Experiment: Growth at 30C; with MOPS
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PFLU_RS00865 and PFLU_RS00870 are separated by 369 nucleotides PFLU_RS00870 and PFLU_RS00875 are separated by 93 nucleotides PFLU_RS00875 and PFLU_RS00880 are separated by 98 nucleotides
PFLU_RS00865: PFLU_RS00865 - hypothetical protein, at 193,419 to 195,041
_RS00865
PFLU_RS00870: PFLU_RS00870 - hypothetical protein, at 195,411 to 195,590
_RS00870
PFLU_RS00875: PFLU_RS00875 - hypothetical protein, at 195,684 to 196,526
_RS00875
PFLU_RS00880: PFLU_RS00880 - DUF927 domain-containing protein, at 196,625 to 199,513
_RS00880
Position (kb) 195
196
197 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 194.693 kb on + strand, within PFLU_RS00865 at 194.793 kb on + strand, within PFLU_RS00865 at 194.794 kb on - strand, within PFLU_RS00865 at 194.828 kb on + strand, within PFLU_RS00865 at 194.835 kb on - strand, within PFLU_RS00865 at 194.952 kb on + strand at 195.079 kb on - strand at 195.155 kb on - strand at 195.167 kb on + strand at 195.167 kb on + strand at 195.168 kb on - strand at 195.204 kb on - strand at 195.264 kb on - strand at 195.266 kb on - strand at 195.359 kb on + strand at 195.359 kb on + strand at 195.374 kb on + strand at 195.393 kb on + strand at 195.498 kb on + strand, within PFLU_RS00870 at 195.546 kb on + strand, within PFLU_RS00870 at 195.547 kb on - strand, within PFLU_RS00870 at 195.576 kb on + strand at 195.577 kb on - strand at 195.577 kb on - strand at 195.577 kb on - strand at 195.580 kb on + strand at 195.581 kb on - strand at 195.581 kb on - strand at 195.614 kb on + strand at 195.614 kb on + strand at 195.614 kb on + strand at 195.625 kb on - strand at 195.625 kb on - strand at 195.625 kb on - strand at 195.696 kb on + strand at 195.705 kb on + strand at 195.705 kb on + strand at 195.705 kb on + strand at 195.705 kb on + strand at 195.705 kb on + strand at 195.705 kb on + strand at 195.705 kb on + strand at 195.705 kb on + strand at 195.705 kb on + strand at 195.705 kb on + strand at 195.706 kb on - strand at 195.706 kb on - strand at 195.706 kb on - strand at 195.706 kb on - strand at 195.706 kb on - strand at 195.706 kb on - strand at 195.888 kb on + strand, within PFLU_RS00875 at 195.888 kb on + strand, within PFLU_RS00875 at 195.889 kb on - strand, within PFLU_RS00875 at 195.954 kb on + strand, within PFLU_RS00875 at 195.954 kb on + strand, within PFLU_RS00875 at 195.954 kb on + strand, within PFLU_RS00875 at 195.954 kb on + strand, within PFLU_RS00875 at 195.954 kb on + strand, within PFLU_RS00875 at 195.954 kb on + strand, within PFLU_RS00875 at 195.954 kb on + strand, within PFLU_RS00875 at 195.954 kb on + strand, within PFLU_RS00875 at 195.955 kb on - strand, within PFLU_RS00875 at 195.955 kb on - strand, within PFLU_RS00875 at 196.070 kb on + strand, within PFLU_RS00875 at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.464 kb on + strand at 196.465 kb on - strand at 196.465 kb on - strand at 196.465 kb on - strand at 196.465 kb on - strand at 196.465 kb on - strand at 196.465 kb on - strand at 196.465 kb on - strand at 196.465 kb on - strand at 196.465 kb on - strand at 196.465 kb on - strand at 196.465 kb on - strand at 196.465 kb on - strand at 196.465 kb on - strand at 196.465 kb on - strand at 196.473 kb on + strand at 196.474 kb on - strand at 196.530 kb on + strand at 196.530 kb on + strand at 196.530 kb on + strand at 196.530 kb on + strand at 196.530 kb on + strand at 196.530 kb on + strand at 196.530 kb on + strand at 196.530 kb on + strand at 196.530 kb on + strand at 196.530 kb on + strand at 196.530 kb on + strand at 196.531 kb on - strand at 196.531 kb on - strand at 196.543 kb on + strand at 196.544 kb on - strand at 196.544 kb on - strand at 196.544 kb on - strand at 196.544 kb on - strand at 196.544 kb on - strand at 196.591 kb on + strand at 196.591 kb on + strand at 196.592 kb on - strand at 196.592 kb on - strand at 196.603 kb on - strand at 196.677 kb on + strand at 196.677 kb on + strand at 196.677 kb on + strand at 196.677 kb on + strand at 196.677 kb on + strand at 196.677 kb on + strand at 196.678 kb on - strand at 196.678 kb on - strand at 196.678 kb on - strand at 196.678 kb on - strand at 196.829 kb on + strand at 196.830 kb on - strand at 196.830 kb on - strand at 196.830 kb on - strand at 196.830 kb on - strand at 196.830 kb on - strand at 196.830 kb on - strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.833 kb on + strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.834 kb on - strand at 196.836 kb on + strand at 196.836 kb on + strand at 196.836 kb on + strand at 196.836 kb on + strand at 196.836 kb on + strand at 196.836 kb on + strand at 196.836 kb on + strand at 196.836 kb on + strand at 196.836 kb on + strand at 196.836 kb on + strand at 196.836 kb on + strand at 196.836 kb on + strand at 196.837 kb on - strand at 196.837 kb on - strand at 196.837 kb on - strand at 196.837 kb on - strand at 196.837 kb on - strand at 196.958 kb on - strand, within PFLU_RS00880 at 196.994 kb on + strand, within PFLU_RS00880 at 196.995 kb on - strand, within PFLU_RS00880 at 197.004 kb on - strand, within PFLU_RS00880 at 197.004 kb on - strand, within PFLU_RS00880 at 197.004 kb on - strand, within PFLU_RS00880 at 197.004 kb on - strand, within PFLU_RS00880 at 197.307 kb on + strand, within PFLU_RS00880 at 197.308 kb on - strand, within PFLU_RS00880 at 197.308 kb on - strand, within PFLU_RS00880 at 197.308 kb on - strand, within PFLU_RS00880 at 197.308 kb on - strand, within PFLU_RS00880 at 197.310 kb on + strand, within PFLU_RS00880 at 197.310 kb on + strand, within PFLU_RS00880 at 197.310 kb on + strand, within PFLU_RS00880 at 197.310 kb on + strand, within PFLU_RS00880 at 197.310 kb on + strand, within PFLU_RS00880 at 197.311 kb on - strand, within PFLU_RS00880 at 197.311 kb on - strand, within PFLU_RS00880 at 197.311 kb on - strand, within PFLU_RS00880 at 197.311 kb on - strand, within PFLU_RS00880 at 197.311 kb on - strand, within PFLU_RS00880 at 197.311 kb on - strand, within PFLU_RS00880 at 197.311 kb on - strand, within PFLU_RS00880 at 197.311 kb on - strand, within PFLU_RS00880 at 197.387 kb on + strand, within PFLU_RS00880 at 197.387 kb on + strand, within PFLU_RS00880 at 197.388 kb on - strand, within PFLU_RS00880 at 197.388 kb on - strand, within PFLU_RS00880
Per-strain Table
Position Strand Gene LocusTag Fraction Growth at 30C; with MOPS remove 194,693 + PFLU_RS00865 0.78 +0.9 194,793 + PFLU_RS00865 0.85 +0.3 194,794 - PFLU_RS00865 0.85 -1.1 194,828 + PFLU_RS00865 0.87 +1.3 194,835 - PFLU_RS00865 0.87 +0.1 194,952 + -1.2 195,079 - -0.2 195,155 - -1.5 195,167 + +0.3 195,167 + -0.8 195,168 - +0.9 195,204 - -0.5 195,264 - -0.0 195,266 - -1.5 195,359 + -0.2 195,359 + -0.7 195,374 + -0.7 195,393 + +1.1 195,498 + PFLU_RS00870 0.48 +0.6 195,546 + PFLU_RS00870 0.75 +0.6 195,547 - PFLU_RS00870 0.76 +0.0 195,576 + +1.9 195,577 - -0.5 195,577 - +0.6 195,577 - +0.4 195,580 + +0.7 195,581 - +1.4 195,581 - +2.5 195,614 + +3.1 195,614 + +0.4 195,614 + -1.1 195,625 - -2.1 195,625 - -1.3 195,625 - -0.1 195,696 + -0.1 195,705 + +0.6 195,705 + +1.1 195,705 + +0.4 195,705 + +1.1 195,705 + -1.5 195,705 + +0.8 195,705 + +0.6 195,705 + -0.2 195,705 + +0.9 195,705 + +1.5 195,706 - -0.3 195,706 - +0.8 195,706 - +0.5 195,706 - -0.9 195,706 - +0.5 195,706 - +0.2 195,888 + PFLU_RS00875 0.24 +1.0 195,888 + PFLU_RS00875 0.24 +0.1 195,889 - PFLU_RS00875 0.24 -0.7 195,954 + PFLU_RS00875 0.32 -0.3 195,954 + PFLU_RS00875 0.32 -0.7 195,954 + PFLU_RS00875 0.32 -0.1 195,954 + PFLU_RS00875 0.32 +0.6 195,954 + PFLU_RS00875 0.32 -0.2 195,954 + PFLU_RS00875 0.32 +0.8 195,954 + PFLU_RS00875 0.32 -0.6 195,954 + PFLU_RS00875 0.32 -0.3 195,955 - PFLU_RS00875 0.32 +2.2 195,955 - PFLU_RS00875 0.32 -0.1 196,070 + PFLU_RS00875 0.46 -0.3 196,464 + +0.3 196,464 + +1.6 196,464 + +0.9 196,464 + +0.5 196,464 + +1.0 196,464 + +1.7 196,464 + -0.6 196,464 + -0.9 196,464 + -0.6 196,464 + +2.0 196,464 + +0.9 196,464 + -1.3 196,464 + -0.5 196,464 + -1.7 196,464 + -0.6 196,464 + -1.9 196,464 + -0.0 196,464 + +1.1 196,465 - -1.3 196,465 - +1.0 196,465 - -1.8 196,465 - +0.8 196,465 - -1.0 196,465 - +0.1 196,465 - -0.5 196,465 - +0.7 196,465 - -1.3 196,465 - +0.0 196,465 - +0.8 196,465 - +0.9 196,465 - -0.6 196,465 - +1.3 196,473 + -1.1 196,474 - +0.5 196,530 + +0.1 196,530 + +0.7 196,530 + -1.3 196,530 + -0.7 196,530 + +0.5 196,530 + -0.2 196,530 + +0.1 196,530 + -0.9 196,530 + -1.7 196,530 + -0.3 196,530 + +0.3 196,531 - -0.9 196,531 - -0.2 196,543 + +1.1 196,544 - +2.0 196,544 - +0.7 196,544 - -0.7 196,544 - +1.5 196,544 - +0.3 196,591 + -0.6 196,591 + +0.7 196,592 - -0.6 196,592 - +1.3 196,603 - +1.7 196,677 + -0.4 196,677 + +1.3 196,677 + +0.3 196,677 + +1.0 196,677 + -0.5 196,677 + -1.1 196,678 - +1.1 196,678 - +1.3 196,678 - -0.7 196,678 - +1.3 196,829 + +0.5 196,830 - +1.2 196,830 - +0.7 196,830 - +1.3 196,830 - -0.7 196,830 - -0.1 196,830 - +1.9 196,833 + -0.5 196,833 + +0.0 196,833 + +2.1 196,833 + -0.0 196,833 + +0.7 196,833 + +1.9 196,833 + +0.6 196,833 + +0.4 196,833 + -0.0 196,833 + -0.0 196,833 + +0.2 196,833 + +0.8 196,833 + -1.1 196,833 + -1.0 196,833 + -0.7 196,833 + -0.7 196,833 + +1.0 196,833 + +0.6 196,833 + +0.6 196,833 + -1.2 196,833 + +2.1 196,833 + +0.7 196,834 - +1.9 196,834 - +0.5 196,834 - +1.2 196,834 - +1.5 196,834 - +0.7 196,834 - -0.5 196,834 - -0.7 196,834 - +2.9 196,834 - +0.3 196,834 - -0.3 196,834 - +1.1 196,834 - +0.9 196,834 - +4.2 196,834 - +2.3 196,834 - +0.5 196,834 - +1.3 196,834 - +0.9 196,834 - +0.3 196,834 - -0.6 196,834 - -0.1 196,834 - -0.3 196,834 - +0.5 196,834 - -0.5 196,834 - -1.1 196,836 + -0.5 196,836 + -0.6 196,836 + +0.2 196,836 + +0.6 196,836 + -1.0 196,836 + +0.8 196,836 + +2.0 196,836 + -1.8 196,836 + -0.3 196,836 + +1.3 196,836 + -1.1 196,836 + -0.1 196,837 - -0.1 196,837 - +1.9 196,837 - -0.7 196,837 - +1.1 196,837 - +1.9 196,958 - PFLU_RS00880 0.12 -0.1 196,994 + PFLU_RS00880 0.13 +0.4 196,995 - PFLU_RS00880 0.13 +0.9 197,004 - PFLU_RS00880 0.13 -0.1 197,004 - PFLU_RS00880 0.13 +1.1 197,004 - PFLU_RS00880 0.13 +0.3 197,004 - PFLU_RS00880 0.13 +2.5 197,307 + PFLU_RS00880 0.24 +0.9 197,308 - PFLU_RS00880 0.24 -1.0 197,308 - PFLU_RS00880 0.24 +2.4 197,308 - PFLU_RS00880 0.24 +0.8 197,308 - PFLU_RS00880 0.24 +1.4 197,310 + PFLU_RS00880 0.24 -0.6 197,310 + PFLU_RS00880 0.24 +1.0 197,310 + PFLU_RS00880 0.24 -0.1 197,310 + PFLU_RS00880 0.24 -0.3 197,310 + PFLU_RS00880 0.24 -0.5 197,311 - PFLU_RS00880 0.24 +2.1 197,311 - PFLU_RS00880 0.24 +1.5 197,311 - PFLU_RS00880 0.24 +2.3 197,311 - PFLU_RS00880 0.24 +0.1 197,311 - PFLU_RS00880 0.24 +1.1 197,311 - PFLU_RS00880 0.24 -0.1 197,311 - PFLU_RS00880 0.24 +0.2 197,311 - PFLU_RS00880 0.24 +1.5 197,387 + PFLU_RS00880 0.26 -0.5 197,387 + PFLU_RS00880 0.26 +0.7 197,388 - PFLU_RS00880 0.26 +0.6 197,388 - PFLU_RS00880 0.26 -0.4
Or see this region's nucleotide sequence