Experiment: R2A with Manganese (II) chloride tetrahydrate 1.25 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK54_RS03130 and LRK54_RS03135 are separated by 96 nucleotides LRK54_RS03135 and LRK54_RS03140 are separated by 65 nucleotides LRK54_RS03140 and LRK54_RS03145 are separated by 75 nucleotides
LRK54_RS03130: LRK54_RS03130 - tRNA guanosine(34) transglycosylase Tgt, at 674,692 to 675,819
_RS03130
LRK54_RS03135: LRK54_RS03135 - tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA, at 675,916 to 676,947
_RS03135
LRK54_RS03140: LRK54_RS03140 - response regulator transcription factor, at 677,013 to 677,615
_RS03140
LRK54_RS03145: LRK54_RS03145 - sensor histidine kinase, at 677,691 to 678,920
_RS03145
Position (kb) 675
676
677 Strain fitness (log2 ratio) -2
-1
0
1
2 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.939 kb on + strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.940 kb on - strand, within LRK54_RS03130 at 674.965 kb on - strand, within LRK54_RS03130 at 675.006 kb on + strand, within LRK54_RS03130 at 675.006 kb on + strand, within LRK54_RS03130 at 675.007 kb on - strand, within LRK54_RS03130 at 675.007 kb on - strand, within LRK54_RS03130 at 675.086 kb on + strand, within LRK54_RS03130 at 675.182 kb on + strand, within LRK54_RS03130 at 675.183 kb on - strand, within LRK54_RS03130 at 675.239 kb on + strand, within LRK54_RS03130 at 675.239 kb on + strand, within LRK54_RS03130 at 675.240 kb on - strand, within LRK54_RS03130 at 675.240 kb on - strand, within LRK54_RS03130 at 675.369 kb on - strand, within LRK54_RS03130 at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.707 kb on + strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.708 kb on - strand at 675.953 kb on + strand at 675.953 kb on + strand at 675.953 kb on + strand at 675.953 kb on + strand at 675.953 kb on + strand at 675.953 kb on + strand at 675.954 kb on - strand at 675.954 kb on - strand at 675.954 kb on - strand at 675.954 kb on - strand at 675.954 kb on - strand at 675.954 kb on - strand at 675.992 kb on + strand at 675.992 kb on + strand at 675.992 kb on + strand at 675.992 kb on + strand at 675.993 kb on - strand at 675.993 kb on - strand at 675.993 kb on - strand at 675.993 kb on - strand at 675.993 kb on - strand at 675.993 kb on - strand at 675.993 kb on - strand at 676.350 kb on - strand, within LRK54_RS03135 at 676.350 kb on - strand, within LRK54_RS03135 at 676.350 kb on - strand, within LRK54_RS03135 at 676.417 kb on - strand, within LRK54_RS03135 at 676.460 kb on + strand, within LRK54_RS03135 at 676.499 kb on + strand, within LRK54_RS03135 at 676.499 kb on + strand, within LRK54_RS03135 at 676.499 kb on + strand, within LRK54_RS03135 at 676.499 kb on + strand, within LRK54_RS03135 at 676.499 kb on + strand, within LRK54_RS03135 at 676.499 kb on + strand, within LRK54_RS03135 at 676.499 kb on + strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.500 kb on - strand, within LRK54_RS03135 at 676.742 kb on + strand, within LRK54_RS03135 at 676.742 kb on + strand, within LRK54_RS03135 at 676.742 kb on + strand, within LRK54_RS03135 at 676.742 kb on + strand, within LRK54_RS03135 at 676.742 kb on + strand, within LRK54_RS03135 at 676.742 kb on + strand, within LRK54_RS03135 at 676.743 kb on - strand, within LRK54_RS03135 at 676.952 kb on + strand at 676.953 kb on - strand at 676.953 kb on - strand at 676.953 kb on - strand at 676.953 kb on - strand at 676.953 kb on - strand at 676.953 kb on - strand at 676.953 kb on - strand at 676.953 kb on - strand at 676.953 kb on - strand at 676.953 kb on - strand at 676.968 kb on + strand at 676.968 kb on + strand at 676.968 kb on + strand at 676.968 kb on + strand at 676.968 kb on + strand at 676.968 kb on + strand at 676.968 kb on + strand at 676.968 kb on + strand at 676.968 kb on + strand at 676.968 kb on + strand at 676.968 kb on + strand at 676.968 kb on + strand at 676.968 kb on + strand at 676.968 kb on + strand at 676.969 kb on - strand at 676.969 kb on - strand at 676.969 kb on - strand at 676.969 kb on - strand at 676.969 kb on - strand at 676.969 kb on - strand at 676.969 kb on - strand at 676.969 kb on - strand at 676.969 kb on - strand at 676.969 kb on - strand at 676.969 kb on - strand at 676.969 kb on - strand at 676.969 kb on - strand at 676.980 kb on + strand at 676.980 kb on + strand at 676.981 kb on - strand at 676.981 kb on - strand at 676.981 kb on - strand at 677.014 kb on + strand at 677.014 kb on + strand at 677.014 kb on + strand at 677.014 kb on + strand at 677.014 kb on + strand at 677.014 kb on + strand at 677.014 kb on + strand at 677.014 kb on + strand at 677.014 kb on + strand at 677.014 kb on + strand at 677.015 kb on - strand at 677.015 kb on - strand at 677.015 kb on - strand at 677.015 kb on - strand at 677.017 kb on - strand at 677.017 kb on - strand at 677.017 kb on - strand at 677.017 kb on - strand at 677.017 kb on - strand at 677.044 kb on + strand at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.095 kb on + strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.096 kb on - strand, within LRK54_RS03140 at 677.314 kb on + strand, within LRK54_RS03140 at 677.314 kb on + strand, within LRK54_RS03140 at 677.315 kb on - strand, within LRK54_RS03140 at 677.315 kb on - strand, within LRK54_RS03140
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Manganese (II) chloride tetrahydrate 1.25 mM remove 674,939 + LRK54_RS03130 0.22 -0.2 674,939 + LRK54_RS03130 0.22 +0.2 674,939 + LRK54_RS03130 0.22 -0.3 674,939 + LRK54_RS03130 0.22 -0.2 674,939 + LRK54_RS03130 0.22 -0.8 674,939 + LRK54_RS03130 0.22 -1.5 674,939 + LRK54_RS03130 0.22 -2.4 674,939 + LRK54_RS03130 0.22 +0.2 674,939 + LRK54_RS03130 0.22 -1.9 674,940 - LRK54_RS03130 0.22 -1.8 674,940 - LRK54_RS03130 0.22 -2.2 674,940 - LRK54_RS03130 0.22 -0.7 674,940 - LRK54_RS03130 0.22 -2.0 674,940 - LRK54_RS03130 0.22 -0.8 674,940 - LRK54_RS03130 0.22 -1.0 674,940 - LRK54_RS03130 0.22 -1.2 674,940 - LRK54_RS03130 0.22 -0.8 674,965 - LRK54_RS03130 0.24 +0.1 675,006 + LRK54_RS03130 0.28 -0.9 675,006 + LRK54_RS03130 0.28 +0.2 675,007 - LRK54_RS03130 0.28 -2.4 675,007 - LRK54_RS03130 0.28 -0.9 675,086 + LRK54_RS03130 0.35 -0.1 675,182 + LRK54_RS03130 0.43 -1.0 675,183 - LRK54_RS03130 0.44 +1.6 675,239 + LRK54_RS03130 0.48 -1.6 675,239 + LRK54_RS03130 0.48 -0.9 675,240 - LRK54_RS03130 0.49 -2.2 675,240 - LRK54_RS03130 0.49 -0.1 675,369 - LRK54_RS03130 0.60 -1.4 675,707 + -0.7 675,707 + -0.5 675,707 + -0.4 675,707 + -0.7 675,707 + -0.2 675,707 + +0.5 675,707 + -0.5 675,707 + -0.9 675,707 + +0.5 675,707 + +0.2 675,707 + -0.8 675,708 - -1.3 675,708 - -0.3 675,708 - -1.2 675,708 - -0.1 675,708 - +0.5 675,708 - +0.1 675,708 - +0.1 675,708 - +0.5 675,708 - -0.9 675,708 - -0.7 675,708 - +0.4 675,953 + -0.3 675,953 + -1.8 675,953 + -0.8 675,953 + -1.0 675,953 + +0.6 675,953 + -1.5 675,954 - +0.2 675,954 - +0.1 675,954 - +0.5 675,954 - +0.4 675,954 - +0.6 675,954 - -1.6 675,992 + -0.7 675,992 + +0.5 675,992 + +0.3 675,992 + +1.1 675,993 - +0.4 675,993 - -0.2 675,993 - -0.3 675,993 - +0.3 675,993 - +0.1 675,993 - -1.5 675,993 - -0.5 676,350 - LRK54_RS03135 0.42 +0.2 676,350 - LRK54_RS03135 0.42 +0.6 676,350 - LRK54_RS03135 0.42 -0.1 676,417 - LRK54_RS03135 0.49 +1.1 676,460 + LRK54_RS03135 0.53 +0.0 676,499 + LRK54_RS03135 0.56 -1.2 676,499 + LRK54_RS03135 0.56 -1.2 676,499 + LRK54_RS03135 0.56 -0.3 676,499 + LRK54_RS03135 0.56 +0.5 676,499 + LRK54_RS03135 0.56 +0.2 676,499 + LRK54_RS03135 0.56 -0.9 676,499 + LRK54_RS03135 0.56 -0.2 676,500 - LRK54_RS03135 0.57 -0.7 676,500 - LRK54_RS03135 0.57 -0.0 676,500 - LRK54_RS03135 0.57 +0.2 676,500 - LRK54_RS03135 0.57 +1.1 676,500 - LRK54_RS03135 0.57 +0.1 676,500 - LRK54_RS03135 0.57 +0.4 676,500 - LRK54_RS03135 0.57 +0.5 676,500 - LRK54_RS03135 0.57 +0.5 676,500 - LRK54_RS03135 0.57 -1.0 676,500 - LRK54_RS03135 0.57 -0.9 676,500 - LRK54_RS03135 0.57 -1.8 676,500 - LRK54_RS03135 0.57 +0.0 676,500 - LRK54_RS03135 0.57 -0.4 676,742 + LRK54_RS03135 0.80 -0.0 676,742 + LRK54_RS03135 0.80 +1.2 676,742 + LRK54_RS03135 0.80 -0.4 676,742 + LRK54_RS03135 0.80 -0.3 676,742 + LRK54_RS03135 0.80 +0.2 676,742 + LRK54_RS03135 0.80 +0.3 676,743 - LRK54_RS03135 0.80 -1.1 676,952 + -1.2 676,953 - +0.8 676,953 - -0.2 676,953 - -0.2 676,953 - -0.5 676,953 - -0.0 676,953 - -0.8 676,953 - -0.5 676,953 - -0.9 676,953 - -0.8 676,953 - +1.2 676,968 + +0.1 676,968 + +0.1 676,968 + +0.5 676,968 + -0.6 676,968 + -1.9 676,968 + +0.5 676,968 + -0.0 676,968 + +0.7 676,968 + +0.9 676,968 + -0.8 676,968 + +0.6 676,968 + +1.8 676,968 + -1.3 676,968 + -0.1 676,969 - +0.9 676,969 - -0.0 676,969 - -1.3 676,969 - -2.2 676,969 - -0.3 676,969 - -0.3 676,969 - +0.3 676,969 - -0.8 676,969 - -0.1 676,969 - +0.1 676,969 - -0.4 676,969 - -1.1 676,969 - -0.6 676,980 + -0.5 676,980 + +1.4 676,981 - +0.3 676,981 - +0.1 676,981 - +0.2 677,014 + +0.3 677,014 + -0.1 677,014 + -0.6 677,014 + -0.2 677,014 + -0.1 677,014 + -1.1 677,014 + +0.4 677,014 + -1.1 677,014 + -0.1 677,014 + -0.0 677,015 - +0.1 677,015 - +0.4 677,015 - +1.3 677,015 - -0.7 677,017 - +0.5 677,017 - -0.4 677,017 - +1.4 677,017 - -0.2 677,017 - -0.3 677,044 + -0.5 677,095 + LRK54_RS03140 0.14 +0.4 677,095 + LRK54_RS03140 0.14 +0.0 677,095 + LRK54_RS03140 0.14 -0.4 677,095 + LRK54_RS03140 0.14 +0.1 677,095 + LRK54_RS03140 0.14 +0.8 677,095 + LRK54_RS03140 0.14 -0.9 677,095 + LRK54_RS03140 0.14 +0.9 677,095 + LRK54_RS03140 0.14 +0.3 677,095 + LRK54_RS03140 0.14 -0.6 677,095 + LRK54_RS03140 0.14 -0.3 677,095 + LRK54_RS03140 0.14 -0.1 677,095 + LRK54_RS03140 0.14 -0.4 677,095 + LRK54_RS03140 0.14 -1.1 677,095 + LRK54_RS03140 0.14 -0.1 677,095 + LRK54_RS03140 0.14 +0.3 677,095 + LRK54_RS03140 0.14 -1.6 677,095 + LRK54_RS03140 0.14 -0.3 677,095 + LRK54_RS03140 0.14 +0.5 677,095 + LRK54_RS03140 0.14 -0.3 677,095 + LRK54_RS03140 0.14 -0.4 677,096 - LRK54_RS03140 0.14 -0.4 677,096 - LRK54_RS03140 0.14 -0.4 677,096 - LRK54_RS03140 0.14 +0.1 677,096 - LRK54_RS03140 0.14 -0.4 677,096 - LRK54_RS03140 0.14 +0.1 677,096 - LRK54_RS03140 0.14 +2.2 677,096 - LRK54_RS03140 0.14 +0.2 677,096 - LRK54_RS03140 0.14 -1.2 677,096 - LRK54_RS03140 0.14 -0.9 677,096 - LRK54_RS03140 0.14 -1.5 677,096 - LRK54_RS03140 0.14 -0.2 677,096 - LRK54_RS03140 0.14 +0.4 677,096 - LRK54_RS03140 0.14 +0.4 677,096 - LRK54_RS03140 0.14 +0.1 677,096 - LRK54_RS03140 0.14 +0.7 677,096 - LRK54_RS03140 0.14 +1.2 677,314 + LRK54_RS03140 0.50 +0.3 677,314 + LRK54_RS03140 0.50 -0.0 677,315 - LRK54_RS03140 0.50 -0.3 677,315 - LRK54_RS03140 0.50 -0.3
Or see this region's nucleotide sequence