Experiment: Cobalt chloride 0.16 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1919 and Pf6N2E2_1920 are separated by 87 nucleotides Pf6N2E2_1920 and Pf6N2E2_1921 are separated by 22 nucleotides Pf6N2E2_1921 and Pf6N2E2_1922 overlap by 4 nucleotides Pf6N2E2_1922 and Pf6N2E2_1923 are separated by 284 nucleotides
Pf6N2E2_1919 - Aldehyde dehydrogenase (EC 1.2.1.3), at 2,273,106 to 2,274,578
_1919
Pf6N2E2_1920 - Rubredoxin, at 2,274,666 to 2,274,872
_1920
Pf6N2E2_1921 - hypothetical protein, at 2,274,895 to 2,275,032
_1921
Pf6N2E2_1922 - Enoyl-CoA hydratase (EC 4.2.1.17) / Delta(3)-cis-delta(2)-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35) / 3-hydroxybutyryl-CoA epimerase (EC 5.1.2.3), at 2,275,029 to 2,277,140
_1922
Pf6N2E2_1923 - Acetyl-CoA acetyltransferase, at 2,277,425 to 2,278,594
_1923
Position (kb)
2275
2276
2277
2278 Strain fitness (log2 ratio)
-2
-1
0
1 at 2274.259 kb on - strand, within Pf6N2E2_1919 at 2274.259 kb on - strand, within Pf6N2E2_1919 at 2274.341 kb on + strand, within Pf6N2E2_1919 at 2274.341 kb on + strand, within Pf6N2E2_1919 at 2274.341 kb on + strand, within Pf6N2E2_1919 at 2274.342 kb on - strand, within Pf6N2E2_1919 at 2274.344 kb on + strand, within Pf6N2E2_1919 at 2274.345 kb on - strand, within Pf6N2E2_1919 at 2274.345 kb on - strand, within Pf6N2E2_1919 at 2274.345 kb on - strand, within Pf6N2E2_1919 at 2274.557 kb on + strand at 2274.558 kb on - strand at 2274.558 kb on - strand at 2274.558 kb on - strand at 2274.664 kb on - strand at 2274.664 kb on - strand at 2274.760 kb on - strand, within Pf6N2E2_1920 at 2274.760 kb on - strand, within Pf6N2E2_1920 at 2274.777 kb on - strand, within Pf6N2E2_1920 at 2274.777 kb on - strand, within Pf6N2E2_1920 at 2274.896 kb on + strand at 2274.935 kb on + strand, within Pf6N2E2_1921 at 2275.033 kb on - strand at 2275.033 kb on - strand at 2275.033 kb on - strand at 2275.094 kb on + strand at 2275.115 kb on - strand at 2275.178 kb on - strand at 2275.216 kb on + strand at 2275.257 kb on - strand, within Pf6N2E2_1922 at 2275.257 kb on - strand, within Pf6N2E2_1922 at 2275.257 kb on - strand, within Pf6N2E2_1922 at 2275.299 kb on + strand, within Pf6N2E2_1922 at 2275.299 kb on + strand, within Pf6N2E2_1922 at 2275.335 kb on - strand, within Pf6N2E2_1922 at 2275.430 kb on - strand, within Pf6N2E2_1922 at 2275.501 kb on + strand, within Pf6N2E2_1922 at 2275.502 kb on - strand, within Pf6N2E2_1922 at 2275.541 kb on - strand, within Pf6N2E2_1922 at 2275.650 kb on + strand, within Pf6N2E2_1922 at 2275.651 kb on - strand, within Pf6N2E2_1922 at 2275.975 kb on - strand, within Pf6N2E2_1922 at 2276.026 kb on + strand, within Pf6N2E2_1922 at 2276.107 kb on + strand, within Pf6N2E2_1922 at 2276.108 kb on - strand, within Pf6N2E2_1922 at 2276.118 kb on + strand, within Pf6N2E2_1922 at 2276.119 kb on - strand, within Pf6N2E2_1922 at 2276.119 kb on - strand, within Pf6N2E2_1922 at 2276.572 kb on + strand, within Pf6N2E2_1922 at 2276.572 kb on + strand, within Pf6N2E2_1922 at 2276.631 kb on - strand, within Pf6N2E2_1922 at 2276.631 kb on - strand, within Pf6N2E2_1922 at 2276.631 kb on - strand, within Pf6N2E2_1922 at 2276.910 kb on - strand, within Pf6N2E2_1922 at 2276.942 kb on + strand at 2276.943 kb on - strand at 2277.420 kb on + strand at 2277.420 kb on + strand at 2277.432 kb on + strand at 2277.432 kb on + strand at 2277.433 kb on - strand at 2277.433 kb on - strand at 2277.446 kb on + strand at 2277.446 kb on + strand at 2277.446 kb on + strand at 2277.446 kb on + strand at 2277.446 kb on + strand at 2277.446 kb on + strand at 2277.447 kb on - strand at 2277.447 kb on - strand at 2277.447 kb on - strand at 2277.461 kb on + strand at 2277.461 kb on + strand at 2277.507 kb on + strand at 2277.507 kb on + strand at 2277.508 kb on - strand at 2277.508 kb on - strand at 2277.508 kb on - strand at 2277.606 kb on + strand, within Pf6N2E2_1923 at 2277.606 kb on + strand, within Pf6N2E2_1923 at 2277.606 kb on + strand, within Pf6N2E2_1923 at 2277.606 kb on + strand, within Pf6N2E2_1923 at 2277.607 kb on - strand, within Pf6N2E2_1923 at 2277.607 kb on - strand, within Pf6N2E2_1923 at 2277.607 kb on - strand, within Pf6N2E2_1923 at 2277.607 kb on - strand, within Pf6N2E2_1923 at 2277.623 kb on + strand, within Pf6N2E2_1923 at 2277.623 kb on + strand, within Pf6N2E2_1923 at 2277.798 kb on + strand, within Pf6N2E2_1923 at 2277.799 kb on - strand, within Pf6N2E2_1923 at 2277.839 kb on + strand, within Pf6N2E2_1923 at 2277.839 kb on + strand, within Pf6N2E2_1923 at 2277.839 kb on + strand, within Pf6N2E2_1923 at 2277.840 kb on - strand, within Pf6N2E2_1923 at 2277.840 kb on - strand, within Pf6N2E2_1923 at 2277.840 kb on - strand, within Pf6N2E2_1923 at 2277.842 kb on - strand, within Pf6N2E2_1923 at 2277.896 kb on + strand, within Pf6N2E2_1923 at 2278.002 kb on + strand, within Pf6N2E2_1923 at 2278.002 kb on + strand, within Pf6N2E2_1923 at 2278.002 kb on + strand, within Pf6N2E2_1923 at 2278.003 kb on - strand, within Pf6N2E2_1923 at 2278.079 kb on - strand, within Pf6N2E2_1923 at 2278.131 kb on + strand, within Pf6N2E2_1923
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.16 mM remove 2,274,259 - Pf6N2E2_1919 0.78 +0.4 2,274,259 - Pf6N2E2_1919 0.78 +0.1 2,274,341 + Pf6N2E2_1919 0.84 -0.0 2,274,341 + Pf6N2E2_1919 0.84 -0.7 2,274,341 + Pf6N2E2_1919 0.84 +1.0 2,274,342 - Pf6N2E2_1919 0.84 -0.9 2,274,344 + Pf6N2E2_1919 0.84 -0.1 2,274,345 - Pf6N2E2_1919 0.84 +0.1 2,274,345 - Pf6N2E2_1919 0.84 -0.1 2,274,345 - Pf6N2E2_1919 0.84 +0.8 2,274,557 + +0.5 2,274,558 - +0.7 2,274,558 - +0.1 2,274,558 - +0.5 2,274,664 - +0.7 2,274,664 - +0.1 2,274,760 - Pf6N2E2_1920 0.45 +0.4 2,274,760 - Pf6N2E2_1920 0.45 +0.8 2,274,777 - Pf6N2E2_1920 0.54 +0.4 2,274,777 - Pf6N2E2_1920 0.54 -0.1 2,274,896 + +0.1 2,274,935 + Pf6N2E2_1921 0.29 -1.5 2,275,033 - +0.5 2,275,033 - -0.6 2,275,033 - +0.5 2,275,094 + -2.0 2,275,115 - +0.2 2,275,178 - +0.1 2,275,216 + -0.2 2,275,257 - Pf6N2E2_1922 0.11 +0.1 2,275,257 - Pf6N2E2_1922 0.11 +0.6 2,275,257 - Pf6N2E2_1922 0.11 -0.7 2,275,299 + Pf6N2E2_1922 0.13 +0.5 2,275,299 + Pf6N2E2_1922 0.13 +0.4 2,275,335 - Pf6N2E2_1922 0.14 -0.3 2,275,430 - Pf6N2E2_1922 0.19 -0.3 2,275,501 + Pf6N2E2_1922 0.22 +0.7 2,275,502 - Pf6N2E2_1922 0.22 -1.0 2,275,541 - Pf6N2E2_1922 0.24 -0.0 2,275,650 + Pf6N2E2_1922 0.29 +0.6 2,275,651 - Pf6N2E2_1922 0.29 +0.0 2,275,975 - Pf6N2E2_1922 0.45 +0.2 2,276,026 + Pf6N2E2_1922 0.47 +0.3 2,276,107 + Pf6N2E2_1922 0.51 +0.6 2,276,108 - Pf6N2E2_1922 0.51 +0.2 2,276,118 + Pf6N2E2_1922 0.52 -0.7 2,276,119 - Pf6N2E2_1922 0.52 +0.4 2,276,119 - Pf6N2E2_1922 0.52 +0.5 2,276,572 + Pf6N2E2_1922 0.73 +0.4 2,276,572 + Pf6N2E2_1922 0.73 +0.6 2,276,631 - Pf6N2E2_1922 0.76 +0.0 2,276,631 - Pf6N2E2_1922 0.76 -0.1 2,276,631 - Pf6N2E2_1922 0.76 +1.5 2,276,910 - Pf6N2E2_1922 0.89 +0.4 2,276,942 + -0.2 2,276,943 - -0.6 2,277,420 + +0.2 2,277,420 + +0.2 2,277,432 + -0.5 2,277,432 + +0.6 2,277,433 - +0.7 2,277,433 - -0.8 2,277,446 + +0.2 2,277,446 + -0.1 2,277,446 + -0.1 2,277,446 + +0.3 2,277,446 + -0.2 2,277,446 + -0.0 2,277,447 - +1.1 2,277,447 - +0.3 2,277,447 - -0.6 2,277,461 + +1.4 2,277,461 + +0.7 2,277,507 + +0.1 2,277,507 + -0.6 2,277,508 - +0.2 2,277,508 - -1.6 2,277,508 - -0.7 2,277,606 + Pf6N2E2_1923 0.15 +0.4 2,277,606 + Pf6N2E2_1923 0.15 -0.0 2,277,606 + Pf6N2E2_1923 0.15 -0.5 2,277,606 + Pf6N2E2_1923 0.15 +0.3 2,277,607 - Pf6N2E2_1923 0.16 -0.6 2,277,607 - Pf6N2E2_1923 0.16 +0.6 2,277,607 - Pf6N2E2_1923 0.16 +1.3 2,277,607 - Pf6N2E2_1923 0.16 +0.3 2,277,623 + Pf6N2E2_1923 0.17 -0.1 2,277,623 + Pf6N2E2_1923 0.17 -1.4 2,277,798 + Pf6N2E2_1923 0.32 +1.3 2,277,799 - Pf6N2E2_1923 0.32 -0.2 2,277,839 + Pf6N2E2_1923 0.35 +0.8 2,277,839 + Pf6N2E2_1923 0.35 +0.9 2,277,839 + Pf6N2E2_1923 0.35 +0.9 2,277,840 - Pf6N2E2_1923 0.35 -0.2 2,277,840 - Pf6N2E2_1923 0.35 +0.2 2,277,840 - Pf6N2E2_1923 0.35 -0.0 2,277,842 - Pf6N2E2_1923 0.36 -0.0 2,277,896 + Pf6N2E2_1923 0.40 -0.0 2,278,002 + Pf6N2E2_1923 0.49 +1.1 2,278,002 + Pf6N2E2_1923 0.49 +0.8 2,278,002 + Pf6N2E2_1923 0.49 +1.4 2,278,003 - Pf6N2E2_1923 0.49 -2.5 2,278,079 - Pf6N2E2_1923 0.56 -0.2 2,278,131 + Pf6N2E2_1923 0.60 +1.1
Or see this region's nucleotide sequence