + -0.1
65,734 + -0.5
65,746 - -0.7
65,746 - -1.9
65,747 + +2.0
65,747 + -1.0
65,747 + -0.4
65,785 - MMJJ_RS00340 0.13 -0.4
65,912 + MMJJ_RS00340 0.34 +0.1
65,959 + MMJJ_RS00340 0.42 -0.5
65,959 + MMJJ_RS00340 0.42 -0.5
65,962 + MMJJ_RS00340 0.42 -1.3
66,141 - MMJJ_RS00340 0.72 -1.4
66,142 + MMJJ_RS00340 0.72 -0.1
66,156 - MMJJ_RS00340 0.75 -2.4
66,212 + MMJJ_RS00340 0.84 +0.1
66,288 - -0.2
66,346 - -0.6
66,346 - +0.6
66,347 + +0.1
66,350 - -0.5
66,351 + +1.0
66,377 - +1.6
66,377 - -0.8
66,378 + +0.8
66,462 - MMJJ_RS00345 0.18 -0.0
66,463 + MMJJ_RS00345 0.18 -1.8
66,580 + MMJJ_RS00345 0.33 +0.2
66,710 - MMJJ_RS00345 0.49 +0.6
66,717 - MMJJ_RS00345 0.49 -0.5
66,718 + MMJJ_RS00345 0.50 +0.3
66,719 - MMJJ_RS00345 0.50 -0.2
66,719 - MMJJ_RS00345 0.50 -1.5
66,719 - MMJJ_RS00345 0.50 -1.2
66,720 + MMJJ_RS00345 0.50 -1.4
66,720 + MMJJ_RS00345 0.50 -0.1
66,720 + MMJJ_RS00345 0.50 -1.1
66,720 + MMJJ_RS00345 0.50 -2.1
66,723 - MMJJ_RS00345 0.50 -2.9
66,723 - MMJJ_RS00345 0.50 -1.5
66,723 - MMJJ_RS00345 0.50 -0.6
66,724 + MMJJ_RS00345 0.50 -0.4
66,818 - MMJJ_RS00345 0.62 +1.6
66,818 - MMJJ_RS00345 0.62 -0.9
66,827 - MMJJ_RS00345 0.63 -1.9
66,900 - MMJJ_RS00345 0.72 -0.1
66,904 + MMJJ_RS00345 0.72 +0.9
66,904 + MMJJ_RS00345 0.72 -0.8
66,950 - MMJJ_RS00345 0.78 -0.6
66,951 + MMJJ_RS00345 0.78 -1.9
66,954 - MMJJ_RS00345 0.79 +0.3
66,961 + MMJJ_RS00345 0.79 -1.8
67,041 - MMJJ_RS00345 0.89 -1.2
67,041 - MMJJ_RS00345 0.89 -0.1
67,041 - MMJJ_RS00345 0.89 -0.8
67,041 - MMJJ_RS00345 0.89 -2.0
67,042 + MMJJ_RS00345 0.89 -1.2
67,047 - +0.2
67,047 - -3.5
67,048 + +4.1
67,110 + -0.7
67,171 + -0.4
67,199 - -0.1
67,199 - -1.0
67,200 + -0.7
67,200 + -1.4
67,221 + MMJJ_RS00350 0.13 -0.5
67,234 - MMJJ_RS00350 0.15 -0.7
67,235 + MMJJ_RS00350 0.15 +1.3
67,245 + MMJJ_RS00350 0.17 -0.4
67,256 - MMJJ_RS00350 0.18 -0.8
67,256 - MMJJ_RS00350 0.18 +0.8
67,257 + MMJJ_RS00350 0.19 -0.9
67,257 + MMJJ_RS00350 0.19 -0.1
67,257 + MMJJ_RS00350 0.19 -0.6
67,276 + MMJJ_RS00350 0.22 -0.7
67,296 + MMJJ_RS00350 0.25 -0.3

Or see this region's nucleotide sequence