Experiment: Cobalt chloride 0.16 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2233 and Pf6N2E2_2234 are separated by 260 nucleotides Pf6N2E2_2234 and Pf6N2E2_2235 are separated by 170 nucleotides Pf6N2E2_2235 and Pf6N2E2_2236 are separated by 124 nucleotides
Pf6N2E2_2233 - Shikimate 5-dehydrogenase I gamma (EC 1.1.1.25), at 2,645,420 to 2,646,241
_2233
Pf6N2E2_2234 - Virulence protein, at 2,646,502 to 2,646,852
_2234
Pf6N2E2_2235 - Xanthine permease, at 2,647,023 to 2,648,543
_2235
Pf6N2E2_2236 - N-hydroxyarylamine O-acetyltransferase (EC 2.3.1.118), at 2,648,668 to 2,649,492
_2236
Position (kb)
2647
2648
2649 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2646.113 kb on - strand, within Pf6N2E2_2233 at 2646.113 kb on - strand, within Pf6N2E2_2233 at 2646.123 kb on + strand, within Pf6N2E2_2233 at 2646.123 kb on + strand, within Pf6N2E2_2233 at 2646.123 kb on + strand, within Pf6N2E2_2233 at 2646.123 kb on + strand, within Pf6N2E2_2233 at 2646.123 kb on + strand, within Pf6N2E2_2233 at 2646.124 kb on - strand, within Pf6N2E2_2233 at 2646.124 kb on - strand, within Pf6N2E2_2233 at 2646.124 kb on - strand, within Pf6N2E2_2233 at 2646.126 kb on + strand, within Pf6N2E2_2233 at 2646.127 kb on - strand, within Pf6N2E2_2233 at 2646.150 kb on + strand, within Pf6N2E2_2233 at 2646.150 kb on + strand, within Pf6N2E2_2233 at 2646.151 kb on - strand, within Pf6N2E2_2233 at 2646.151 kb on - strand, within Pf6N2E2_2233 at 2646.151 kb on - strand, within Pf6N2E2_2233 at 2646.160 kb on + strand at 2646.309 kb on + strand at 2646.309 kb on + strand at 2646.327 kb on + strand at 2646.362 kb on - strand at 2646.379 kb on + strand at 2646.386 kb on + strand at 2646.386 kb on + strand at 2646.386 kb on + strand at 2646.386 kb on + strand at 2646.387 kb on - strand at 2646.387 kb on - strand at 2646.387 kb on - strand at 2646.387 kb on - strand at 2646.387 kb on - strand at 2646.387 kb on - strand at 2646.387 kb on - strand at 2646.390 kb on + strand at 2646.429 kb on + strand at 2646.429 kb on + strand at 2646.429 kb on + strand at 2646.430 kb on - strand at 2646.430 kb on - strand at 2646.430 kb on - strand at 2646.437 kb on - strand at 2646.437 kb on - strand at 2646.667 kb on + strand, within Pf6N2E2_2234 at 2646.667 kb on + strand, within Pf6N2E2_2234 at 2646.667 kb on + strand, within Pf6N2E2_2234 at 2646.667 kb on + strand, within Pf6N2E2_2234 at 2646.667 kb on + strand, within Pf6N2E2_2234 at 2646.667 kb on + strand, within Pf6N2E2_2234 at 2646.667 kb on + strand, within Pf6N2E2_2234 at 2646.667 kb on + strand, within Pf6N2E2_2234 at 2646.667 kb on + strand, within Pf6N2E2_2234 at 2646.668 kb on - strand, within Pf6N2E2_2234 at 2646.668 kb on - strand, within Pf6N2E2_2234 at 2646.668 kb on - strand, within Pf6N2E2_2234 at 2646.668 kb on - strand, within Pf6N2E2_2234 at 2646.668 kb on - strand, within Pf6N2E2_2234 at 2646.668 kb on - strand, within Pf6N2E2_2234 at 2646.685 kb on + strand, within Pf6N2E2_2234 at 2646.685 kb on + strand, within Pf6N2E2_2234 at 2646.685 kb on + strand, within Pf6N2E2_2234 at 2646.685 kb on + strand, within Pf6N2E2_2234 at 2646.685 kb on + strand, within Pf6N2E2_2234 at 2646.685 kb on + strand, within Pf6N2E2_2234 at 2646.685 kb on + strand, within Pf6N2E2_2234 at 2646.706 kb on + strand, within Pf6N2E2_2234 at 2646.706 kb on + strand, within Pf6N2E2_2234 at 2646.707 kb on - strand, within Pf6N2E2_2234 at 2646.707 kb on - strand, within Pf6N2E2_2234 at 2646.709 kb on + strand, within Pf6N2E2_2234 at 2646.709 kb on + strand, within Pf6N2E2_2234 at 2646.709 kb on + strand, within Pf6N2E2_2234 at 2646.710 kb on - strand, within Pf6N2E2_2234 at 2646.730 kb on + strand, within Pf6N2E2_2234 at 2646.730 kb on + strand, within Pf6N2E2_2234 at 2646.731 kb on - strand, within Pf6N2E2_2234 at 2646.734 kb on - strand, within Pf6N2E2_2234 at 2646.734 kb on - strand, within Pf6N2E2_2234 at 2646.751 kb on + strand, within Pf6N2E2_2234 at 2646.751 kb on + strand, within Pf6N2E2_2234 at 2646.751 kb on + strand, within Pf6N2E2_2234 at 2646.751 kb on + strand, within Pf6N2E2_2234 at 2646.751 kb on + strand, within Pf6N2E2_2234 at 2646.751 kb on + strand, within Pf6N2E2_2234 at 2646.751 kb on + strand, within Pf6N2E2_2234 at 2646.751 kb on + strand, within Pf6N2E2_2234 at 2646.751 kb on + strand, within Pf6N2E2_2234 at 2646.751 kb on + strand, within Pf6N2E2_2234 at 2646.751 kb on + strand, within Pf6N2E2_2234 at 2646.751 kb on + strand, within Pf6N2E2_2234 at 2646.752 kb on - strand, within Pf6N2E2_2234 at 2646.752 kb on - strand, within Pf6N2E2_2234 at 2646.752 kb on - strand, within Pf6N2E2_2234 at 2646.752 kb on - strand, within Pf6N2E2_2234 at 2646.752 kb on - strand, within Pf6N2E2_2234 at 2646.752 kb on - strand, within Pf6N2E2_2234 at 2646.755 kb on + strand, within Pf6N2E2_2234 at 2646.755 kb on + strand, within Pf6N2E2_2234 at 2646.756 kb on - strand, within Pf6N2E2_2234 at 2646.769 kb on + strand, within Pf6N2E2_2234 at 2646.769 kb on + strand, within Pf6N2E2_2234 at 2646.769 kb on + strand, within Pf6N2E2_2234 at 2646.769 kb on + strand, within Pf6N2E2_2234 at 2646.770 kb on - strand, within Pf6N2E2_2234 at 2646.770 kb on - strand, within Pf6N2E2_2234 at 2646.772 kb on + strand, within Pf6N2E2_2234 at 2646.772 kb on + strand, within Pf6N2E2_2234 at 2646.790 kb on + strand, within Pf6N2E2_2234 at 2646.791 kb on - strand, within Pf6N2E2_2234 at 2646.793 kb on + strand, within Pf6N2E2_2234 at 2646.811 kb on + strand, within Pf6N2E2_2234 at 2646.812 kb on - strand, within Pf6N2E2_2234 at 2646.812 kb on - strand, within Pf6N2E2_2234 at 2646.814 kb on + strand, within Pf6N2E2_2234 at 2646.814 kb on + strand, within Pf6N2E2_2234 at 2646.814 kb on + strand, within Pf6N2E2_2234 at 2646.814 kb on + strand, within Pf6N2E2_2234 at 2646.814 kb on + strand, within Pf6N2E2_2234 at 2646.814 kb on + strand, within Pf6N2E2_2234 at 2646.815 kb on - strand, within Pf6N2E2_2234 at 2646.815 kb on - strand, within Pf6N2E2_2234 at 2646.815 kb on - strand, within Pf6N2E2_2234 at 2646.815 kb on - strand, within Pf6N2E2_2234 at 2646.815 kb on - strand, within Pf6N2E2_2234 at 2646.818 kb on - strand at 2647.141 kb on + strand at 2647.141 kb on + strand at 2647.142 kb on - strand at 2647.142 kb on - strand at 2647.142 kb on - strand at 2647.142 kb on - strand at 2647.142 kb on - strand at 2647.156 kb on - strand at 2647.156 kb on - strand at 2647.333 kb on + strand, within Pf6N2E2_2235 at 2647.334 kb on - strand, within Pf6N2E2_2235 at 2647.334 kb on - strand, within Pf6N2E2_2235 at 2647.464 kb on + strand, within Pf6N2E2_2235 at 2647.464 kb on + strand, within Pf6N2E2_2235 at 2647.464 kb on + strand, within Pf6N2E2_2235 at 2647.465 kb on - strand, within Pf6N2E2_2235 at 2647.980 kb on + strand, within Pf6N2E2_2235 at 2647.981 kb on - strand, within Pf6N2E2_2235 at 2648.446 kb on + strand at 2648.446 kb on + strand at 2648.446 kb on + strand at 2648.446 kb on + strand at 2648.446 kb on + strand at 2648.447 kb on - strand at 2648.447 kb on - strand at 2648.447 kb on - strand at 2648.474 kb on - strand at 2648.480 kb on + strand at 2648.481 kb on - strand at 2648.610 kb on + strand at 2648.669 kb on + strand at 2648.670 kb on - strand at 2648.816 kb on + strand, within Pf6N2E2_2236 at 2648.817 kb on - strand, within Pf6N2E2_2236 at 2648.910 kb on - strand, within Pf6N2E2_2236 at 2648.910 kb on - strand, within Pf6N2E2_2236 at 2648.927 kb on + strand, within Pf6N2E2_2236 at 2648.928 kb on - strand, within Pf6N2E2_2236 at 2648.963 kb on + strand, within Pf6N2E2_2236 at 2648.963 kb on + strand, within Pf6N2E2_2236 at 2648.964 kb on - strand, within Pf6N2E2_2236 at 2649.032 kb on + strand, within Pf6N2E2_2236 at 2649.122 kb on + strand, within Pf6N2E2_2236 at 2649.122 kb on + strand, within Pf6N2E2_2236 at 2649.122 kb on + strand, within Pf6N2E2_2236 at 2649.122 kb on + strand, within Pf6N2E2_2236 at 2649.123 kb on - strand, within Pf6N2E2_2236 at 2649.136 kb on + strand, within Pf6N2E2_2236 at 2649.137 kb on - strand, within Pf6N2E2_2236 at 2649.272 kb on + strand, within Pf6N2E2_2236 at 2649.272 kb on + strand, within Pf6N2E2_2236 at 2649.272 kb on + strand, within Pf6N2E2_2236 at 2649.272 kb on + strand, within Pf6N2E2_2236 at 2649.273 kb on - strand, within Pf6N2E2_2236 at 2649.273 kb on - strand, within Pf6N2E2_2236 at 2649.384 kb on + strand, within Pf6N2E2_2236 at 2649.458 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.16 mM remove 2,646,113 - Pf6N2E2_2233 0.84 -0.0 2,646,113 - Pf6N2E2_2233 0.84 +0.1 2,646,123 + Pf6N2E2_2233 0.86 -0.7 2,646,123 + Pf6N2E2_2233 0.86 +0.8 2,646,123 + Pf6N2E2_2233 0.86 +0.7 2,646,123 + Pf6N2E2_2233 0.86 -0.1 2,646,123 + Pf6N2E2_2233 0.86 -0.3 2,646,124 - Pf6N2E2_2233 0.86 +0.3 2,646,124 - Pf6N2E2_2233 0.86 +0.8 2,646,124 - Pf6N2E2_2233 0.86 -1.2 2,646,126 + Pf6N2E2_2233 0.86 -0.2 2,646,127 - Pf6N2E2_2233 0.86 -0.4 2,646,150 + Pf6N2E2_2233 0.89 +0.7 2,646,150 + Pf6N2E2_2233 0.89 +0.7 2,646,151 - Pf6N2E2_2233 0.89 +1.2 2,646,151 - Pf6N2E2_2233 0.89 -0.7 2,646,151 - Pf6N2E2_2233 0.89 +0.5 2,646,160 + +0.2 2,646,309 + +0.5 2,646,309 + -0.1 2,646,327 + -1.5 2,646,362 - -0.5 2,646,379 + -0.8 2,646,386 + +0.6 2,646,386 + +0.1 2,646,386 + +1.6 2,646,386 + +0.1 2,646,387 - +0.1 2,646,387 - -0.0 2,646,387 - +0.1 2,646,387 - -0.3 2,646,387 - -0.0 2,646,387 - +0.5 2,646,387 - -0.1 2,646,390 + +0.7 2,646,429 + +1.2 2,646,429 + -0.6 2,646,429 + -0.4 2,646,430 - +0.1 2,646,430 - -0.4 2,646,430 - +1.8 2,646,437 - -0.1 2,646,437 - +0.1 2,646,667 + Pf6N2E2_2234 0.47 +0.4 2,646,667 + Pf6N2E2_2234 0.47 +0.9 2,646,667 + Pf6N2E2_2234 0.47 +0.1 2,646,667 + Pf6N2E2_2234 0.47 +1.0 2,646,667 + Pf6N2E2_2234 0.47 -2.4 2,646,667 + Pf6N2E2_2234 0.47 +0.9 2,646,667 + Pf6N2E2_2234 0.47 -0.4 2,646,667 + Pf6N2E2_2234 0.47 +1.4 2,646,667 + Pf6N2E2_2234 0.47 +0.7 2,646,668 - Pf6N2E2_2234 0.47 -0.4 2,646,668 - Pf6N2E2_2234 0.47 +0.3 2,646,668 - Pf6N2E2_2234 0.47 -0.6 2,646,668 - Pf6N2E2_2234 0.47 -0.1 2,646,668 - Pf6N2E2_2234 0.47 +0.3 2,646,668 - Pf6N2E2_2234 0.47 +0.2 2,646,685 + Pf6N2E2_2234 0.52 -0.6 2,646,685 + Pf6N2E2_2234 0.52 +1.0 2,646,685 + Pf6N2E2_2234 0.52 -1.4 2,646,685 + Pf6N2E2_2234 0.52 -0.3 2,646,685 + Pf6N2E2_2234 0.52 -0.7 2,646,685 + Pf6N2E2_2234 0.52 -0.6 2,646,685 + Pf6N2E2_2234 0.52 -0.0 2,646,706 + Pf6N2E2_2234 0.58 -1.7 2,646,706 + Pf6N2E2_2234 0.58 +0.3 2,646,707 - Pf6N2E2_2234 0.58 -0.7 2,646,707 - Pf6N2E2_2234 0.58 +0.1 2,646,709 + Pf6N2E2_2234 0.59 +0.2 2,646,709 + Pf6N2E2_2234 0.59 -0.1 2,646,709 + Pf6N2E2_2234 0.59 +0.6 2,646,710 - Pf6N2E2_2234 0.59 +0.6 2,646,730 + Pf6N2E2_2234 0.65 -1.1 2,646,730 + Pf6N2E2_2234 0.65 -0.2 2,646,731 - Pf6N2E2_2234 0.65 +0.5 2,646,734 - Pf6N2E2_2234 0.66 +1.7 2,646,734 - Pf6N2E2_2234 0.66 +0.4 2,646,751 + Pf6N2E2_2234 0.71 -0.1 2,646,751 + Pf6N2E2_2234 0.71 +0.5 2,646,751 + Pf6N2E2_2234 0.71 +0.6 2,646,751 + Pf6N2E2_2234 0.71 +0.1 2,646,751 + Pf6N2E2_2234 0.71 +0.0 2,646,751 + Pf6N2E2_2234 0.71 +0.6 2,646,751 + Pf6N2E2_2234 0.71 -0.7 2,646,751 + Pf6N2E2_2234 0.71 +0.6 2,646,751 + Pf6N2E2_2234 0.71 -1.0 2,646,751 + Pf6N2E2_2234 0.71 -0.2 2,646,751 + Pf6N2E2_2234 0.71 +0.6 2,646,751 + Pf6N2E2_2234 0.71 +0.7 2,646,752 - Pf6N2E2_2234 0.71 +0.5 2,646,752 - Pf6N2E2_2234 0.71 +0.6 2,646,752 - Pf6N2E2_2234 0.71 -1.6 2,646,752 - Pf6N2E2_2234 0.71 -0.1 2,646,752 - Pf6N2E2_2234 0.71 -0.6 2,646,752 - Pf6N2E2_2234 0.71 +0.2 2,646,755 + Pf6N2E2_2234 0.72 -0.3 2,646,755 + Pf6N2E2_2234 0.72 +0.1 2,646,756 - Pf6N2E2_2234 0.72 -1.2 2,646,769 + Pf6N2E2_2234 0.76 -0.0 2,646,769 + Pf6N2E2_2234 0.76 +0.2 2,646,769 + Pf6N2E2_2234 0.76 +0.0 2,646,769 + Pf6N2E2_2234 0.76 -0.5 2,646,770 - Pf6N2E2_2234 0.76 +0.5 2,646,770 - Pf6N2E2_2234 0.76 -0.4 2,646,772 + Pf6N2E2_2234 0.77 -0.2 2,646,772 + Pf6N2E2_2234 0.77 +0.6 2,646,790 + Pf6N2E2_2234 0.82 +0.2 2,646,791 - Pf6N2E2_2234 0.82 +0.2 2,646,793 + Pf6N2E2_2234 0.83 -0.4 2,646,811 + Pf6N2E2_2234 0.88 -0.1 2,646,812 - Pf6N2E2_2234 0.88 +0.4 2,646,812 - Pf6N2E2_2234 0.88 +0.5 2,646,814 + Pf6N2E2_2234 0.89 -0.1 2,646,814 + Pf6N2E2_2234 0.89 +0.3 2,646,814 + Pf6N2E2_2234 0.89 -0.5 2,646,814 + Pf6N2E2_2234 0.89 +1.0 2,646,814 + Pf6N2E2_2234 0.89 -0.2 2,646,814 + Pf6N2E2_2234 0.89 +0.7 2,646,815 - Pf6N2E2_2234 0.89 -0.9 2,646,815 - Pf6N2E2_2234 0.89 -0.4 2,646,815 - Pf6N2E2_2234 0.89 +0.1 2,646,815 - Pf6N2E2_2234 0.89 +0.7 2,646,815 - Pf6N2E2_2234 0.89 +1.2 2,646,818 - +0.0 2,647,141 + +0.1 2,647,141 + -3.0 2,647,142 - -1.6 2,647,142 - -0.7 2,647,142 - -0.7 2,647,142 - -1.5 2,647,142 - +0.5 2,647,156 - -2.1 2,647,156 - +0.4 2,647,333 + Pf6N2E2_2235 0.20 -0.1 2,647,334 - Pf6N2E2_2235 0.20 -0.4 2,647,334 - Pf6N2E2_2235 0.20 +1.7 2,647,464 + Pf6N2E2_2235 0.29 -0.4 2,647,464 + Pf6N2E2_2235 0.29 +0.4 2,647,464 + Pf6N2E2_2235 0.29 +0.9 2,647,465 - Pf6N2E2_2235 0.29 +0.7 2,647,980 + Pf6N2E2_2235 0.63 -0.6 2,647,981 - Pf6N2E2_2235 0.63 +0.4 2,648,446 + +0.0 2,648,446 + +0.7 2,648,446 + +0.6 2,648,446 + +0.1 2,648,446 + -0.8 2,648,447 - -0.9 2,648,447 - -0.5 2,648,447 - +0.4 2,648,474 - -0.2 2,648,480 + +0.6 2,648,481 - -0.7 2,648,610 + +0.2 2,648,669 + -0.1 2,648,670 - +0.2 2,648,816 + Pf6N2E2_2236 0.18 -0.9 2,648,817 - Pf6N2E2_2236 0.18 -0.1 2,648,910 - Pf6N2E2_2236 0.29 +0.0 2,648,910 - Pf6N2E2_2236 0.29 +0.7 2,648,927 + Pf6N2E2_2236 0.31 +0.6 2,648,928 - Pf6N2E2_2236 0.32 -0.3 2,648,963 + Pf6N2E2_2236 0.36 -1.0 2,648,963 + Pf6N2E2_2236 0.36 +0.2 2,648,964 - Pf6N2E2_2236 0.36 -0.6 2,649,032 + Pf6N2E2_2236 0.44 +0.1 2,649,122 + Pf6N2E2_2236 0.55 +0.4 2,649,122 + Pf6N2E2_2236 0.55 +0.1 2,649,122 + Pf6N2E2_2236 0.55 -1.6 2,649,122 + Pf6N2E2_2236 0.55 +0.3 2,649,123 - Pf6N2E2_2236 0.55 -0.5 2,649,136 + Pf6N2E2_2236 0.57 +0.1 2,649,137 - Pf6N2E2_2236 0.57 +0.8 2,649,272 + Pf6N2E2_2236 0.73 +0.4 2,649,272 + Pf6N2E2_2236 0.73 +0.0 2,649,272 + Pf6N2E2_2236 0.73 -0.5 2,649,272 + Pf6N2E2_2236 0.73 -0.2 2,649,273 - Pf6N2E2_2236 0.73 +1.0 2,649,273 - Pf6N2E2_2236 0.73 +0.0 2,649,384 + Pf6N2E2_2236 0.87 +0.4 2,649,458 + +0.4
Or see this region's nucleotide sequence