Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS08030

Experiment: Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS08020 and MMJJ_RS08025 are separated by 72 nucleotidesMMJJ_RS08025 and MMJJ_RS08030 are separated by 94 nucleotidesMMJJ_RS08030 and MMJJ_RS08035 are separated by 13 nucleotidesMMJJ_RS08035 and MMJJ_RS08040 are separated by 81 nucleotides MMJJ_RS08020: MMJJ_RS08020 - nucleotidyltransferase domain-containing protein, at 1,468,595 to 1,469,653 _RS08020 MMJJ_RS08025: MMJJ_RS08025 - hypothetical protein, at 1,469,726 to 1,470,028 _RS08025 MMJJ_RS08030: MMJJ_RS08030 - hypothetical protein, at 1,470,123 to 1,470,770 _RS08030 MMJJ_RS08035: MMJJ_RS08035 - cobalt-precorrin-7 (C(5))-methyltransferase, at 1,470,784 to 1,471,443 _RS08035 MMJJ_RS08040: MMJJ_RS08040 - NAD(P)/FAD-dependent oxidoreductase, at 1,471,525 to 1,472,682 _RS08040 Position (kb) 1470 1471Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 1469.192 kb on - strand, within MMJJ_RS08020at 1469.257 kb on - strand, within MMJJ_RS08020at 1469.258 kb on + strand, within MMJJ_RS08020at 1469.397 kb on - strand, within MMJJ_RS08020at 1469.447 kb on + strand, within MMJJ_RS08020at 1469.449 kb on - strand, within MMJJ_RS08020at 1469.484 kb on + strand, within MMJJ_RS08020at 1469.489 kb on - strand, within MMJJ_RS08020at 1469.549 kb on - strandat 1469.549 kb on - strandat 1469.550 kb on + strandat 1469.642 kb on + strandat 1469.682 kb on + strandat 1469.689 kb on - strandat 1469.689 kb on - strandat 1469.690 kb on + strandat 1469.694 kb on - strandat 1469.695 kb on + strandat 1469.696 kb on - strandat 1469.696 kb on - strandat 1469.696 kb on - strandat 1469.697 kb on + strandat 1469.702 kb on + strandat 1469.706 kb on - strandat 1469.706 kb on - strandat 1469.706 kb on - strandat 1469.706 kb on - strandat 1469.707 kb on + strandat 1469.710 kb on - strandat 1469.710 kb on - strandat 1469.710 kb on - strandat 1469.710 kb on - strandat 1469.711 kb on + strandat 1469.711 kb on + strandat 1469.711 kb on + strandat 1469.711 kb on + strandat 1469.711 kb on + strandat 1469.711 kb on + strandat 1469.712 kb on - strandat 1469.713 kb on + strandat 1469.713 kb on + strandat 1469.713 kb on + strandat 1469.835 kb on + strand, within MMJJ_RS08025at 1469.835 kb on + strand, within MMJJ_RS08025at 1469.912 kb on - strand, within MMJJ_RS08025at 1469.913 kb on + strand, within MMJJ_RS08025at 1469.974 kb on - strand, within MMJJ_RS08025at 1469.975 kb on + strand, within MMJJ_RS08025at 1469.987 kb on + strand, within MMJJ_RS08025at 1470.020 kb on - strandat 1470.033 kb on - strandat 1470.055 kb on + strandat 1470.060 kb on - strandat 1470.063 kb on - strandat 1470.064 kb on + strandat 1470.068 kb on + strandat 1470.161 kb on - strandat 1470.164 kb on - strandat 1470.243 kb on - strand, within MMJJ_RS08030at 1470.362 kb on + strand, within MMJJ_RS08030at 1470.391 kb on - strand, within MMJJ_RS08030at 1470.409 kb on - strand, within MMJJ_RS08030at 1470.409 kb on - strand, within MMJJ_RS08030at 1470.472 kb on + strand, within MMJJ_RS08030at 1470.473 kb on - strand, within MMJJ_RS08030at 1470.539 kb on - strand, within MMJJ_RS08030at 1470.540 kb on + strand, within MMJJ_RS08030at 1470.545 kb on - strand, within MMJJ_RS08030at 1470.570 kb on - strand, within MMJJ_RS08030at 1470.571 kb on + strand, within MMJJ_RS08030at 1470.581 kb on + strand, within MMJJ_RS08030at 1470.608 kb on - strand, within MMJJ_RS08030at 1470.609 kb on + strand, within MMJJ_RS08030at 1470.614 kb on - strand, within MMJJ_RS08030at 1470.615 kb on + strand, within MMJJ_RS08030at 1470.618 kb on - strand, within MMJJ_RS08030at 1470.618 kb on - strand, within MMJJ_RS08030at 1470.619 kb on + strand, within MMJJ_RS08030at 1470.625 kb on + strand, within MMJJ_RS08030at 1470.630 kb on + strand, within MMJJ_RS08030at 1470.630 kb on + strand, within MMJJ_RS08030at 1470.635 kb on - strand, within MMJJ_RS08030at 1470.643 kb on - strand, within MMJJ_RS08030at 1470.643 kb on - strand, within MMJJ_RS08030at 1470.644 kb on + strand, within MMJJ_RS08030at 1470.648 kb on + strand, within MMJJ_RS08030at 1470.665 kb on - strand, within MMJJ_RS08030at 1470.723 kb on - strandat 1470.726 kb on - strandat 1470.726 kb on - strandat 1470.726 kb on - strandat 1470.727 kb on + strandat 1470.732 kb on - strandat 1470.750 kb on - strandat 1470.775 kb on + strandat 1470.791 kb on + strandat 1470.791 kb on + strandat 1470.791 kb on + strandat 1470.792 kb on - strandat 1470.793 kb on + strandat 1470.809 kb on - strandat 1471.027 kb on - strand, within MMJJ_RS08035at 1471.027 kb on - strand, within MMJJ_RS08035at 1471.028 kb on + strand, within MMJJ_RS08035at 1471.042 kb on + strand, within MMJJ_RS08035at 1471.043 kb on - strand, within MMJJ_RS08035at 1471.146 kb on - strand, within MMJJ_RS08035at 1471.146 kb on - strandat 1471.146 kb on - strand, within MMJJ_RS08035at 1471.146 kb on - strand, within MMJJ_RS08035at 1471.154 kb on + strand, within MMJJ_RS08035at 1471.242 kb on + strand, within MMJJ_RS08035at 1471.262 kb on + strand, within MMJJ_RS08035at 1471.365 kb on - strand, within MMJJ_RS08035at 1471.370 kb on - strand, within MMJJ_RS08035at 1471.370 kb on - strand, within MMJJ_RS08035at 1471.371 kb on + strand, within MMJJ_RS08035at 1471.371 kb on + strand, within MMJJ_RS08035at 1471.401 kb on - strandat 1471.403 kb on - strandat 1471.403 kb on - strandat 1471.404 kb on + strandat 1471.413 kb on - strandat 1471.454 kb on - strandat 1471.455 kb on + strandat 1471.455 kb on + strandat 1471.455 kb on + strandat 1471.460 kb on - strandat 1471.461 kb on + strandat 1471.472 kb on - strandat 1471.473 kb on + strandat 1471.473 kb on + strandat 1471.473 kb on + strandat 1471.476 kb on + strandat 1471.476 kb on + strandat 1471.477 kb on - strandat 1471.477 kb on - strandat 1471.477 kb on - strandat 1471.478 kb on + strandat 1471.478 kb on + strandat 1471.485 kb on + strandat 1471.492 kb on + strandat 1471.492 kb on + strandat 1471.521 kb on - strandat 1471.522 kb on + strandat 1471.540 kb on - strandat 1471.541 kb on + strandat 1471.541 kb on + strandat 1471.541 kb on + strandat 1471.550 kb on - strandat 1471.550 kb on - strandat 1471.578 kb on - strandat 1471.578 kb on - strandat 1471.579 kb on + strandat 1471.593 kb on - strandat 1471.602 kb on - strandat 1471.667 kb on - strand, within MMJJ_RS08040at 1471.668 kb on + strand, within MMJJ_RS08040at 1471.716 kb on - strand, within MMJJ_RS08040at 1471.723 kb on + strand, within MMJJ_RS08040at 1471.735 kb on + strand, within MMJJ_RS08040

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Per-strain Table

Position Strand Gene LocusTag Fraction Formate McCas, 2.5g/L bicarb, 200 mM MOPs, 20 psi N2CO2, SO4 FREE
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1,469,192 - MMJJ_RS08020 0.56 -4.7
1,469,257 - MMJJ_RS08020 0.63 -4.4
1,469,258 + MMJJ_RS08020 0.63 -3.8
1,469,397 - MMJJ_RS08020 0.76 -1.0
1,469,447 + MMJJ_RS08020 0.80 -0.6
1,469,449 - MMJJ_RS08020 0.81 -3.3
1,469,484 + MMJJ_RS08020 0.84 -5.7
1,469,489 - MMJJ_RS08020 0.84 -4.0
1,469,549 - -5.1
1,469,549 - -4.5
1,469,550 + -4.7
1,469,642 + -2.8
1,469,682 + -0.3
1,469,689 - -0.5
1,469,689 - +0.3
1,469,690 + -0.7
1,469,694 - +1.5
1,469,695 + -1.4
1,469,696 - +3.2
1,469,696 - +0.4
1,469,696 - +2.0
1,469,697 + -1.1
1,469,702 + +0.5
1,469,706 - +0.9
1,469,706 - +0.9
1,469,706 - -1.0
1,469,706 - -0.7
1,469,707 + -2.3
1,469,710 - +0.1
1,469,710 - +0.2
1,469,710 - +0.2
1,469,710 - -0.3
1,469,711 + -1.1
1,469,711 + -0.4
1,469,711 + +0.6
1,469,711 + -0.8
1,469,711 + -0.7
1,469,711 + +0.1
1,469,712 - -0.2
1,469,713 + -0.3
1,469,713 + -1.1
1,469,713 + +1.0
1,469,835 + MMJJ_RS08025 0.36 -1.0
1,469,835 + MMJJ_RS08025 0.36 +2.4
1,469,912 - MMJJ_RS08025 0.61 +0.2
1,469,913 + MMJJ_RS08025 0.62 -0.3
1,469,974 - MMJJ_RS08025 0.82 -1.6
1,469,975 + MMJJ_RS08025 0.82 -0.0
1,469,987 + MMJJ_RS08025 0.86 +0.3
1,470,020 - -1.0
1,470,033 - -0.4
1,470,055 + -0.1
1,470,060 - -1.3
1,470,063 - +0.4
1,470,064 + +0.4
1,470,068 + -0.0
1,470,161 - -0.2
1,470,164 - +1.0
1,470,243 - MMJJ_RS08030 0.19 -0.0
1,470,362 + MMJJ_RS08030 0.37 -1.0
1,470,391 - MMJJ_RS08030 0.41 +1.5
1,470,409 - MMJJ_RS08030 0.44 -0.1
1,470,409 - MMJJ_RS08030 0.44 -0.3
1,470,472 + MMJJ_RS08030 0.54 -0.1
1,470,473 - MMJJ_RS08030 0.54 -1.6
1,470,539 - MMJJ_RS08030 0.64 -0.1
1,470,540 + MMJJ_RS08030 0.64 +0.5
1,470,545 - MMJJ_RS08030 0.65 +0.2
1,470,570 - MMJJ_RS08030 0.69 +0.5
1,470,571 + MMJJ_RS08030 0.69 -0.2
1,470,581 + MMJJ_RS08030 0.71 -0.3
1,470,608 - MMJJ_RS08030 0.75 -0.3
1,470,609 + MMJJ_RS08030 0.75 +0.1
1,470,614 - MMJJ_RS08030 0.76 +0.5
1,470,615 + MMJJ_RS08030 0.76 -1.6
1,470,618 - MMJJ_RS08030 0.76 +0.4
1,470,618 - MMJJ_RS08030 0.76 -0.1
1,470,619 + MMJJ_RS08030 0.77 -0.1
1,470,625 + MMJJ_RS08030 0.77 +0.8
1,470,630 + MMJJ_RS08030 0.78 -0.6
1,470,630 + MMJJ_RS08030 0.78 -0.3
1,470,635 - MMJJ_RS08030 0.79 -0.2
1,470,643 - MMJJ_RS08030 0.80 +0.1
1,470,643 - MMJJ_RS08030 0.80 +0.2
1,470,644 + MMJJ_RS08030 0.80 -0.1
1,470,648 + MMJJ_RS08030 0.81 -0.6
1,470,665 - MMJJ_RS08030 0.84 +1.1
1,470,723 - +0.2
1,470,726 - -0.4
1,470,726 - -0.2
1,470,726 - +0.6
1,470,727 + -0.1
1,470,732 - -0.9
1,470,750 - -0.9
1,470,775 + -0.6
1,470,791 + +1.0
1,470,791 + -1.8
1,470,791 + -0.6
1,470,792 - +0.7
1,470,793 + +0.2
1,470,809 - -2.3
1,471,027 - MMJJ_RS08035 0.37 -2.2
1,471,027 - MMJJ_RS08035 0.37 -3.2
1,471,028 + MMJJ_RS08035 0.37 -1.8
1,471,042 + MMJJ_RS08035 0.39 -1.6
1,471,043 - MMJJ_RS08035 0.39 -3.9
1,471,146 - MMJJ_RS08035 0.55 -2.3
1,471,146 - -0.0
1,471,146 - MMJJ_RS08035 0.55 -0.0
1,471,146 - MMJJ_RS08035 0.55 -2.0
1,471,154 + MMJJ_RS08035 0.56 -2.8
1,471,242 + MMJJ_RS08035 0.69 -3.9
1,471,262 + MMJJ_RS08035 0.72 -2.9
1,471,365 - MMJJ_RS08035 0.88 -2.6
1,471,370 - MMJJ_RS08035 0.89 -2.8
1,471,370 - MMJJ_RS08035 0.89 -4.7
1,471,371 + MMJJ_RS08035 0.89 -2.8
1,471,371 + MMJJ_RS08035 0.89 -2.8
1,471,401 - -4.6
1,471,403 - -1.0
1,471,403 - -1.3
1,471,404 + -2.6
1,471,413 - -4.0
1,471,454 - -0.3
1,471,455 + -0.9
1,471,455 + -0.5
1,471,455 + +0.3
1,471,460 - +0.2
1,471,461 + +0.8
1,471,472 - +2.0
1,471,473 + -1.0
1,471,473 + -0.9
1,471,473 + +0.6
1,471,476 + +1.3
1,471,476 + -0.0
1,471,477 - -1.1
1,471,477 - +1.0
1,471,477 - -0.6
1,471,478 + +0.7
1,471,478 + +2.0
1,471,485 + +1.4
1,471,492 + +1.2
1,471,492 + +2.6
1,471,521 - +0.9
1,471,522 + +1.6
1,471,540 - +1.0
1,471,541 + +0.9
1,471,541 + +1.6
1,471,541 + +0.5
1,471,550 - +0.6
1,471,550 - +1.3
1,471,578 - +0.3
1,471,578 - +1.9
1,471,579 + +0.1
1,471,593 - +0.2
1,471,602 - -0.0
1,471,667 - MMJJ_RS08040 0.12 +0.7
1,471,668 + MMJJ_RS08040 0.12 +0.8
1,471,716 - MMJJ_RS08040 0.16 +0.7
1,471,723 + MMJJ_RS08040 0.17 +0.8
1,471,735 + MMJJ_RS08040 0.18 -0.0

Or see this region's nucleotide sequence