Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1476 and Pf1N1B4_1477 are separated by 11 nucleotides Pf1N1B4_1477 and Pf1N1B4_1478 are separated by 283 nucleotides
Pf1N1B4_1476 - Pyruvate dehydrogenase E1 component (EC 1.2.4.1), at 1,692,445 to 1,695,090
_1476
Pf1N1B4_1477 - Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex (EC 2.3.1.12), at 1,695,102 to 1,697,069
_1477
Pf1N1B4_1478 - Phosphodiesterase/alkaline phosphatase D-like, at 1,697,353 to 1,698,747
_1478
Position (kb)
1695
1696
1697
1698 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1694.209 kb on + strand, within Pf1N1B4_1476 at 1694.219 kb on - strand, within Pf1N1B4_1476 at 1694.482 kb on + strand, within Pf1N1B4_1476 at 1697.067 kb on + strand at 1697.067 kb on + strand at 1697.068 kb on - strand at 1697.068 kb on - strand at 1697.197 kb on + strand at 1697.198 kb on - strand at 1697.198 kb on - strand at 1697.216 kb on + strand at 1697.217 kb on - strand at 1697.226 kb on - strand at 1697.272 kb on + strand at 1697.272 kb on + strand at 1697.272 kb on + strand at 1697.278 kb on + strand at 1697.278 kb on + strand at 1697.282 kb on + strand at 1697.323 kb on + strand at 1697.324 kb on - strand at 1697.324 kb on - strand at 1697.327 kb on + strand at 1697.328 kb on - strand at 1697.333 kb on + strand at 1697.333 kb on + strand at 1697.334 kb on - strand at 1697.334 kb on - strand at 1697.398 kb on - strand at 1697.398 kb on - strand at 1697.398 kb on - strand at 1697.448 kb on - strand at 1697.501 kb on + strand, within Pf1N1B4_1478 at 1697.501 kb on + strand, within Pf1N1B4_1478 at 1697.502 kb on - strand, within Pf1N1B4_1478 at 1697.576 kb on - strand, within Pf1N1B4_1478 at 1697.576 kb on - strand, within Pf1N1B4_1478 at 1697.668 kb on + strand, within Pf1N1B4_1478 at 1697.713 kb on + strand, within Pf1N1B4_1478 at 1697.713 kb on + strand, within Pf1N1B4_1478 at 1697.713 kb on + strand, within Pf1N1B4_1478 at 1697.714 kb on - strand, within Pf1N1B4_1478 at 1697.714 kb on - strand, within Pf1N1B4_1478 at 1697.714 kb on - strand, within Pf1N1B4_1478 at 1697.714 kb on - strand, within Pf1N1B4_1478 at 1697.714 kb on - strand, within Pf1N1B4_1478 at 1697.714 kb on - strand, within Pf1N1B4_1478 at 1697.714 kb on - strand, within Pf1N1B4_1478 at 1697.714 kb on - strand, within Pf1N1B4_1478 at 1697.714 kb on - strand, within Pf1N1B4_1478 at 1697.714 kb on - strand, within Pf1N1B4_1478 at 1697.714 kb on - strand, within Pf1N1B4_1478 at 1697.714 kb on - strand, within Pf1N1B4_1478 at 1697.745 kb on + strand, within Pf1N1B4_1478 at 1697.864 kb on - strand, within Pf1N1B4_1478 at 1697.864 kb on - strand, within Pf1N1B4_1478 at 1697.864 kb on - strand, within Pf1N1B4_1478 at 1697.864 kb on - strand, within Pf1N1B4_1478 at 1697.902 kb on + strand, within Pf1N1B4_1478 at 1697.903 kb on - strand, within Pf1N1B4_1478 at 1697.949 kb on + strand, within Pf1N1B4_1478 at 1697.950 kb on - strand, within Pf1N1B4_1478 at 1697.980 kb on + strand, within Pf1N1B4_1478 at 1697.980 kb on + strand, within Pf1N1B4_1478 at 1697.980 kb on + strand, within Pf1N1B4_1478 at 1697.981 kb on - strand, within Pf1N1B4_1478 at 1697.981 kb on - strand, within Pf1N1B4_1478 at 1698.047 kb on - strand, within Pf1N1B4_1478 at 1698.047 kb on - strand, within Pf1N1B4_1478 at 1698.051 kb on - strand, within Pf1N1B4_1478 at 1698.053 kb on - strand, within Pf1N1B4_1478
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 1,694,209 + Pf1N1B4_1476 0.67 -0.6 1,694,219 - Pf1N1B4_1476 0.67 -0.2 1,694,482 + Pf1N1B4_1476 0.77 -2.2 1,697,067 + -3.0 1,697,067 + -1.6 1,697,068 - -1.4 1,697,068 - -1.2 1,697,197 + +0.6 1,697,198 - +1.4 1,697,198 - +0.2 1,697,216 + -0.9 1,697,217 - -1.2 1,697,226 - +1.0 1,697,272 + -0.8 1,697,272 + +0.4 1,697,272 + -0.5 1,697,278 + -0.4 1,697,278 + +0.4 1,697,282 + +1.4 1,697,323 + -0.3 1,697,324 - -1.0 1,697,324 - +0.1 1,697,327 + +0.4 1,697,328 - +0.9 1,697,333 + -0.8 1,697,333 + +1.0 1,697,334 - +0.9 1,697,334 - -0.9 1,697,398 - -2.3 1,697,398 - -0.9 1,697,398 - -0.3 1,697,448 - +1.1 1,697,501 + Pf1N1B4_1478 0.11 +0.4 1,697,501 + Pf1N1B4_1478 0.11 -0.6 1,697,502 - Pf1N1B4_1478 0.11 +2.4 1,697,576 - Pf1N1B4_1478 0.16 +0.6 1,697,576 - Pf1N1B4_1478 0.16 +0.4 1,697,668 + Pf1N1B4_1478 0.23 -0.2 1,697,713 + Pf1N1B4_1478 0.26 +0.6 1,697,713 + Pf1N1B4_1478 0.26 -0.5 1,697,713 + Pf1N1B4_1478 0.26 -0.0 1,697,714 - Pf1N1B4_1478 0.26 -1.8 1,697,714 - Pf1N1B4_1478 0.26 -0.2 1,697,714 - Pf1N1B4_1478 0.26 -1.2 1,697,714 - Pf1N1B4_1478 0.26 -1.8 1,697,714 - Pf1N1B4_1478 0.26 +0.4 1,697,714 - Pf1N1B4_1478 0.26 +0.2 1,697,714 - Pf1N1B4_1478 0.26 -0.5 1,697,714 - Pf1N1B4_1478 0.26 -1.4 1,697,714 - Pf1N1B4_1478 0.26 +0.0 1,697,714 - Pf1N1B4_1478 0.26 -0.9 1,697,714 - Pf1N1B4_1478 0.26 +0.1 1,697,714 - Pf1N1B4_1478 0.26 +1.1 1,697,745 + Pf1N1B4_1478 0.28 +0.1 1,697,864 - Pf1N1B4_1478 0.37 -1.8 1,697,864 - Pf1N1B4_1478 0.37 +0.2 1,697,864 - Pf1N1B4_1478 0.37 +0.4 1,697,864 - Pf1N1B4_1478 0.37 -0.4 1,697,902 + Pf1N1B4_1478 0.39 +0.6 1,697,903 - Pf1N1B4_1478 0.39 +0.2 1,697,949 + Pf1N1B4_1478 0.43 -0.7 1,697,950 - Pf1N1B4_1478 0.43 +0.9 1,697,980 + Pf1N1B4_1478 0.45 +0.1 1,697,980 + Pf1N1B4_1478 0.45 +0.4 1,697,980 + Pf1N1B4_1478 0.45 +0.1 1,697,981 - Pf1N1B4_1478 0.45 +1.1 1,697,981 - Pf1N1B4_1478 0.45 -1.2 1,698,047 - Pf1N1B4_1478 0.50 -0.0 1,698,047 - Pf1N1B4_1478 0.50 +0.4 1,698,051 - Pf1N1B4_1478 0.50 -0.6 1,698,053 - Pf1N1B4_1478 0.50 -0.3
Or see this region's nucleotide sequence