Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3461

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3460 and Pf6N2E2_3461 overlap by 4 nucleotidesPf6N2E2_3461 and Pf6N2E2_6120 are separated by 154 nucleotidesPf6N2E2_6120 and Pf6N2E2_6121 are separated by 167 nucleotides Pf6N2E2_3460 - Exodeoxyribonuclease V beta chain (EC 3.1.11.5), at 4,029,403 to 4,033,092 _3460 Pf6N2E2_3461 - Exodeoxyribonuclease V gamma chain (EC 3.1.11.5), at 4,033,089 to 4,036,541 _3461 Pf6N2E2_6120 - 5S RNA, at 4,036,696 to 4,036,815 _6120 Pf6N2E2_6121 - Large Subunit Ribosomal RNA; lsuRNA; LSU rRNA, at 4,036,983 to 4,039,873 _6121 Position (kb) 4033 4034 4035 4036 4037Strain fitness (log2 ratio) -1 0 1at 4034.108 kb on - strand, within Pf6N2E2_3461at 4035.483 kb on - strand, within Pf6N2E2_3461at 4036.584 kb on - strandat 4036.584 kb on - strandat 4036.631 kb on + strandat 4036.631 kb on + strandat 4036.632 kb on - strandat 4037.132 kb on - strandat 4037.132 kb on - strandat 4037.132 kb on - strandat 4037.132 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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4,034,108 - Pf6N2E2_3461 0.30 -1.4
4,035,483 - Pf6N2E2_3461 0.69 -1.6
4,036,584 - -0.1
4,036,584 - -0.9
4,036,631 + -0.9
4,036,631 + +0.2
4,036,632 - -0.5
4,037,132 - +0.5
4,037,132 - -0.2
4,037,132 - -0.5
4,037,132 - +0.0

Or see this region's nucleotide sequence