Experiment: R2A with Cobalt chloride 0.08 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS10605 and CA265_RS10610 are separated by 158 nucleotides CA265_RS10610 and CA265_RS10615 overlap by 12 nucleotides CA265_RS10615 and CA265_RS10620 are separated by 98 nucleotides CA265_RS10620 and CA265_RS10625 are separated by 59 nucleotides
CA265_RS10605: CA265_RS10605 - hypothetical protein, at 2,622,990 to 2,623,589
_RS10605
CA265_RS10610: CA265_RS10610 - hypothetical protein, at 2,623,748 to 2,624,275
_RS10610
CA265_RS10615: CA265_RS10615 - diacylglycerol kinase, at 2,624,264 to 2,624,635
_RS10615
CA265_RS10620: CA265_RS10620 - hypothetical protein, at 2,624,734 to 2,625,330
_RS10620
CA265_RS10625: CA265_RS10625 - beta-N-acetylhexosaminidase, at 2,625,390 to 2,627,300
_RS10625
Position (kb)
2624
2625 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2623.594 kb on - strand at 2623.665 kb on + strand at 2623.874 kb on + strand, within CA265_RS10610 at 2623.875 kb on - strand, within CA265_RS10610 at 2623.875 kb on - strand, within CA265_RS10610 at 2623.876 kb on + strand, within CA265_RS10610 at 2623.877 kb on - strand, within CA265_RS10610 at 2623.877 kb on - strand, within CA265_RS10610 at 2623.877 kb on - strand at 2623.880 kb on + strand, within CA265_RS10610 at 2623.881 kb on - strand, within CA265_RS10610 at 2623.915 kb on + strand, within CA265_RS10610 at 2623.916 kb on - strand, within CA265_RS10610 at 2623.916 kb on - strand, within CA265_RS10610 at 2623.933 kb on - strand, within CA265_RS10610 at 2623.959 kb on + strand, within CA265_RS10610 at 2624.004 kb on + strand, within CA265_RS10610 at 2624.005 kb on - strand, within CA265_RS10610 at 2624.030 kb on - strand, within CA265_RS10610 at 2624.038 kb on + strand, within CA265_RS10610 at 2624.039 kb on - strand, within CA265_RS10610 at 2624.050 kb on - strand, within CA265_RS10610 at 2624.110 kb on - strand, within CA265_RS10610 at 2624.158 kb on + strand, within CA265_RS10610 at 2624.159 kb on - strand, within CA265_RS10610 at 2624.162 kb on + strand, within CA265_RS10610 at 2624.194 kb on + strand, within CA265_RS10610 at 2624.195 kb on - strand, within CA265_RS10610 at 2624.221 kb on + strand, within CA265_RS10610 at 2624.293 kb on + strand at 2624.294 kb on - strand at 2624.362 kb on + strand, within CA265_RS10615 at 2624.377 kb on - strand, within CA265_RS10615 at 2624.432 kb on + strand, within CA265_RS10615 at 2624.435 kb on + strand, within CA265_RS10615 at 2624.438 kb on - strand, within CA265_RS10615 at 2624.457 kb on - strand, within CA265_RS10615 at 2624.513 kb on + strand, within CA265_RS10615 at 2624.587 kb on - strand, within CA265_RS10615 at 2624.612 kb on - strand at 2624.654 kb on + strand at 2624.654 kb on + strand at 2624.655 kb on - strand at 2624.707 kb on + strand at 2624.714 kb on + strand at 2624.752 kb on - strand at 2625.019 kb on - strand, within CA265_RS10620 at 2625.052 kb on - strand, within CA265_RS10620 at 2625.078 kb on + strand, within CA265_RS10620 at 2625.079 kb on - strand, within CA265_RS10620 at 2625.079 kb on - strand, within CA265_RS10620 at 2625.081 kb on - strand, within CA265_RS10620 at 2625.126 kb on + strand, within CA265_RS10620 at 2625.167 kb on - strand, within CA265_RS10620 at 2625.184 kb on + strand, within CA265_RS10620 at 2625.187 kb on - strand, within CA265_RS10620 at 2625.289 kb on - strand at 2625.307 kb on + strand at 2625.312 kb on + strand at 2625.313 kb on - strand at 2625.315 kb on - strand at 2625.333 kb on - strand at 2625.364 kb on + strand at 2625.392 kb on - strand at 2625.450 kb on + strand at 2625.478 kb on - strand at 2625.478 kb on - strand at 2625.525 kb on - strand at 2625.538 kb on + strand at 2625.540 kb on + strand at 2625.541 kb on - strand at 2625.568 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.08 mM remove 2,623,594 - +1.3 2,623,665 + +0.8 2,623,874 + CA265_RS10610 0.24 -3.1 2,623,875 - CA265_RS10610 0.24 -0.2 2,623,875 - CA265_RS10610 0.24 -0.4 2,623,876 + CA265_RS10610 0.24 +0.5 2,623,877 - CA265_RS10610 0.24 -0.2 2,623,877 - CA265_RS10610 0.24 -0.8 2,623,877 - +1.4 2,623,880 + CA265_RS10610 0.25 +0.7 2,623,881 - CA265_RS10610 0.25 -0.2 2,623,915 + CA265_RS10610 0.32 +0.0 2,623,916 - CA265_RS10610 0.32 -0.1 2,623,916 - CA265_RS10610 0.32 -0.2 2,623,933 - CA265_RS10610 0.35 -0.9 2,623,959 + CA265_RS10610 0.40 +0.8 2,624,004 + CA265_RS10610 0.48 -0.8 2,624,005 - CA265_RS10610 0.49 -2.1 2,624,030 - CA265_RS10610 0.53 -0.1 2,624,038 + CA265_RS10610 0.55 -0.1 2,624,039 - CA265_RS10610 0.55 -0.1 2,624,050 - CA265_RS10610 0.57 -0.1 2,624,110 - CA265_RS10610 0.69 -0.3 2,624,158 + CA265_RS10610 0.78 -0.0 2,624,159 - CA265_RS10610 0.78 -0.5 2,624,162 + CA265_RS10610 0.78 -0.1 2,624,194 + CA265_RS10610 0.84 -0.4 2,624,195 - CA265_RS10610 0.85 +0.5 2,624,221 + CA265_RS10610 0.90 -0.1 2,624,293 + -0.9 2,624,294 - +1.0 2,624,362 + CA265_RS10615 0.26 -0.2 2,624,377 - CA265_RS10615 0.30 +0.2 2,624,432 + CA265_RS10615 0.45 +1.3 2,624,435 + CA265_RS10615 0.46 +0.3 2,624,438 - CA265_RS10615 0.47 +1.1 2,624,457 - CA265_RS10615 0.52 -1.0 2,624,513 + CA265_RS10615 0.67 +0.0 2,624,587 - CA265_RS10615 0.87 +0.3 2,624,612 - -0.7 2,624,654 + +0.2 2,624,654 + +0.3 2,624,655 - +0.4 2,624,707 + +0.7 2,624,714 + -0.1 2,624,752 - -0.6 2,625,019 - CA265_RS10620 0.48 +0.3 2,625,052 - CA265_RS10620 0.53 +0.3 2,625,078 + CA265_RS10620 0.58 +0.1 2,625,079 - CA265_RS10620 0.58 -0.3 2,625,079 - CA265_RS10620 0.58 -0.8 2,625,081 - CA265_RS10620 0.58 -0.3 2,625,126 + CA265_RS10620 0.66 -0.7 2,625,167 - CA265_RS10620 0.73 -0.3 2,625,184 + CA265_RS10620 0.75 -0.4 2,625,187 - CA265_RS10620 0.76 -1.1 2,625,289 - -0.1 2,625,307 + -0.5 2,625,312 + +0.5 2,625,313 - -1.0 2,625,315 - -0.5 2,625,333 - -0.6 2,625,364 + -1.7 2,625,392 - +0.2 2,625,450 + -0.2 2,625,478 - -0.7 2,625,478 - +0.5 2,625,525 - +1.0 2,625,538 + +0.7 2,625,540 + +0.1 2,625,541 - +0.2 2,625,568 - -1.3
Or see this region's nucleotide sequence