Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS07985 and MMJJ_RS07990 are separated by 30 nucleotides MMJJ_RS07990 and MMJJ_RS07995 are separated by 37 nucleotides MMJJ_RS07995 and MMJJ_RS08000 overlap by 8 nucleotides MMJJ_RS08000 and MMJJ_RS08005 are separated by 79 nucleotides
MMJJ_RS07985: MMJJ_RS07985 - hypothetical protein, at 1,464,225 to 1,464,425
_RS07985
MMJJ_RS07990: MMJJ_RS07990 - UPF0280 family protein, at 1,464,456 to 1,465,199
_RS07990
MMJJ_RS07995: MMJJ_RS07995 - molybdenum cofactor biosynthesis protein MoaE, at 1,465,237 to 1,465,662
_RS07995
MMJJ_RS08000: MMJJ_RS08000 - HesA/MoeB/ThiF family protein, at 1,465,655 to 1,466,374
_RS08000
MMJJ_RS08005: MMJJ_RS08005 - formate dehydrogenase accessory sulfurtransferase FdhD, at 1,466,454 to 1,467,155
_RS08005
Position (kb)
1465
1466 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2 at 1464.267 kb on + strand, within MMJJ_RS07985 at 1464.295 kb on - strand, within MMJJ_RS07985 at 1464.295 kb on - strand, within MMJJ_RS07985 at 1464.296 kb on + strand, within MMJJ_RS07985 at 1464.296 kb on + strand, within MMJJ_RS07985 at 1464.307 kb on + strand, within MMJJ_RS07985 at 1464.307 kb on + strand, within MMJJ_RS07985 at 1464.346 kb on - strand, within MMJJ_RS07985 at 1464.346 kb on - strand, within MMJJ_RS07985 at 1464.347 kb on + strand, within MMJJ_RS07985 at 1464.369 kb on - strand, within MMJJ_RS07985 at 1464.370 kb on + strand, within MMJJ_RS07985 at 1464.379 kb on + strand, within MMJJ_RS07985 at 1464.379 kb on + strand, within MMJJ_RS07985 at 1464.399 kb on - strand, within MMJJ_RS07985 at 1464.401 kb on - strand, within MMJJ_RS07985 at 1464.431 kb on + strand at 1464.439 kb on - strand at 1464.439 kb on - strand at 1464.439 kb on - strand at 1464.439 kb on - strand at 1464.440 kb on + strand at 1464.440 kb on + strand at 1464.441 kb on - strand at 1464.446 kb on - strand at 1464.447 kb on + strand at 1464.453 kb on + strand at 1464.453 kb on + strand at 1464.453 kb on + strand at 1464.550 kb on + strand, within MMJJ_RS07990 at 1465.047 kb on + strand, within MMJJ_RS07990 at 1465.139 kb on - strand at 1465.213 kb on + strand at 1465.224 kb on + strand at 1465.228 kb on - strand at 1465.239 kb on + strand at 1465.239 kb on + strand at 1465.249 kb on - strand at 1465.307 kb on + strand, within MMJJ_RS07995 at 1465.369 kb on - strand, within MMJJ_RS07995 at 1465.370 kb on + strand, within MMJJ_RS07995 at 1465.375 kb on - strand, within MMJJ_RS07995 at 1465.380 kb on - strand, within MMJJ_RS07995 at 1465.380 kb on - strand, within MMJJ_RS07995 at 1465.381 kb on + strand, within MMJJ_RS07995 at 1465.431 kb on - strand, within MMJJ_RS07995 at 1465.549 kb on - strand, within MMJJ_RS07995 at 1465.549 kb on - strand, within MMJJ_RS07995 at 1465.588 kb on - strand, within MMJJ_RS07995 at 1465.588 kb on - strand, within MMJJ_RS07995 at 1465.588 kb on - strand, within MMJJ_RS07995 at 1465.589 kb on + strand, within MMJJ_RS07995 at 1465.636 kb on + strand at 1465.665 kb on + strand at 1465.700 kb on - strand at 1465.701 kb on + strand at 1465.732 kb on - strand, within MMJJ_RS08000 at 1465.733 kb on + strand, within MMJJ_RS08000 at 1465.733 kb on + strand, within MMJJ_RS08000 at 1465.737 kb on + strand, within MMJJ_RS08000 at 1465.751 kb on + strand, within MMJJ_RS08000 at 1465.778 kb on - strand, within MMJJ_RS08000 at 1465.781 kb on - strand, within MMJJ_RS08000 at 1465.852 kb on - strand, within MMJJ_RS08000 at 1465.872 kb on + strand, within MMJJ_RS08000 at 1465.882 kb on - strand, within MMJJ_RS08000 at 1465.882 kb on - strand, within MMJJ_RS08000 at 1465.891 kb on - strand, within MMJJ_RS08000 at 1465.892 kb on + strand, within MMJJ_RS08000 at 1465.922 kb on + strand, within MMJJ_RS08000 at 1465.934 kb on - strand, within MMJJ_RS08000 at 1465.934 kb on - strand, within MMJJ_RS08000 at 1465.969 kb on - strand, within MMJJ_RS08000 at 1465.970 kb on + strand, within MMJJ_RS08000 at 1465.970 kb on + strand, within MMJJ_RS08000 at 1465.971 kb on - strand, within MMJJ_RS08000 at 1465.972 kb on + strand, within MMJJ_RS08000 at 1465.988 kb on + strand, within MMJJ_RS08000 at 1465.994 kb on - strand, within MMJJ_RS08000 at 1465.994 kb on - strand, within MMJJ_RS08000 at 1465.995 kb on + strand at 1465.998 kb on - strand, within MMJJ_RS08000 at 1465.999 kb on + strand, within MMJJ_RS08000 at 1465.999 kb on + strand, within MMJJ_RS08000 at 1466.003 kb on + strand, within MMJJ_RS08000 at 1466.009 kb on + strand, within MMJJ_RS08000 at 1466.014 kb on - strand, within MMJJ_RS08000 at 1466.033 kb on + strand, within MMJJ_RS08000 at 1466.090 kb on - strand, within MMJJ_RS08000 at 1466.091 kb on + strand, within MMJJ_RS08000 at 1466.102 kb on + strand, within MMJJ_RS08000 at 1466.105 kb on + strand, within MMJJ_RS08000 at 1466.132 kb on + strand, within MMJJ_RS08000 at 1466.181 kb on + strand, within MMJJ_RS08000 at 1466.244 kb on + strand, within MMJJ_RS08000 at 1466.291 kb on - strand, within MMJJ_RS08000 at 1466.342 kb on - strand at 1466.358 kb on - strand at 1466.359 kb on + strand at 1466.359 kb on + strand at 1466.375 kb on + strand at 1466.375 kb on + strand at 1466.377 kb on - strand at 1466.386 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.398 kb on - strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.399 kb on + strand at 1466.400 kb on - strand at 1466.407 kb on - strand at 1466.407 kb on - strand at 1466.407 kb on - strand at 1466.407 kb on - strand at 1466.413 kb on + strand at 1466.414 kb on - strand at 1466.414 kb on - strand at 1466.414 kb on - strand at 1466.415 kb on + strand at 1466.415 kb on + strand at 1466.418 kb on + strand at 1466.418 kb on + strand at 1466.420 kb on - strand at 1466.420 kb on - strand at 1466.420 kb on - strand at 1466.429 kb on - strand at 1466.429 kb on - strand at 1466.430 kb on + strand at 1466.430 kb on + strand at 1466.460 kb on + strand at 1466.478 kb on + strand at 1466.484 kb on - strand at 1466.534 kb on + strand, within MMJJ_RS08005 at 1466.557 kb on + strand, within MMJJ_RS08005 at 1466.573 kb on + strand, within MMJJ_RS08005 at 1466.590 kb on - strand, within MMJJ_RS08005 at 1466.644 kb on + strand, within MMJJ_RS08005
Per-strain Table
Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE remove 1,464,267 + MMJJ_RS07985 0.21 +2.1 1,464,295 - MMJJ_RS07985 0.35 -0.4 1,464,295 - MMJJ_RS07985 0.35 -0.7 1,464,296 + MMJJ_RS07985 0.35 -1.2 1,464,296 + MMJJ_RS07985 0.35 +0.5 1,464,307 + MMJJ_RS07985 0.41 +0.2 1,464,307 + MMJJ_RS07985 0.41 +0.8 1,464,346 - MMJJ_RS07985 0.60 -0.2 1,464,346 - MMJJ_RS07985 0.60 +0.8 1,464,347 + MMJJ_RS07985 0.61 -0.5 1,464,369 - MMJJ_RS07985 0.72 +2.0 1,464,370 + MMJJ_RS07985 0.72 +0.4 1,464,379 + MMJJ_RS07985 0.77 -0.6 1,464,379 + MMJJ_RS07985 0.77 -0.0 1,464,399 - MMJJ_RS07985 0.87 +1.9 1,464,401 - MMJJ_RS07985 0.88 -0.2 1,464,431 + -0.1 1,464,439 - -0.0 1,464,439 - -0.0 1,464,439 - -0.0 1,464,439 - +2.0 1,464,440 + +0.8 1,464,440 + +0.6 1,464,441 - +1.0 1,464,446 - -0.8 1,464,447 + +0.8 1,464,453 + -0.3 1,464,453 + +0.4 1,464,453 + -0.9 1,464,550 + MMJJ_RS07990 0.13 +1.0 1,465,047 + MMJJ_RS07990 0.79 -1.0 1,465,139 - -1.6 1,465,213 + -2.3 1,465,224 + -3.5 1,465,228 - +0.6 1,465,239 + -1.6 1,465,239 + -1.6 1,465,249 - -0.5 1,465,307 + MMJJ_RS07995 0.16 -4.0 1,465,369 - MMJJ_RS07995 0.31 -4.6 1,465,370 + MMJJ_RS07995 0.31 -2.8 1,465,375 - MMJJ_RS07995 0.32 -0.0 1,465,380 - MMJJ_RS07995 0.34 -4.6 1,465,380 - MMJJ_RS07995 0.34 -4.3 1,465,381 + MMJJ_RS07995 0.34 -4.4 1,465,431 - MMJJ_RS07995 0.46 -4.9 1,465,549 - MMJJ_RS07995 0.73 -3.8 1,465,549 - MMJJ_RS07995 0.73 -4.7 1,465,588 - MMJJ_RS07995 0.82 -3.5 1,465,588 - MMJJ_RS07995 0.82 -2.0 1,465,588 - MMJJ_RS07995 0.82 -2.0 1,465,589 + MMJJ_RS07995 0.83 -1.5 1,465,636 + -4.9 1,465,665 + -1.6 1,465,700 - -5.9 1,465,701 + -4.4 1,465,732 - MMJJ_RS08000 0.11 -2.0 1,465,733 + MMJJ_RS08000 0.11 -4.8 1,465,733 + MMJJ_RS08000 0.11 -3.5 1,465,737 + MMJJ_RS08000 0.11 +0.1 1,465,751 + MMJJ_RS08000 0.13 -4.7 1,465,778 - MMJJ_RS08000 0.17 -3.8 1,465,781 - MMJJ_RS08000 0.17 -1.6 1,465,852 - MMJJ_RS08000 0.27 -3.9 1,465,872 + MMJJ_RS08000 0.30 -4.0 1,465,882 - MMJJ_RS08000 0.32 -3.4 1,465,882 - MMJJ_RS08000 0.32 -2.2 1,465,891 - MMJJ_RS08000 0.33 -4.3 1,465,892 + MMJJ_RS08000 0.33 -3.7 1,465,922 + MMJJ_RS08000 0.37 -4.8 1,465,934 - MMJJ_RS08000 0.39 -2.0 1,465,934 - MMJJ_RS08000 0.39 -1.6 1,465,969 - MMJJ_RS08000 0.44 -2.4 1,465,970 + MMJJ_RS08000 0.44 -1.6 1,465,970 + MMJJ_RS08000 0.44 -2.0 1,465,971 - MMJJ_RS08000 0.44 -0.0 1,465,972 + MMJJ_RS08000 0.44 -3.8 1,465,988 + MMJJ_RS08000 0.46 +0.8 1,465,994 - MMJJ_RS08000 0.47 -3.4 1,465,994 - MMJJ_RS08000 0.47 -5.1 1,465,995 + -0.0 1,465,998 - MMJJ_RS08000 0.48 -4.3 1,465,999 + MMJJ_RS08000 0.48 -3.9 1,465,999 + MMJJ_RS08000 0.48 -2.6 1,466,003 + MMJJ_RS08000 0.48 -4.6 1,466,009 + MMJJ_RS08000 0.49 -3.7 1,466,014 - MMJJ_RS08000 0.50 -2.7 1,466,033 + MMJJ_RS08000 0.53 -2.0 1,466,090 - MMJJ_RS08000 0.60 -4.1 1,466,091 + MMJJ_RS08000 0.61 -0.6 1,466,102 + MMJJ_RS08000 0.62 -5.0 1,466,105 + MMJJ_RS08000 0.62 -3.8 1,466,132 + MMJJ_RS08000 0.66 -3.9 1,466,181 + MMJJ_RS08000 0.73 -2.9 1,466,244 + MMJJ_RS08000 0.82 -2.4 1,466,291 - MMJJ_RS08000 0.88 -4.0 1,466,342 - -3.5 1,466,358 - -2.3 1,466,359 + -4.4 1,466,359 + -2.6 1,466,375 + -4.0 1,466,375 + -5.0 1,466,377 - -2.8 1,466,386 - -1.4 1,466,398 - -0.3 1,466,398 - -0.2 1,466,398 - -1.8 1,466,398 - +0.6 1,466,398 - -0.4 1,466,398 - +1.1 1,466,398 - +0.3 1,466,398 - -0.7 1,466,398 - -1.0 1,466,399 + -0.1 1,466,399 + +0.1 1,466,399 + -0.4 1,466,399 + -1.5 1,466,399 + -0.8 1,466,399 + -0.7 1,466,399 + -0.6 1,466,399 + -0.8 1,466,399 + +0.1 1,466,400 - -0.7 1,466,407 - +1.8 1,466,407 - -0.5 1,466,407 - +0.2 1,466,407 - +2.6 1,466,413 + +0.1 1,466,414 - -1.0 1,466,414 - +0.5 1,466,414 - +1.6 1,466,415 + +0.6 1,466,415 + +0.0 1,466,418 + -0.2 1,466,418 + -0.8 1,466,420 - -0.0 1,466,420 - -2.4 1,466,420 - -0.2 1,466,429 - +0.1 1,466,429 - +1.3 1,466,430 + +0.2 1,466,430 + -0.4 1,466,460 + -3.9 1,466,478 + -3.2 1,466,484 - -0.0 1,466,534 + MMJJ_RS08005 0.11 -4.6 1,466,557 + MMJJ_RS08005 0.15 -4.6 1,466,573 + MMJJ_RS08005 0.17 -2.4 1,466,590 - MMJJ_RS08005 0.19 -3.7 1,466,644 + MMJJ_RS08005 0.27 -3.3
Or see this region's nucleotide sequence