Strain Fitness in Cupriavidus basilensis FW507-4G11 around RR42_RS35740

Experiment: D-Lactate (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntRR42_RS35730 and RR42_RS35735 are separated by 165 nucleotidesRR42_RS35735 and RR42_RS35740 are separated by 54 nucleotidesRR42_RS35740 and RR42_RS35745 are separated by 231 nucleotidesRR42_RS35745 and RR42_RS35750 overlap by 4 nucleotides RR42_RS35730: RR42_RS35730 - flagellar basal body P-ring biosynthesis protein FlgA, at 3,465,276 to 3,465,995 _RS35730 RR42_RS35735: RR42_RS35735 - flagellar biosynthesis protein FlgM, at 3,466,161 to 3,466,466 _RS35735 RR42_RS35740: RR42_RS35740 - flagellar biosynthesis protein FlgN, at 3,466,521 to 3,466,979 _RS35740 RR42_RS35745: RR42_RS35745 - flagellar biosynthesis protein FlhE, at 3,467,211 to 3,467,642 _RS35745 RR42_RS35750: RR42_RS35750 - DNA-directed RNA polymerase sigma-70 factor, at 3,467,639 to 3,468,355 _RS35750 Position (kb) 3466 3467Strain fitness (log2 ratio) -2 -1 0 1 2at 3465.591 kb on + strand, within RR42_RS35730at 3465.592 kb on - strand, within RR42_RS35730at 3465.624 kb on + strand, within RR42_RS35730at 3465.625 kb on - strand, within RR42_RS35730at 3465.627 kb on + strand, within RR42_RS35730at 3465.627 kb on + strand, within RR42_RS35730at 3465.627 kb on + strand, within RR42_RS35730at 3465.627 kb on + strand, within RR42_RS35730at 3465.628 kb on - strand, within RR42_RS35730at 3465.628 kb on - strand, within RR42_RS35730at 3465.628 kb on - strand, within RR42_RS35730at 3466.035 kb on + strandat 3466.036 kb on - strandat 3466.055 kb on + strandat 3466.055 kb on + strandat 3466.055 kb on + strandat 3466.055 kb on + strandat 3466.108 kb on - strandat 3466.108 kb on - strandat 3466.132 kb on - strandat 3466.480 kb on + strandat 3466.507 kb on - strandat 3466.511 kb on + strandat 3466.512 kb on - strandat 3466.586 kb on - strand, within RR42_RS35740at 3466.611 kb on - strand, within RR42_RS35740at 3466.611 kb on - strand, within RR42_RS35740at 3466.921 kb on - strand, within RR42_RS35740at 3467.177 kb on + strandat 3467.361 kb on + strand, within RR42_RS35745at 3467.362 kb on - strand, within RR42_RS35745at 3467.382 kb on + strand, within RR42_RS35745at 3467.382 kb on + strand, within RR42_RS35745at 3467.383 kb on - strand, within RR42_RS35745at 3467.383 kb on - strand, within RR42_RS35745at 3467.383 kb on - strand, within RR42_RS35745at 3467.383 kb on - strand, within RR42_RS35745at 3467.470 kb on + strand, within RR42_RS35745at 3467.552 kb on + strand, within RR42_RS35745at 3467.552 kb on + strand, within RR42_RS35745at 3467.552 kb on + strand, within RR42_RS35745at 3467.552 kb on + strand, within RR42_RS35745at 3467.552 kb on + strand, within RR42_RS35745at 3467.553 kb on - strand, within RR42_RS35745at 3467.761 kb on - strand, within RR42_RS35750at 3467.774 kb on + strand, within RR42_RS35750at 3467.928 kb on + strand, within RR42_RS35750at 3467.928 kb on + strand, within RR42_RS35750at 3467.928 kb on + strand, within RR42_RS35750at 3467.928 kb on + strand, within RR42_RS35750at 3467.929 kb on - strand, within RR42_RS35750at 3467.929 kb on - strand, within RR42_RS35750at 3467.929 kb on - strand, within RR42_RS35750at 3467.950 kb on - strand, within RR42_RS35750at 3467.950 kb on - strand, within RR42_RS35750at 3467.950 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Lactate (C)
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3,465,591 + RR42_RS35730 0.44 -0.3
3,465,592 - RR42_RS35730 0.44 +0.9
3,465,624 + RR42_RS35730 0.48 +0.0
3,465,625 - RR42_RS35730 0.48 -1.0
3,465,627 + RR42_RS35730 0.49 +1.0
3,465,627 + RR42_RS35730 0.49 +0.0
3,465,627 + RR42_RS35730 0.49 -0.0
3,465,627 + RR42_RS35730 0.49 +1.5
3,465,628 - RR42_RS35730 0.49 -0.5
3,465,628 - RR42_RS35730 0.49 +0.9
3,465,628 - RR42_RS35730 0.49 -1.1
3,466,035 + -0.7
3,466,036 - +0.7
3,466,055 + +2.0
3,466,055 + -0.3
3,466,055 + -1.3
3,466,055 + +0.1
3,466,108 - -0.6
3,466,108 - +0.2
3,466,132 - +0.0
3,466,480 + -0.3
3,466,507 - -2.0
3,466,511 + -1.6
3,466,512 - +0.1
3,466,586 - RR42_RS35740 0.14 +0.4
3,466,611 - RR42_RS35740 0.20 -1.3
3,466,611 - RR42_RS35740 0.20 +1.1
3,466,921 - RR42_RS35740 0.87 +0.8
3,467,177 + -0.3
3,467,361 + RR42_RS35745 0.35 -1.4
3,467,362 - RR42_RS35745 0.35 +1.3
3,467,382 + RR42_RS35745 0.40 +0.0
3,467,382 + RR42_RS35745 0.40 -0.5
3,467,383 - RR42_RS35745 0.40 +0.9
3,467,383 - RR42_RS35745 0.40 -0.7
3,467,383 - RR42_RS35745 0.40 +0.1
3,467,383 - RR42_RS35745 0.40 -0.1
3,467,470 + RR42_RS35745 0.60 +1.0
3,467,552 + RR42_RS35745 0.79 -0.9
3,467,552 + RR42_RS35745 0.79 -0.5
3,467,552 + RR42_RS35745 0.79 -0.6
3,467,552 + RR42_RS35745 0.79 -0.1
3,467,552 + RR42_RS35745 0.79 +0.2
3,467,553 - RR42_RS35745 0.79 +0.8
3,467,761 - RR42_RS35750 0.17 +0.0
3,467,774 + RR42_RS35750 0.19 -0.2
3,467,928 + RR42_RS35750 0.40 -0.2
3,467,928 + RR42_RS35750 0.40 -2.1
3,467,928 + RR42_RS35750 0.40 +0.8
3,467,928 + RR42_RS35750 0.40 -0.4
3,467,929 - RR42_RS35750 0.40 +0.1
3,467,929 - RR42_RS35750 0.40 -0.1
3,467,929 - RR42_RS35750 0.40 -0.6
3,467,950 - RR42_RS35750 0.43 +0.0
3,467,950 - RR42_RS35750 0.43 -0.1
3,467,950 - +1.0

Or see this region's nucleotide sequence