Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2973 and Pf1N1B4_2974 overlap by 4 nucleotides Pf1N1B4_2974 and Pf1N1B4_2975 are separated by 154 nucleotides Pf1N1B4_2975 and Pf1N1B4_2976 are separated by 43 nucleotides
Pf1N1B4_2973 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 3,334,257 to 3,334,721
_2973
Pf1N1B4_2974 - Phosphate regulon transcriptional regulatory protein PhoB (SphR), at 3,334,718 to 3,335,434
_2974
Pf1N1B4_2975 - Peptide methionine sulfoxide reductase MsrB (EC 1.8.4.12), at 3,335,589 to 3,336,104
_2975
Pf1N1B4_2976 - DipZ protein, at 3,336,148 to 3,337,935
_2976
Position (kb)
3335
3336
3337 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3334.750 kb on - strand at 3334.849 kb on - strand, within Pf1N1B4_2974 at 3334.929 kb on + strand, within Pf1N1B4_2974 at 3334.929 kb on + strand, within Pf1N1B4_2974 at 3334.929 kb on + strand, within Pf1N1B4_2974 at 3334.930 kb on - strand, within Pf1N1B4_2974 at 3334.930 kb on - strand, within Pf1N1B4_2974 at 3334.930 kb on - strand, within Pf1N1B4_2974 at 3334.930 kb on - strand, within Pf1N1B4_2974 at 3334.930 kb on - strand, within Pf1N1B4_2974 at 3335.130 kb on + strand, within Pf1N1B4_2974 at 3335.378 kb on - strand at 3335.494 kb on + strand at 3335.494 kb on + strand at 3335.495 kb on - strand at 3335.508 kb on + strand at 3335.544 kb on + strand at 3335.544 kb on + strand at 3335.545 kb on - strand at 3335.545 kb on - strand at 3335.656 kb on + strand, within Pf1N1B4_2975 at 3335.657 kb on - strand, within Pf1N1B4_2975 at 3335.657 kb on - strand, within Pf1N1B4_2975 at 3335.806 kb on - strand at 3335.806 kb on - strand, within Pf1N1B4_2975 at 3335.834 kb on + strand, within Pf1N1B4_2975 at 3335.835 kb on - strand, within Pf1N1B4_2975 at 3336.060 kb on + strand at 3336.102 kb on + strand at 3336.147 kb on + strand at 3336.147 kb on + strand at 3336.147 kb on + strand at 3336.147 kb on + strand at 3336.147 kb on + strand at 3336.147 kb on + strand at 3336.148 kb on - strand at 3336.148 kb on - strand at 3336.169 kb on + strand at 3336.170 kb on - strand at 3336.356 kb on - strand, within Pf1N1B4_2976 at 3336.768 kb on - strand, within Pf1N1B4_2976 at 3336.867 kb on + strand, within Pf1N1B4_2976 at 3337.081 kb on + strand, within Pf1N1B4_2976 at 3337.081 kb on + strand, within Pf1N1B4_2976 at 3337.081 kb on + strand, within Pf1N1B4_2976 at 3337.081 kb on + strand, within Pf1N1B4_2976 at 3337.082 kb on - strand, within Pf1N1B4_2976 at 3337.082 kb on - strand, within Pf1N1B4_2976
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 3,334,750 - -0.1 3,334,849 - Pf1N1B4_2974 0.18 +3.2 3,334,929 + Pf1N1B4_2974 0.29 +0.3 3,334,929 + Pf1N1B4_2974 0.29 +0.6 3,334,929 + Pf1N1B4_2974 0.29 +0.3 3,334,930 - Pf1N1B4_2974 0.30 +0.6 3,334,930 - Pf1N1B4_2974 0.30 +0.8 3,334,930 - Pf1N1B4_2974 0.30 +1.2 3,334,930 - Pf1N1B4_2974 0.30 -0.5 3,334,930 - Pf1N1B4_2974 0.30 +0.4 3,335,130 + Pf1N1B4_2974 0.57 -0.9 3,335,378 - -0.5 3,335,494 + -1.1 3,335,494 + -0.0 3,335,495 - +1.2 3,335,508 + +0.3 3,335,544 + -0.2 3,335,544 + +0.1 3,335,545 - -0.3 3,335,545 - +1.5 3,335,656 + Pf1N1B4_2975 0.13 -1.1 3,335,657 - Pf1N1B4_2975 0.13 -0.4 3,335,657 - Pf1N1B4_2975 0.13 +0.2 3,335,806 - -0.4 3,335,806 - Pf1N1B4_2975 0.42 -1.1 3,335,834 + Pf1N1B4_2975 0.47 -1.1 3,335,835 - Pf1N1B4_2975 0.48 -0.1 3,336,060 + -0.2 3,336,102 + +2.2 3,336,147 + -0.5 3,336,147 + +0.2 3,336,147 + -1.1 3,336,147 + -1.4 3,336,147 + -0.1 3,336,147 + -0.2 3,336,148 - -0.1 3,336,148 - +0.7 3,336,169 + -0.2 3,336,170 - -0.5 3,336,356 - Pf1N1B4_2976 0.12 +0.5 3,336,768 - Pf1N1B4_2976 0.35 +0.9 3,336,867 + Pf1N1B4_2976 0.40 -0.1 3,337,081 + Pf1N1B4_2976 0.52 -0.9 3,337,081 + Pf1N1B4_2976 0.52 -0.5 3,337,081 + Pf1N1B4_2976 0.52 -0.7 3,337,081 + Pf1N1B4_2976 0.52 -0.4 3,337,082 - Pf1N1B4_2976 0.52 -0.4 3,337,082 - Pf1N1B4_2976 0.52 -0.0
Or see this region's nucleotide sequence