Experiment: L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS02015 and RR42_RS02020 are separated by 97 nucleotides RR42_RS02020 and RR42_RS02025 are separated by 59 nucleotides RR42_RS02025 and RR42_RS02030 overlap by 4 nucleotides RR42_RS02030 and RR42_RS02035 are separated by 119 nucleotides
RR42_RS02015: RR42_RS02015 - ribose-phosphate pyrophosphokinase, at 449,464 to 450,417
_RS02015
RR42_RS02020: RR42_RS02020 - tRNA-Gln, at 450,515 to 450,591
_RS02020
RR42_RS02025: RR42_RS02025 - 4-diphosphocytidyl-2C-methyl-D-erythritol kinase, at 450,651 to 451,529
_RS02025
RR42_RS02030: RR42_RS02030 - membrane protein, at 451,526 to 452,146
_RS02030
RR42_RS02035: RR42_RS02035 - hypothetical protein, at 452,266 to 454,149
_RS02035
Position (kb) 450
451
452 Strain fitness (log2 ratio) -2
-1
0
1 at 450.513 kb on - strand at 450.513 kb on - strand at 450.513 kb on - strand at 451.820 kb on - strand, within RR42_RS02030 at 452.002 kb on - strand, within RR42_RS02030 at 452.002 kb on - strand, within RR42_RS02030 at 452.267 kb on + strand at 452.267 kb on + strand at 452.296 kb on - strand at 452.296 kb on - strand at 452.298 kb on - strand at 452.339 kb on - strand at 452.339 kb on - strand at 452.523 kb on - strand, within RR42_RS02035 at 452.523 kb on - strand, within RR42_RS02035 at 452.523 kb on - strand, within RR42_RS02035 at 452.523 kb on - strand, within RR42_RS02035
Per-strain Table
Position Strand Gene LocusTag Fraction L-Lactate (C) remove 450,513 - -0.5 450,513 - -2.0 450,513 - +0.9 451,820 - RR42_RS02030 0.47 +0.9 452,002 - RR42_RS02030 0.77 +1.5 452,002 - RR42_RS02030 0.77 -0.7 452,267 + -1.8 452,267 + -0.1 452,296 - -2.0 452,296 - +0.1 452,298 - -0.2 452,339 - -0.7 452,339 - +0.9 452,523 - RR42_RS02035 0.14 -1.5 452,523 - RR42_RS02035 0.14 +0.7 452,523 - RR42_RS02035 0.14 -1.0 452,523 - RR42_RS02035 0.14 +0.3
Or see this region's nucleotide sequence