Experiment: pyruvate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3212 and Pf1N1B4_3213 overlap by 7 nucleotides Pf1N1B4_3213 and Pf1N1B4_3214 are separated by 369 nucleotides Pf1N1B4_3214 and Pf1N1B4_3215 are separated by 0 nucleotides Pf1N1B4_3215 and Pf1N1B4_3216 overlap by 4 nucleotides
Pf1N1B4_3212 - hypothetical protein, at 3,594,481 to 3,595,173
_3212
Pf1N1B4_3213 - hypothetical protein, at 3,595,167 to 3,595,376
_3213
Pf1N1B4_3214 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 3,595,746 to 3,596,447
_3214
Pf1N1B4_3215 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 3,596,448 to 3,597,215
_3215
Pf1N1B4_3216 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 3,597,212 to 3,598,468
_3216
Position (kb)
3595
3596
3597 Strain fitness (log2 ratio)
-1
0
1
2 at 3594.785 kb on + strand, within Pf1N1B4_3212 at 3594.786 kb on - strand, within Pf1N1B4_3212 at 3594.870 kb on - strand, within Pf1N1B4_3212 at 3594.870 kb on - strand, within Pf1N1B4_3212 at 3595.148 kb on - strand at 3595.227 kb on - strand, within Pf1N1B4_3213 at 3595.433 kb on - strand at 3595.437 kb on - strand at 3595.504 kb on - strand at 3595.596 kb on - strand at 3595.650 kb on + strand at 3595.650 kb on + strand at 3595.650 kb on + strand at 3595.650 kb on + strand at 3595.662 kb on - strand at 3595.700 kb on + strand at 3595.759 kb on + strand at 3595.837 kb on + strand, within Pf1N1B4_3214 at 3595.837 kb on + strand at 3595.837 kb on + strand, within Pf1N1B4_3214 at 3595.838 kb on - strand, within Pf1N1B4_3214 at 3595.838 kb on - strand, within Pf1N1B4_3214 at 3596.122 kb on + strand, within Pf1N1B4_3214 at 3596.222 kb on + strand at 3596.222 kb on + strand, within Pf1N1B4_3214 at 3596.223 kb on - strand, within Pf1N1B4_3214 at 3596.223 kb on - strand, within Pf1N1B4_3214 at 3596.223 kb on - strand, within Pf1N1B4_3214 at 3596.223 kb on - strand at 3596.269 kb on + strand, within Pf1N1B4_3214 at 3596.269 kb on + strand, within Pf1N1B4_3214 at 3596.270 kb on - strand, within Pf1N1B4_3214 at 3596.416 kb on + strand at 3596.416 kb on + strand at 3596.416 kb on + strand at 3596.417 kb on - strand at 3596.417 kb on - strand at 3596.417 kb on - strand at 3596.449 kb on + strand at 3596.449 kb on + strand at 3596.449 kb on + strand at 3596.449 kb on + strand at 3596.450 kb on - strand at 3596.450 kb on - strand at 3596.450 kb on - strand at 3596.450 kb on - strand at 3596.464 kb on + strand at 3596.464 kb on + strand at 3596.810 kb on - strand, within Pf1N1B4_3215 at 3597.368 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction pyruvate (C) remove 3,594,785 + Pf1N1B4_3212 0.44 +0.2 3,594,786 - Pf1N1B4_3212 0.44 -0.6 3,594,870 - Pf1N1B4_3212 0.56 -0.1 3,594,870 - Pf1N1B4_3212 0.56 +0.9 3,595,148 - +0.7 3,595,227 - Pf1N1B4_3213 0.29 +0.1 3,595,433 - -0.1 3,595,437 - -0.5 3,595,504 - -0.6 3,595,596 - +0.7 3,595,650 + -0.9 3,595,650 + +0.8 3,595,650 + +0.3 3,595,650 + +2.4 3,595,662 - +0.2 3,595,700 + -0.2 3,595,759 + -0.8 3,595,837 + Pf1N1B4_3214 0.13 -1.4 3,595,837 + +0.5 3,595,837 + Pf1N1B4_3214 0.13 -0.4 3,595,838 - Pf1N1B4_3214 0.13 -0.4 3,595,838 - Pf1N1B4_3214 0.13 -0.8 3,596,122 + Pf1N1B4_3214 0.54 -0.9 3,596,222 + +0.6 3,596,222 + Pf1N1B4_3214 0.68 -1.2 3,596,223 - Pf1N1B4_3214 0.68 -1.1 3,596,223 - Pf1N1B4_3214 0.68 -1.1 3,596,223 - Pf1N1B4_3214 0.68 +1.2 3,596,223 - -0.4 3,596,269 + Pf1N1B4_3214 0.75 +0.7 3,596,269 + Pf1N1B4_3214 0.75 -1.6 3,596,270 - Pf1N1B4_3214 0.75 +1.5 3,596,416 + +0.5 3,596,416 + -0.4 3,596,416 + +0.2 3,596,417 - -0.9 3,596,417 - +0.5 3,596,417 - +0.2 3,596,449 + +1.9 3,596,449 + -1.2 3,596,449 + +0.6 3,596,449 + -0.4 3,596,450 - +0.2 3,596,450 - -0.7 3,596,450 - -0.4 3,596,450 - +0.9 3,596,464 + +1.8 3,596,464 + -0.1 3,596,810 - Pf1N1B4_3215 0.47 -0.1 3,597,368 + +0.2
Or see this region's nucleotide sequence