Experiment: Cobalt chloride 0.16 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3079 and Pf6N2E2_3080 overlap by 4 nucleotides Pf6N2E2_3080 and Pf6N2E2_3081 are separated by 126 nucleotides Pf6N2E2_3081 and Pf6N2E2_3082 are separated by 44 nucleotides Pf6N2E2_3082 and Pf6N2E2_3083 overlap by 12 nucleotides
Pf6N2E2_3079 - Cysteine desulfurase CsdA-CsdE (EC 2.8.1.7), main protein CsdA, at 3,567,412 to 3,568,617
_3079
Pf6N2E2_3080 - Cysteine desulfurase CsdA-CsdE, sulfur acceptor protein CsdE, at 3,568,614 to 3,569,021
_3080
Pf6N2E2_3081 - HesA/MoeB/ThiF family protein related to EC-YgdL, at 3,569,148 to 3,569,966
_3081
Pf6N2E2_3082 - hypothetical protein, at 3,570,011 to 3,570,190
_3082
Pf6N2E2_3083 - N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18), at 3,570,179 to 3,571,330
_3083
Position (kb)
3569
3570 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3568.277 kb on - strand, within Pf6N2E2_3079 at 3568.277 kb on - strand, within Pf6N2E2_3079 at 3568.333 kb on + strand, within Pf6N2E2_3079 at 3568.333 kb on + strand, within Pf6N2E2_3079 at 3568.333 kb on + strand, within Pf6N2E2_3079 at 3568.334 kb on - strand, within Pf6N2E2_3079 at 3568.549 kb on + strand at 3568.549 kb on + strand at 3568.549 kb on + strand at 3568.549 kb on + strand at 3568.968 kb on - strand, within Pf6N2E2_3080 at 3569.201 kb on + strand at 3569.380 kb on + strand, within Pf6N2E2_3081 at 3569.380 kb on + strand, within Pf6N2E2_3081 at 3569.381 kb on - strand, within Pf6N2E2_3081 at 3569.381 kb on - strand, within Pf6N2E2_3081 at 3569.433 kb on + strand, within Pf6N2E2_3081 at 3569.433 kb on + strand, within Pf6N2E2_3081 at 3569.433 kb on + strand, within Pf6N2E2_3081 at 3569.434 kb on - strand, within Pf6N2E2_3081 at 3569.434 kb on - strand, within Pf6N2E2_3081 at 3569.626 kb on + strand, within Pf6N2E2_3081 at 3569.626 kb on + strand, within Pf6N2E2_3081 at 3569.626 kb on + strand, within Pf6N2E2_3081 at 3569.627 kb on - strand, within Pf6N2E2_3081 at 3569.627 kb on - strand, within Pf6N2E2_3081 at 3569.918 kb on - strand at 3569.955 kb on - strand at 3569.989 kb on + strand at 3569.989 kb on + strand at 3569.990 kb on - strand at 3569.990 kb on - strand at 3569.990 kb on - strand at 3569.991 kb on + strand at 3569.992 kb on - strand at 3569.992 kb on - strand at 3569.992 kb on - strand at 3569.992 kb on - strand at 3570.100 kb on + strand, within Pf6N2E2_3082 at 3570.101 kb on - strand, within Pf6N2E2_3082 at 3570.102 kb on + strand, within Pf6N2E2_3082 at 3570.102 kb on + strand, within Pf6N2E2_3082 at 3570.103 kb on - strand, within Pf6N2E2_3082 at 3570.107 kb on + strand, within Pf6N2E2_3082 at 3570.107 kb on + strand, within Pf6N2E2_3082 at 3570.107 kb on + strand, within Pf6N2E2_3082 at 3570.107 kb on + strand, within Pf6N2E2_3082 at 3570.141 kb on + strand, within Pf6N2E2_3082 at 3570.161 kb on + strand, within Pf6N2E2_3082 at 3570.161 kb on + strand, within Pf6N2E2_3082
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.16 mM remove 3,568,277 - Pf6N2E2_3079 0.72 -1.0 3,568,277 - Pf6N2E2_3079 0.72 -1.7 3,568,333 + Pf6N2E2_3079 0.76 -0.1 3,568,333 + Pf6N2E2_3079 0.76 +1.0 3,568,333 + Pf6N2E2_3079 0.76 -1.5 3,568,334 - Pf6N2E2_3079 0.76 -1.6 3,568,549 + -0.9 3,568,549 + -0.8 3,568,549 + -0.9 3,568,549 + +0.0 3,568,968 - Pf6N2E2_3080 0.87 -1.0 3,569,201 + -0.2 3,569,380 + Pf6N2E2_3081 0.28 -0.7 3,569,380 + Pf6N2E2_3081 0.28 -1.1 3,569,381 - Pf6N2E2_3081 0.28 -0.2 3,569,381 - Pf6N2E2_3081 0.28 -0.7 3,569,433 + Pf6N2E2_3081 0.35 +0.0 3,569,433 + Pf6N2E2_3081 0.35 -0.7 3,569,433 + Pf6N2E2_3081 0.35 +2.3 3,569,434 - Pf6N2E2_3081 0.35 -0.6 3,569,434 - Pf6N2E2_3081 0.35 +0.1 3,569,626 + Pf6N2E2_3081 0.58 -1.0 3,569,626 + Pf6N2E2_3081 0.58 -1.8 3,569,626 + Pf6N2E2_3081 0.58 -0.1 3,569,627 - Pf6N2E2_3081 0.58 -0.5 3,569,627 - Pf6N2E2_3081 0.58 -0.1 3,569,918 - -0.9 3,569,955 - -0.3 3,569,989 + +0.1 3,569,989 + -1.3 3,569,990 - +0.7 3,569,990 - -0.1 3,569,990 - -0.1 3,569,991 + +0.5 3,569,992 - -0.3 3,569,992 - -0.6 3,569,992 - -0.3 3,569,992 - +0.2 3,570,100 + Pf6N2E2_3082 0.49 +0.8 3,570,101 - Pf6N2E2_3082 0.50 -0.5 3,570,102 + Pf6N2E2_3082 0.51 -0.1 3,570,102 + Pf6N2E2_3082 0.51 -0.6 3,570,103 - Pf6N2E2_3082 0.51 -1.2 3,570,107 + Pf6N2E2_3082 0.53 -0.9 3,570,107 + Pf6N2E2_3082 0.53 -0.0 3,570,107 + Pf6N2E2_3082 0.53 +0.5 3,570,107 + Pf6N2E2_3082 0.53 -0.3 3,570,141 + Pf6N2E2_3082 0.72 -0.4 3,570,161 + Pf6N2E2_3082 0.83 -0.1 3,570,161 + Pf6N2E2_3082 0.83 -1.4
Or see this region's nucleotide sequence