Experiment: Dextran 5 mg/ml (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS08365 and HMPREF1058_RS08360 are separated by 52 nucleotides HMPREF1058_RS08360 and HMPREF1058_RS08355 overlap by 11 nucleotides HMPREF1058_RS08355 and HMPREF1058_RS08350 are separated by 171 nucleotides HMPREF1058_RS08350 and HMPREF1058_RS08345 overlap by 4 nucleotides
HMPREF1058_RS08365: HMPREF1058_RS08365 - glycoside hydrolase family 127 protein, at 1,181,189 to 1,183,540
_RS08365
HMPREF1058_RS08360: HMPREF1058_RS08360 - type II toxin-antitoxin system RelE/ParE family toxin, at 1,183,593 to 1,183,934
_RS08360
HMPREF1058_RS08355: HMPREF1058_RS08355 - hypothetical protein, at 1,183,924 to 1,184,199
_RS08355
HMPREF1058_RS08350: HMPREF1058_RS08350 - exonuclease SbcCD subunit D C-terminal domain-containing protein, at 1,184,371 to 1,185,573
_RS08350
HMPREF1058_RS08345: HMPREF1058_RS08345 - AAA family ATPase, at 1,185,570 to 1,188,653
_RS08345
Position (kb)
1184
1185
1186 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1183.438 kb on - strand at 1183.447 kb on + strand at 1183.447 kb on + strand at 1183.448 kb on - strand at 1183.448 kb on - strand at 1183.510 kb on + strand at 1183.510 kb on + strand at 1183.545 kb on - strand at 1183.545 kb on - strand at 1183.551 kb on - strand at 1183.662 kb on - strand, within HMPREF1058_RS08360 at 1183.765 kb on - strand, within HMPREF1058_RS08360 at 1183.776 kb on + strand, within HMPREF1058_RS08360 at 1183.777 kb on - strand, within HMPREF1058_RS08360 at 1183.869 kb on - strand, within HMPREF1058_RS08360 at 1183.869 kb on - strand, within HMPREF1058_RS08360 at 1183.890 kb on - strand, within HMPREF1058_RS08360 at 1184.039 kb on + strand, within HMPREF1058_RS08355 at 1184.222 kb on - strand at 1184.226 kb on - strand at 1184.306 kb on - strand at 1184.495 kb on + strand, within HMPREF1058_RS08350 at 1184.495 kb on + strand, within HMPREF1058_RS08350 at 1184.496 kb on - strand, within HMPREF1058_RS08350 at 1184.557 kb on + strand, within HMPREF1058_RS08350 at 1184.608 kb on - strand, within HMPREF1058_RS08350 at 1184.674 kb on - strand, within HMPREF1058_RS08350 at 1184.721 kb on + strand, within HMPREF1058_RS08350 at 1184.721 kb on + strand, within HMPREF1058_RS08350 at 1184.723 kb on + strand, within HMPREF1058_RS08350 at 1184.724 kb on - strand, within HMPREF1058_RS08350 at 1185.337 kb on + strand, within HMPREF1058_RS08350 at 1185.338 kb on - strand, within HMPREF1058_RS08350 at 1185.343 kb on + strand, within HMPREF1058_RS08350 at 1185.503 kb on - strand at 1185.552 kb on - strand at 1185.577 kb on + strand at 1185.623 kb on + strand at 1185.664 kb on + strand at 1185.665 kb on - strand at 1185.674 kb on + strand at 1185.740 kb on + strand at 1185.749 kb on + strand at 1185.750 kb on - strand at 1186.016 kb on - strand, within HMPREF1058_RS08345 at 1186.184 kb on + strand, within HMPREF1058_RS08345 at 1186.184 kb on + strand, within HMPREF1058_RS08345 at 1186.188 kb on + strand, within HMPREF1058_RS08345 at 1186.221 kb on - strand, within HMPREF1058_RS08345 at 1186.506 kb on + strand, within HMPREF1058_RS08345 at 1186.506 kb on + strand, within HMPREF1058_RS08345 at 1186.507 kb on - strand, within HMPREF1058_RS08345 at 1186.509 kb on - strand, within HMPREF1058_RS08345 at 1186.537 kb on + strand, within HMPREF1058_RS08345 at 1186.567 kb on + strand, within HMPREF1058_RS08345
Per-strain Table
Position Strand Gene LocusTag Fraction Dextran 5 mg/ml (C) remove 1,183,438 - -0.5 1,183,447 + -1.4 1,183,447 + +1.4 1,183,448 - +1.0 1,183,448 - -0.3 1,183,510 + -0.5 1,183,510 + +0.6 1,183,545 - -1.4 1,183,545 - +0.5 1,183,551 - +0.4 1,183,662 - HMPREF1058_RS08360 0.20 -3.2 1,183,765 - HMPREF1058_RS08360 0.50 -3.4 1,183,776 + HMPREF1058_RS08360 0.54 -1.4 1,183,777 - HMPREF1058_RS08360 0.54 +1.7 1,183,869 - HMPREF1058_RS08360 0.81 +0.4 1,183,869 - HMPREF1058_RS08360 0.81 -0.1 1,183,890 - HMPREF1058_RS08360 0.87 +1.3 1,184,039 + HMPREF1058_RS08355 0.42 -0.5 1,184,222 - -1.6 1,184,226 - +0.7 1,184,306 - -0.4 1,184,495 + HMPREF1058_RS08350 0.10 -0.9 1,184,495 + HMPREF1058_RS08350 0.10 -0.1 1,184,496 - HMPREF1058_RS08350 0.10 -0.5 1,184,557 + HMPREF1058_RS08350 0.15 +0.2 1,184,608 - HMPREF1058_RS08350 0.20 +1.9 1,184,674 - HMPREF1058_RS08350 0.25 +0.5 1,184,721 + HMPREF1058_RS08350 0.29 +0.7 1,184,721 + HMPREF1058_RS08350 0.29 +2.4 1,184,723 + HMPREF1058_RS08350 0.29 +2.2 1,184,724 - HMPREF1058_RS08350 0.29 +1.3 1,185,337 + HMPREF1058_RS08350 0.80 +1.2 1,185,338 - HMPREF1058_RS08350 0.80 -0.1 1,185,343 + HMPREF1058_RS08350 0.81 +0.0 1,185,503 - -0.2 1,185,552 - -1.1 1,185,577 + -0.0 1,185,623 + +1.5 1,185,664 + +0.1 1,185,665 - -1.3 1,185,674 + -0.5 1,185,740 + -1.6 1,185,749 + -3.4 1,185,750 - +0.1 1,186,016 - HMPREF1058_RS08345 0.14 +0.4 1,186,184 + HMPREF1058_RS08345 0.20 -1.3 1,186,184 + HMPREF1058_RS08345 0.20 -2.4 1,186,188 + HMPREF1058_RS08345 0.20 -0.4 1,186,221 - HMPREF1058_RS08345 0.21 -0.4 1,186,506 + HMPREF1058_RS08345 0.30 -1.3 1,186,506 + HMPREF1058_RS08345 0.30 -0.3 1,186,507 - HMPREF1058_RS08345 0.30 +2.4 1,186,509 - HMPREF1058_RS08345 0.30 +0.4 1,186,537 + HMPREF1058_RS08345 0.31 -0.5 1,186,567 + HMPREF1058_RS08345 0.32 -2.1
Or see this region's nucleotide sequence