Experiment: Nickel (II) chloride 1 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1801 and Pf6N2E2_1802 are separated by 150 nucleotides Pf6N2E2_1802 and Pf6N2E2_1803 overlap by 8 nucleotides Pf6N2E2_1803 and Pf6N2E2_1804 overlap by 4 nucleotides
Pf6N2E2_1801 - ABC-type polar amino acid transport system protein, substrate-binding protein, at 2,130,740 to 2,131,564
_1801
Pf6N2E2_1802 - polysaccharide deacetylase, at 2,131,715 to 2,132,563
_1802
Pf6N2E2_1803 - Polysaccharide deacetylase, at 2,132,556 to 2,133,443
_1803
Pf6N2E2_1804 - Immune-responsive protein 1, at 2,133,440 to 2,134,834
_1804
Position (kb)
2131
2132
2133 Strain fitness (log2 ratio)
-1
0
1 at 2130.917 kb on + strand, within Pf6N2E2_1801 at 2130.918 kb on - strand, within Pf6N2E2_1801 at 2130.918 kb on - strand, within Pf6N2E2_1801 at 2130.918 kb on - strand, within Pf6N2E2_1801 at 2131.083 kb on - strand, within Pf6N2E2_1801 at 2131.106 kb on + strand, within Pf6N2E2_1801 at 2131.107 kb on - strand, within Pf6N2E2_1801 at 2131.107 kb on - strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.370 kb on + strand, within Pf6N2E2_1801 at 2131.371 kb on - strand, within Pf6N2E2_1801 at 2131.371 kb on - strand, within Pf6N2E2_1801 at 2131.371 kb on - strand, within Pf6N2E2_1801 at 2131.371 kb on - strand, within Pf6N2E2_1801 at 2131.678 kb on + strand at 2131.678 kb on + strand at 2131.679 kb on - strand at 2131.687 kb on + strand at 2131.688 kb on - strand at 2131.688 kb on - strand at 2131.688 kb on - strand at 2131.751 kb on + strand at 2131.751 kb on + strand at 2131.752 kb on - strand at 2131.838 kb on + strand, within Pf6N2E2_1802 at 2131.945 kb on - strand, within Pf6N2E2_1802 at 2132.176 kb on + strand, within Pf6N2E2_1802 at 2132.177 kb on - strand, within Pf6N2E2_1802 at 2132.181 kb on - strand, within Pf6N2E2_1802 at 2132.214 kb on - strand, within Pf6N2E2_1802 at 2132.282 kb on + strand, within Pf6N2E2_1802 at 2132.298 kb on - strand, within Pf6N2E2_1802 at 2132.298 kb on - strand, within Pf6N2E2_1802 at 2132.403 kb on + strand, within Pf6N2E2_1802 at 2132.502 kb on + strand at 2133.114 kb on + strand, within Pf6N2E2_1803 at 2133.115 kb on - strand, within Pf6N2E2_1803 at 2133.172 kb on - strand, within Pf6N2E2_1803 at 2133.234 kb on + strand, within Pf6N2E2_1803 at 2133.235 kb on - strand, within Pf6N2E2_1803 at 2133.440 kb on - strand at 2133.541 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM remove 2,130,917 + Pf6N2E2_1801 0.21 -0.4 2,130,918 - Pf6N2E2_1801 0.22 +0.3 2,130,918 - Pf6N2E2_1801 0.22 -0.2 2,130,918 - Pf6N2E2_1801 0.22 +0.7 2,131,083 - Pf6N2E2_1801 0.42 +0.4 2,131,106 + Pf6N2E2_1801 0.44 +0.8 2,131,107 - Pf6N2E2_1801 0.44 +0.2 2,131,107 - Pf6N2E2_1801 0.44 -1.2 2,131,370 + Pf6N2E2_1801 0.76 +0.1 2,131,370 + Pf6N2E2_1801 0.76 +0.3 2,131,370 + Pf6N2E2_1801 0.76 -0.1 2,131,370 + Pf6N2E2_1801 0.76 +1.3 2,131,370 + Pf6N2E2_1801 0.76 +1.1 2,131,370 + Pf6N2E2_1801 0.76 +0.4 2,131,371 - Pf6N2E2_1801 0.76 -0.7 2,131,371 - Pf6N2E2_1801 0.76 +0.2 2,131,371 - Pf6N2E2_1801 0.76 +0.6 2,131,371 - Pf6N2E2_1801 0.76 +0.3 2,131,678 + -0.0 2,131,678 + +0.4 2,131,679 - -1.0 2,131,687 + -0.1 2,131,688 - +0.4 2,131,688 - -0.1 2,131,688 - -0.4 2,131,751 + +0.4 2,131,751 + -0.4 2,131,752 - +0.1 2,131,838 + Pf6N2E2_1802 0.14 +0.0 2,131,945 - Pf6N2E2_1802 0.27 -0.5 2,132,176 + Pf6N2E2_1802 0.54 -0.9 2,132,177 - Pf6N2E2_1802 0.54 -0.1 2,132,181 - Pf6N2E2_1802 0.55 -0.7 2,132,214 - Pf6N2E2_1802 0.59 -0.9 2,132,282 + Pf6N2E2_1802 0.67 -0.8 2,132,298 - Pf6N2E2_1802 0.69 -0.6 2,132,298 - Pf6N2E2_1802 0.69 -0.6 2,132,403 + Pf6N2E2_1802 0.81 -0.1 2,132,502 + +0.4 2,133,114 + Pf6N2E2_1803 0.63 +0.5 2,133,115 - Pf6N2E2_1803 0.63 +0.3 2,133,172 - Pf6N2E2_1803 0.69 -0.5 2,133,234 + Pf6N2E2_1803 0.76 -0.2 2,133,235 - Pf6N2E2_1803 0.76 +1.3 2,133,440 - +0.8 2,133,541 + -0.1
Or see this region's nucleotide sequence