Experiment: Nickel (II) chloride 1 mM 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf6N2E2_3653 and Pf6N2E2_3654 are separated by 85 nucleotides Pf6N2E2_3654 and Pf6N2E2_3655 are separated by 268 nucleotides Pf6N2E2_3655 and Pf6N2E2_3656 are separated by 16 nucleotides Pf6N2E2_3656 and Pf6N2E2_3657 overlap by 4 nucleotides 
        Pf6N2E2_3653 - ADP-heptose synthase (EC 2.7.-.-) / D-glycero-beta-D-manno-heptose 7-phosphate kinase, at 4,253,445 to 4,254,866 
        _3653 
         
        
        Pf6N2E2_3654 - Adenylylsulfate kinase (EC 2.7.1.25), at 4,254,952 to 4,255,413 
        _3654 
         
        
        Pf6N2E2_3655 - Hypothetical protein FIG015671 in large core OS assembly cluster, at 4,255,682 to 4,257,097 
        _3655 
         
        
        Pf6N2E2_3656 - Probable transcription regulator Mig-14, at 4,257,114 to 4,258,013 
        _3656 
         
        
        Pf6N2E2_3657 - Lipid A export ATP-binding/permease protein MsbA, at 4,258,010 to 4,259,800 
        _3657 
         Position (kb) 4255 
4256 
4257 
4258 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 
2 at 4254.881 kb on - strand at 4254.906 kb on - strand at 4254.906 kb on - strand at 4254.906 kb on - strand at 4254.906 kb on - strand at 4254.906 kb on - strand at 4255.046 kb on + strand, within Pf6N2E2_3654 at 4255.047 kb on - strand, within Pf6N2E2_3654 at 4255.100 kb on + strand, within Pf6N2E2_3654 at 4255.151 kb on + strand, within Pf6N2E2_3654 at 4255.151 kb on + strand, within Pf6N2E2_3654 at 4255.151 kb on + strand, within Pf6N2E2_3654 at 4255.152 kb on - strand, within Pf6N2E2_3654 at 4255.152 kb on - strand, within Pf6N2E2_3654 at 4255.330 kb on + strand, within Pf6N2E2_3654 at 4255.330 kb on + strand, within Pf6N2E2_3654 at 4255.330 kb on + strand, within Pf6N2E2_3654 at 4255.330 kb on + strand, within Pf6N2E2_3654 at 4255.331 kb on - strand, within Pf6N2E2_3654 at 4255.331 kb on - strand, within Pf6N2E2_3654 at 4255.378 kb on + strand at 4255.379 kb on - strand at 4255.452 kb on + strand at 4255.453 kb on - strand at 4255.453 kb on - strand at 4255.453 kb on - strand at 4255.521 kb on + strand at 4255.540 kb on + strand at 4255.624 kb on + strand at 4255.624 kb on + strand at 4255.626 kb on + strand at 4255.634 kb on + strand at 4255.653 kb on + strand at 4255.653 kb on + strand at 4255.654 kb on - strand at 4255.654 kb on - strand at 4255.866 kb on - strand, within Pf6N2E2_3655 at 4255.916 kb on + strand at 4256.016 kb on + strand, within Pf6N2E2_3655 at 4256.017 kb on - strand, within Pf6N2E2_3655 at 4256.017 kb on - strand, within Pf6N2E2_3655 at 4256.017 kb on - strand, within Pf6N2E2_3655 at 4256.017 kb on - strand, within Pf6N2E2_3655 at 4256.029 kb on + strand, within Pf6N2E2_3655 at 4256.044 kb on + strand, within Pf6N2E2_3655 at 4256.045 kb on - strand, within Pf6N2E2_3655 at 4256.045 kb on - strand, within Pf6N2E2_3655 at 4256.160 kb on - strand, within Pf6N2E2_3655 at 4256.160 kb on - strand, within Pf6N2E2_3655 at 4256.248 kb on + strand, within Pf6N2E2_3655 at 4256.249 kb on - strand, within Pf6N2E2_3655 at 4256.249 kb on - strand, within Pf6N2E2_3655 at 4256.255 kb on - strand, within Pf6N2E2_3655 at 4256.322 kb on + strand, within Pf6N2E2_3655 at 4256.322 kb on + strand, within Pf6N2E2_3655 at 4256.322 kb on + strand, within Pf6N2E2_3655 at 4256.322 kb on + strand, within Pf6N2E2_3655 at 4256.322 kb on + strand, within Pf6N2E2_3655 at 4256.323 kb on - strand, within Pf6N2E2_3655 at 4256.323 kb on - strand, within Pf6N2E2_3655 at 4256.323 kb on - strand, within Pf6N2E2_3655 at 4256.325 kb on + strand, within Pf6N2E2_3655 at 4256.448 kb on + strand, within Pf6N2E2_3655 at 4256.449 kb on - strand, within Pf6N2E2_3655 at 4256.449 kb on - strand, within Pf6N2E2_3655 at 4256.453 kb on - strand, within Pf6N2E2_3655 at 4256.489 kb on - strand, within Pf6N2E2_3655 at 4256.510 kb on - strand, within Pf6N2E2_3655 at 4256.511 kb on + strand, within Pf6N2E2_3655 at 4256.512 kb on - strand, within Pf6N2E2_3655 at 4256.512 kb on - strand, within Pf6N2E2_3655 at 4256.512 kb on - strand, within Pf6N2E2_3655 at 4256.512 kb on - strand, within Pf6N2E2_3655 at 4256.560 kb on + strand, within Pf6N2E2_3655 at 4256.560 kb on + strand, within Pf6N2E2_3655 at 4256.747 kb on + strand, within Pf6N2E2_3655 at 4256.748 kb on - strand, within Pf6N2E2_3655 at 4256.748 kb on - strand, within Pf6N2E2_3655 at 4256.896 kb on - strand, within Pf6N2E2_3655 at 4256.896 kb on - strand, within Pf6N2E2_3655 at 4256.901 kb on + strand, within Pf6N2E2_3655 at 4256.901 kb on + strand, within Pf6N2E2_3655 at 4256.919 kb on + strand, within Pf6N2E2_3655 at 4256.919 kb on + strand, within Pf6N2E2_3655 at 4256.919 kb on + strand, within Pf6N2E2_3655 at 4256.919 kb on + strand, within Pf6N2E2_3655 at 4256.920 kb on - strand, within Pf6N2E2_3655 at 4256.920 kb on - strand, within Pf6N2E2_3655 at 4256.920 kb on - strand, within Pf6N2E2_3655 at 4256.920 kb on - strand, within Pf6N2E2_3655 at 4256.920 kb on - strand, within Pf6N2E2_3655 at 4256.920 kb on - strand, within Pf6N2E2_3655 at 4257.042 kb on - strand at 4257.124 kb on + strand at 4257.124 kb on + strand at 4257.157 kb on + strand at 4257.157 kb on + strand at 4257.301 kb on + strand, within Pf6N2E2_3656 at 4257.301 kb on + strand, within Pf6N2E2_3656 at 4257.301 kb on + strand, within Pf6N2E2_3656 at 4257.301 kb on + strand, within Pf6N2E2_3656 at 4257.301 kb on + strand, within Pf6N2E2_3656 at 4257.373 kb on + strand, within Pf6N2E2_3656 at 4257.478 kb on + strand, within Pf6N2E2_3656 at 4257.478 kb on + strand, within Pf6N2E2_3656 at 4257.478 kb on + strand, within Pf6N2E2_3656 at 4257.478 kb on + strand, within Pf6N2E2_3656 at 4257.478 kb on + strand, within Pf6N2E2_3656 at 4257.478 kb on + strand, within Pf6N2E2_3656 at 4257.515 kb on + strand, within Pf6N2E2_3656 at 4257.515 kb on + strand, within Pf6N2E2_3656 at 4257.515 kb on + strand, within Pf6N2E2_3656 at 4257.584 kb on + strand, within Pf6N2E2_3656 at 4257.584 kb on + strand, within Pf6N2E2_3656 at 4257.589 kb on + strand, within Pf6N2E2_3656 at 4257.589 kb on + strand, within Pf6N2E2_3656 at 4257.673 kb on + strand, within Pf6N2E2_3656 at 4257.673 kb on + strand, within Pf6N2E2_3656 at 4257.673 kb on + strand, within Pf6N2E2_3656 at 4257.673 kb on + strand at 4257.673 kb on + strand, within Pf6N2E2_3656 at 4257.673 kb on + strand, within Pf6N2E2_3656 at 4257.856 kb on + strand, within Pf6N2E2_3656 at 4257.949 kb on + strand  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Nickel (II) chloride 1 mM remove 4,254,881 -  +0.5 4,254,906 -  -0.2 4,254,906 -  +0.2 4,254,906 -  +0.9 4,254,906 -  +0.3 4,254,906 -  +0.1 4,255,046 +  Pf6N2E2_3654 0.20  +0.5 4,255,047 -  Pf6N2E2_3654 0.21  +0.8 4,255,100 +  Pf6N2E2_3654 0.32  +0.5 4,255,151 +  Pf6N2E2_3654 0.43  +0.3 4,255,151 +  Pf6N2E2_3654 0.43  -0.0 4,255,151 +  Pf6N2E2_3654 0.43  -1.1 4,255,152 -  Pf6N2E2_3654 0.43  -0.4 4,255,152 -  Pf6N2E2_3654 0.43  +0.3 4,255,330 +  Pf6N2E2_3654 0.82  +0.7 4,255,330 +  Pf6N2E2_3654 0.82  -0.4 4,255,330 +  Pf6N2E2_3654 0.82  +0.7 4,255,330 +  Pf6N2E2_3654 0.82  -0.4 4,255,331 -  Pf6N2E2_3654 0.82  +0.3 4,255,331 -  Pf6N2E2_3654 0.82  +0.2 4,255,378 +  +0.5 4,255,379 -  +0.4 4,255,452 +  +1.2 4,255,453 -  +0.2 4,255,453 -  +0.4 4,255,453 -  +0.5 4,255,521 +  -0.1 4,255,540 +  +1.0 4,255,624 +  -0.7 4,255,624 +  -1.7 4,255,626 +  +0.2 4,255,634 +  +0.8 4,255,653 +  +0.3 4,255,653 +  +0.2 4,255,654 -  -1.2 4,255,654 -  -0.5 4,255,866 -  Pf6N2E2_3655 0.13  -0.2 4,255,916 +  +0.7 4,256,016 +  Pf6N2E2_3655 0.24  -0.9 4,256,017 -  Pf6N2E2_3655 0.24  +0.5 4,256,017 -  Pf6N2E2_3655 0.24  -0.1 4,256,017 -  Pf6N2E2_3655 0.24  -0.7 4,256,017 -  Pf6N2E2_3655 0.24  -0.0 4,256,029 +  Pf6N2E2_3655 0.25  -1.0 4,256,044 +  Pf6N2E2_3655 0.26  -2.1 4,256,045 -  Pf6N2E2_3655 0.26  -0.7 4,256,045 -  Pf6N2E2_3655 0.26  -0.7 4,256,160 -  Pf6N2E2_3655 0.34  +0.3 4,256,160 -  Pf6N2E2_3655 0.34  -0.3 4,256,248 +  Pf6N2E2_3655 0.40  +0.5 4,256,249 -  Pf6N2E2_3655 0.40  -0.9 4,256,249 -  Pf6N2E2_3655 0.40  +0.0 4,256,255 -  Pf6N2E2_3655 0.40  -0.3 4,256,322 +  Pf6N2E2_3655 0.45  +1.7 4,256,322 +  Pf6N2E2_3655 0.45  -0.1 4,256,322 +  Pf6N2E2_3655 0.45  -0.0 4,256,322 +  Pf6N2E2_3655 0.45  +0.0 4,256,322 +  Pf6N2E2_3655 0.45  +0.0 4,256,323 -  Pf6N2E2_3655 0.45  -0.4 4,256,323 -  Pf6N2E2_3655 0.45  -0.6 4,256,323 -  Pf6N2E2_3655 0.45  -0.5 4,256,325 +  Pf6N2E2_3655 0.45  +0.2 4,256,448 +  Pf6N2E2_3655 0.54  -1.1 4,256,449 -  Pf6N2E2_3655 0.54  +0.4 4,256,449 -  Pf6N2E2_3655 0.54  -1.1 4,256,453 -  Pf6N2E2_3655 0.54  -1.6 4,256,489 -  Pf6N2E2_3655 0.57  +0.3 4,256,510 -  Pf6N2E2_3655 0.58  -0.5 4,256,511 +  Pf6N2E2_3655 0.59  -0.5 4,256,512 -  Pf6N2E2_3655 0.59  -2.3 4,256,512 -  Pf6N2E2_3655 0.59  -0.1 4,256,512 -  Pf6N2E2_3655 0.59  -0.2 4,256,512 -  Pf6N2E2_3655 0.59  -1.2 4,256,560 +  Pf6N2E2_3655 0.62  -0.4 4,256,560 +  Pf6N2E2_3655 0.62  +0.3 4,256,747 +  Pf6N2E2_3655 0.75  -1.4 4,256,748 -  Pf6N2E2_3655 0.75  -0.4 4,256,748 -  Pf6N2E2_3655 0.75  -0.5 4,256,896 -  Pf6N2E2_3655 0.86  -0.9 4,256,896 -  Pf6N2E2_3655 0.86  -0.3 4,256,901 +  Pf6N2E2_3655 0.86  -0.5 4,256,901 +  Pf6N2E2_3655 0.86  -0.6 4,256,919 +  Pf6N2E2_3655 0.87  -1.5 4,256,919 +  Pf6N2E2_3655 0.87  -0.2 4,256,919 +  Pf6N2E2_3655 0.87  -1.1 4,256,919 +  Pf6N2E2_3655 0.87  -2.2 4,256,920 -  Pf6N2E2_3655 0.87  +1.2 4,256,920 -  Pf6N2E2_3655 0.87  +0.0 4,256,920 -  Pf6N2E2_3655 0.87  -0.8 4,256,920 -  Pf6N2E2_3655 0.87  -0.6 4,256,920 -  Pf6N2E2_3655 0.87  -1.1 4,256,920 -  Pf6N2E2_3655 0.87  -0.1 4,257,042 -  -0.5 4,257,124 +  -0.3 4,257,124 +  -2.5 4,257,157 +  +0.8 4,257,157 +  -1.2 4,257,301 +  Pf6N2E2_3656 0.21  +0.5 4,257,301 +  Pf6N2E2_3656 0.21  +1.3 4,257,301 +  Pf6N2E2_3656 0.21  -1.1 4,257,301 +  Pf6N2E2_3656 0.21  -0.4 4,257,301 +  Pf6N2E2_3656 0.21  +0.6 4,257,373 +  Pf6N2E2_3656 0.29  -1.5 4,257,478 +  Pf6N2E2_3656 0.40  -0.4 4,257,478 +  Pf6N2E2_3656 0.40  -0.1 4,257,478 +  Pf6N2E2_3656 0.40  -2.8 4,257,478 +  Pf6N2E2_3656 0.40  +0.6 4,257,478 +  Pf6N2E2_3656 0.40  -1.6 4,257,478 +  Pf6N2E2_3656 0.40  -0.2 4,257,515 +  Pf6N2E2_3656 0.45  -1.2 4,257,515 +  Pf6N2E2_3656 0.45  -0.7 4,257,515 +  Pf6N2E2_3656 0.45  -1.7 4,257,584 +  Pf6N2E2_3656 0.52  -0.7 4,257,584 +  Pf6N2E2_3656 0.52  -2.6 4,257,589 +  Pf6N2E2_3656 0.53  -0.5 4,257,589 +  Pf6N2E2_3656 0.53  -1.5 4,257,673 +  Pf6N2E2_3656 0.62  -1.1 4,257,673 +  Pf6N2E2_3656 0.62  -1.1 4,257,673 +  Pf6N2E2_3656 0.62  -0.5 4,257,673 +  +1.8 4,257,673 +  Pf6N2E2_3656 0.62  -0.4 4,257,673 +  Pf6N2E2_3656 0.62  -0.6 4,257,856 +  Pf6N2E2_3656 0.82  -1.1 4,257,949 +  -3.6 
Or see this region's nucleotide sequence