Experiment: R2A with Nickel (II) chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02145 and CA265_RS02150 are separated by 757 nucleotides CA265_RS02150 and CA265_RS02155 are separated by 86 nucleotides CA265_RS02155 and CA265_RS02160 are separated by 22 nucleotides
CA265_RS02145: CA265_RS02145 - 1,4-alpha-glucan branching enzyme, at 541,505 to 543,469
_RS02145
CA265_RS02150: CA265_RS02150 - hypothetical protein, at 544,227 to 546,386
_RS02150
CA265_RS02155: CA265_RS02155 - hypothetical protein, at 546,473 to 547,144
_RS02155
CA265_RS02160: CA265_RS02160 - hypothetical protein, at 547,167 to 548,108
_RS02160
Position (kb) 544
545
546
547 Strain fitness (log2 ratio) -2
-1
0
1
2 at 543.235 kb on + strand, within CA265_RS02145 at 543.337 kb on + strand at 543.342 kb on + strand at 543.343 kb on - strand at 543.343 kb on - strand at 543.377 kb on - strand at 543.377 kb on - strand at 543.377 kb on - strand at 543.378 kb on + strand at 543.378 kb on + strand at 543.379 kb on - strand at 543.379 kb on - strand at 543.379 kb on - strand at 543.399 kb on - strand at 543.400 kb on + strand at 543.401 kb on - strand at 543.421 kb on - strand at 543.485 kb on - strand at 543.486 kb on + strand at 543.486 kb on + strand at 543.487 kb on - strand at 543.511 kb on + strand at 543.593 kb on - strand at 543.645 kb on + strand at 543.660 kb on + strand at 543.748 kb on + strand at 543.757 kb on + strand at 543.772 kb on + strand at 543.789 kb on + strand at 543.800 kb on + strand at 543.820 kb on + strand at 543.951 kb on - strand at 543.959 kb on + strand at 543.959 kb on + strand at 543.963 kb on + strand at 543.964 kb on - strand at 544.132 kb on - strand at 544.132 kb on - strand at 544.236 kb on + strand at 544.236 kb on + strand at 544.293 kb on + strand at 544.294 kb on - strand at 544.306 kb on + strand at 544.374 kb on - strand at 544.483 kb on + strand, within CA265_RS02150 at 544.484 kb on - strand, within CA265_RS02150 at 544.493 kb on + strand, within CA265_RS02150 at 544.532 kb on - strand, within CA265_RS02150 at 544.568 kb on + strand at 544.568 kb on + strand, within CA265_RS02150 at 544.655 kb on + strand, within CA265_RS02150 at 544.656 kb on - strand, within CA265_RS02150 at 544.744 kb on + strand, within CA265_RS02150 at 544.769 kb on + strand, within CA265_RS02150 at 544.778 kb on - strand, within CA265_RS02150 at 544.801 kb on - strand, within CA265_RS02150 at 544.814 kb on - strand, within CA265_RS02150 at 544.852 kb on + strand, within CA265_RS02150 at 544.852 kb on + strand, within CA265_RS02150 at 544.905 kb on - strand, within CA265_RS02150 at 544.905 kb on - strand, within CA265_RS02150 at 544.905 kb on - strand, within CA265_RS02150 at 544.951 kb on - strand, within CA265_RS02150 at 544.962 kb on + strand, within CA265_RS02150 at 544.993 kb on - strand, within CA265_RS02150 at 544.997 kb on - strand, within CA265_RS02150 at 544.998 kb on - strand, within CA265_RS02150 at 545.039 kb on - strand, within CA265_RS02150 at 545.104 kb on + strand, within CA265_RS02150 at 545.104 kb on + strand, within CA265_RS02150 at 545.105 kb on - strand, within CA265_RS02150 at 545.105 kb on - strand, within CA265_RS02150 at 545.156 kb on + strand, within CA265_RS02150 at 545.199 kb on + strand, within CA265_RS02150 at 545.230 kb on - strand, within CA265_RS02150 at 545.230 kb on - strand, within CA265_RS02150 at 545.247 kb on - strand, within CA265_RS02150 at 545.340 kb on - strand, within CA265_RS02150 at 545.415 kb on + strand, within CA265_RS02150 at 545.415 kb on + strand, within CA265_RS02150 at 545.425 kb on - strand, within CA265_RS02150 at 545.449 kb on + strand, within CA265_RS02150 at 545.450 kb on - strand, within CA265_RS02150 at 545.469 kb on + strand, within CA265_RS02150 at 545.472 kb on - strand, within CA265_RS02150 at 545.484 kb on - strand, within CA265_RS02150 at 545.503 kb on - strand, within CA265_RS02150 at 545.526 kb on - strand, within CA265_RS02150 at 545.532 kb on + strand, within CA265_RS02150 at 545.633 kb on + strand, within CA265_RS02150 at 545.634 kb on - strand, within CA265_RS02150 at 545.720 kb on + strand, within CA265_RS02150 at 545.727 kb on + strand, within CA265_RS02150 at 545.728 kb on - strand, within CA265_RS02150 at 545.728 kb on - strand, within CA265_RS02150 at 545.729 kb on + strand, within CA265_RS02150 at 545.730 kb on - strand, within CA265_RS02150 at 545.730 kb on - strand, within CA265_RS02150 at 545.730 kb on - strand, within CA265_RS02150 at 545.795 kb on + strand, within CA265_RS02150 at 545.796 kb on - strand, within CA265_RS02150 at 545.882 kb on + strand, within CA265_RS02150 at 545.959 kb on + strand, within CA265_RS02150 at 545.967 kb on - strand, within CA265_RS02150 at 545.978 kb on + strand, within CA265_RS02150 at 546.013 kb on - strand, within CA265_RS02150 at 546.032 kb on + strand, within CA265_RS02150 at 546.059 kb on + strand, within CA265_RS02150 at 546.102 kb on - strand, within CA265_RS02150 at 546.125 kb on + strand, within CA265_RS02150 at 546.126 kb on - strand, within CA265_RS02150 at 546.126 kb on - strand, within CA265_RS02150 at 546.129 kb on - strand, within CA265_RS02150 at 546.149 kb on + strand, within CA265_RS02150 at 546.243 kb on - strand at 546.279 kb on + strand at 546.280 kb on - strand at 546.373 kb on + strand at 546.378 kb on - strand at 546.378 kb on - strand at 546.385 kb on - strand at 546.418 kb on + strand at 546.453 kb on + strand at 546.465 kb on + strand at 546.465 kb on + strand at 546.482 kb on - strand at 546.517 kb on - strand at 546.519 kb on - strand at 546.567 kb on - strand, within CA265_RS02155 at 546.586 kb on + strand, within CA265_RS02155 at 546.631 kb on - strand, within CA265_RS02155 at 546.632 kb on + strand, within CA265_RS02155 at 546.633 kb on - strand, within CA265_RS02155 at 546.633 kb on - strand, within CA265_RS02155 at 546.692 kb on + strand, within CA265_RS02155 at 546.693 kb on - strand, within CA265_RS02155 at 546.748 kb on + strand, within CA265_RS02155 at 546.749 kb on - strand, within CA265_RS02155 at 546.779 kb on - strand, within CA265_RS02155 at 546.779 kb on - strand, within CA265_RS02155 at 546.781 kb on + strand, within CA265_RS02155 at 546.842 kb on - strand, within CA265_RS02155 at 546.870 kb on + strand, within CA265_RS02155 at 546.893 kb on + strand, within CA265_RS02155 at 546.893 kb on + strand, within CA265_RS02155 at 546.894 kb on - strand, within CA265_RS02155 at 546.912 kb on + strand, within CA265_RS02155 at 546.913 kb on - strand, within CA265_RS02155 at 546.928 kb on - strand, within CA265_RS02155 at 547.007 kb on + strand, within CA265_RS02155 at 547.102 kb on + strand at 547.117 kb on + strand at 547.126 kb on + strand at 547.126 kb on + strand at 547.127 kb on - strand at 547.142 kb on + strand at 547.149 kb on + strand at 547.193 kb on - strand at 547.256 kb on + strand at 547.347 kb on - strand, within CA265_RS02160 at 547.372 kb on - strand, within CA265_RS02160
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.3 mM remove 543,235 + CA265_RS02145 0.88 +1.2 543,337 + +0.4 543,342 + +0.2 543,343 - +0.5 543,343 - +0.2 543,377 - +1.6 543,377 - +1.8 543,377 - -0.2 543,378 + +1.7 543,378 + +0.9 543,379 - +1.0 543,379 - +1.0 543,379 - +0.4 543,399 - +0.8 543,400 + +1.3 543,401 - +0.9 543,421 - +0.5 543,485 - -0.4 543,486 + +0.0 543,486 + -1.4 543,487 - +0.9 543,511 + -0.8 543,593 - +0.0 543,645 + +1.3 543,660 + +0.7 543,748 + +0.0 543,757 + +0.6 543,772 + +0.7 543,789 + +0.1 543,800 + +0.2 543,820 + +1.3 543,951 - -0.2 543,959 + -0.2 543,959 + -0.8 543,963 + -0.3 543,964 - +0.0 544,132 - -0.2 544,132 - -0.2 544,236 + -0.0 544,236 + +1.0 544,293 + -0.8 544,294 - -0.7 544,306 + -0.6 544,374 - -0.5 544,483 + CA265_RS02150 0.12 -1.5 544,484 - CA265_RS02150 0.12 +1.6 544,493 + CA265_RS02150 0.12 -0.0 544,532 - CA265_RS02150 0.14 +0.6 544,568 + +0.0 544,568 + CA265_RS02150 0.16 -0.4 544,655 + CA265_RS02150 0.20 -1.9 544,656 - CA265_RS02150 0.20 +0.0 544,744 + CA265_RS02150 0.24 +1.0 544,769 + CA265_RS02150 0.25 -0.4 544,778 - CA265_RS02150 0.26 +1.6 544,801 - CA265_RS02150 0.27 -0.3 544,814 - CA265_RS02150 0.27 +0.4 544,852 + CA265_RS02150 0.29 -0.6 544,852 + CA265_RS02150 0.29 +0.8 544,905 - CA265_RS02150 0.31 +0.2 544,905 - CA265_RS02150 0.31 +0.4 544,905 - CA265_RS02150 0.31 +0.9 544,951 - CA265_RS02150 0.34 -0.7 544,962 + CA265_RS02150 0.34 -0.3 544,993 - CA265_RS02150 0.35 +0.2 544,997 - CA265_RS02150 0.36 -0.0 544,998 - CA265_RS02150 0.36 +0.6 545,039 - CA265_RS02150 0.38 -0.1 545,104 + CA265_RS02150 0.41 +0.0 545,104 + CA265_RS02150 0.41 +1.8 545,105 - CA265_RS02150 0.41 -0.4 545,105 - CA265_RS02150 0.41 -0.4 545,156 + CA265_RS02150 0.43 +0.1 545,199 + CA265_RS02150 0.45 -1.4 545,230 - CA265_RS02150 0.46 +0.8 545,230 - CA265_RS02150 0.46 +0.9 545,247 - CA265_RS02150 0.47 +0.2 545,340 - CA265_RS02150 0.52 -0.6 545,415 + CA265_RS02150 0.55 -0.0 545,415 + CA265_RS02150 0.55 -0.4 545,425 - CA265_RS02150 0.55 +0.1 545,449 + CA265_RS02150 0.57 -0.7 545,450 - CA265_RS02150 0.57 -0.1 545,469 + CA265_RS02150 0.57 -0.4 545,472 - CA265_RS02150 0.58 +0.4 545,484 - CA265_RS02150 0.58 -0.7 545,503 - CA265_RS02150 0.59 -0.7 545,526 - CA265_RS02150 0.60 +0.8 545,532 + CA265_RS02150 0.60 -0.3 545,633 + CA265_RS02150 0.65 +0.3 545,634 - CA265_RS02150 0.65 +0.1 545,720 + CA265_RS02150 0.69 -0.4 545,727 + CA265_RS02150 0.69 +1.4 545,728 - CA265_RS02150 0.69 -0.7 545,728 - CA265_RS02150 0.69 -0.1 545,729 + CA265_RS02150 0.70 +0.8 545,730 - CA265_RS02150 0.70 +1.3 545,730 - CA265_RS02150 0.70 +1.9 545,730 - CA265_RS02150 0.70 +0.5 545,795 + CA265_RS02150 0.73 +0.9 545,796 - CA265_RS02150 0.73 -0.2 545,882 + CA265_RS02150 0.77 -0.1 545,959 + CA265_RS02150 0.80 -0.2 545,967 - CA265_RS02150 0.81 -0.4 545,978 + CA265_RS02150 0.81 +0.0 546,013 - CA265_RS02150 0.83 -1.1 546,032 + CA265_RS02150 0.84 +0.4 546,059 + CA265_RS02150 0.85 +0.5 546,102 - CA265_RS02150 0.87 -0.0 546,125 + CA265_RS02150 0.88 -0.1 546,126 - CA265_RS02150 0.88 -0.3 546,126 - CA265_RS02150 0.88 +0.6 546,129 - CA265_RS02150 0.88 -0.3 546,149 + CA265_RS02150 0.89 -2.0 546,243 - -0.3 546,279 + -0.3 546,280 - -0.4 546,373 + -1.6 546,378 - -0.8 546,378 - -1.4 546,385 - +0.9 546,418 + -0.7 546,453 + +0.4 546,465 + -0.4 546,465 + -1.2 546,482 - +0.4 546,517 - -0.1 546,519 - -1.7 546,567 - CA265_RS02155 0.14 +0.1 546,586 + CA265_RS02155 0.17 +0.0 546,631 - CA265_RS02155 0.24 -2.6 546,632 + CA265_RS02155 0.24 -0.3 546,633 - CA265_RS02155 0.24 -0.9 546,633 - CA265_RS02155 0.24 -0.6 546,692 + CA265_RS02155 0.33 -0.9 546,693 - CA265_RS02155 0.33 -1.7 546,748 + CA265_RS02155 0.41 -0.2 546,749 - CA265_RS02155 0.41 -0.7 546,779 - CA265_RS02155 0.46 -0.6 546,779 - CA265_RS02155 0.46 -0.3 546,781 + CA265_RS02155 0.46 -0.9 546,842 - CA265_RS02155 0.55 -0.4 546,870 + CA265_RS02155 0.59 -0.5 546,893 + CA265_RS02155 0.62 -1.1 546,893 + CA265_RS02155 0.62 +0.2 546,894 - CA265_RS02155 0.63 -2.5 546,912 + CA265_RS02155 0.65 -0.7 546,913 - CA265_RS02155 0.65 +0.1 546,928 - CA265_RS02155 0.68 -0.6 547,007 + CA265_RS02155 0.79 +0.7 547,102 + -0.4 547,117 + +1.3 547,126 + -1.7 547,126 + -0.9 547,127 - +0.8 547,142 + +0.9 547,149 + -0.1 547,193 - -0.3 547,256 + +0.9 547,347 - CA265_RS02160 0.19 +0.2 547,372 - CA265_RS02160 0.22 -0.4
Or see this region's nucleotide sequence