Experiment: R2A with Manganese (II) chloride tetrahydrate 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS02525 and LRK54_RS02530 are separated by 113 nucleotides LRK54_RS02530 and LRK54_RS02535 are separated by 289 nucleotides LRK54_RS02535 and LRK54_RS02540 are separated by 151 nucleotides
LRK54_RS02525: LRK54_RS02525 - DNA repair protein RadA, at 546,037 to 547,422
_RS02525
LRK54_RS02530: LRK54_RS02530 - replicative DNA helicase, at 547,536 to 548,930
_RS02530
LRK54_RS02535: LRK54_RS02535 - 50S ribosomal protein L9, at 549,220 to 549,669
_RS02535
LRK54_RS02540: LRK54_RS02540 - 30S ribosomal protein S18, at 549,821 to 550,051
_RS02540
Position (kb) 547
548
549 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 546.692 kb on + strand, within LRK54_RS02525 at 546.692 kb on + strand, within LRK54_RS02525 at 546.692 kb on + strand, within LRK54_RS02525 at 546.692 kb on + strand, within LRK54_RS02525 at 546.692 kb on + strand, within LRK54_RS02525 at 546.692 kb on + strand, within LRK54_RS02525 at 546.692 kb on + strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.693 kb on - strand, within LRK54_RS02525 at 546.797 kb on + strand, within LRK54_RS02525 at 546.797 kb on + strand, within LRK54_RS02525 at 546.797 kb on + strand, within LRK54_RS02525 at 546.797 kb on + strand, within LRK54_RS02525 at 546.797 kb on + strand, within LRK54_RS02525 at 546.798 kb on - strand, within LRK54_RS02525 at 546.798 kb on - strand, within LRK54_RS02525 at 546.863 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.037 kb on + strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.038 kb on - strand, within LRK54_RS02525 at 547.259 kb on + strand, within LRK54_RS02525 at 547.259 kb on + strand, within LRK54_RS02525 at 547.259 kb on + strand, within LRK54_RS02525 at 547.259 kb on + strand, within LRK54_RS02525 at 547.259 kb on + strand, within LRK54_RS02525 at 547.260 kb on - strand, within LRK54_RS02525 at 547.260 kb on - strand, within LRK54_RS02525 at 547.260 kb on - strand, within LRK54_RS02525 at 547.260 kb on - strand, within LRK54_RS02525 at 547.260 kb on - strand, within LRK54_RS02525 at 547.260 kb on - strand, within LRK54_RS02525 at 547.260 kb on - strand, within LRK54_RS02525 at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.400 kb on + strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.401 kb on - strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.489 kb on + strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.490 kb on - strand at 547.570 kb on + strand at 547.570 kb on + strand at 547.570 kb on + strand at 547.570 kb on + strand at 547.570 kb on + strand at 547.570 kb on + strand at 547.570 kb on + strand at 547.570 kb on + strand at 547.571 kb on - strand at 547.571 kb on - strand at 547.571 kb on - strand at 547.571 kb on - strand at 547.571 kb on - strand at 547.571 kb on - strand at 547.571 kb on - strand at 547.571 kb on - strand at 548.937 kb on - strand at 548.937 kb on - strand at 548.937 kb on - strand at 548.950 kb on - strand at 549.034 kb on + strand at 549.034 kb on + strand at 549.034 kb on + strand at 549.035 kb on - strand at 549.035 kb on - strand at 549.035 kb on - strand at 549.035 kb on - strand at 549.035 kb on - strand at 549.035 kb on - strand at 549.035 kb on - strand at 549.035 kb on - strand at 549.035 kb on - strand at 549.035 kb on - strand at 549.051 kb on + strand at 549.051 kb on + strand at 549.052 kb on - strand at 549.072 kb on + strand at 549.072 kb on + strand at 549.072 kb on + strand at 549.072 kb on + strand at 549.072 kb on + strand at 549.072 kb on + strand at 549.073 kb on - strand at 549.073 kb on - strand at 549.073 kb on - strand at 549.073 kb on - strand at 549.073 kb on - strand at 549.148 kb on + strand at 549.148 kb on + strand at 549.148 kb on + strand at 549.149 kb on - strand at 549.149 kb on - strand at 549.149 kb on - strand at 549.214 kb on + strand at 549.214 kb on + strand at 549.214 kb on + strand at 549.215 kb on - strand at 549.215 kb on - strand at 549.215 kb on - strand at 549.215 kb on - strand at 549.215 kb on - strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.221 kb on + strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.222 kb on - strand at 549.290 kb on + strand, within LRK54_RS02535 at 549.290 kb on + strand, within LRK54_RS02535 at 549.290 kb on + strand, within LRK54_RS02535 at 549.290 kb on + strand, within LRK54_RS02535 at 549.290 kb on + strand, within LRK54_RS02535 at 549.290 kb on + strand, within LRK54_RS02535 at 549.291 kb on - strand at 549.291 kb on - strand, within LRK54_RS02535 at 549.291 kb on - strand, within LRK54_RS02535 at 549.291 kb on - strand, within LRK54_RS02535 at 549.327 kb on + strand, within LRK54_RS02535 at 549.328 kb on - strand, within LRK54_RS02535 at 549.328 kb on - strand, within LRK54_RS02535 at 549.328 kb on - strand, within LRK54_RS02535 at 549.328 kb on - strand, within LRK54_RS02535 at 549.509 kb on + strand, within LRK54_RS02535 at 549.509 kb on + strand, within LRK54_RS02535 at 549.509 kb on + strand, within LRK54_RS02535 at 549.509 kb on + strand, within LRK54_RS02535 at 549.510 kb on - strand, within LRK54_RS02535 at 549.510 kb on - strand, within LRK54_RS02535 at 549.510 kb on - strand, within LRK54_RS02535 at 549.510 kb on - strand, within LRK54_RS02535 at 549.510 kb on - strand, within LRK54_RS02535 at 549.510 kb on - strand, within LRK54_RS02535 at 549.584 kb on + strand, within LRK54_RS02535 at 549.584 kb on + strand, within LRK54_RS02535 at 549.584 kb on + strand, within LRK54_RS02535 at 549.584 kb on + strand, within LRK54_RS02535 at 549.584 kb on + strand, within LRK54_RS02535 at 549.584 kb on + strand, within LRK54_RS02535 at 549.584 kb on + strand, within LRK54_RS02535 at 549.584 kb on + strand, within LRK54_RS02535 at 549.584 kb on + strand, within LRK54_RS02535 at 549.584 kb on + strand, within LRK54_RS02535 at 549.584 kb on + strand, within LRK54_RS02535 at 549.584 kb on + strand, within LRK54_RS02535 at 549.585 kb on - strand, within LRK54_RS02535 at 549.585 kb on - strand, within LRK54_RS02535 at 549.585 kb on - strand, within LRK54_RS02535 at 549.585 kb on - strand, within LRK54_RS02535 at 549.585 kb on - strand, within LRK54_RS02535 at 549.585 kb on - strand, within LRK54_RS02535 at 549.585 kb on - strand, within LRK54_RS02535 at 549.585 kb on - strand, within LRK54_RS02535 at 549.631 kb on - strand at 549.790 kb on + strand at 549.790 kb on + strand at 549.804 kb on + strand at 549.804 kb on + strand at 549.804 kb on + strand at 549.844 kb on - strand, within LRK54_RS02540
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Manganese (II) chloride tetrahydrate 2.5 mM remove 546,692 + LRK54_RS02525 0.47 -0.3 546,692 + LRK54_RS02525 0.47 +0.7 546,692 + LRK54_RS02525 0.47 -0.6 546,692 + LRK54_RS02525 0.47 +0.7 546,692 + LRK54_RS02525 0.47 -1.6 546,692 + LRK54_RS02525 0.47 +0.5 546,692 + LRK54_RS02525 0.47 +0.9 546,693 - LRK54_RS02525 0.47 +1.4 546,693 - LRK54_RS02525 0.47 -0.5 546,693 - LRK54_RS02525 0.47 +1.1 546,693 - LRK54_RS02525 0.47 +0.7 546,693 - LRK54_RS02525 0.47 -0.4 546,693 - LRK54_RS02525 0.47 +2.2 546,693 - LRK54_RS02525 0.47 +0.4 546,693 - LRK54_RS02525 0.47 -0.9 546,693 - LRK54_RS02525 0.47 -0.1 546,797 + LRK54_RS02525 0.55 -0.6 546,797 + LRK54_RS02525 0.55 +1.1 546,797 + LRK54_RS02525 0.55 +0.5 546,797 + LRK54_RS02525 0.55 -2.8 546,797 + LRK54_RS02525 0.55 +0.8 546,798 - LRK54_RS02525 0.55 -1.2 546,798 - LRK54_RS02525 0.55 +0.1 546,863 + LRK54_RS02525 0.60 -0.4 547,037 + LRK54_RS02525 0.72 -0.5 547,037 + LRK54_RS02525 0.72 -0.7 547,037 + LRK54_RS02525 0.72 -1.0 547,037 + LRK54_RS02525 0.72 -0.5 547,037 + LRK54_RS02525 0.72 +0.9 547,037 + LRK54_RS02525 0.72 -1.6 547,037 + LRK54_RS02525 0.72 -0.4 547,037 + LRK54_RS02525 0.72 -0.2 547,037 + LRK54_RS02525 0.72 +0.8 547,037 + LRK54_RS02525 0.72 -1.9 547,037 + LRK54_RS02525 0.72 -0.9 547,037 + LRK54_RS02525 0.72 +0.1 547,037 + LRK54_RS02525 0.72 -1.4 547,037 + LRK54_RS02525 0.72 -0.2 547,037 + LRK54_RS02525 0.72 +0.6 547,037 + LRK54_RS02525 0.72 -0.2 547,037 + LRK54_RS02525 0.72 -0.4 547,037 + LRK54_RS02525 0.72 +0.3 547,037 + LRK54_RS02525 0.72 -0.4 547,037 + LRK54_RS02525 0.72 -1.1 547,037 + LRK54_RS02525 0.72 -0.4 547,037 + LRK54_RS02525 0.72 -0.3 547,038 - LRK54_RS02525 0.72 +0.1 547,038 - LRK54_RS02525 0.72 -0.6 547,038 - LRK54_RS02525 0.72 +0.1 547,038 - LRK54_RS02525 0.72 -1.5 547,038 - LRK54_RS02525 0.72 -0.6 547,038 - LRK54_RS02525 0.72 -1.3 547,038 - LRK54_RS02525 0.72 +0.2 547,038 - LRK54_RS02525 0.72 -0.1 547,038 - LRK54_RS02525 0.72 -0.7 547,038 - LRK54_RS02525 0.72 -0.0 547,038 - LRK54_RS02525 0.72 +0.3 547,038 - LRK54_RS02525 0.72 +1.1 547,038 - LRK54_RS02525 0.72 -0.2 547,038 - LRK54_RS02525 0.72 +0.3 547,038 - LRK54_RS02525 0.72 -1.5 547,038 - LRK54_RS02525 0.72 +1.4 547,038 - LRK54_RS02525 0.72 +0.4 547,038 - LRK54_RS02525 0.72 -0.6 547,038 - LRK54_RS02525 0.72 +0.1 547,038 - LRK54_RS02525 0.72 +0.7 547,259 + LRK54_RS02525 0.88 -0.2 547,259 + LRK54_RS02525 0.88 -2.3 547,259 + LRK54_RS02525 0.88 -0.2 547,259 + LRK54_RS02525 0.88 +0.6 547,259 + LRK54_RS02525 0.88 -0.3 547,260 - LRK54_RS02525 0.88 +0.6 547,260 - LRK54_RS02525 0.88 +0.4 547,260 - LRK54_RS02525 0.88 -2.4 547,260 - LRK54_RS02525 0.88 -0.8 547,260 - LRK54_RS02525 0.88 -0.2 547,260 - LRK54_RS02525 0.88 -0.0 547,260 - LRK54_RS02525 0.88 -1.2 547,400 + -0.1 547,400 + -0.5 547,400 + -0.8 547,400 + +0.0 547,400 + -0.4 547,400 + -1.1 547,400 + +1.3 547,400 + -0.0 547,400 + -0.2 547,400 + +0.0 547,400 + -1.1 547,400 + -0.8 547,400 + -0.7 547,400 + -0.3 547,401 - -0.8 547,401 - +0.9 547,401 - -1.5 547,401 - +0.4 547,401 - -0.4 547,401 - -0.6 547,401 - -0.4 547,401 - -0.7 547,401 - +0.2 547,489 + +2.5 547,489 + +0.2 547,489 + +0.5 547,489 + +0.5 547,489 + -0.3 547,489 + +0.1 547,489 + -0.3 547,489 + +0.5 547,489 + +0.3 547,490 - +2.1 547,490 - +0.1 547,490 - -0.4 547,490 - -0.6 547,490 - -1.1 547,490 - +0.1 547,490 - +0.2 547,490 - +0.6 547,490 - +0.6 547,490 - -0.6 547,490 - -0.7 547,490 - -0.6 547,490 - +0.1 547,490 - -0.5 547,490 - -0.5 547,490 - -1.1 547,570 + -0.2 547,570 + -0.1 547,570 + -0.3 547,570 + -0.4 547,570 + +0.3 547,570 + -0.3 547,570 + -1.1 547,570 + -0.3 547,571 - -1.1 547,571 - -0.7 547,571 - -0.3 547,571 - +0.9 547,571 - -0.8 547,571 - +0.2 547,571 - +0.6 547,571 - -0.6 548,937 - +0.1 548,937 - -1.1 548,937 - +1.4 548,950 - -1.0 549,034 + +1.4 549,034 + -0.1 549,034 + -0.7 549,035 - -1.1 549,035 - -1.9 549,035 - -0.2 549,035 - -1.3 549,035 - +0.4 549,035 - +0.1 549,035 - -0.7 549,035 - +0.2 549,035 - -0.2 549,035 - +0.5 549,051 + +0.6 549,051 + +0.3 549,052 - -0.8 549,072 + +0.1 549,072 + -0.1 549,072 + +0.3 549,072 + -0.6 549,072 + -2.4 549,072 + -2.1 549,073 - -0.7 549,073 - -0.2 549,073 - +0.7 549,073 - -0.5 549,073 - -0.5 549,148 + +0.3 549,148 + -0.7 549,148 + -1.5 549,149 - -0.2 549,149 - -0.4 549,149 - +0.7 549,214 + -0.6 549,214 + +1.3 549,214 + +0.2 549,215 - +1.6 549,215 - +0.8 549,215 - +1.7 549,215 - -0.3 549,215 - +0.3 549,221 + +1.1 549,221 + +0.9 549,221 + +0.3 549,221 + -0.1 549,221 + +1.7 549,221 + +0.9 549,221 + +0.1 549,221 + +0.2 549,221 + +0.5 549,221 + +0.2 549,221 + +0.1 549,221 + +0.7 549,221 + +0.1 549,221 + -1.3 549,221 + +0.3 549,221 + +0.6 549,221 + +0.9 549,221 + +0.9 549,221 + +0.6 549,221 + +0.1 549,221 + +0.6 549,221 + +0.4 549,222 - +0.4 549,222 - +0.6 549,222 - +0.4 549,222 - +0.2 549,222 - +1.0 549,222 - -0.1 549,222 - -0.8 549,222 - +1.1 549,222 - -0.8 549,222 - +0.1 549,222 - +0.3 549,222 - +1.2 549,222 - +0.4 549,222 - +0.9 549,222 - -1.2 549,222 - -0.7 549,222 - +0.8 549,222 - -0.3 549,222 - +0.4 549,222 - -0.9 549,222 - +0.1 549,222 - -0.8 549,222 - -1.0 549,222 - -0.6 549,222 - +0.1 549,222 - +0.8 549,222 - -0.2 549,222 - +0.4 549,222 - +0.4 549,222 - +1.0 549,222 - +0.5 549,222 - -1.0 549,290 + LRK54_RS02535 0.16 +0.1 549,290 + LRK54_RS02535 0.16 +1.5 549,290 + LRK54_RS02535 0.16 -0.5 549,290 + LRK54_RS02535 0.16 +0.1 549,290 + LRK54_RS02535 0.16 -0.4 549,290 + LRK54_RS02535 0.16 -0.0 549,291 - +3.0 549,291 - LRK54_RS02535 0.16 -0.7 549,291 - LRK54_RS02535 0.16 +0.6 549,291 - LRK54_RS02535 0.16 -0.5 549,327 + LRK54_RS02535 0.24 -1.2 549,328 - LRK54_RS02535 0.24 -0.3 549,328 - LRK54_RS02535 0.24 +0.5 549,328 - LRK54_RS02535 0.24 -1.7 549,328 - LRK54_RS02535 0.24 +1.0 549,509 + LRK54_RS02535 0.64 -0.5 549,509 + LRK54_RS02535 0.64 +1.1 549,509 + LRK54_RS02535 0.64 -0.9 549,509 + LRK54_RS02535 0.64 -0.1 549,510 - LRK54_RS02535 0.64 -0.6 549,510 - LRK54_RS02535 0.64 -0.8 549,510 - LRK54_RS02535 0.64 -0.4 549,510 - LRK54_RS02535 0.64 +0.6 549,510 - LRK54_RS02535 0.64 +1.0 549,510 - LRK54_RS02535 0.64 -0.3 549,584 + LRK54_RS02535 0.81 +0.9 549,584 + LRK54_RS02535 0.81 -0.4 549,584 + LRK54_RS02535 0.81 +1.3 549,584 + LRK54_RS02535 0.81 -0.4 549,584 + LRK54_RS02535 0.81 -0.1 549,584 + LRK54_RS02535 0.81 -0.2 549,584 + LRK54_RS02535 0.81 -0.2 549,584 + LRK54_RS02535 0.81 +0.2 549,584 + LRK54_RS02535 0.81 +0.8 549,584 + LRK54_RS02535 0.81 -1.0 549,584 + LRK54_RS02535 0.81 +0.5 549,584 + LRK54_RS02535 0.81 +0.6 549,585 - LRK54_RS02535 0.81 +0.4 549,585 - LRK54_RS02535 0.81 -0.2 549,585 - LRK54_RS02535 0.81 +0.6 549,585 - LRK54_RS02535 0.81 +0.2 549,585 - LRK54_RS02535 0.81 +0.2 549,585 - LRK54_RS02535 0.81 -0.1 549,585 - LRK54_RS02535 0.81 +0.5 549,585 - LRK54_RS02535 0.81 +0.5 549,631 - -0.5 549,790 + +1.0 549,790 + -0.2 549,804 + -1.5 549,804 + -1.1 549,804 + -0.5 549,844 - LRK54_RS02540 0.10 -1.4
Or see this region's nucleotide sequence