Experiment: Cobalt chloride 0.16 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_560 and Pf6N2E2_561 overlap by 4 nucleotides Pf6N2E2_561 and Pf6N2E2_562 overlap by 4 nucleotides
Pf6N2E2_560 - IcmF-related protein, at 630,724 to 634,548
_560
Pf6N2E2_561 - Uncharacterized protein ImpA, at 634,545 to 635,627
_561
Pf6N2E2_562 - D-3-phosphoglycerate dehydrogenase (EC 1.1.1.95), at 635,624 to 636,577
_562
Position (kb) 634
635
636 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 633.719 kb on - strand, within Pf6N2E2_560 at 633.766 kb on + strand, within Pf6N2E2_560 at 633.767 kb on - strand, within Pf6N2E2_560 at 633.767 kb on - strand, within Pf6N2E2_560 at 633.779 kb on - strand, within Pf6N2E2_560 at 633.964 kb on - strand, within Pf6N2E2_560 at 633.987 kb on - strand, within Pf6N2E2_560 at 634.261 kb on + strand at 634.333 kb on + strand at 634.334 kb on - strand at 634.823 kb on + strand, within Pf6N2E2_561 at 634.824 kb on - strand, within Pf6N2E2_561 at 635.241 kb on + strand, within Pf6N2E2_561 at 635.241 kb on + strand, within Pf6N2E2_561 at 635.242 kb on - strand, within Pf6N2E2_561 at 635.266 kb on - strand, within Pf6N2E2_561 at 635.266 kb on - strand, within Pf6N2E2_561 at 635.459 kb on - strand, within Pf6N2E2_561 at 635.459 kb on - strand, within Pf6N2E2_561 at 635.486 kb on + strand, within Pf6N2E2_561 at 635.708 kb on + strand at 635.708 kb on + strand at 636.036 kb on + strand, within Pf6N2E2_562 at 636.069 kb on + strand, within Pf6N2E2_562 at 636.070 kb on - strand, within Pf6N2E2_562 at 636.076 kb on + strand, within Pf6N2E2_562 at 636.222 kb on - strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.335 kb on + strand, within Pf6N2E2_562 at 636.336 kb on - strand, within Pf6N2E2_562 at 636.336 kb on - strand, within Pf6N2E2_562 at 636.336 kb on - strand, within Pf6N2E2_562 at 636.534 kb on + strand at 636.535 kb on - strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.590 kb on + strand at 636.591 kb on - strand at 636.591 kb on - strand at 636.612 kb on + strand at 636.613 kb on - strand at 636.613 kb on - strand at 636.627 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.16 mM remove 633,719 - Pf6N2E2_560 0.78 +0.3 633,766 + Pf6N2E2_560 0.80 -2.5 633,767 - Pf6N2E2_560 0.80 +0.7 633,767 - Pf6N2E2_560 0.80 +0.5 633,779 - Pf6N2E2_560 0.80 +0.3 633,964 - Pf6N2E2_560 0.85 +0.1 633,987 - Pf6N2E2_560 0.85 +0.8 634,261 + -3.2 634,333 + -1.1 634,334 - +0.6 634,823 + Pf6N2E2_561 0.26 -1.2 634,824 - Pf6N2E2_561 0.26 -0.1 635,241 + Pf6N2E2_561 0.64 -3.6 635,241 + Pf6N2E2_561 0.64 -0.1 635,242 - Pf6N2E2_561 0.64 -0.0 635,266 - Pf6N2E2_561 0.67 -1.3 635,266 - Pf6N2E2_561 0.67 -0.2 635,459 - Pf6N2E2_561 0.84 -3.3 635,459 - Pf6N2E2_561 0.84 +0.1 635,486 + Pf6N2E2_561 0.87 -0.4 635,708 + -0.7 635,708 + -1.2 636,036 + Pf6N2E2_562 0.43 -1.8 636,069 + Pf6N2E2_562 0.47 -0.3 636,070 - Pf6N2E2_562 0.47 +0.9 636,076 + Pf6N2E2_562 0.47 -0.8 636,222 - Pf6N2E2_562 0.63 +1.0 636,335 + Pf6N2E2_562 0.75 -0.3 636,335 + Pf6N2E2_562 0.75 -0.6 636,335 + Pf6N2E2_562 0.75 -0.5 636,335 + Pf6N2E2_562 0.75 -1.6 636,336 - Pf6N2E2_562 0.75 -1.6 636,336 - Pf6N2E2_562 0.75 -0.6 636,336 - Pf6N2E2_562 0.75 -0.5 636,534 + -0.4 636,535 - +0.2 636,590 + -0.1 636,590 + +0.4 636,590 + -0.1 636,590 + -0.2 636,590 + -1.2 636,591 - -1.2 636,591 - -0.5 636,612 + +0.0 636,613 - +0.4 636,613 - +0.2 636,627 + -1.3
Or see this region's nucleotide sequence