Experiment: R2A with Nickel (II) chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS09550 and CA265_RS09555 are separated by 58 nucleotides CA265_RS09555 and CA265_RS09560 are separated by 3 nucleotides CA265_RS09560 and CA265_RS09565 are separated by 76 nucleotides
CA265_RS09550: CA265_RS09550 - hypothetical protein, at 2,363,261 to 2,365,219
_RS09550
CA265_RS09555: CA265_RS09555 - phage envelope protein, at 2,365,278 to 2,365,673
_RS09555
CA265_RS09560: CA265_RS09560 - galactose-1-epimerase, at 2,365,677 to 2,366,726
_RS09560
CA265_RS09565: CA265_RS09565 - galactokinase, at 2,366,803 to 2,367,969
_RS09565
Position (kb)
2365
2366
2367 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 2364.717 kb on + strand, within CA265_RS09550 at 2364.812 kb on - strand, within CA265_RS09550 at 2364.835 kb on - strand, within CA265_RS09550 at 2364.835 kb on - strand, within CA265_RS09550 at 2364.835 kb on - strand, within CA265_RS09550 at 2364.847 kb on + strand, within CA265_RS09550 at 2364.848 kb on - strand, within CA265_RS09550 at 2364.855 kb on + strand, within CA265_RS09550 at 2364.870 kb on + strand, within CA265_RS09550 at 2364.873 kb on + strand, within CA265_RS09550 at 2364.873 kb on + strand, within CA265_RS09550 at 2364.889 kb on - strand, within CA265_RS09550 at 2364.962 kb on + strand, within CA265_RS09550 at 2364.962 kb on + strand, within CA265_RS09550 at 2364.976 kb on - strand, within CA265_RS09550 at 2364.980 kb on - strand, within CA265_RS09550 at 2364.980 kb on - strand, within CA265_RS09550 at 2365.078 kb on + strand at 2365.079 kb on - strand at 2365.196 kb on - strand at 2365.207 kb on + strand at 2365.217 kb on + strand at 2365.261 kb on + strand at 2365.279 kb on + strand at 2365.394 kb on + strand, within CA265_RS09555 at 2365.521 kb on - strand, within CA265_RS09555 at 2365.557 kb on + strand, within CA265_RS09555 at 2365.557 kb on + strand, within CA265_RS09555 at 2365.562 kb on - strand, within CA265_RS09555 at 2365.562 kb on - strand, within CA265_RS09555 at 2365.572 kb on - strand, within CA265_RS09555 at 2365.679 kb on - strand at 2365.697 kb on + strand at 2365.760 kb on + strand at 2365.761 kb on - strand at 2365.807 kb on + strand, within CA265_RS09560 at 2365.808 kb on - strand, within CA265_RS09560 at 2365.817 kb on - strand, within CA265_RS09560 at 2365.890 kb on - strand, within CA265_RS09560 at 2365.890 kb on - strand, within CA265_RS09560 at 2365.891 kb on + strand, within CA265_RS09560 at 2365.893 kb on + strand at 2365.984 kb on + strand, within CA265_RS09560 at 2366.031 kb on - strand, within CA265_RS09560 at 2366.084 kb on + strand, within CA265_RS09560 at 2366.136 kb on - strand, within CA265_RS09560 at 2366.168 kb on - strand, within CA265_RS09560 at 2366.218 kb on + strand, within CA265_RS09560 at 2366.223 kb on + strand, within CA265_RS09560 at 2366.224 kb on - strand, within CA265_RS09560 at 2366.224 kb on - strand, within CA265_RS09560 at 2366.240 kb on - strand, within CA265_RS09560 at 2366.364 kb on + strand, within CA265_RS09560 at 2366.383 kb on - strand, within CA265_RS09560 at 2366.385 kb on + strand, within CA265_RS09560 at 2366.388 kb on - strand, within CA265_RS09560 at 2366.397 kb on + strand, within CA265_RS09560 at 2366.397 kb on + strand, within CA265_RS09560 at 2366.397 kb on + strand, within CA265_RS09560 at 2366.397 kb on + strand, within CA265_RS09560 at 2366.402 kb on + strand, within CA265_RS09560 at 2366.403 kb on - strand, within CA265_RS09560 at 2366.490 kb on + strand, within CA265_RS09560 at 2366.490 kb on + strand, within CA265_RS09560 at 2366.491 kb on - strand, within CA265_RS09560 at 2366.491 kb on - strand, within CA265_RS09560 at 2366.503 kb on + strand, within CA265_RS09560 at 2366.509 kb on + strand, within CA265_RS09560 at 2366.624 kb on - strand at 2366.624 kb on - strand at 2366.641 kb on + strand at 2366.642 kb on - strand at 2366.791 kb on + strand at 2366.901 kb on + strand at 2366.902 kb on - strand at 2366.908 kb on + strand at 2366.909 kb on - strand at 2366.910 kb on + strand at 2366.941 kb on - strand, within CA265_RS09565 at 2366.961 kb on + strand, within CA265_RS09565 at 2366.962 kb on - strand, within CA265_RS09565 at 2366.962 kb on - strand, within CA265_RS09565 at 2366.984 kb on + strand, within CA265_RS09565 at 2366.984 kb on + strand, within CA265_RS09565 at 2366.985 kb on - strand, within CA265_RS09565 at 2367.026 kb on + strand, within CA265_RS09565 at 2367.057 kb on + strand, within CA265_RS09565 at 2367.063 kb on - strand, within CA265_RS09565 at 2367.063 kb on - strand, within CA265_RS09565 at 2367.195 kb on - strand, within CA265_RS09565 at 2367.216 kb on + strand, within CA265_RS09565 at 2367.217 kb on - strand, within CA265_RS09565 at 2367.220 kb on + strand, within CA265_RS09565 at 2367.221 kb on - strand, within CA265_RS09565 at 2367.225 kb on - strand, within CA265_RS09565 at 2367.225 kb on - strand, within CA265_RS09565 at 2367.230 kb on + strand, within CA265_RS09565 at 2367.356 kb on + strand, within CA265_RS09565 at 2367.372 kb on - strand, within CA265_RS09565 at 2367.526 kb on + strand, within CA265_RS09565 at 2367.593 kb on + strand, within CA265_RS09565 at 2367.615 kb on + strand, within CA265_RS09565 at 2367.616 kb on - strand, within CA265_RS09565 at 2367.645 kb on + strand, within CA265_RS09565 at 2367.646 kb on - strand, within CA265_RS09565 at 2367.691 kb on + strand, within CA265_RS09565 at 2367.692 kb on - strand, within CA265_RS09565 at 2367.710 kb on + strand, within CA265_RS09565 at 2367.719 kb on + strand, within CA265_RS09565 at 2367.719 kb on + strand, within CA265_RS09565 at 2367.719 kb on + strand, within CA265_RS09565 at 2367.720 kb on - strand, within CA265_RS09565 at 2367.721 kb on + strand, within CA265_RS09565
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.3 mM remove 2,364,717 + CA265_RS09550 0.74 +0.2 2,364,812 - CA265_RS09550 0.79 +0.7 2,364,835 - CA265_RS09550 0.80 +0.1 2,364,835 - CA265_RS09550 0.80 -0.5 2,364,835 - CA265_RS09550 0.80 -0.1 2,364,847 + CA265_RS09550 0.81 -0.7 2,364,848 - CA265_RS09550 0.81 +0.3 2,364,855 + CA265_RS09550 0.81 +0.9 2,364,870 + CA265_RS09550 0.82 +0.4 2,364,873 + CA265_RS09550 0.82 +0.0 2,364,873 + CA265_RS09550 0.82 -0.4 2,364,889 - CA265_RS09550 0.83 +0.1 2,364,962 + CA265_RS09550 0.87 -1.4 2,364,962 + CA265_RS09550 0.87 -2.1 2,364,976 - CA265_RS09550 0.88 -0.1 2,364,980 - CA265_RS09550 0.88 +0.4 2,364,980 - CA265_RS09550 0.88 -0.6 2,365,078 + -0.9 2,365,079 - -0.3 2,365,196 - +0.7 2,365,207 + +0.2 2,365,217 + -0.6 2,365,261 + +0.9 2,365,279 + +0.8 2,365,394 + CA265_RS09555 0.29 +0.4 2,365,521 - CA265_RS09555 0.61 +0.2 2,365,557 + CA265_RS09555 0.70 +0.3 2,365,557 + CA265_RS09555 0.70 +0.5 2,365,562 - CA265_RS09555 0.72 -0.7 2,365,562 - CA265_RS09555 0.72 -0.7 2,365,572 - CA265_RS09555 0.74 -0.5 2,365,679 - -1.3 2,365,697 + -2.2 2,365,760 + -4.5 2,365,761 - -2.2 2,365,807 + CA265_RS09560 0.12 -4.9 2,365,808 - CA265_RS09560 0.12 -3.9 2,365,817 - CA265_RS09560 0.13 -2.3 2,365,890 - CA265_RS09560 0.20 -0.8 2,365,890 - CA265_RS09560 0.20 -3.0 2,365,891 + CA265_RS09560 0.20 -2.9 2,365,893 + -0.8 2,365,984 + CA265_RS09560 0.29 -4.2 2,366,031 - CA265_RS09560 0.34 -3.2 2,366,084 + CA265_RS09560 0.39 -4.8 2,366,136 - CA265_RS09560 0.44 -3.4 2,366,168 - CA265_RS09560 0.47 -2.4 2,366,218 + CA265_RS09560 0.52 -2.5 2,366,223 + CA265_RS09560 0.52 -3.1 2,366,224 - CA265_RS09560 0.52 -4.3 2,366,224 - CA265_RS09560 0.52 -2.1 2,366,240 - CA265_RS09560 0.54 -4.5 2,366,364 + CA265_RS09560 0.65 -5.2 2,366,383 - CA265_RS09560 0.67 -2.4 2,366,385 + CA265_RS09560 0.67 -2.5 2,366,388 - CA265_RS09560 0.68 -1.6 2,366,397 + CA265_RS09560 0.69 -2.5 2,366,397 + CA265_RS09560 0.69 -2.4 2,366,397 + CA265_RS09560 0.69 -1.7 2,366,397 + CA265_RS09560 0.69 -1.5 2,366,402 + CA265_RS09560 0.69 -2.9 2,366,403 - CA265_RS09560 0.69 -3.3 2,366,490 + CA265_RS09560 0.77 -0.9 2,366,490 + CA265_RS09560 0.77 -3.4 2,366,491 - CA265_RS09560 0.78 -1.7 2,366,491 - CA265_RS09560 0.78 -3.3 2,366,503 + CA265_RS09560 0.79 -2.7 2,366,509 + CA265_RS09560 0.79 -2.3 2,366,624 - -2.9 2,366,624 - -1.4 2,366,641 + -0.4 2,366,642 - -0.4 2,366,791 + +0.2 2,366,901 + +0.3 2,366,902 - -0.0 2,366,908 + +0.2 2,366,909 - -0.2 2,366,910 + +2.2 2,366,941 - CA265_RS09565 0.12 -0.8 2,366,961 + CA265_RS09565 0.14 +0.8 2,366,962 - CA265_RS09565 0.14 +0.2 2,366,962 - CA265_RS09565 0.14 -1.2 2,366,984 + CA265_RS09565 0.16 -0.2 2,366,984 + CA265_RS09565 0.16 +0.5 2,366,985 - CA265_RS09565 0.16 +0.0 2,367,026 + CA265_RS09565 0.19 -0.2 2,367,057 + CA265_RS09565 0.22 +0.1 2,367,063 - CA265_RS09565 0.22 -0.1 2,367,063 - CA265_RS09565 0.22 -0.5 2,367,195 - CA265_RS09565 0.34 +0.4 2,367,216 + CA265_RS09565 0.35 +0.3 2,367,217 - CA265_RS09565 0.35 -0.3 2,367,220 + CA265_RS09565 0.36 -0.3 2,367,221 - CA265_RS09565 0.36 +0.2 2,367,225 - CA265_RS09565 0.36 -0.0 2,367,225 - CA265_RS09565 0.36 +0.0 2,367,230 + CA265_RS09565 0.37 +0.2 2,367,356 + CA265_RS09565 0.47 +0.1 2,367,372 - CA265_RS09565 0.49 +1.2 2,367,526 + CA265_RS09565 0.62 +0.0 2,367,593 + CA265_RS09565 0.68 -1.1 2,367,615 + CA265_RS09565 0.70 +0.7 2,367,616 - CA265_RS09565 0.70 -0.3 2,367,645 + CA265_RS09565 0.72 -0.4 2,367,646 - CA265_RS09565 0.72 -1.3 2,367,691 + CA265_RS09565 0.76 -0.1 2,367,692 - CA265_RS09565 0.76 +0.3 2,367,710 + CA265_RS09565 0.78 -1.1 2,367,719 + CA265_RS09565 0.78 +0.3 2,367,719 + CA265_RS09565 0.78 -0.9 2,367,719 + CA265_RS09565 0.78 -0.4 2,367,720 - CA265_RS09565 0.79 +0.0 2,367,721 + CA265_RS09565 0.79 -0.6
Or see this region's nucleotide sequence