Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_6035 and Pf1N1B4_6036 are separated by 179 nucleotides Pf1N1B4_6036 and Pf1N1B4_6037 are separated by 336 nucleotides Pf1N1B4_6037 and Pf1N1B4_6038 are separated by 213 nucleotides Pf1N1B4_6038 and Pf1N1B4_6039 are separated by 4 nucleotides
Pf1N1B4_6035 - D-ribose ABC transporter, substrate-binding component RbsB (from data) , at 499,543 to 500,499
_6035
Pf1N1B4_6036 - FIG00953132: hypothetical protein, at 500,679 to 501,131
_6036
Pf1N1B4_6037 - L-asparaginase (EC 3.5.1.1), at 501,468 to 502,556
_6037
Pf1N1B4_6038 - FIG00953874: hypothetical protein, at 502,770 to 503,009
_6038
Pf1N1B4_6039 - Endonuclease I precursor (EC 3.1.21.1), at 503,014 to 503,703
_6039
Position (kb)
501
502
503 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 500.516 kb on + strand at 500.608 kb on + strand at 500.608 kb on + strand at 500.608 kb on + strand at 500.609 kb on - strand at 500.609 kb on - strand at 500.609 kb on - strand at 500.675 kb on + strand at 500.676 kb on - strand at 500.676 kb on - strand at 500.676 kb on - strand at 500.910 kb on + strand, within Pf1N1B4_6036 at 500.925 kb on - strand, within Pf1N1B4_6036 at 500.925 kb on - strand, within Pf1N1B4_6036 at 500.925 kb on - strand, within Pf1N1B4_6036 at 500.925 kb on - strand at 501.002 kb on + strand, within Pf1N1B4_6036 at 501.003 kb on - strand, within Pf1N1B4_6036 at 501.065 kb on + strand at 501.122 kb on + strand at 501.131 kb on + strand at 501.131 kb on + strand at 501.131 kb on + strand at 501.131 kb on + strand at 501.131 kb on + strand at 501.131 kb on + strand at 501.131 kb on + strand at 501.131 kb on + strand at 501.131 kb on + strand at 501.131 kb on + strand at 501.131 kb on + strand at 501.132 kb on - strand at 501.132 kb on - strand at 501.132 kb on - strand at 501.132 kb on - strand at 501.132 kb on - strand at 501.132 kb on - strand at 501.157 kb on - strand at 501.248 kb on - strand at 501.255 kb on - strand at 501.344 kb on + strand at 501.385 kb on + strand at 501.385 kb on + strand at 501.386 kb on - strand at 501.422 kb on + strand at 501.422 kb on + strand at 501.452 kb on - strand at 501.467 kb on - strand at 501.514 kb on + strand at 501.864 kb on + strand, within Pf1N1B4_6037 at 501.865 kb on - strand at 501.865 kb on - strand, within Pf1N1B4_6037 at 501.911 kb on - strand, within Pf1N1B4_6037 at 502.230 kb on + strand, within Pf1N1B4_6037 at 502.255 kb on - strand at 502.366 kb on + strand, within Pf1N1B4_6037 at 502.366 kb on + strand, within Pf1N1B4_6037 at 502.408 kb on + strand at 502.440 kb on + strand, within Pf1N1B4_6037 at 502.440 kb on + strand, within Pf1N1B4_6037 at 502.441 kb on - strand, within Pf1N1B4_6037 at 502.551 kb on + strand at 502.551 kb on + strand at 502.551 kb on + strand at 502.552 kb on - strand at 502.660 kb on - strand at 502.705 kb on - strand at 502.734 kb on + strand at 502.764 kb on - strand at 502.796 kb on + strand at 502.796 kb on + strand, within Pf1N1B4_6038 at 502.800 kb on + strand, within Pf1N1B4_6038 at 502.800 kb on + strand, within Pf1N1B4_6038 at 502.800 kb on + strand, within Pf1N1B4_6038 at 502.801 kb on - strand, within Pf1N1B4_6038 at 502.801 kb on - strand, within Pf1N1B4_6038 at 502.801 kb on - strand, within Pf1N1B4_6038 at 502.801 kb on - strand, within Pf1N1B4_6038 at 503.036 kb on + strand at 503.119 kb on + strand, within Pf1N1B4_6039 at 503.144 kb on - strand at 503.158 kb on + strand, within Pf1N1B4_6039 at 503.158 kb on + strand, within Pf1N1B4_6039 at 503.159 kb on - strand at 503.195 kb on - strand at 503.195 kb on - strand, within Pf1N1B4_6039 at 503.329 kb on + strand, within Pf1N1B4_6039 at 503.330 kb on - strand, within Pf1N1B4_6039 at 503.330 kb on - strand at 503.437 kb on + strand, within Pf1N1B4_6039 at 503.521 kb on + strand, within Pf1N1B4_6039 at 503.522 kb on - strand, within Pf1N1B4_6039 at 503.522 kb on - strand, within Pf1N1B4_6039 at 503.522 kb on - strand, within Pf1N1B4_6039 at 503.522 kb on - strand, within Pf1N1B4_6039 at 503.527 kb on + strand, within Pf1N1B4_6039 at 503.528 kb on - strand, within Pf1N1B4_6039 at 503.528 kb on - strand, within Pf1N1B4_6039 at 503.528 kb on - strand, within Pf1N1B4_6039 at 503.535 kb on + strand, within Pf1N1B4_6039 at 503.535 kb on + strand, within Pf1N1B4_6039
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 500,516 + -1.0 500,608 + -0.0 500,608 + +0.1 500,608 + -0.0 500,609 - -0.0 500,609 - +1.1 500,609 - -1.0 500,675 + +1.3 500,676 - -0.7 500,676 - -1.0 500,676 - +0.1 500,910 + Pf1N1B4_6036 0.51 +0.3 500,925 - Pf1N1B4_6036 0.54 -1.6 500,925 - Pf1N1B4_6036 0.54 -1.0 500,925 - Pf1N1B4_6036 0.54 -1.0 500,925 - -1.0 501,002 + Pf1N1B4_6036 0.71 -1.6 501,003 - Pf1N1B4_6036 0.72 -0.3 501,065 + -1.5 501,122 + +0.5 501,131 + -0.5 501,131 + -1.2 501,131 + -2.0 501,131 + -2.3 501,131 + -1.8 501,131 + -0.1 501,131 + +1.5 501,131 + -0.5 501,131 + -0.1 501,131 + -0.5 501,131 + +0.9 501,132 - -0.5 501,132 - -0.3 501,132 - -0.3 501,132 - -0.7 501,132 - -1.0 501,132 - +0.1 501,157 - -1.5 501,248 - +1.5 501,255 - +0.5 501,344 + +1.0 501,385 + +2.5 501,385 + -0.5 501,386 - -1.0 501,422 + -0.2 501,422 + +0.1 501,452 - +0.9 501,467 - -1.9 501,514 + +1.9 501,864 + Pf1N1B4_6037 0.36 +1.0 501,865 - +0.5 501,865 - Pf1N1B4_6037 0.36 -0.7 501,911 - Pf1N1B4_6037 0.41 -0.8 502,230 + Pf1N1B4_6037 0.70 +0.1 502,255 - -1.5 502,366 + Pf1N1B4_6037 0.82 -1.8 502,366 + Pf1N1B4_6037 0.82 -1.0 502,408 + +0.5 502,440 + Pf1N1B4_6037 0.89 -1.0 502,440 + Pf1N1B4_6037 0.89 -1.5 502,441 - Pf1N1B4_6037 0.89 +0.1 502,551 + -0.5 502,551 + +0.5 502,551 + -1.3 502,552 - -0.9 502,660 - -1.5 502,705 - -2.2 502,734 + -1.3 502,764 - -0.8 502,796 + +0.5 502,796 + Pf1N1B4_6038 0.11 -0.9 502,800 + Pf1N1B4_6038 0.12 -1.8 502,800 + Pf1N1B4_6038 0.12 -0.3 502,800 + Pf1N1B4_6038 0.12 -1.0 502,801 - Pf1N1B4_6038 0.13 -0.9 502,801 - Pf1N1B4_6038 0.13 -1.9 502,801 - Pf1N1B4_6038 0.13 -0.3 502,801 - Pf1N1B4_6038 0.13 -0.3 503,036 + -0.3 503,119 + Pf1N1B4_6039 0.15 +2.9 503,144 - +0.5 503,158 + Pf1N1B4_6039 0.21 -0.5 503,158 + Pf1N1B4_6039 0.21 -0.3 503,159 - +0.5 503,195 - -1.0 503,195 - Pf1N1B4_6039 0.26 -3.2 503,329 + Pf1N1B4_6039 0.46 -0.8 503,330 - Pf1N1B4_6039 0.46 -0.1 503,330 - -0.5 503,437 + Pf1N1B4_6039 0.61 -1.2 503,521 + Pf1N1B4_6039 0.73 +0.0 503,522 - Pf1N1B4_6039 0.74 -0.6 503,522 - Pf1N1B4_6039 0.74 -2.0 503,522 - Pf1N1B4_6039 0.74 -1.5 503,522 - Pf1N1B4_6039 0.74 -0.1 503,527 + Pf1N1B4_6039 0.74 +0.5 503,528 - Pf1N1B4_6039 0.74 -1.0 503,528 - Pf1N1B4_6039 0.74 +0.3 503,528 - Pf1N1B4_6039 0.74 -0.1 503,535 + Pf1N1B4_6039 0.76 -1.2 503,535 + Pf1N1B4_6039 0.76 -2.3
Or see this region's nucleotide sequence