Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_32

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_31 and Pf6N2E2_32 are separated by 204 nucleotidesPf6N2E2_32 and Pf6N2E2_33 are separated by 179 nucleotides Pf6N2E2_31 - Iron(III) dicitrate transport protein FecA @ Iron siderophore receptor protein, at 68,024 to 70,462 _31 Pf6N2E2_32 - Membrane protein mosC, at 70,667 to 71,818 _32 Pf6N2E2_33 - Diaminopimelate epimerase (EC 5.1.1.7), at 71,998 to 72,999 _33 Position (kb) 70 71 72Strain fitness (log2 ratio) -2 -1 0 1 2at 69.686 kb on + strand, within Pf6N2E2_31at 69.686 kb on + strand, within Pf6N2E2_31at 69.686 kb on + strand, within Pf6N2E2_31at 69.686 kb on + strand, within Pf6N2E2_31at 69.686 kb on + strand, within Pf6N2E2_31at 69.686 kb on + strand, within Pf6N2E2_31at 69.686 kb on + strand, within Pf6N2E2_31at 69.686 kb on + strand, within Pf6N2E2_31at 69.686 kb on + strand, within Pf6N2E2_31at 69.687 kb on - strand, within Pf6N2E2_31at 69.687 kb on - strand, within Pf6N2E2_31at 69.687 kb on - strand, within Pf6N2E2_31at 69.687 kb on - strand, within Pf6N2E2_31at 69.687 kb on - strand, within Pf6N2E2_31at 69.687 kb on - strand, within Pf6N2E2_31at 69.687 kb on - strand, within Pf6N2E2_31at 69.687 kb on - strand, within Pf6N2E2_31at 69.687 kb on - strand, within Pf6N2E2_31at 69.687 kb on - strand, within Pf6N2E2_31at 69.687 kb on - strand, within Pf6N2E2_31at 69.687 kb on - strand, within Pf6N2E2_31at 69.722 kb on + strand, within Pf6N2E2_31at 69.723 kb on - strand, within Pf6N2E2_31at 69.723 kb on - strand, within Pf6N2E2_31at 69.749 kb on + strand, within Pf6N2E2_31at 69.821 kb on + strand, within Pf6N2E2_31at 69.822 kb on - strand, within Pf6N2E2_31at 69.839 kb on + strand, within Pf6N2E2_31at 69.840 kb on - strand, within Pf6N2E2_31at 69.878 kb on + strand, within Pf6N2E2_31at 69.878 kb on + strand, within Pf6N2E2_31at 69.879 kb on - strand, within Pf6N2E2_31at 69.879 kb on - strand, within Pf6N2E2_31at 69.906 kb on - strand, within Pf6N2E2_31at 69.906 kb on - strand, within Pf6N2E2_31at 69.978 kb on - strand, within Pf6N2E2_31at 69.978 kb on - strand, within Pf6N2E2_31at 70.038 kb on - strand, within Pf6N2E2_31at 70.052 kb on + strand, within Pf6N2E2_31at 70.052 kb on + strand, within Pf6N2E2_31at 70.052 kb on + strand, within Pf6N2E2_31at 70.052 kb on + strand, within Pf6N2E2_31at 70.053 kb on - strand, within Pf6N2E2_31at 70.053 kb on - strand, within Pf6N2E2_31at 70.097 kb on + strand, within Pf6N2E2_31at 70.098 kb on - strand, within Pf6N2E2_31at 70.130 kb on + strand, within Pf6N2E2_31at 70.151 kb on + strand, within Pf6N2E2_31at 70.151 kb on + strand, within Pf6N2E2_31at 70.151 kb on + strand, within Pf6N2E2_31at 70.152 kb on - strand, within Pf6N2E2_31at 70.152 kb on - strand, within Pf6N2E2_31at 70.152 kb on - strand, within Pf6N2E2_31at 70.232 kb on + strandat 70.232 kb on + strandat 70.232 kb on + strandat 70.233 kb on - strandat 70.233 kb on - strandat 70.233 kb on - strandat 70.233 kb on - strandat 70.233 kb on - strandat 70.280 kb on + strandat 70.307 kb on + strandat 70.376 kb on + strandat 70.376 kb on + strandat 70.377 kb on - strandat 70.377 kb on - strandat 70.379 kb on + strandat 70.379 kb on + strandat 70.379 kb on + strandat 70.379 kb on + strandat 70.380 kb on - strandat 70.380 kb on - strandat 70.380 kb on - strandat 70.380 kb on - strandat 70.380 kb on - strandat 70.380 kb on - strandat 70.412 kb on + strandat 70.412 kb on + strandat 70.413 kb on - strandat 70.413 kb on - strandat 70.413 kb on - strandat 70.429 kb on + strandat 70.480 kb on + strandat 70.480 kb on + strandat 70.480 kb on + strandat 70.481 kb on - strandat 70.498 kb on + strandat 70.498 kb on + strandat 70.511 kb on - strandat 70.841 kb on + strand, within Pf6N2E2_32at 70.842 kb on - strand, within Pf6N2E2_32at 70.842 kb on - strand, within Pf6N2E2_32at 70.866 kb on + strand, within Pf6N2E2_32at 70.866 kb on + strand, within Pf6N2E2_32at 70.920 kb on - strand, within Pf6N2E2_32at 70.920 kb on - strand, within Pf6N2E2_32at 70.986 kb on + strand, within Pf6N2E2_32at 70.987 kb on - strand, within Pf6N2E2_32at 70.987 kb on - strand, within Pf6N2E2_32at 71.018 kb on + strand, within Pf6N2E2_32at 71.018 kb on + strand, within Pf6N2E2_32at 71.019 kb on - strand, within Pf6N2E2_32at 71.019 kb on - strand, within Pf6N2E2_32at 71.019 kb on - strand, within Pf6N2E2_32at 71.019 kb on - strand, within Pf6N2E2_32at 71.073 kb on + strand, within Pf6N2E2_32at 71.282 kb on + strand, within Pf6N2E2_32at 71.283 kb on - strand, within Pf6N2E2_32at 71.834 kb on + strandat 71.835 kb on - strandat 71.865 kb on + strandat 71.865 kb on + strandat 71.866 kb on - strandat 71.866 kb on - strandat 71.866 kb on - strandat 71.959 kb on + strandat 71.959 kb on + strandat 71.960 kb on - strandat 71.960 kb on - strandat 71.960 kb on - strandat 71.960 kb on - strandat 71.960 kb on - strandat 71.964 kb on + strandat 72.011 kb on - strandat 72.011 kb on - strandat 72.027 kb on + strandat 72.028 kb on - strandat 72.028 kb on - strandat 72.028 kb on - strandat 72.028 kb on - strandat 72.028 kb on - strandat 72.028 kb on - strandat 72.028 kb on - strandat 72.028 kb on - strandat 72.031 kb on + strandat 72.031 kb on + strandat 72.031 kb on + strandat 72.031 kb on + strandat 72.032 kb on - strandat 72.032 kb on - strandat 72.255 kb on + strand, within Pf6N2E2_33at 72.694 kb on + strand, within Pf6N2E2_33at 72.695 kb on - strand, within Pf6N2E2_33

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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69,686 + Pf6N2E2_31 0.68 +1.1
69,686 + Pf6N2E2_31 0.68 +0.3
69,686 + Pf6N2E2_31 0.68 +0.1
69,686 + Pf6N2E2_31 0.68 +0.4
69,686 + Pf6N2E2_31 0.68 +0.1
69,686 + Pf6N2E2_31 0.68 -0.4
69,686 + Pf6N2E2_31 0.68 -0.1
69,686 + Pf6N2E2_31 0.68 +0.1
69,686 + Pf6N2E2_31 0.68 -0.5
69,687 - Pf6N2E2_31 0.68 +1.1
69,687 - Pf6N2E2_31 0.68 +1.3
69,687 - Pf6N2E2_31 0.68 +0.4
69,687 - Pf6N2E2_31 0.68 -0.5
69,687 - Pf6N2E2_31 0.68 +0.9
69,687 - Pf6N2E2_31 0.68 +0.9
69,687 - Pf6N2E2_31 0.68 +0.1
69,687 - Pf6N2E2_31 0.68 -0.5
69,687 - Pf6N2E2_31 0.68 +0.1
69,687 - Pf6N2E2_31 0.68 +1.2
69,687 - Pf6N2E2_31 0.68 -0.4
69,687 - Pf6N2E2_31 0.68 +0.5
69,722 + Pf6N2E2_31 0.70 +0.5
69,723 - Pf6N2E2_31 0.70 -0.1
69,723 - Pf6N2E2_31 0.70 +0.9
69,749 + Pf6N2E2_31 0.71 +0.3
69,821 + Pf6N2E2_31 0.74 +0.2
69,822 - Pf6N2E2_31 0.74 +0.7
69,839 + Pf6N2E2_31 0.74 +0.2
69,840 - Pf6N2E2_31 0.74 -0.3
69,878 + Pf6N2E2_31 0.76 -0.3
69,878 + Pf6N2E2_31 0.76 -0.1
69,879 - Pf6N2E2_31 0.76 +0.5
69,879 - Pf6N2E2_31 0.76 -0.7
69,906 - Pf6N2E2_31 0.77 -0.4
69,906 - Pf6N2E2_31 0.77 +0.4
69,978 - Pf6N2E2_31 0.80 -0.8
69,978 - Pf6N2E2_31 0.80 +0.2
70,038 - Pf6N2E2_31 0.83 -0.3
70,052 + Pf6N2E2_31 0.83 +0.2
70,052 + Pf6N2E2_31 0.83 -0.7
70,052 + Pf6N2E2_31 0.83 +0.0
70,052 + Pf6N2E2_31 0.83 +0.0
70,053 - Pf6N2E2_31 0.83 -0.6
70,053 - Pf6N2E2_31 0.83 -0.8
70,097 + Pf6N2E2_31 0.85 -2.1
70,098 - Pf6N2E2_31 0.85 +0.8
70,130 + Pf6N2E2_31 0.86 -0.3
70,151 + Pf6N2E2_31 0.87 +0.4
70,151 + Pf6N2E2_31 0.87 +0.1
70,151 + Pf6N2E2_31 0.87 -0.7
70,152 - Pf6N2E2_31 0.87 +0.4
70,152 - Pf6N2E2_31 0.87 +0.8
70,152 - Pf6N2E2_31 0.87 -0.1
70,232 + +0.1
70,232 + +0.0
70,232 + +0.2
70,233 - -0.9
70,233 - +1.0
70,233 - -0.2
70,233 - +0.3
70,233 - +0.4
70,280 + -0.1
70,307 + +0.6
70,376 + -0.0
70,376 + +0.4
70,377 - -2.1
70,377 - +0.6
70,379 + +1.3
70,379 + -0.2
70,379 + +1.1
70,379 + -0.3
70,380 - -1.2
70,380 - +0.2
70,380 - +0.2
70,380 - +0.0
70,380 - -0.0
70,380 - -0.4
70,412 + -2.7
70,412 + -0.5
70,413 - -0.3
70,413 - +0.2
70,413 - +0.7
70,429 + +0.6
70,480 + +0.1
70,480 + +0.5
70,480 + +0.1
70,481 - +0.4
70,498 + +1.4
70,498 + +0.2
70,511 - -2.3
70,841 + Pf6N2E2_32 0.15 -0.9
70,842 - Pf6N2E2_32 0.15 +0.2
70,842 - Pf6N2E2_32 0.15 +0.3
70,866 + Pf6N2E2_32 0.17 -0.4
70,866 + Pf6N2E2_32 0.17 -1.5
70,920 - Pf6N2E2_32 0.22 -0.1
70,920 - Pf6N2E2_32 0.22 -0.3
70,986 + Pf6N2E2_32 0.28 -0.5
70,987 - Pf6N2E2_32 0.28 -1.4
70,987 - Pf6N2E2_32 0.28 +0.3
71,018 + Pf6N2E2_32 0.30 +0.1
71,018 + Pf6N2E2_32 0.30 -0.1
71,019 - Pf6N2E2_32 0.31 -1.3
71,019 - Pf6N2E2_32 0.31 -0.2
71,019 - Pf6N2E2_32 0.31 -0.2
71,019 - Pf6N2E2_32 0.31 -0.5
71,073 + Pf6N2E2_32 0.35 +0.8
71,282 + Pf6N2E2_32 0.53 +0.4
71,283 - Pf6N2E2_32 0.53 -0.4
71,834 + +0.4
71,835 - +0.3
71,865 + +0.4
71,865 + +0.4
71,866 - -1.2
71,866 - +0.5
71,866 - +0.0
71,959 + -0.6
71,959 + -0.0
71,960 - -0.5
71,960 - +0.0
71,960 - -0.2
71,960 - -0.1
71,960 - -0.3
71,964 + -0.8
72,011 - -1.2
72,011 - +0.1
72,027 + -0.1
72,028 - -1.4
72,028 - +1.8
72,028 - +0.1
72,028 - +1.1
72,028 - -0.3
72,028 - +0.3
72,028 - +0.9
72,028 - +0.6
72,031 + +0.3
72,031 + +0.3
72,031 + +0.2
72,031 + -0.1
72,032 - +0.4
72,032 - -0.5
72,255 + Pf6N2E2_33 0.26 +0.6
72,694 + Pf6N2E2_33 0.69 -0.5
72,695 - Pf6N2E2_33 0.70 -2.1

Or see this region's nucleotide sequence