Experiment: R2A with Nickel (II) chloride 0.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CA265_RS02135 and CA265_RS02140 are separated by 442 nucleotides CA265_RS02140 and CA265_RS02145 are separated by 59 nucleotides
CA265_RS02135: CA265_RS02135 - TonB-dependent receptor, at 535,649 to 538,540
_RS02135
CA265_RS02140: CA265_RS02140 - glycosyl hydrolase, at 538,983 to 541,445
_RS02140
CA265_RS02145: CA265_RS02145 - 1,4-alpha-glucan branching enzyme, at 541,505 to 543,469
_RS02145
Position (kb) 538
539
540
541
542 Strain fitness (log2 ratio) -2
-1
0
1 at 537.991 kb on - strand, within CA265_RS02135 at 538.017 kb on - strand, within CA265_RS02135 at 538.022 kb on + strand, within CA265_RS02135 at 538.022 kb on + strand, within CA265_RS02135 at 538.023 kb on - strand, within CA265_RS02135 at 538.025 kb on + strand, within CA265_RS02135 at 538.025 kb on + strand, within CA265_RS02135 at 538.026 kb on - strand, within CA265_RS02135 at 538.026 kb on - strand, within CA265_RS02135 at 538.035 kb on - strand, within CA265_RS02135 at 538.079 kb on - strand, within CA265_RS02135 at 538.092 kb on - strand, within CA265_RS02135 at 538.095 kb on + strand, within CA265_RS02135 at 538.132 kb on + strand, within CA265_RS02135 at 538.133 kb on - strand, within CA265_RS02135 at 538.152 kb on + strand, within CA265_RS02135 at 538.175 kb on - strand, within CA265_RS02135 at 538.198 kb on - strand, within CA265_RS02135 at 538.198 kb on - strand, within CA265_RS02135 at 538.202 kb on + strand, within CA265_RS02135 at 538.203 kb on - strand, within CA265_RS02135 at 538.229 kb on - strand, within CA265_RS02135 at 538.232 kb on + strand, within CA265_RS02135 at 538.232 kb on + strand, within CA265_RS02135 at 538.234 kb on + strand, within CA265_RS02135 at 538.235 kb on - strand, within CA265_RS02135 at 538.268 kb on - strand at 538.268 kb on - strand at 538.268 kb on - strand at 538.268 kb on - strand at 538.322 kb on + strand at 538.392 kb on + strand at 538.393 kb on - strand at 538.393 kb on - strand at 538.425 kb on + strand at 538.457 kb on - strand at 538.518 kb on + strand at 538.526 kb on - strand at 538.539 kb on - strand at 538.568 kb on + strand at 538.568 kb on + strand at 538.580 kb on - strand at 538.580 kb on - strand at 538.582 kb on - strand at 538.647 kb on - strand at 538.671 kb on - strand at 538.682 kb on - strand at 538.687 kb on + strand at 538.688 kb on - strand at 538.738 kb on + strand at 538.739 kb on - strand at 538.747 kb on + strand at 538.784 kb on + strand at 538.813 kb on + strand at 538.814 kb on - strand at 538.916 kb on + strand at 538.916 kb on + strand at 538.920 kb on - strand at 538.932 kb on + strand at 538.978 kb on + strand at 538.978 kb on + strand at 538.978 kb on + strand at 539.053 kb on + strand at 539.053 kb on + strand at 539.066 kb on - strand at 539.070 kb on - strand at 539.168 kb on - strand at 539.192 kb on + strand at 539.193 kb on - strand at 539.193 kb on - strand at 539.318 kb on + strand, within CA265_RS02140 at 539.318 kb on + strand, within CA265_RS02140 at 539.375 kb on + strand, within CA265_RS02140 at 539.389 kb on + strand, within CA265_RS02140 at 539.389 kb on + strand, within CA265_RS02140 at 539.393 kb on + strand, within CA265_RS02140 at 539.393 kb on + strand, within CA265_RS02140 at 539.414 kb on + strand, within CA265_RS02140 at 539.453 kb on + strand, within CA265_RS02140 at 539.457 kb on + strand, within CA265_RS02140 at 539.458 kb on - strand, within CA265_RS02140 at 539.501 kb on - strand, within CA265_RS02140 at 539.501 kb on - strand, within CA265_RS02140 at 539.507 kb on + strand, within CA265_RS02140 at 539.555 kb on + strand, within CA265_RS02140 at 539.613 kb on + strand, within CA265_RS02140 at 539.661 kb on - strand, within CA265_RS02140 at 539.666 kb on + strand, within CA265_RS02140 at 539.698 kb on + strand, within CA265_RS02140 at 539.763 kb on - strand, within CA265_RS02140 at 539.763 kb on - strand, within CA265_RS02140 at 539.884 kb on + strand, within CA265_RS02140 at 539.929 kb on - strand, within CA265_RS02140 at 539.943 kb on + strand, within CA265_RS02140 at 539.944 kb on - strand, within CA265_RS02140 at 539.944 kb on - strand, within CA265_RS02140 at 539.944 kb on - strand, within CA265_RS02140 at 540.033 kb on - strand, within CA265_RS02140 at 540.041 kb on - strand, within CA265_RS02140 at 540.068 kb on - strand, within CA265_RS02140 at 540.079 kb on - strand, within CA265_RS02140 at 540.089 kb on + strand, within CA265_RS02140 at 540.139 kb on - strand, within CA265_RS02140 at 540.141 kb on - strand, within CA265_RS02140 at 540.186 kb on - strand, within CA265_RS02140 at 540.216 kb on - strand, within CA265_RS02140 at 540.221 kb on - strand, within CA265_RS02140 at 540.334 kb on - strand, within CA265_RS02140 at 540.359 kb on + strand, within CA265_RS02140 at 540.359 kb on + strand, within CA265_RS02140 at 540.360 kb on - strand, within CA265_RS02140 at 540.360 kb on - strand, within CA265_RS02140 at 540.389 kb on + strand, within CA265_RS02140 at 540.389 kb on + strand, within CA265_RS02140 at 540.471 kb on + strand, within CA265_RS02140 at 540.497 kb on + strand, within CA265_RS02140 at 540.498 kb on - strand, within CA265_RS02140 at 540.517 kb on + strand, within CA265_RS02140 at 540.581 kb on + strand, within CA265_RS02140 at 540.638 kb on - strand, within CA265_RS02140 at 540.656 kb on + strand, within CA265_RS02140 at 540.680 kb on + strand, within CA265_RS02140 at 540.734 kb on + strand, within CA265_RS02140 at 540.753 kb on + strand, within CA265_RS02140 at 540.754 kb on - strand, within CA265_RS02140 at 540.817 kb on + strand, within CA265_RS02140 at 540.891 kb on - strand, within CA265_RS02140 at 540.891 kb on - strand, within CA265_RS02140 at 540.899 kb on - strand, within CA265_RS02140 at 541.005 kb on + strand, within CA265_RS02140 at 541.006 kb on - strand, within CA265_RS02140 at 541.008 kb on - strand, within CA265_RS02140 at 541.008 kb on - strand, within CA265_RS02140 at 541.032 kb on - strand, within CA265_RS02140 at 541.087 kb on - strand, within CA265_RS02140 at 541.118 kb on - strand, within CA265_RS02140 at 541.161 kb on + strand, within CA265_RS02140 at 541.161 kb on + strand, within CA265_RS02140 at 541.162 kb on - strand, within CA265_RS02140 at 541.276 kb on - strand at 541.276 kb on - strand at 541.276 kb on - strand at 541.284 kb on - strand at 541.287 kb on + strand at 541.287 kb on + strand at 541.344 kb on - strand at 541.349 kb on + strand at 541.394 kb on - strand at 541.470 kb on + strand at 541.470 kb on + strand at 541.471 kb on - strand at 541.588 kb on - strand at 541.603 kb on + strand at 541.662 kb on + strand at 541.666 kb on + strand at 541.725 kb on + strand, within CA265_RS02145 at 541.813 kb on + strand, within CA265_RS02145 at 541.841 kb on + strand, within CA265_RS02145 at 541.842 kb on - strand, within CA265_RS02145 at 541.848 kb on - strand, within CA265_RS02145 at 541.882 kb on - strand at 542.005 kb on - strand, within CA265_RS02145 at 542.037 kb on + strand, within CA265_RS02145 at 542.037 kb on + strand, within CA265_RS02145 at 542.038 kb on - strand, within CA265_RS02145 at 542.134 kb on + strand, within CA265_RS02145 at 542.143 kb on - strand, within CA265_RS02145 at 542.197 kb on + strand, within CA265_RS02145 at 542.204 kb on + strand, within CA265_RS02145 at 542.244 kb on + strand, within CA265_RS02145 at 542.245 kb on - strand, within CA265_RS02145 at 542.245 kb on - strand, within CA265_RS02145 at 542.245 kb on - strand, within CA265_RS02145 at 542.249 kb on + strand, within CA265_RS02145 at 542.250 kb on - strand, within CA265_RS02145 at 542.337 kb on - strand, within CA265_RS02145 at 542.410 kb on + strand, within CA265_RS02145 at 542.421 kb on - strand, within CA265_RS02145
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Nickel (II) chloride 0.3 mM remove 537,991 - CA265_RS02135 0.81 +0.1 538,017 - CA265_RS02135 0.82 +0.1 538,022 + CA265_RS02135 0.82 -1.1 538,022 + CA265_RS02135 0.82 +0.6 538,023 - CA265_RS02135 0.82 -0.3 538,025 + CA265_RS02135 0.82 -0.4 538,025 + CA265_RS02135 0.82 -0.3 538,026 - CA265_RS02135 0.82 -0.4 538,026 - CA265_RS02135 0.82 +0.3 538,035 - CA265_RS02135 0.83 +0.1 538,079 - CA265_RS02135 0.84 +0.4 538,092 - CA265_RS02135 0.84 +0.1 538,095 + CA265_RS02135 0.85 -0.0 538,132 + CA265_RS02135 0.86 +0.2 538,133 - CA265_RS02135 0.86 +0.9 538,152 + CA265_RS02135 0.87 +0.0 538,175 - CA265_RS02135 0.87 +1.0 538,198 - CA265_RS02135 0.88 +0.3 538,198 - CA265_RS02135 0.88 +0.0 538,202 + CA265_RS02135 0.88 -0.0 538,203 - CA265_RS02135 0.88 +0.2 538,229 - CA265_RS02135 0.89 +0.7 538,232 + CA265_RS02135 0.89 -0.3 538,232 + CA265_RS02135 0.89 -0.3 538,234 + CA265_RS02135 0.89 -0.4 538,235 - CA265_RS02135 0.89 -0.3 538,268 - +0.3 538,268 - -0.2 538,268 - +0.2 538,268 - -0.2 538,322 + +0.6 538,392 + -0.6 538,393 - +0.4 538,393 - -0.2 538,425 + -0.5 538,457 - +0.2 538,518 + -0.6 538,526 - +0.5 538,539 - +0.4 538,568 + +1.1 538,568 + -0.7 538,580 - -0.1 538,580 - -0.9 538,582 - -2.1 538,647 - -0.8 538,671 - -0.3 538,682 - +0.1 538,687 + +0.4 538,688 - -0.6 538,738 + -0.8 538,739 - +0.1 538,747 + -0.6 538,784 + +0.6 538,813 + +0.3 538,814 - +0.6 538,916 + +0.4 538,916 + -0.5 538,920 - -1.4 538,932 + -0.3 538,978 + -1.1 538,978 + +0.3 538,978 + +0.6 539,053 + -1.7 539,053 + +0.2 539,066 - -0.2 539,070 - +0.5 539,168 - +0.4 539,192 + +0.7 539,193 - +0.9 539,193 - +0.3 539,318 + CA265_RS02140 0.14 -1.0 539,318 + CA265_RS02140 0.14 +1.0 539,375 + CA265_RS02140 0.16 -1.3 539,389 + CA265_RS02140 0.16 -0.3 539,389 + CA265_RS02140 0.16 -1.0 539,393 + CA265_RS02140 0.17 +0.4 539,393 + CA265_RS02140 0.17 +0.1 539,414 + CA265_RS02140 0.17 +0.5 539,453 + CA265_RS02140 0.19 -0.5 539,457 + CA265_RS02140 0.19 -0.4 539,458 - CA265_RS02140 0.19 +0.6 539,501 - CA265_RS02140 0.21 +0.1 539,501 - CA265_RS02140 0.21 -0.3 539,507 + CA265_RS02140 0.21 +0.2 539,555 + CA265_RS02140 0.23 +0.0 539,613 + CA265_RS02140 0.26 -0.1 539,661 - CA265_RS02140 0.28 +0.8 539,666 + CA265_RS02140 0.28 -0.5 539,698 + CA265_RS02140 0.29 -1.1 539,763 - CA265_RS02140 0.32 +1.0 539,763 - CA265_RS02140 0.32 +0.0 539,884 + CA265_RS02140 0.37 -0.7 539,929 - CA265_RS02140 0.38 +0.3 539,943 + CA265_RS02140 0.39 -1.2 539,944 - CA265_RS02140 0.39 +0.7 539,944 - CA265_RS02140 0.39 +0.1 539,944 - CA265_RS02140 0.39 -0.2 540,033 - CA265_RS02140 0.43 +0.2 540,041 - CA265_RS02140 0.43 +0.4 540,068 - CA265_RS02140 0.44 +0.0 540,079 - CA265_RS02140 0.44 -0.2 540,089 + CA265_RS02140 0.45 -0.5 540,139 - CA265_RS02140 0.47 +0.5 540,141 - CA265_RS02140 0.47 +1.6 540,186 - CA265_RS02140 0.49 +1.0 540,216 - CA265_RS02140 0.50 +0.9 540,221 - CA265_RS02140 0.50 +0.4 540,334 - CA265_RS02140 0.55 +0.9 540,359 + CA265_RS02140 0.56 -1.3 540,359 + CA265_RS02140 0.56 -0.3 540,360 - CA265_RS02140 0.56 +0.8 540,360 - CA265_RS02140 0.56 +1.3 540,389 + CA265_RS02140 0.57 -0.8 540,389 + CA265_RS02140 0.57 +0.5 540,471 + CA265_RS02140 0.60 +0.5 540,497 + CA265_RS02140 0.61 +1.1 540,498 - CA265_RS02140 0.62 +0.9 540,517 + CA265_RS02140 0.62 -0.6 540,581 + CA265_RS02140 0.65 -0.4 540,638 - CA265_RS02140 0.67 +0.2 540,656 + CA265_RS02140 0.68 -0.9 540,680 + CA265_RS02140 0.69 +0.1 540,734 + CA265_RS02140 0.71 -0.5 540,753 + CA265_RS02140 0.72 -0.5 540,754 - CA265_RS02140 0.72 +0.2 540,817 + CA265_RS02140 0.74 +0.2 540,891 - CA265_RS02140 0.77 +0.6 540,891 - CA265_RS02140 0.77 +1.2 540,899 - CA265_RS02140 0.78 +0.3 541,005 + CA265_RS02140 0.82 -1.5 541,006 - CA265_RS02140 0.82 +0.3 541,008 - CA265_RS02140 0.82 +0.3 541,008 - CA265_RS02140 0.82 +0.3 541,032 - CA265_RS02140 0.83 +0.7 541,087 - CA265_RS02140 0.85 +0.3 541,118 - CA265_RS02140 0.87 +1.0 541,161 + CA265_RS02140 0.88 +0.3 541,161 + CA265_RS02140 0.88 +0.0 541,162 - CA265_RS02140 0.88 -0.4 541,276 - +0.9 541,276 - +0.3 541,276 - +0.9 541,284 - +0.6 541,287 + -0.7 541,287 + -0.7 541,344 - +0.4 541,349 + -0.8 541,394 - +0.9 541,470 + +0.9 541,470 + -0.9 541,471 - +0.8 541,588 - +0.3 541,603 + +1.1 541,662 + +0.6 541,666 + -0.2 541,725 + CA265_RS02145 0.11 +0.8 541,813 + CA265_RS02145 0.16 +0.0 541,841 + CA265_RS02145 0.17 +0.4 541,842 - CA265_RS02145 0.17 +0.7 541,848 - CA265_RS02145 0.17 +0.8 541,882 - +1.0 542,005 - CA265_RS02145 0.25 +0.4 542,037 + CA265_RS02145 0.27 +0.3 542,037 + CA265_RS02145 0.27 +1.5 542,038 - CA265_RS02145 0.27 +0.9 542,134 + CA265_RS02145 0.32 +0.5 542,143 - CA265_RS02145 0.32 -0.1 542,197 + CA265_RS02145 0.35 +1.2 542,204 + CA265_RS02145 0.36 +0.6 542,244 + CA265_RS02145 0.38 +0.9 542,245 - CA265_RS02145 0.38 +0.7 542,245 - CA265_RS02145 0.38 +0.5 542,245 - CA265_RS02145 0.38 +0.4 542,249 + CA265_RS02145 0.38 +0.7 542,250 - CA265_RS02145 0.38 +0.4 542,337 - CA265_RS02145 0.42 +0.6 542,410 + CA265_RS02145 0.46 +0.7 542,421 - CA265_RS02145 0.47 +0.6
Or see this region's nucleotide sequence