Experiment: R2A with Nickel (II) chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS03160 and CA265_RS03165 are separated by 115 nucleotides CA265_RS03165 and CA265_RS03170 are separated by 50 nucleotides CA265_RS03170 and CA265_RS03175 are separated by 113 nucleotides
CA265_RS03160: CA265_RS03160 - hydrolase, at 780,789 to 781,433
_RS03160
CA265_RS03165: CA265_RS03165 - hypothetical protein, at 781,549 to 781,923
_RS03165
CA265_RS03170: CA265_RS03170 - short-chain dehydrogenase, at 781,974 to 782,675
_RS03170
CA265_RS03175: CA265_RS03175 - phenylalanine 4-monooxygenase, at 782,789 to 784,570
_RS03175
Position (kb)
781
782
783 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 781.018 kb on + strand, within CA265_RS03160 at 781.072 kb on + strand, within CA265_RS03160 at 781.072 kb on + strand, within CA265_RS03160 at 781.140 kb on + strand, within CA265_RS03160 at 781.141 kb on - strand, within CA265_RS03160 at 781.145 kb on + strand, within CA265_RS03160 at 781.215 kb on - strand, within CA265_RS03160 at 781.417 kb on - strand at 781.526 kb on - strand at 781.646 kb on - strand, within CA265_RS03165 at 781.677 kb on + strand, within CA265_RS03165 at 781.678 kb on - strand, within CA265_RS03165 at 781.679 kb on + strand, within CA265_RS03165 at 781.680 kb on - strand, within CA265_RS03165 at 781.680 kb on - strand, within CA265_RS03165 at 781.691 kb on - strand, within CA265_RS03165 at 781.693 kb on - strand, within CA265_RS03165 at 781.790 kb on + strand, within CA265_RS03165 at 781.813 kb on - strand, within CA265_RS03165 at 781.820 kb on + strand, within CA265_RS03165 at 781.821 kb on - strand, within CA265_RS03165 at 781.821 kb on - strand, within CA265_RS03165 at 781.821 kb on - strand, within CA265_RS03165 at 781.821 kb on - strand, within CA265_RS03165 at 781.821 kb on - strand, within CA265_RS03165 at 781.821 kb on - strand, within CA265_RS03165 at 781.852 kb on - strand, within CA265_RS03165 at 781.862 kb on + strand, within CA265_RS03165 at 781.862 kb on + strand, within CA265_RS03165 at 781.863 kb on - strand, within CA265_RS03165 at 781.967 kb on - strand at 781.972 kb on + strand at 782.051 kb on - strand, within CA265_RS03170 at 782.195 kb on + strand, within CA265_RS03170 at 782.195 kb on + strand, within CA265_RS03170 at 782.196 kb on - strand, within CA265_RS03170 at 782.199 kb on + strand, within CA265_RS03170 at 782.267 kb on + strand, within CA265_RS03170 at 782.305 kb on - strand, within CA265_RS03170 at 782.402 kb on + strand, within CA265_RS03170 at 782.403 kb on - strand, within CA265_RS03170 at 782.424 kb on - strand, within CA265_RS03170 at 782.490 kb on + strand, within CA265_RS03170 at 782.511 kb on + strand, within CA265_RS03170 at 782.532 kb on - strand, within CA265_RS03170 at 782.558 kb on + strand, within CA265_RS03170 at 782.665 kb on - strand at 782.743 kb on - strand at 782.922 kb on + strand at 782.932 kb on + strand at 782.993 kb on - strand, within CA265_RS03175 at 783.065 kb on + strand, within CA265_RS03175 at 783.066 kb on - strand, within CA265_RS03175 at 783.128 kb on + strand, within CA265_RS03175 at 783.133 kb on + strand, within CA265_RS03175 at 783.259 kb on + strand, within CA265_RS03175 at 783.304 kb on + strand, within CA265_RS03175 at 783.304 kb on + strand, within CA265_RS03175 at 783.305 kb on - strand, within CA265_RS03175 at 783.305 kb on - strand, within CA265_RS03175 at 783.305 kb on - strand, within CA265_RS03175 at 783.307 kb on + strand, within CA265_RS03175 at 783.320 kb on - strand, within CA265_RS03175 at 783.327 kb on - strand, within CA265_RS03175 at 783.347 kb on + strand, within CA265_RS03175 at 783.423 kb on - strand, within CA265_RS03175 at 783.434 kb on + strand, within CA265_RS03175 at 783.491 kb on + strand, within CA265_RS03175 at 783.539 kb on - strand, within CA265_RS03175 at 783.559 kb on + strand, within CA265_RS03175 at 783.650 kb on - strand, within CA265_RS03175
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.3 mM remove 781,018 + CA265_RS03160 0.36 +0.3 781,072 + CA265_RS03160 0.44 +1.0 781,072 + CA265_RS03160 0.44 -0.0 781,140 + CA265_RS03160 0.54 +1.2 781,141 - CA265_RS03160 0.55 -0.5 781,145 + CA265_RS03160 0.55 +0.3 781,215 - CA265_RS03160 0.66 +0.2 781,417 - -0.2 781,526 - +1.1 781,646 - CA265_RS03165 0.26 -0.4 781,677 + CA265_RS03165 0.34 -0.3 781,678 - CA265_RS03165 0.34 -0.4 781,679 + CA265_RS03165 0.35 -0.3 781,680 - CA265_RS03165 0.35 -0.5 781,680 - CA265_RS03165 0.35 -0.1 781,691 - CA265_RS03165 0.38 -1.1 781,693 - CA265_RS03165 0.38 -0.3 781,790 + CA265_RS03165 0.64 -0.3 781,813 - CA265_RS03165 0.70 +0.2 781,820 + CA265_RS03165 0.72 -0.3 781,821 - CA265_RS03165 0.73 +0.5 781,821 - CA265_RS03165 0.73 -0.2 781,821 - CA265_RS03165 0.73 -1.2 781,821 - CA265_RS03165 0.73 -0.3 781,821 - CA265_RS03165 0.73 -0.6 781,821 - CA265_RS03165 0.73 -1.4 781,852 - CA265_RS03165 0.81 -1.4 781,862 + CA265_RS03165 0.83 +0.4 781,862 + CA265_RS03165 0.83 +0.9 781,863 - CA265_RS03165 0.84 -0.3 781,967 - +0.8 781,972 + +0.2 782,051 - CA265_RS03170 0.11 -1.8 782,195 + CA265_RS03170 0.31 -1.9 782,195 + CA265_RS03170 0.31 -1.3 782,196 - CA265_RS03170 0.32 -1.7 782,199 + CA265_RS03170 0.32 -0.6 782,267 + CA265_RS03170 0.42 -1.9 782,305 - CA265_RS03170 0.47 -2.7 782,402 + CA265_RS03170 0.61 -1.3 782,403 - CA265_RS03170 0.61 -0.2 782,424 - CA265_RS03170 0.64 -1.4 782,490 + CA265_RS03170 0.74 -2.0 782,511 + CA265_RS03170 0.76 -0.8 782,532 - CA265_RS03170 0.79 -3.0 782,558 + CA265_RS03170 0.83 +0.1 782,665 - -3.4 782,743 - -0.6 782,922 + +0.2 782,932 + +0.2 782,993 - CA265_RS03175 0.11 -0.5 783,065 + CA265_RS03175 0.15 -0.8 783,066 - CA265_RS03175 0.16 -0.3 783,128 + CA265_RS03175 0.19 +1.0 783,133 + CA265_RS03175 0.19 +0.4 783,259 + CA265_RS03175 0.26 -0.8 783,304 + CA265_RS03175 0.29 +0.3 783,304 + CA265_RS03175 0.29 +0.3 783,305 - CA265_RS03175 0.29 +0.5 783,305 - CA265_RS03175 0.29 -0.1 783,305 - CA265_RS03175 0.29 +0.4 783,307 + CA265_RS03175 0.29 +0.9 783,320 - CA265_RS03175 0.30 -0.5 783,327 - CA265_RS03175 0.30 +0.2 783,347 + CA265_RS03175 0.31 +0.3 783,423 - CA265_RS03175 0.36 -0.1 783,434 + CA265_RS03175 0.36 +0.7 783,491 + CA265_RS03175 0.39 +0.1 783,539 - CA265_RS03175 0.42 -0.0 783,559 + CA265_RS03175 0.43 +0.3 783,650 - CA265_RS03175 0.48 +0.8
Or see this region's nucleotide sequence