Experiment: R2A with Cobalt chloride 0.08 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS06295 and CA265_RS06300 are separated by 359 nucleotides CA265_RS06300 and CA265_RS06305 are separated by 295 nucleotides
CA265_RS06295: CA265_RS06295 - endonuclease MutS2, at 1,545,184 to 1,547,562
_RS06295
CA265_RS06300: CA265_RS06300 - glucose dehydrogenase, at 1,547,922 to 1,549,010
_RS06300
CA265_RS06305: CA265_RS06305 - succinyl-CoA--3-ketoacid-CoA transferase, at 1,549,306 to 1,550,010
_RS06305
Position (kb)
1547
1548
1549
1550 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1546.935 kb on + strand, within CA265_RS06295 at 1546.935 kb on + strand, within CA265_RS06295 at 1546.936 kb on - strand, within CA265_RS06295 at 1546.969 kb on - strand, within CA265_RS06295 at 1547.010 kb on - strand, within CA265_RS06295 at 1547.244 kb on + strand, within CA265_RS06295 at 1547.245 kb on - strand, within CA265_RS06295 at 1547.375 kb on + strand at 1547.474 kb on - strand at 1547.482 kb on - strand at 1547.487 kb on + strand at 1547.487 kb on + strand at 1547.669 kb on + strand at 1547.683 kb on - strand at 1547.686 kb on - strand at 1547.724 kb on + strand at 1547.725 kb on - strand at 1547.786 kb on - strand at 1547.786 kb on - strand at 1547.801 kb on + strand at 1547.801 kb on + strand at 1547.832 kb on + strand at 1547.933 kb on - strand at 1547.936 kb on - strand at 1547.951 kb on - strand at 1547.951 kb on - strand at 1547.991 kb on - strand at 1548.020 kb on - strand at 1548.020 kb on - strand at 1548.053 kb on - strand, within CA265_RS06300 at 1548.075 kb on + strand, within CA265_RS06300 at 1548.122 kb on + strand, within CA265_RS06300 at 1548.151 kb on + strand, within CA265_RS06300 at 1548.151 kb on + strand, within CA265_RS06300 at 1548.152 kb on - strand, within CA265_RS06300 at 1548.160 kb on + strand, within CA265_RS06300 at 1548.161 kb on - strand, within CA265_RS06300 at 1548.164 kb on + strand, within CA265_RS06300 at 1548.165 kb on - strand, within CA265_RS06300 at 1548.217 kb on + strand, within CA265_RS06300 at 1548.217 kb on + strand, within CA265_RS06300 at 1548.218 kb on - strand, within CA265_RS06300 at 1548.249 kb on + strand, within CA265_RS06300 at 1548.249 kb on + strand, within CA265_RS06300 at 1548.250 kb on - strand, within CA265_RS06300 at 1548.276 kb on + strand, within CA265_RS06300 at 1548.276 kb on + strand, within CA265_RS06300 at 1548.293 kb on - strand, within CA265_RS06300 at 1548.293 kb on - strand, within CA265_RS06300 at 1548.432 kb on - strand, within CA265_RS06300 at 1548.460 kb on + strand, within CA265_RS06300 at 1548.475 kb on + strand, within CA265_RS06300 at 1548.476 kb on - strand, within CA265_RS06300 at 1548.481 kb on + strand, within CA265_RS06300 at 1548.487 kb on + strand, within CA265_RS06300 at 1548.497 kb on + strand, within CA265_RS06300 at 1548.581 kb on + strand, within CA265_RS06300 at 1548.627 kb on - strand, within CA265_RS06300 at 1548.652 kb on - strand, within CA265_RS06300 at 1548.666 kb on - strand, within CA265_RS06300 at 1548.680 kb on + strand, within CA265_RS06300 at 1548.716 kb on + strand, within CA265_RS06300 at 1548.790 kb on - strand, within CA265_RS06300 at 1548.790 kb on - strand, within CA265_RS06300 at 1548.909 kb on - strand at 1548.909 kb on - strand at 1548.912 kb on + strand at 1549.008 kb on + strand at 1549.008 kb on + strand at 1549.008 kb on + strand at 1549.146 kb on - strand at 1549.152 kb on - strand at 1549.152 kb on - strand at 1549.314 kb on + strand at 1549.314 kb on + strand at 1549.315 kb on - strand at 1549.315 kb on - strand at 1549.353 kb on - strand at 1549.464 kb on + strand, within CA265_RS06305 at 1549.537 kb on + strand, within CA265_RS06305 at 1549.538 kb on - strand, within CA265_RS06305 at 1549.538 kb on - strand, within CA265_RS06305 at 1549.592 kb on + strand at 1549.616 kb on + strand, within CA265_RS06305 at 1549.770 kb on + strand, within CA265_RS06305 at 1549.771 kb on - strand, within CA265_RS06305 at 1549.914 kb on + strand, within CA265_RS06305 at 1549.915 kb on - strand, within CA265_RS06305 at 1549.920 kb on + strand, within CA265_RS06305 at 1549.993 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Cobalt chloride 0.08 mM remove 1,546,935 + CA265_RS06295 0.74 -0.2 1,546,935 + CA265_RS06295 0.74 -1.3 1,546,936 - CA265_RS06295 0.74 -3.2 1,546,969 - CA265_RS06295 0.75 -2.2 1,547,010 - CA265_RS06295 0.77 -2.3 1,547,244 + CA265_RS06295 0.87 -2.3 1,547,245 - CA265_RS06295 0.87 -1.5 1,547,375 + -3.3 1,547,474 - -1.5 1,547,482 - -1.6 1,547,487 + -4.3 1,547,487 + -1.8 1,547,669 + +0.5 1,547,683 - -0.0 1,547,686 - -0.1 1,547,724 + -1.1 1,547,725 - +0.6 1,547,786 - -1.8 1,547,786 - -2.8 1,547,801 + +1.7 1,547,801 + -0.5 1,547,832 + -0.5 1,547,933 - +0.2 1,547,936 - -3.2 1,547,951 - +0.1 1,547,951 - +0.2 1,547,991 - -0.4 1,548,020 - -0.9 1,548,020 - -1.2 1,548,053 - CA265_RS06300 0.12 -0.6 1,548,075 + CA265_RS06300 0.14 -1.7 1,548,122 + CA265_RS06300 0.18 -0.6 1,548,151 + CA265_RS06300 0.21 +0.2 1,548,151 + CA265_RS06300 0.21 -0.0 1,548,152 - CA265_RS06300 0.21 -2.8 1,548,160 + CA265_RS06300 0.22 -0.1 1,548,161 - CA265_RS06300 0.22 -1.2 1,548,164 + CA265_RS06300 0.22 +0.5 1,548,165 - CA265_RS06300 0.22 -1.0 1,548,217 + CA265_RS06300 0.27 +1.0 1,548,217 + CA265_RS06300 0.27 +0.0 1,548,218 - CA265_RS06300 0.27 +0.1 1,548,249 + CA265_RS06300 0.30 +0.5 1,548,249 + CA265_RS06300 0.30 -0.7 1,548,250 - CA265_RS06300 0.30 +1.2 1,548,276 + CA265_RS06300 0.33 -0.8 1,548,276 + CA265_RS06300 0.33 +0.0 1,548,293 - CA265_RS06300 0.34 -0.5 1,548,293 - CA265_RS06300 0.34 -0.9 1,548,432 - CA265_RS06300 0.47 -0.2 1,548,460 + CA265_RS06300 0.49 +0.5 1,548,475 + CA265_RS06300 0.51 -0.6 1,548,476 - CA265_RS06300 0.51 +0.3 1,548,481 + CA265_RS06300 0.51 -0.1 1,548,487 + CA265_RS06300 0.52 -1.7 1,548,497 + CA265_RS06300 0.53 -0.4 1,548,581 + CA265_RS06300 0.61 +0.5 1,548,627 - CA265_RS06300 0.65 -1.1 1,548,652 - CA265_RS06300 0.67 -0.1 1,548,666 - CA265_RS06300 0.68 +0.3 1,548,680 + CA265_RS06300 0.70 -0.0 1,548,716 + CA265_RS06300 0.73 +0.1 1,548,790 - CA265_RS06300 0.80 -0.9 1,548,790 - CA265_RS06300 0.80 +0.4 1,548,909 - -3.0 1,548,909 - +0.3 1,548,912 + -0.4 1,549,008 + -0.4 1,549,008 + +0.2 1,549,008 + +0.6 1,549,146 - -1.9 1,549,152 - -1.1 1,549,152 - -1.1 1,549,314 + +1.1 1,549,314 + -0.5 1,549,315 - +0.4 1,549,315 - +1.1 1,549,353 - -0.7 1,549,464 + CA265_RS06305 0.22 -0.1 1,549,537 + CA265_RS06305 0.33 -0.5 1,549,538 - CA265_RS06305 0.33 -0.2 1,549,538 - CA265_RS06305 0.33 +1.9 1,549,592 + +1.1 1,549,616 + CA265_RS06305 0.44 -1.1 1,549,770 + CA265_RS06305 0.66 -0.6 1,549,771 - CA265_RS06305 0.66 +0.3 1,549,914 + CA265_RS06305 0.86 -0.9 1,549,915 - CA265_RS06305 0.86 +0.9 1,549,920 + CA265_RS06305 0.87 +0.2 1,549,993 - +0.2
Or see this region's nucleotide sequence