Strain Fitness in Burkholderia phytofirmans PsJN around BPHYT_RS32225

Experiment: Zinc 0.5 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBPHYT_RS32215 and BPHYT_RS32220 are separated by 116 nucleotidesBPHYT_RS32220 and BPHYT_RS32225 are separated by 237 nucleotidesBPHYT_RS32225 and BPHYT_RS32230 are separated by 214 nucleotides BPHYT_RS32215: BPHYT_RS32215 - AlpA family transcriptional regulator, at 2,798,277 to 2,798,510 _RS32215 BPHYT_RS32220: BPHYT_RS32220 - hypothetical protein, at 2,798,627 to 2,799,163 _RS32220 BPHYT_RS32225: BPHYT_RS32225 - hypothetical protein, at 2,799,401 to 2,799,799 _RS32225 BPHYT_RS32230: BPHYT_RS32230 - fatty acid desaturase, at 2,800,014 to 2,800,958 _RS32230 Position (kb) 2799 2800Strain fitness (log2 ratio) -2 -1 0 1at 2799.346 kb on + strandat 2799.512 kb on - strand, within BPHYT_RS32225at 2799.730 kb on - strand, within BPHYT_RS32225at 2799.736 kb on - strand, within BPHYT_RS32225at 2799.828 kb on + strandat 2799.893 kb on + strandat 2799.957 kb on + strandat 2799.958 kb on - strandat 2800.073 kb on - strandat 2800.157 kb on - strand, within BPHYT_RS32230at 2800.157 kb on - strand, within BPHYT_RS32230at 2800.186 kb on + strand, within BPHYT_RS32230at 2800.203 kb on - strand, within BPHYT_RS32230at 2800.479 kb on - strand, within BPHYT_RS32230at 2800.729 kb on - strand, within BPHYT_RS32230

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Per-strain Table

Position Strand Gene LocusTag Fraction Zinc 0.5 mM
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2,799,346 + +0.2
2,799,512 - BPHYT_RS32225 0.28 -2.3
2,799,730 - BPHYT_RS32225 0.82 -0.6
2,799,736 - BPHYT_RS32225 0.84 -0.4
2,799,828 + +0.6
2,799,893 + -0.4
2,799,957 + +0.4
2,799,958 - -0.5
2,800,073 - +0.1
2,800,157 - BPHYT_RS32230 0.15 +0.3
2,800,157 - BPHYT_RS32230 0.15 -0.0
2,800,186 + BPHYT_RS32230 0.18 -0.1
2,800,203 - BPHYT_RS32230 0.20 +0.0
2,800,479 - BPHYT_RS32230 0.49 +0.0
2,800,729 - BPHYT_RS32230 0.76 -0.3

Or see this region's nucleotide sequence