Experiment: octanoate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1481 and Pf6N2E2_1482 are separated by 152 nucleotides Pf6N2E2_1482 and Pf6N2E2_1483 are separated by 192 nucleotides Pf6N2E2_1483 and Pf6N2E2_1484 are separated by 28 nucleotides
Pf6N2E2_1481 - VgrG protein, at 1,730,306 to 1,731,634
_1481
Pf6N2E2_1482 - Probable periplasmic spermidine/putrescine-binding protein, at 1,731,787 to 1,732,890
_1482
Pf6N2E2_1483 - transcriptional regulator, LuxR family, at 1,733,083 to 1,733,820
_1483
Pf6N2E2_1484 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 1,733,849 to 1,734,778
_1484
Position (kb)
1731
1732
1733 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1730.858 kb on - strand, within Pf6N2E2_1481 at 1730.871 kb on - strand, within Pf6N2E2_1481 at 1730.897 kb on - strand, within Pf6N2E2_1481 at 1730.899 kb on - strand, within Pf6N2E2_1481 at 1730.949 kb on + strand, within Pf6N2E2_1481 at 1730.950 kb on - strand, within Pf6N2E2_1481 at 1730.950 kb on - strand, within Pf6N2E2_1481 at 1731.189 kb on + strand, within Pf6N2E2_1481 at 1731.201 kb on + strand, within Pf6N2E2_1481 at 1731.216 kb on + strand, within Pf6N2E2_1481 at 1731.310 kb on - strand, within Pf6N2E2_1481 at 1731.310 kb on - strand, within Pf6N2E2_1481 at 1731.340 kb on - strand, within Pf6N2E2_1481 at 1731.411 kb on + strand at 1731.528 kb on + strand at 1731.529 kb on - strand at 1731.650 kb on + strand at 1731.654 kb on + strand at 1731.655 kb on - strand at 1731.740 kb on - strand at 1731.772 kb on - strand at 1731.864 kb on + strand at 1731.865 kb on - strand at 1732.007 kb on + strand, within Pf6N2E2_1482 at 1732.188 kb on + strand, within Pf6N2E2_1482 at 1732.189 kb on - strand, within Pf6N2E2_1482 at 1732.241 kb on + strand, within Pf6N2E2_1482 at 1732.242 kb on - strand, within Pf6N2E2_1482 at 1732.280 kb on + strand, within Pf6N2E2_1482 at 1732.296 kb on + strand, within Pf6N2E2_1482 at 1732.297 kb on - strand, within Pf6N2E2_1482 at 1732.472 kb on + strand, within Pf6N2E2_1482 at 1732.495 kb on + strand, within Pf6N2E2_1482 at 1732.496 kb on - strand, within Pf6N2E2_1482 at 1732.500 kb on - strand, within Pf6N2E2_1482 at 1732.588 kb on - strand, within Pf6N2E2_1482 at 1732.791 kb on + strand at 1732.792 kb on - strand at 1732.792 kb on - strand at 1732.792 kb on - strand at 1732.793 kb on + strand at 1732.793 kb on + strand at 1732.800 kb on - strand at 1732.800 kb on - strand at 1732.800 kb on - strand at 1732.800 kb on - strand at 1732.834 kb on + strand at 1732.948 kb on + strand at 1732.951 kb on - strand at 1732.968 kb on - strand at 1732.970 kb on + strand at 1732.971 kb on - strand at 1732.984 kb on + strand at 1732.984 kb on + strand at 1732.984 kb on + strand at 1732.985 kb on - strand at 1732.989 kb on - strand at 1733.015 kb on + strand at 1733.487 kb on - strand, within Pf6N2E2_1483 at 1733.700 kb on - strand, within Pf6N2E2_1483
Per-strain Table
Position Strand Gene LocusTag Fraction octanoate (C) remove 1,730,858 - Pf6N2E2_1481 0.42 -0.3 1,730,871 - Pf6N2E2_1481 0.43 -2.0 1,730,897 - Pf6N2E2_1481 0.44 +0.2 1,730,899 - Pf6N2E2_1481 0.45 +1.0 1,730,949 + Pf6N2E2_1481 0.48 +2.2 1,730,950 - Pf6N2E2_1481 0.48 -1.4 1,730,950 - Pf6N2E2_1481 0.48 +0.9 1,731,189 + Pf6N2E2_1481 0.66 -0.1 1,731,201 + Pf6N2E2_1481 0.67 -0.2 1,731,216 + Pf6N2E2_1481 0.68 +0.3 1,731,310 - Pf6N2E2_1481 0.76 -0.4 1,731,310 - Pf6N2E2_1481 0.76 -1.1 1,731,340 - Pf6N2E2_1481 0.78 -2.7 1,731,411 + -1.6 1,731,528 + -0.4 1,731,529 - -0.0 1,731,650 + -3.4 1,731,654 + -2.4 1,731,655 - -3.3 1,731,740 - -0.6 1,731,772 - -0.7 1,731,864 + -3.9 1,731,865 - -3.4 1,732,007 + Pf6N2E2_1482 0.20 -3.3 1,732,188 + Pf6N2E2_1482 0.36 -0.7 1,732,189 - Pf6N2E2_1482 0.36 -2.9 1,732,241 + Pf6N2E2_1482 0.41 -0.8 1,732,242 - Pf6N2E2_1482 0.41 -0.1 1,732,280 + Pf6N2E2_1482 0.45 -1.8 1,732,296 + Pf6N2E2_1482 0.46 -1.0 1,732,297 - Pf6N2E2_1482 0.46 -3.9 1,732,472 + Pf6N2E2_1482 0.62 -3.1 1,732,495 + Pf6N2E2_1482 0.64 -2.1 1,732,496 - Pf6N2E2_1482 0.64 -0.9 1,732,500 - Pf6N2E2_1482 0.65 -0.8 1,732,588 - Pf6N2E2_1482 0.73 -3.3 1,732,791 + -3.2 1,732,792 - +2.0 1,732,792 - -1.8 1,732,792 - -0.6 1,732,793 + -1.0 1,732,793 + -0.8 1,732,800 - -1.6 1,732,800 - -1.0 1,732,800 - -1.1 1,732,800 - -1.7 1,732,834 + -2.6 1,732,948 + -0.7 1,732,951 - -0.4 1,732,968 - -0.0 1,732,970 + +0.6 1,732,971 - -0.9 1,732,984 + -1.1 1,732,984 + -1.4 1,732,984 + +0.5 1,732,985 - -0.5 1,732,989 - +0.1 1,733,015 + -1.0 1,733,487 - Pf6N2E2_1483 0.55 -2.4 1,733,700 - Pf6N2E2_1483 0.84 +0.4
Or see this region's nucleotide sequence