Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_44

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_43 and Pf6N2E2_44 are separated by 259 nucleotidesPf6N2E2_44 and Pf6N2E2_45 are separated by 177 nucleotides Pf6N2E2_43 - Histidine permease YuiF, at 81,349 to 82,668 _43 Pf6N2E2_44 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 82,928 to 84,607 _44 Pf6N2E2_45 - Putative protease, at 84,785 to 86,209 _45 Position (kb) 82 83 84 85Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 82.003 kb on + strand, within Pf6N2E2_43at 82.049 kb on - strand, within Pf6N2E2_43at 82.619 kb on - strandat 82.619 kb on - strandat 82.619 kb on - strandat 82.619 kb on - strandat 82.619 kb on - strandat 82.666 kb on + strandat 82.667 kb on - strandat 82.667 kb on - strandat 82.667 kb on - strandat 82.753 kb on + strandat 82.753 kb on + strandat 82.753 kb on + strandat 82.754 kb on - strandat 82.754 kb on - strandat 82.754 kb on - strandat 82.754 kb on - strandat 82.754 kb on - strandat 82.828 kb on + strandat 82.829 kb on - strandat 82.829 kb on - strandat 82.829 kb on - strandat 82.829 kb on - strandat 82.829 kb on - strandat 82.829 kb on - strandat 82.834 kb on + strandat 82.861 kb on - strandat 82.861 kb on - strandat 82.887 kb on + strandat 82.887 kb on + strandat 82.888 kb on - strandat 82.888 kb on - strandat 82.888 kb on - strandat 82.905 kb on + strandat 82.906 kb on - strandat 82.912 kb on + strandat 82.912 kb on + strandat 82.912 kb on + strandat 82.913 kb on - strandat 82.948 kb on + strandat 83.035 kb on + strandat 83.035 kb on + strandat 83.036 kb on - strandat 83.099 kb on + strand, within Pf6N2E2_44at 83.100 kb on - strand, within Pf6N2E2_44at 83.100 kb on - strand, within Pf6N2E2_44at 83.136 kb on - strandat 83.158 kb on + strand, within Pf6N2E2_44at 83.204 kb on + strand, within Pf6N2E2_44at 83.220 kb on - strand, within Pf6N2E2_44at 83.321 kb on + strand, within Pf6N2E2_44at 83.321 kb on + strand, within Pf6N2E2_44at 83.322 kb on - strand, within Pf6N2E2_44at 83.366 kb on + strand, within Pf6N2E2_44at 83.367 kb on - strand, within Pf6N2E2_44at 83.367 kb on - strand, within Pf6N2E2_44at 83.375 kb on + strand, within Pf6N2E2_44at 83.375 kb on + strand, within Pf6N2E2_44at 83.432 kb on + strand, within Pf6N2E2_44at 83.432 kb on + strand, within Pf6N2E2_44at 83.432 kb on + strand, within Pf6N2E2_44at 83.433 kb on - strand, within Pf6N2E2_44at 83.529 kb on + strand, within Pf6N2E2_44at 83.529 kb on + strand, within Pf6N2E2_44at 83.529 kb on + strand, within Pf6N2E2_44at 83.530 kb on - strand, within Pf6N2E2_44at 83.530 kb on - strand, within Pf6N2E2_44at 84.219 kb on + strand, within Pf6N2E2_44at 84.219 kb on + strand, within Pf6N2E2_44at 84.219 kb on + strand, within Pf6N2E2_44at 84.220 kb on - strand, within Pf6N2E2_44at 84.220 kb on - strand, within Pf6N2E2_44at 84.858 kb on + strandat 84.859 kb on - strandat 85.081 kb on - strand, within Pf6N2E2_45at 85.081 kb on - strand, within Pf6N2E2_45at 85.081 kb on - strand, within Pf6N2E2_45at 85.089 kb on + strand, within Pf6N2E2_45at 85.090 kb on - strand, within Pf6N2E2_45at 85.239 kb on + strand, within Pf6N2E2_45at 85.239 kb on + strand, within Pf6N2E2_45at 85.240 kb on - strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.344 kb on + strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.345 kb on - strand, within Pf6N2E2_45at 85.458 kb on + strand, within Pf6N2E2_45at 85.458 kb on + strand, within Pf6N2E2_45at 85.458 kb on + strand, within Pf6N2E2_45at 85.459 kb on - strand, within Pf6N2E2_45at 85.459 kb on - strand, within Pf6N2E2_45at 85.459 kb on - strand, within Pf6N2E2_45at 85.459 kb on - strand, within Pf6N2E2_45at 85.459 kb on - strand, within Pf6N2E2_45at 85.483 kb on + strand, within Pf6N2E2_45at 85.510 kb on - strand, within Pf6N2E2_45at 85.510 kb on - strand, within Pf6N2E2_45at 85.562 kb on + strand, within Pf6N2E2_45at 85.562 kb on + strand, within Pf6N2E2_45at 85.562 kb on + strand, within Pf6N2E2_45at 85.593 kb on + strand, within Pf6N2E2_45at 85.594 kb on - strand, within Pf6N2E2_45

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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82,003 + Pf6N2E2_43 0.50 -3.3
82,049 - Pf6N2E2_43 0.53 -3.2
82,619 - -3.6
82,619 - -2.0
82,619 - -2.6
82,619 - -3.4
82,619 - -3.3
82,666 + +0.6
82,667 - -0.5
82,667 - -1.5
82,667 - -0.5
82,753 + -0.5
82,753 + -0.7
82,753 + -0.3
82,754 - +0.4
82,754 - +0.8
82,754 - -0.1
82,754 - +0.3
82,754 - +0.0
82,828 + +0.1
82,829 - -0.8
82,829 - +0.0
82,829 - +0.5
82,829 - -0.5
82,829 - +0.3
82,829 - -0.2
82,834 + +0.9
82,861 - -1.0
82,861 - -0.3
82,887 + -0.4
82,887 + +0.2
82,888 - +0.2
82,888 - +0.7
82,888 - -0.5
82,905 + +0.9
82,906 - +0.5
82,912 + +0.1
82,912 + -0.3
82,912 + -1.1
82,913 - +0.0
82,948 + -0.3
83,035 + +2.3
83,035 + +1.0
83,036 - +1.0
83,099 + Pf6N2E2_44 0.10 +0.9
83,100 - Pf6N2E2_44 0.10 +0.8
83,100 - Pf6N2E2_44 0.10 -0.8
83,136 - +0.7
83,158 + Pf6N2E2_44 0.14 +0.5
83,204 + Pf6N2E2_44 0.16 -1.2
83,220 - Pf6N2E2_44 0.17 +2.7
83,321 + Pf6N2E2_44 0.23 +1.0
83,321 + Pf6N2E2_44 0.23 -1.0
83,322 - Pf6N2E2_44 0.23 -0.3
83,366 + Pf6N2E2_44 0.26 -0.1
83,367 - Pf6N2E2_44 0.26 +0.1
83,367 - Pf6N2E2_44 0.26 +0.1
83,375 + Pf6N2E2_44 0.27 -0.1
83,375 + Pf6N2E2_44 0.27 +0.9
83,432 + Pf6N2E2_44 0.30 -0.8
83,432 + Pf6N2E2_44 0.30 -0.3
83,432 + Pf6N2E2_44 0.30 +0.3
83,433 - Pf6N2E2_44 0.30 +0.4
83,529 + Pf6N2E2_44 0.36 +0.4
83,529 + Pf6N2E2_44 0.36 -0.2
83,529 + Pf6N2E2_44 0.36 -2.0
83,530 - Pf6N2E2_44 0.36 +0.3
83,530 - Pf6N2E2_44 0.36 -0.1
84,219 + Pf6N2E2_44 0.77 +1.6
84,219 + Pf6N2E2_44 0.77 +0.4
84,219 + Pf6N2E2_44 0.77 +0.6
84,220 - Pf6N2E2_44 0.77 +0.6
84,220 - Pf6N2E2_44 0.77 -0.4
84,858 + +0.3
84,859 - +0.7
85,081 - Pf6N2E2_45 0.21 -0.5
85,081 - Pf6N2E2_45 0.21 -0.0
85,081 - Pf6N2E2_45 0.21 +0.4
85,089 + Pf6N2E2_45 0.21 +0.5
85,090 - Pf6N2E2_45 0.21 -0.5
85,239 + Pf6N2E2_45 0.32 +0.2
85,239 + Pf6N2E2_45 0.32 +0.2
85,240 - Pf6N2E2_45 0.32 +0.3
85,344 + Pf6N2E2_45 0.39 +0.5
85,344 + Pf6N2E2_45 0.39 -0.2
85,344 + Pf6N2E2_45 0.39 +1.7
85,344 + Pf6N2E2_45 0.39 -0.1
85,344 + Pf6N2E2_45 0.39 -0.2
85,344 + Pf6N2E2_45 0.39 +1.2
85,344 + Pf6N2E2_45 0.39 +0.4
85,345 - Pf6N2E2_45 0.39 -0.4
85,345 - Pf6N2E2_45 0.39 -0.9
85,345 - Pf6N2E2_45 0.39 -0.0
85,345 - Pf6N2E2_45 0.39 +1.3
85,345 - Pf6N2E2_45 0.39 +1.1
85,345 - Pf6N2E2_45 0.39 +1.1
85,458 + Pf6N2E2_45 0.47 +0.0
85,458 + Pf6N2E2_45 0.47 +0.1
85,458 + Pf6N2E2_45 0.47 -1.2
85,459 - Pf6N2E2_45 0.47 +0.3
85,459 - Pf6N2E2_45 0.47 -1.3
85,459 - Pf6N2E2_45 0.47 -0.3
85,459 - Pf6N2E2_45 0.47 +0.6
85,459 - Pf6N2E2_45 0.47 -1.2
85,483 + Pf6N2E2_45 0.49 +1.3
85,510 - Pf6N2E2_45 0.51 +0.7
85,510 - Pf6N2E2_45 0.51 -1.2
85,562 + Pf6N2E2_45 0.55 +0.6
85,562 + Pf6N2E2_45 0.55 -0.2
85,562 + Pf6N2E2_45 0.55 +0.3
85,593 + Pf6N2E2_45 0.57 -0.3
85,594 - Pf6N2E2_45 0.57 +1.3

Or see this region's nucleotide sequence