Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2063 and Pf6N2E2_2064 are separated by 11 nucleotides Pf6N2E2_2064 and Pf6N2E2_2065 are separated by 77 nucleotides Pf6N2E2_2065 and Pf6N2E2_2066 are separated by 200 nucleotides Pf6N2E2_2066 and Pf6N2E2_2067 are separated by 115 nucleotides
Pf6N2E2_2063 - Predicted cobalt transporter CbtA, at 2,455,359 to 2,456,054
_2063
Pf6N2E2_2064 - Cobalt-precorrin-3b C17-methyltransferase, at 2,456,066 to 2,456,455
_2064
Pf6N2E2_2065 - Cobalt-precorrin-4 C11-methyltransferase (EC 2.1.1.133), at 2,456,533 to 2,457,279
_2065
Pf6N2E2_2066 - NfuA Fe-S protein maturation, at 2,457,480 to 2,458,064
_2066
Pf6N2E2_2067 - cis/trans isomerase, at 2,458,180 to 2,460,471
_2067
Position (kb)
2456
2457
2458 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2455.635 kb on + strand, within Pf6N2E2_2063 at 2455.635 kb on + strand, within Pf6N2E2_2063 at 2455.635 kb on + strand, within Pf6N2E2_2063 at 2455.635 kb on + strand, within Pf6N2E2_2063 at 2455.642 kb on + strand, within Pf6N2E2_2063 at 2455.693 kb on - strand, within Pf6N2E2_2063 at 2455.810 kb on + strand, within Pf6N2E2_2063 at 2456.333 kb on + strand, within Pf6N2E2_2064 at 2456.334 kb on - strand, within Pf6N2E2_2064 at 2456.334 kb on - strand, within Pf6N2E2_2064 at 2456.334 kb on - strand, within Pf6N2E2_2064 at 2456.405 kb on - strand, within Pf6N2E2_2064 at 2456.453 kb on + strand at 2456.542 kb on + strand at 2456.542 kb on + strand at 2456.543 kb on - strand at 2456.543 kb on - strand at 2456.601 kb on - strand at 2456.601 kb on - strand at 2456.626 kb on + strand, within Pf6N2E2_2065 at 2456.627 kb on - strand, within Pf6N2E2_2065 at 2456.627 kb on - strand, within Pf6N2E2_2065 at 2456.788 kb on + strand, within Pf6N2E2_2065 at 2456.789 kb on - strand, within Pf6N2E2_2065 at 2456.789 kb on - strand, within Pf6N2E2_2065 at 2457.135 kb on + strand, within Pf6N2E2_2065 at 2457.136 kb on - strand, within Pf6N2E2_2065 at 2457.245 kb on - strand at 2457.336 kb on + strand at 2457.337 kb on - strand at 2457.337 kb on - strand at 2457.337 kb on - strand at 2457.337 kb on - strand at 2457.337 kb on - strand at 2457.337 kb on - strand at 2457.478 kb on + strand at 2457.478 kb on + strand at 2457.478 kb on + strand at 2457.478 kb on + strand at 2457.478 kb on + strand at 2457.479 kb on - strand at 2457.479 kb on - strand at 2457.484 kb on + strand at 2457.484 kb on + strand at 2457.485 kb on - strand at 2457.485 kb on - strand at 2457.485 kb on - strand at 2457.485 kb on - strand at 2457.485 kb on - strand at 2457.485 kb on - strand at 2457.488 kb on - strand at 2457.488 kb on - strand at 2457.488 kb on - strand at 2458.094 kb on + strand at 2458.094 kb on + strand at 2458.194 kb on - strand at 2458.244 kb on + strand at 2458.244 kb on + strand at 2458.244 kb on + strand at 2458.244 kb on + strand at 2458.244 kb on + strand at 2458.245 kb on - strand at 2458.245 kb on - strand at 2458.245 kb on - strand at 2458.245 kb on - strand at 2458.265 kb on + strand at 2458.265 kb on + strand at 2458.265 kb on + strand at 2458.265 kb on + strand at 2458.266 kb on - strand at 2458.266 kb on - strand at 2458.266 kb on - strand at 2458.266 kb on - strand at 2458.266 kb on - strand at 2458.266 kb on - strand at 2458.268 kb on + strand at 2458.268 kb on + strand at 2458.269 kb on - strand at 2458.269 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 2,455,635 + Pf6N2E2_2063 0.40 +0.3 2,455,635 + Pf6N2E2_2063 0.40 -0.7 2,455,635 + Pf6N2E2_2063 0.40 +1.1 2,455,635 + Pf6N2E2_2063 0.40 +0.8 2,455,642 + Pf6N2E2_2063 0.41 +0.0 2,455,693 - Pf6N2E2_2063 0.48 -0.2 2,455,810 + Pf6N2E2_2063 0.65 -2.3 2,456,333 + Pf6N2E2_2064 0.68 -1.3 2,456,334 - Pf6N2E2_2064 0.69 +0.1 2,456,334 - Pf6N2E2_2064 0.69 -0.1 2,456,334 - Pf6N2E2_2064 0.69 -1.0 2,456,405 - Pf6N2E2_2064 0.87 -0.8 2,456,453 + -0.9 2,456,542 + +0.5 2,456,542 + +0.5 2,456,543 - +0.2 2,456,543 - -0.4 2,456,601 - -0.7 2,456,601 - -0.6 2,456,626 + Pf6N2E2_2065 0.12 -2.2 2,456,627 - Pf6N2E2_2065 0.13 +0.1 2,456,627 - Pf6N2E2_2065 0.13 -1.2 2,456,788 + Pf6N2E2_2065 0.34 -1.9 2,456,789 - Pf6N2E2_2065 0.34 -0.2 2,456,789 - Pf6N2E2_2065 0.34 -0.4 2,457,135 + Pf6N2E2_2065 0.81 -0.1 2,457,136 - Pf6N2E2_2065 0.81 -2.4 2,457,245 - +0.0 2,457,336 + -0.2 2,457,337 - -3.2 2,457,337 - -1.9 2,457,337 - -0.4 2,457,337 - -0.3 2,457,337 - +0.5 2,457,337 - -0.2 2,457,478 + +0.5 2,457,478 + -0.1 2,457,478 + -0.3 2,457,478 + +0.7 2,457,478 + +1.2 2,457,479 - -0.1 2,457,479 - +0.1 2,457,484 + +0.2 2,457,484 + -1.9 2,457,485 - -0.6 2,457,485 - +0.7 2,457,485 - +0.5 2,457,485 - -1.0 2,457,485 - -0.3 2,457,485 - -0.2 2,457,488 - +0.2 2,457,488 - -0.5 2,457,488 - +0.7 2,458,094 + +0.5 2,458,094 + +0.3 2,458,194 - +0.5 2,458,244 + +2.5 2,458,244 + +0.1 2,458,244 + +1.1 2,458,244 + -0.2 2,458,244 + +3.7 2,458,245 - +0.4 2,458,245 - +0.8 2,458,245 - +1.7 2,458,245 - +0.6 2,458,265 + +0.5 2,458,265 + -0.2 2,458,265 + -0.6 2,458,265 + +0.5 2,458,266 - +0.3 2,458,266 - +0.9 2,458,266 - +0.5 2,458,266 - -0.2 2,458,266 - +0.8 2,458,266 - +0.5 2,458,268 + -0.0 2,458,268 + +0.9 2,458,269 - -0.5 2,458,269 - -0.1
Or see this region's nucleotide sequence