Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1285

Experiment: Nickel (II) chloride 1 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1283 and Pf6N2E2_1284 are separated by 35 nucleotidesPf6N2E2_1284 and Pf6N2E2_1285 are separated by 10 nucleotidesPf6N2E2_1285 and Pf6N2E2_1286 are separated by 17 nucleotides Pf6N2E2_1283 - Ferric siderophore transport system, periplasmic binding protein TonB, at 1,513,207 to 1,513,878 _1283 Pf6N2E2_1284 - Biopolymer transport protein ExbD/TolR, at 1,513,914 to 1,514,339 _1284 Pf6N2E2_1285 - MotA/TolQ/ExbB proton channel family protein, at 1,514,350 to 1,516,149 _1285 Pf6N2E2_1286 - Hemolysin activation/secretion protein associated with VreARI signalling system, at 1,516,167 to 1,517,771 _1286 Position (kb) 1514 1515 1516 1517Strain fitness (log2 ratio) -2 -1 0 1at 1513.472 kb on + strand, within Pf6N2E2_1283at 1513.472 kb on + strand, within Pf6N2E2_1283at 1513.473 kb on - strand, within Pf6N2E2_1283at 1513.473 kb on - strand, within Pf6N2E2_1283at 1514.002 kb on + strand, within Pf6N2E2_1284at 1514.328 kb on + strandat 1514.508 kb on - strandat 1514.508 kb on - strandat 1514.634 kb on + strand, within Pf6N2E2_1285at 1514.635 kb on - strand, within Pf6N2E2_1285at 1514.635 kb on - strand, within Pf6N2E2_1285at 1514.635 kb on - strand, within Pf6N2E2_1285at 1514.635 kb on - strand, within Pf6N2E2_1285at 1514.843 kb on + strand, within Pf6N2E2_1285at 1514.843 kb on + strand, within Pf6N2E2_1285at 1514.844 kb on - strand, within Pf6N2E2_1285at 1514.844 kb on - strand, within Pf6N2E2_1285at 1514.845 kb on + strand, within Pf6N2E2_1285at 1514.846 kb on - strand, within Pf6N2E2_1285at 1515.122 kb on + strand, within Pf6N2E2_1285at 1515.329 kb on + strand, within Pf6N2E2_1285at 1515.531 kb on + strand, within Pf6N2E2_1285at 1515.719 kb on + strand, within Pf6N2E2_1285at 1515.720 kb on - strand, within Pf6N2E2_1285at 1515.720 kb on - strand, within Pf6N2E2_1285at 1515.791 kb on + strand, within Pf6N2E2_1285at 1515.791 kb on + strand, within Pf6N2E2_1285at 1515.791 kb on + strand, within Pf6N2E2_1285at 1515.791 kb on + strand, within Pf6N2E2_1285at 1515.791 kb on + strand, within Pf6N2E2_1285at 1515.792 kb on - strand, within Pf6N2E2_1285at 1516.123 kb on - strandat 1516.138 kb on + strandat 1516.138 kb on + strandat 1516.379 kb on - strand, within Pf6N2E2_1286at 1516.399 kb on + strand, within Pf6N2E2_1286at 1516.399 kb on + strand, within Pf6N2E2_1286at 1516.606 kb on + strand, within Pf6N2E2_1286at 1516.768 kb on + strand, within Pf6N2E2_1286at 1516.789 kb on + strand, within Pf6N2E2_1286at 1516.903 kb on + strand, within Pf6N2E2_1286at 1516.921 kb on + strand, within Pf6N2E2_1286at 1516.922 kb on - strand, within Pf6N2E2_1286at 1516.922 kb on - strand, within Pf6N2E2_1286at 1517.122 kb on + strand, within Pf6N2E2_1286at 1517.122 kb on + strand, within Pf6N2E2_1286

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Per-strain Table

Position Strand Gene LocusTag Fraction Nickel (II) chloride 1 mM
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1,513,472 + Pf6N2E2_1283 0.39 +0.8
1,513,472 + Pf6N2E2_1283 0.39 +0.8
1,513,473 - Pf6N2E2_1283 0.40 -1.6
1,513,473 - Pf6N2E2_1283 0.40 +0.7
1,514,002 + Pf6N2E2_1284 0.21 +1.0
1,514,328 + -0.6
1,514,508 - +0.8
1,514,508 - -0.0
1,514,634 + Pf6N2E2_1285 0.16 -1.0
1,514,635 - Pf6N2E2_1285 0.16 +0.1
1,514,635 - Pf6N2E2_1285 0.16 -0.9
1,514,635 - Pf6N2E2_1285 0.16 -0.0
1,514,635 - Pf6N2E2_1285 0.16 -0.5
1,514,843 + Pf6N2E2_1285 0.27 -0.3
1,514,843 + Pf6N2E2_1285 0.27 -0.3
1,514,844 - Pf6N2E2_1285 0.27 +0.4
1,514,844 - Pf6N2E2_1285 0.27 -0.3
1,514,845 + Pf6N2E2_1285 0.28 +0.0
1,514,846 - Pf6N2E2_1285 0.28 -0.3
1,515,122 + Pf6N2E2_1285 0.43 -0.3
1,515,329 + Pf6N2E2_1285 0.54 +0.6
1,515,531 + Pf6N2E2_1285 0.66 -0.1
1,515,719 + Pf6N2E2_1285 0.76 -1.1
1,515,720 - Pf6N2E2_1285 0.76 -2.2
1,515,720 - Pf6N2E2_1285 0.76 -0.0
1,515,791 + Pf6N2E2_1285 0.80 +0.7
1,515,791 + Pf6N2E2_1285 0.80 +0.6
1,515,791 + Pf6N2E2_1285 0.80 +0.0
1,515,791 + Pf6N2E2_1285 0.80 -0.2
1,515,791 + Pf6N2E2_1285 0.80 -0.3
1,515,792 - Pf6N2E2_1285 0.80 -0.6
1,516,123 - +1.1
1,516,138 + +1.1
1,516,138 + -0.7
1,516,379 - Pf6N2E2_1286 0.13 +0.4
1,516,399 + Pf6N2E2_1286 0.14 +0.5
1,516,399 + Pf6N2E2_1286 0.14 -0.5
1,516,606 + Pf6N2E2_1286 0.27 -2.2
1,516,768 + Pf6N2E2_1286 0.37 +0.8
1,516,789 + Pf6N2E2_1286 0.39 -0.6
1,516,903 + Pf6N2E2_1286 0.46 +0.1
1,516,921 + Pf6N2E2_1286 0.47 +0.3
1,516,922 - Pf6N2E2_1286 0.47 -0.3
1,516,922 - Pf6N2E2_1286 0.47 +0.9
1,517,122 + Pf6N2E2_1286 0.60 +0.6
1,517,122 + Pf6N2E2_1286 0.60 -0.2

Or see this region's nucleotide sequence