Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5015 and Pf6N2E2_5016 are separated by 40 nucleotides Pf6N2E2_5016 and Pf6N2E2_5017 overlap by 8 nucleotides Pf6N2E2_5017 and Pf6N2E2_5018 are separated by 215 nucleotides
Pf6N2E2_5015 - Magnesium and cobalt efflux protein CorC, at 5,782,132 to 5,782,971
_5015
Pf6N2E2_5016 - Metal-dependent hydrolase YbeY, involved in rRNA and/or ribosome maturation and assembly, at 5,783,012 to 5,783,470
_5016
Pf6N2E2_5017 - Phosphate starvation-inducible protein PhoH, predicted ATPase, at 5,783,463 to 5,784,473
_5017
Pf6N2E2_5018 - tRNA-i(6)A37 methylthiotransferase, at 5,784,689 to 5,786,053
_5018
Position (kb)
5783
5784
5785 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 5783.011 kb on - strand at 5783.011 kb on - strand at 5783.011 kb on - strand at 5783.011 kb on - strand at 5783.011 kb on - strand at 5783.011 kb on - strand at 5783.011 kb on - strand at 5783.011 kb on - strand at 5783.807 kb on - strand, within Pf6N2E2_5017 at 5783.807 kb on - strand, within Pf6N2E2_5017 at 5783.807 kb on - strand, within Pf6N2E2_5017 at 5783.807 kb on - strand, within Pf6N2E2_5017 at 5783.807 kb on - strand, within Pf6N2E2_5017 at 5783.891 kb on - strand, within Pf6N2E2_5017 at 5783.906 kb on - strand, within Pf6N2E2_5017 at 5783.906 kb on - strand, within Pf6N2E2_5017 at 5784.041 kb on - strand, within Pf6N2E2_5017 at 5784.041 kb on - strand, within Pf6N2E2_5017 at 5784.041 kb on - strand, within Pf6N2E2_5017 at 5784.107 kb on - strand, within Pf6N2E2_5017 at 5784.107 kb on - strand, within Pf6N2E2_5017 at 5784.107 kb on - strand, within Pf6N2E2_5017 at 5784.502 kb on - strand at 5784.528 kb on - strand at 5784.548 kb on + strand at 5784.548 kb on + strand at 5784.634 kb on + strand at 5784.635 kb on - strand at 5784.635 kb on - strand at 5785.005 kb on + strand, within Pf6N2E2_5018 at 5785.005 kb on + strand, within Pf6N2E2_5018 at 5785.006 kb on - strand, within Pf6N2E2_5018 at 5785.006 kb on - strand, within Pf6N2E2_5018 at 5785.017 kb on + strand, within Pf6N2E2_5018 at 5785.017 kb on + strand, within Pf6N2E2_5018 at 5785.018 kb on - strand, within Pf6N2E2_5018 at 5785.018 kb on - strand, within Pf6N2E2_5018 at 5785.018 kb on - strand, within Pf6N2E2_5018 at 5785.100 kb on - strand, within Pf6N2E2_5018 at 5785.100 kb on - strand, within Pf6N2E2_5018 at 5785.161 kb on + strand, within Pf6N2E2_5018
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 5,783,011 - -2.5 5,783,011 - -1.3 5,783,011 - -0.9 5,783,011 - -0.3 5,783,011 - -0.5 5,783,011 - -0.6 5,783,011 - -2.4 5,783,011 - -3.8 5,783,807 - Pf6N2E2_5017 0.34 -0.2 5,783,807 - Pf6N2E2_5017 0.34 -0.5 5,783,807 - Pf6N2E2_5017 0.34 -0.7 5,783,807 - Pf6N2E2_5017 0.34 +0.2 5,783,807 - Pf6N2E2_5017 0.34 -0.2 5,783,891 - Pf6N2E2_5017 0.42 +0.1 5,783,906 - Pf6N2E2_5017 0.44 -1.1 5,783,906 - Pf6N2E2_5017 0.44 -0.8 5,784,041 - Pf6N2E2_5017 0.57 -0.1 5,784,041 - Pf6N2E2_5017 0.57 -0.6 5,784,041 - Pf6N2E2_5017 0.57 -1.8 5,784,107 - Pf6N2E2_5017 0.64 -1.4 5,784,107 - Pf6N2E2_5017 0.64 -0.5 5,784,107 - Pf6N2E2_5017 0.64 -0.0 5,784,502 - -0.3 5,784,528 - -1.5 5,784,548 + -0.0 5,784,548 + +0.4 5,784,634 + +0.1 5,784,635 - -1.3 5,784,635 - -0.9 5,785,005 + Pf6N2E2_5018 0.23 +0.5 5,785,005 + Pf6N2E2_5018 0.23 +0.1 5,785,006 - Pf6N2E2_5018 0.23 +0.2 5,785,006 - Pf6N2E2_5018 0.23 -0.3 5,785,017 + Pf6N2E2_5018 0.24 +0.2 5,785,017 + Pf6N2E2_5018 0.24 +1.1 5,785,018 - Pf6N2E2_5018 0.24 -0.3 5,785,018 - Pf6N2E2_5018 0.24 -0.2 5,785,018 - Pf6N2E2_5018 0.24 -0.7 5,785,100 - Pf6N2E2_5018 0.30 -0.3 5,785,100 - Pf6N2E2_5018 0.30 -0.1 5,785,161 + Pf6N2E2_5018 0.35 +0.1
Or see this region's nucleotide sequence