Experiment: D,L-Lactate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1186 and Pf1N1B4_1187 are separated by 151 nucleotides Pf1N1B4_1187 and Pf1N1B4_1188 overlap by 4 nucleotides Pf1N1B4_1188 and Pf1N1B4_1189 overlap by 1 nucleotides Pf1N1B4_1189 and Pf1N1B4_1190 overlap by 4 nucleotides
Pf1N1B4_1186 - L-lactate permease, at 1,336,514 to 1,338,205
_1186
Pf1N1B4_1187 - Predicted L-lactate dehydrogenase, Fe-S oxidoreductase subunit YkgE, at 1,338,357 to 1,339,181
_1187
Pf1N1B4_1188 - Predicted L-lactate dehydrogenase, Iron-sulfur cluster-binding subunit YkgF, at 1,339,178 to 1,340,632
_1188
Pf1N1B4_1189 - Predicted L-lactate dehydrogenase, hypothetical protein subunit YkgG, at 1,340,632 to 1,341,303
_1189
Pf1N1B4_1190 - Predicted D-lactate dehydrogenase, Fe-S protein, FAD/FMN-containing, at 1,341,300 to 1,344,110
_1190
Position (kb)
1339
1340
1341 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1338.203 kb on + strand at 1338.203 kb on + strand at 1338.203 kb on + strand at 1338.204 kb on - strand at 1338.204 kb on - strand at 1338.204 kb on - strand at 1338.211 kb on - strand at 1338.260 kb on + strand at 1338.260 kb on + strand at 1338.261 kb on - strand at 1338.303 kb on + strand at 1338.303 kb on + strand at 1338.303 kb on + strand at 1338.303 kb on + strand at 1338.303 kb on + strand at 1338.303 kb on + strand at 1338.303 kb on + strand at 1338.304 kb on - strand at 1338.304 kb on - strand at 1338.304 kb on - strand at 1338.304 kb on - strand at 1338.304 kb on - strand at 1338.304 kb on - strand at 1338.304 kb on - strand at 1338.305 kb on + strand at 1338.306 kb on - strand at 1338.306 kb on - strand at 1338.306 kb on - strand at 1338.306 kb on - strand at 1338.306 kb on - strand at 1338.373 kb on - strand at 1338.373 kb on - strand at 1338.429 kb on + strand at 1338.429 kb on + strand at 1338.456 kb on + strand, within Pf1N1B4_1187 at 1338.457 kb on - strand, within Pf1N1B4_1187 at 1338.585 kb on + strand, within Pf1N1B4_1187 at 1338.585 kb on + strand, within Pf1N1B4_1187 at 1338.586 kb on - strand, within Pf1N1B4_1187 at 1338.705 kb on + strand, within Pf1N1B4_1187 at 1338.705 kb on + strand, within Pf1N1B4_1187 at 1338.705 kb on + strand, within Pf1N1B4_1187 at 1338.706 kb on - strand, within Pf1N1B4_1187 at 1338.771 kb on + strand, within Pf1N1B4_1187 at 1338.771 kb on + strand, within Pf1N1B4_1187 at 1338.771 kb on + strand at 1338.771 kb on + strand, within Pf1N1B4_1187 at 1338.771 kb on + strand, within Pf1N1B4_1187 at 1338.772 kb on - strand, within Pf1N1B4_1187 at 1338.772 kb on - strand, within Pf1N1B4_1187 at 1338.772 kb on - strand, within Pf1N1B4_1187 at 1338.772 kb on - strand, within Pf1N1B4_1187 at 1338.772 kb on - strand, within Pf1N1B4_1187 at 1338.772 kb on - strand, within Pf1N1B4_1187 at 1338.772 kb on - strand, within Pf1N1B4_1187 at 1338.916 kb on + strand, within Pf1N1B4_1187 at 1338.916 kb on + strand, within Pf1N1B4_1187 at 1338.916 kb on + strand, within Pf1N1B4_1187 at 1338.916 kb on + strand, within Pf1N1B4_1187 at 1338.917 kb on - strand, within Pf1N1B4_1187 at 1338.941 kb on + strand, within Pf1N1B4_1187 at 1338.941 kb on + strand, within Pf1N1B4_1187 at 1338.942 kb on - strand, within Pf1N1B4_1187 at 1338.990 kb on - strand, within Pf1N1B4_1187 at 1338.990 kb on - strand, within Pf1N1B4_1187 at 1338.998 kb on + strand, within Pf1N1B4_1187 at 1338.998 kb on + strand, within Pf1N1B4_1187 at 1338.998 kb on + strand, within Pf1N1B4_1187 at 1338.998 kb on + strand, within Pf1N1B4_1187 at 1338.999 kb on - strand, within Pf1N1B4_1187 at 1338.999 kb on - strand, within Pf1N1B4_1187 at 1338.999 kb on - strand, within Pf1N1B4_1187 at 1339.195 kb on + strand at 1339.195 kb on + strand at 1339.196 kb on + strand at 1339.201 kb on + strand at 1339.202 kb on - strand at 1339.212 kb on + strand at 1339.212 kb on + strand at 1339.212 kb on + strand at 1339.213 kb on - strand at 1339.213 kb on - strand at 1339.213 kb on - strand at 1339.383 kb on + strand, within Pf1N1B4_1188 at 1339.488 kb on + strand, within Pf1N1B4_1188 at 1339.488 kb on + strand, within Pf1N1B4_1188 at 1339.489 kb on - strand, within Pf1N1B4_1188 at 1339.657 kb on + strand, within Pf1N1B4_1188 at 1339.657 kb on + strand, within Pf1N1B4_1188 at 1339.658 kb on - strand, within Pf1N1B4_1188 at 1339.658 kb on - strand, within Pf1N1B4_1188 at 1339.724 kb on + strand, within Pf1N1B4_1188 at 1339.724 kb on + strand, within Pf1N1B4_1188 at 1339.724 kb on + strand, within Pf1N1B4_1188 at 1339.725 kb on - strand, within Pf1N1B4_1188 at 1339.725 kb on - strand, within Pf1N1B4_1188 at 1339.737 kb on + strand, within Pf1N1B4_1188 at 1339.738 kb on - strand, within Pf1N1B4_1188 at 1339.738 kb on - strand, within Pf1N1B4_1188 at 1339.738 kb on - strand, within Pf1N1B4_1188 at 1339.738 kb on - strand, within Pf1N1B4_1188 at 1339.896 kb on + strand, within Pf1N1B4_1188 at 1339.896 kb on + strand, within Pf1N1B4_1188 at 1339.896 kb on + strand, within Pf1N1B4_1188 at 1339.897 kb on - strand, within Pf1N1B4_1188 at 1339.897 kb on - strand, within Pf1N1B4_1188 at 1339.897 kb on - strand, within Pf1N1B4_1188 at 1339.900 kb on + strand, within Pf1N1B4_1188 at 1339.900 kb on + strand, within Pf1N1B4_1188 at 1339.900 kb on + strand, within Pf1N1B4_1188 at 1339.900 kb on + strand, within Pf1N1B4_1188 at 1339.900 kb on + strand, within Pf1N1B4_1188 at 1339.900 kb on + strand, within Pf1N1B4_1188 at 1339.900 kb on + strand, within Pf1N1B4_1188 at 1339.900 kb on + strand, within Pf1N1B4_1188 at 1339.900 kb on + strand, within Pf1N1B4_1188 at 1339.900 kb on + strand at 1339.901 kb on - strand, within Pf1N1B4_1188 at 1339.901 kb on - strand, within Pf1N1B4_1188 at 1339.901 kb on - strand, within Pf1N1B4_1188 at 1339.901 kb on - strand, within Pf1N1B4_1188 at 1339.901 kb on - strand, within Pf1N1B4_1188 at 1339.901 kb on - strand, within Pf1N1B4_1188 at 1339.901 kb on - strand, within Pf1N1B4_1188 at 1339.901 kb on - strand, within Pf1N1B4_1188 at 1339.901 kb on - strand, within Pf1N1B4_1188 at 1339.901 kb on - strand, within Pf1N1B4_1188 at 1339.950 kb on + strand, within Pf1N1B4_1188 at 1339.950 kb on + strand, within Pf1N1B4_1188 at 1340.003 kb on + strand, within Pf1N1B4_1188 at 1340.003 kb on + strand, within Pf1N1B4_1188 at 1340.003 kb on + strand, within Pf1N1B4_1188 at 1340.003 kb on + strand, within Pf1N1B4_1188 at 1340.003 kb on + strand, within Pf1N1B4_1188 at 1340.004 kb on - strand, within Pf1N1B4_1188 at 1340.004 kb on - strand, within Pf1N1B4_1188 at 1340.004 kb on - strand, within Pf1N1B4_1188 at 1340.004 kb on - strand, within Pf1N1B4_1188 at 1340.004 kb on - strand, within Pf1N1B4_1188 at 1340.152 kb on + strand, within Pf1N1B4_1188 at 1340.153 kb on - strand, within Pf1N1B4_1188 at 1340.153 kb on - strand, within Pf1N1B4_1188 at 1340.153 kb on - strand, within Pf1N1B4_1188 at 1340.153 kb on - strand, within Pf1N1B4_1188 at 1340.153 kb on - strand, within Pf1N1B4_1188 at 1340.153 kb on - strand, within Pf1N1B4_1188 at 1340.171 kb on + strand, within Pf1N1B4_1188 at 1340.172 kb on - strand, within Pf1N1B4_1188 at 1340.196 kb on - strand, within Pf1N1B4_1188 at 1340.196 kb on - strand, within Pf1N1B4_1188 at 1340.417 kb on + strand, within Pf1N1B4_1188 at 1340.417 kb on + strand, within Pf1N1B4_1188 at 1340.417 kb on + strand, within Pf1N1B4_1188 at 1340.418 kb on - strand, within Pf1N1B4_1188 at 1340.418 kb on - strand, within Pf1N1B4_1188 at 1340.438 kb on - strand, within Pf1N1B4_1188 at 1340.649 kb on + strand at 1340.649 kb on + strand at 1340.650 kb on - strand at 1340.650 kb on - strand at 1340.650 kb on - strand at 1340.731 kb on + strand, within Pf1N1B4_1189 at 1340.737 kb on + strand, within Pf1N1B4_1189 at 1340.737 kb on + strand, within Pf1N1B4_1189 at 1340.737 kb on + strand, within Pf1N1B4_1189 at 1340.737 kb on + strand, within Pf1N1B4_1189 at 1340.737 kb on + strand, within Pf1N1B4_1189 at 1340.738 kb on - strand, within Pf1N1B4_1189 at 1340.738 kb on - strand, within Pf1N1B4_1189 at 1340.738 kb on - strand, within Pf1N1B4_1189 at 1340.738 kb on - strand, within Pf1N1B4_1189 at 1340.738 kb on - strand, within Pf1N1B4_1189 at 1340.738 kb on - strand, within Pf1N1B4_1189 at 1340.738 kb on - strand, within Pf1N1B4_1189 at 1340.953 kb on + strand, within Pf1N1B4_1189 at 1340.953 kb on + strand at 1340.953 kb on + strand, within Pf1N1B4_1189 at 1340.954 kb on - strand, within Pf1N1B4_1189 at 1341.046 kb on - strand, within Pf1N1B4_1189 at 1341.242 kb on + strand at 1341.242 kb on + strand at 1341.242 kb on + strand at 1341.242 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction D,L-Lactate (C) remove 1,338,203 + -1.9 1,338,203 + -1.5 1,338,203 + -2.6 1,338,204 - -1.3 1,338,204 - -2.4 1,338,204 - +0.4 1,338,211 - -2.3 1,338,260 + -3.1 1,338,260 + +1.0 1,338,261 - -1.9 1,338,303 + -1.0 1,338,303 + +0.9 1,338,303 + -0.6 1,338,303 + -3.8 1,338,303 + -1.7 1,338,303 + -3.1 1,338,303 + -1.3 1,338,304 - -1.7 1,338,304 - +0.6 1,338,304 - +0.5 1,338,304 - -1.3 1,338,304 - -1.3 1,338,304 - +0.2 1,338,304 - -0.3 1,338,305 + -2.7 1,338,306 - -1.0 1,338,306 - -0.3 1,338,306 - -3.1 1,338,306 - -2.5 1,338,306 - -2.8 1,338,373 - -2.8 1,338,373 - -1.9 1,338,429 + -0.3 1,338,429 + -1.7 1,338,456 + Pf1N1B4_1187 0.12 -2.5 1,338,457 - Pf1N1B4_1187 0.12 -0.7 1,338,585 + Pf1N1B4_1187 0.28 -2.6 1,338,585 + Pf1N1B4_1187 0.28 -1.7 1,338,586 - Pf1N1B4_1187 0.28 -0.7 1,338,705 + Pf1N1B4_1187 0.42 -2.2 1,338,705 + Pf1N1B4_1187 0.42 +0.2 1,338,705 + Pf1N1B4_1187 0.42 -0.5 1,338,706 - Pf1N1B4_1187 0.42 +0.5 1,338,771 + Pf1N1B4_1187 0.50 -2.8 1,338,771 + Pf1N1B4_1187 0.50 -1.5 1,338,771 + -0.3 1,338,771 + Pf1N1B4_1187 0.50 -0.7 1,338,771 + Pf1N1B4_1187 0.50 -0.7 1,338,772 - Pf1N1B4_1187 0.50 -1.0 1,338,772 - Pf1N1B4_1187 0.50 -0.3 1,338,772 - Pf1N1B4_1187 0.50 -0.7 1,338,772 - Pf1N1B4_1187 0.50 -2.4 1,338,772 - Pf1N1B4_1187 0.50 -1.3 1,338,772 - Pf1N1B4_1187 0.50 -0.7 1,338,772 - Pf1N1B4_1187 0.50 -0.7 1,338,916 + Pf1N1B4_1187 0.68 -2.2 1,338,916 + Pf1N1B4_1187 0.68 -3.0 1,338,916 + Pf1N1B4_1187 0.68 -1.3 1,338,916 + Pf1N1B4_1187 0.68 -1.0 1,338,917 - Pf1N1B4_1187 0.68 -0.7 1,338,941 + Pf1N1B4_1187 0.71 -2.9 1,338,941 + Pf1N1B4_1187 0.71 -2.0 1,338,942 - Pf1N1B4_1187 0.71 -1.1 1,338,990 - Pf1N1B4_1187 0.77 -3.4 1,338,990 - Pf1N1B4_1187 0.77 +1.3 1,338,998 + Pf1N1B4_1187 0.78 -1.5 1,338,998 + Pf1N1B4_1187 0.78 -0.3 1,338,998 + Pf1N1B4_1187 0.78 -1.9 1,338,998 + Pf1N1B4_1187 0.78 -1.9 1,338,999 - Pf1N1B4_1187 0.78 +1.3 1,338,999 - Pf1N1B4_1187 0.78 -1.3 1,338,999 - Pf1N1B4_1187 0.78 -3.0 1,339,195 + -2.0 1,339,195 + -1.6 1,339,196 + -2.9 1,339,201 + -3.2 1,339,202 - -2.0 1,339,212 + -1.7 1,339,212 + -0.7 1,339,212 + -1.7 1,339,213 - -1.9 1,339,213 - -1.7 1,339,213 - -3.1 1,339,383 + Pf1N1B4_1188 0.14 -0.9 1,339,488 + Pf1N1B4_1188 0.21 -3.0 1,339,488 + Pf1N1B4_1188 0.21 -2.0 1,339,489 - Pf1N1B4_1188 0.21 -1.0 1,339,657 + Pf1N1B4_1188 0.33 -1.5 1,339,657 + Pf1N1B4_1188 0.33 -1.0 1,339,658 - Pf1N1B4_1188 0.33 -0.9 1,339,658 - Pf1N1B4_1188 0.33 -2.2 1,339,724 + Pf1N1B4_1188 0.38 -1.9 1,339,724 + Pf1N1B4_1188 0.38 -1.7 1,339,724 + Pf1N1B4_1188 0.38 -2.0 1,339,725 - Pf1N1B4_1188 0.38 -2.7 1,339,725 - Pf1N1B4_1188 0.38 -1.7 1,339,737 + Pf1N1B4_1188 0.38 -3.7 1,339,738 - Pf1N1B4_1188 0.38 -0.3 1,339,738 - Pf1N1B4_1188 0.38 -3.3 1,339,738 - Pf1N1B4_1188 0.38 -1.9 1,339,738 - Pf1N1B4_1188 0.38 -2.4 1,339,896 + Pf1N1B4_1188 0.49 -0.7 1,339,896 + Pf1N1B4_1188 0.49 -1.0 1,339,896 + Pf1N1B4_1188 0.49 -1.0 1,339,897 - Pf1N1B4_1188 0.49 -1.0 1,339,897 - Pf1N1B4_1188 0.49 -1.9 1,339,897 - Pf1N1B4_1188 0.49 -2.0 1,339,900 + Pf1N1B4_1188 0.50 -1.0 1,339,900 + Pf1N1B4_1188 0.50 -1.3 1,339,900 + Pf1N1B4_1188 0.50 -1.3 1,339,900 + Pf1N1B4_1188 0.50 -1.0 1,339,900 + Pf1N1B4_1188 0.50 -3.0 1,339,900 + Pf1N1B4_1188 0.50 -1.5 1,339,900 + Pf1N1B4_1188 0.50 -0.3 1,339,900 + Pf1N1B4_1188 0.50 -2.5 1,339,900 + Pf1N1B4_1188 0.50 -3.9 1,339,900 + +1.3 1,339,901 - Pf1N1B4_1188 0.50 -1.5 1,339,901 - Pf1N1B4_1188 0.50 -0.7 1,339,901 - Pf1N1B4_1188 0.50 -2.4 1,339,901 - Pf1N1B4_1188 0.50 -2.4 1,339,901 - Pf1N1B4_1188 0.50 -1.4 1,339,901 - Pf1N1B4_1188 0.50 -2.8 1,339,901 - Pf1N1B4_1188 0.50 -2.7 1,339,901 - Pf1N1B4_1188 0.50 -1.0 1,339,901 - Pf1N1B4_1188 0.50 -2.0 1,339,901 - Pf1N1B4_1188 0.50 -1.3 1,339,950 + Pf1N1B4_1188 0.53 -2.4 1,339,950 + Pf1N1B4_1188 0.53 -1.7 1,340,003 + Pf1N1B4_1188 0.57 -1.9 1,340,003 + Pf1N1B4_1188 0.57 -3.0 1,340,003 + Pf1N1B4_1188 0.57 -1.9 1,340,003 + Pf1N1B4_1188 0.57 -0.0 1,340,003 + Pf1N1B4_1188 0.57 -1.6 1,340,004 - Pf1N1B4_1188 0.57 -0.7 1,340,004 - Pf1N1B4_1188 0.57 +0.3 1,340,004 - Pf1N1B4_1188 0.57 -0.3 1,340,004 - Pf1N1B4_1188 0.57 -1.7 1,340,004 - Pf1N1B4_1188 0.57 -1.5 1,340,152 + Pf1N1B4_1188 0.67 -1.5 1,340,153 - Pf1N1B4_1188 0.67 -2.1 1,340,153 - Pf1N1B4_1188 0.67 -3.0 1,340,153 - Pf1N1B4_1188 0.67 -1.0 1,340,153 - Pf1N1B4_1188 0.67 -0.8 1,340,153 - Pf1N1B4_1188 0.67 -2.0 1,340,153 - Pf1N1B4_1188 0.67 -1.0 1,340,171 + Pf1N1B4_1188 0.68 -2.2 1,340,172 - Pf1N1B4_1188 0.68 +0.3 1,340,196 - Pf1N1B4_1188 0.70 -0.9 1,340,196 - Pf1N1B4_1188 0.70 -1.2 1,340,417 + Pf1N1B4_1188 0.85 +0.4 1,340,417 + Pf1N1B4_1188 0.85 -1.9 1,340,417 + Pf1N1B4_1188 0.85 -1.4 1,340,418 - Pf1N1B4_1188 0.85 -1.7 1,340,418 - Pf1N1B4_1188 0.85 -0.8 1,340,438 - Pf1N1B4_1188 0.87 -1.0 1,340,649 + -1.7 1,340,649 + -1.7 1,340,650 - -1.7 1,340,650 - -1.5 1,340,650 - -3.6 1,340,731 + Pf1N1B4_1189 0.15 -1.7 1,340,737 + Pf1N1B4_1189 0.16 -3.7 1,340,737 + Pf1N1B4_1189 0.16 -2.6 1,340,737 + Pf1N1B4_1189 0.16 -1.3 1,340,737 + Pf1N1B4_1189 0.16 -1.9 1,340,737 + Pf1N1B4_1189 0.16 -2.4 1,340,738 - Pf1N1B4_1189 0.16 -0.3 1,340,738 - Pf1N1B4_1189 0.16 -1.7 1,340,738 - Pf1N1B4_1189 0.16 -1.0 1,340,738 - Pf1N1B4_1189 0.16 +0.3 1,340,738 - Pf1N1B4_1189 0.16 -2.5 1,340,738 - Pf1N1B4_1189 0.16 -1.4 1,340,738 - Pf1N1B4_1189 0.16 -1.3 1,340,953 + Pf1N1B4_1189 0.48 -1.7 1,340,953 + -0.3 1,340,953 + Pf1N1B4_1189 0.48 -0.3 1,340,954 - Pf1N1B4_1189 0.48 -3.6 1,341,046 - Pf1N1B4_1189 0.62 -3.7 1,341,242 + -1.3 1,341,242 + -1.3 1,341,242 + -2.3 1,341,242 + -1.7
Or see this region's nucleotide sequence