Experiment: octanoate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3996 and Pf6N2E2_3997 are separated by 190 nucleotides Pf6N2E2_3997 and Pf6N2E2_3998 are separated by 88 nucleotides Pf6N2E2_3998 and Pf6N2E2_3999 are separated by 60 nucleotides
Pf6N2E2_3996 - Transcriptional regulator, AraC family, at 4,666,212 to 4,667,216
_3996
Pf6N2E2_3997 - transcriptional regulator, Crp/Fnr family, at 4,667,407 to 4,668,093
_3997
Pf6N2E2_3998 - FIG028593: membrane protein, at 4,668,182 to 4,668,706
_3998
Pf6N2E2_3999 - Glucose-methanol-choline (GMC) oxidoreductase:NAD binding site, at 4,668,767 to 4,670,989
_3999
Position (kb)
4667
4668
4669 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4666.651 kb on - strand, within Pf6N2E2_3996 at 4666.651 kb on - strand, within Pf6N2E2_3996 at 4666.651 kb on - strand, within Pf6N2E2_3996 at 4666.687 kb on - strand, within Pf6N2E2_3996 at 4666.704 kb on + strand, within Pf6N2E2_3996 at 4666.705 kb on - strand, within Pf6N2E2_3996 at 4666.878 kb on - strand, within Pf6N2E2_3996 at 4666.919 kb on - strand, within Pf6N2E2_3996 at 4666.919 kb on - strand, within Pf6N2E2_3996 at 4667.121 kb on + strand at 4667.122 kb on - strand at 4667.122 kb on - strand at 4667.335 kb on + strand at 4667.337 kb on + strand at 4667.337 kb on + strand at 4667.337 kb on + strand at 4667.337 kb on + strand at 4667.338 kb on - strand at 4667.724 kb on - strand, within Pf6N2E2_3997 at 4667.868 kb on + strand, within Pf6N2E2_3997 at 4667.868 kb on + strand, within Pf6N2E2_3997 at 4667.869 kb on - strand, within Pf6N2E2_3997 at 4667.936 kb on + strand, within Pf6N2E2_3997 at 4667.937 kb on - strand, within Pf6N2E2_3997 at 4667.937 kb on - strand, within Pf6N2E2_3997 at 4667.937 kb on - strand, within Pf6N2E2_3997 at 4668.019 kb on - strand, within Pf6N2E2_3997 at 4668.019 kb on - strand, within Pf6N2E2_3997 at 4668.019 kb on - strand, within Pf6N2E2_3997 at 4668.019 kb on - strand, within Pf6N2E2_3997 at 4668.019 kb on - strand, within Pf6N2E2_3997 at 4668.025 kb on + strand at 4668.025 kb on + strand at 4668.026 kb on - strand at 4668.026 kb on - strand at 4668.026 kb on - strand at 4668.047 kb on + strand at 4668.048 kb on - strand at 4668.048 kb on - strand at 4668.048 kb on - strand at 4668.212 kb on + strand at 4668.213 kb on - strand at 4668.221 kb on + strand at 4668.222 kb on - strand at 4668.374 kb on + strand, within Pf6N2E2_3998 at 4668.374 kb on + strand, within Pf6N2E2_3998 at 4668.374 kb on + strand, within Pf6N2E2_3998 at 4668.375 kb on - strand, within Pf6N2E2_3998 at 4668.375 kb on - strand, within Pf6N2E2_3998 at 4668.378 kb on + strand, within Pf6N2E2_3998 at 4668.378 kb on + strand, within Pf6N2E2_3998 at 4668.378 kb on + strand, within Pf6N2E2_3998 at 4668.379 kb on - strand at 4668.379 kb on - strand, within Pf6N2E2_3998 at 4668.533 kb on + strand, within Pf6N2E2_3998 at 4668.536 kb on + strand, within Pf6N2E2_3998 at 4668.536 kb on + strand, within Pf6N2E2_3998 at 4668.536 kb on + strand, within Pf6N2E2_3998 at 4668.536 kb on + strand, within Pf6N2E2_3998 at 4668.537 kb on - strand, within Pf6N2E2_3998 at 4668.537 kb on - strand, within Pf6N2E2_3998 at 4668.537 kb on - strand, within Pf6N2E2_3998 at 4668.537 kb on - strand, within Pf6N2E2_3998 at 4668.621 kb on + strand, within Pf6N2E2_3998 at 4668.769 kb on - strand at 4668.811 kb on + strand at 4668.978 kb on + strand at 4668.978 kb on + strand at 4668.979 kb on - strand at 4668.979 kb on - strand at 4668.979 kb on - strand at 4668.979 kb on - strand at 4668.979 kb on - strand at 4668.979 kb on - strand at 4668.979 kb on - strand at 4668.979 kb on - strand at 4669.049 kb on + strand, within Pf6N2E2_3999 at 4669.050 kb on - strand, within Pf6N2E2_3999
Per-strain Table
Position Strand Gene LocusTag Fraction octanoate (C) remove 4,666,651 - Pf6N2E2_3996 0.44 -0.4 4,666,651 - Pf6N2E2_3996 0.44 -0.5 4,666,651 - Pf6N2E2_3996 0.44 +0.7 4,666,687 - Pf6N2E2_3996 0.47 -1.2 4,666,704 + Pf6N2E2_3996 0.49 +0.6 4,666,705 - Pf6N2E2_3996 0.49 -1.0 4,666,878 - Pf6N2E2_3996 0.66 -1.6 4,666,919 - Pf6N2E2_3996 0.70 -0.2 4,666,919 - Pf6N2E2_3996 0.70 -0.1 4,667,121 + -0.1 4,667,122 - -0.7 4,667,122 - +0.5 4,667,335 + +0.9 4,667,337 + +0.7 4,667,337 + -1.2 4,667,337 + -0.6 4,667,337 + +0.7 4,667,338 - +0.6 4,667,724 - Pf6N2E2_3997 0.46 +1.0 4,667,868 + Pf6N2E2_3997 0.67 -2.7 4,667,868 + Pf6N2E2_3997 0.67 +2.8 4,667,869 - Pf6N2E2_3997 0.67 +1.4 4,667,936 + Pf6N2E2_3997 0.77 +1.1 4,667,937 - Pf6N2E2_3997 0.77 +2.0 4,667,937 - Pf6N2E2_3997 0.77 -1.8 4,667,937 - Pf6N2E2_3997 0.77 +0.2 4,668,019 - Pf6N2E2_3997 0.89 +0.5 4,668,019 - Pf6N2E2_3997 0.89 +0.0 4,668,019 - Pf6N2E2_3997 0.89 +0.9 4,668,019 - Pf6N2E2_3997 0.89 -0.7 4,668,019 - Pf6N2E2_3997 0.89 -0.3 4,668,025 + -0.3 4,668,025 + -3.3 4,668,026 - +2.6 4,668,026 - -0.3 4,668,026 - -0.2 4,668,047 + -0.4 4,668,048 - -0.4 4,668,048 - -0.9 4,668,048 - +0.6 4,668,212 + +0.6 4,668,213 - -0.7 4,668,221 + +0.2 4,668,222 - -0.2 4,668,374 + Pf6N2E2_3998 0.37 -3.4 4,668,374 + Pf6N2E2_3998 0.37 +0.9 4,668,374 + Pf6N2E2_3998 0.37 -0.7 4,668,375 - Pf6N2E2_3998 0.37 +0.2 4,668,375 - Pf6N2E2_3998 0.37 +0.9 4,668,378 + Pf6N2E2_3998 0.37 -2.8 4,668,378 + Pf6N2E2_3998 0.37 +0.8 4,668,378 + Pf6N2E2_3998 0.37 +0.7 4,668,379 - +2.0 4,668,379 - Pf6N2E2_3998 0.38 +0.9 4,668,533 + Pf6N2E2_3998 0.67 +0.2 4,668,536 + Pf6N2E2_3998 0.67 +0.5 4,668,536 + Pf6N2E2_3998 0.67 +0.4 4,668,536 + Pf6N2E2_3998 0.67 +0.0 4,668,536 + Pf6N2E2_3998 0.67 +0.7 4,668,537 - Pf6N2E2_3998 0.68 -0.6 4,668,537 - Pf6N2E2_3998 0.68 +0.4 4,668,537 - Pf6N2E2_3998 0.68 +1.2 4,668,537 - Pf6N2E2_3998 0.68 -0.1 4,668,621 + Pf6N2E2_3998 0.84 -0.3 4,668,769 - +0.7 4,668,811 + -0.6 4,668,978 + +1.5 4,668,978 + +0.9 4,668,979 - -0.6 4,668,979 - +0.6 4,668,979 - +0.8 4,668,979 - -0.1 4,668,979 - -1.1 4,668,979 - +0.3 4,668,979 - -3.7 4,668,979 - +0.8 4,669,049 + Pf6N2E2_3999 0.13 -0.4 4,669,050 - Pf6N2E2_3999 0.13 -1.5
Or see this region's nucleotide sequence