Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS29520 and RR42_RS29525 are separated by 35 nucleotides RR42_RS29525 and RR42_RS29530 are separated by 73 nucleotides RR42_RS29530 and RR42_RS29535 are separated by 103 nucleotides
RR42_RS29520: RR42_RS29520 - acyl-CoA hydrolase, at 1,979,968 to 1,980,456
_RS29520
RR42_RS29525: RR42_RS29525 - hypothetical protein, at 1,980,492 to 1,980,746
_RS29525
RR42_RS29530: RR42_RS29530 - malate permease, at 1,980,820 to 1,982,175
_RS29530
RR42_RS29535: RR42_RS29535 - isocitrate dehydrogenase, at 1,982,279 to 1,983,319
_RS29535
Position (kb)
1980
1981
1982
1983 Strain fitness (log2 ratio)
-2
-1
0
1 at 1979.912 kb on + strand at 1979.912 kb on + strand at 1979.960 kb on + strand at 1979.960 kb on + strand at 1979.969 kb on + strand at 1979.969 kb on + strand at 1980.083 kb on - strand, within RR42_RS29520 at 1980.091 kb on + strand, within RR42_RS29520 at 1980.091 kb on + strand, within RR42_RS29520 at 1980.224 kb on + strand, within RR42_RS29520 at 1980.225 kb on - strand, within RR42_RS29520 at 1980.311 kb on + strand, within RR42_RS29520 at 1980.312 kb on - strand, within RR42_RS29520 at 1980.341 kb on + strand, within RR42_RS29520 at 1980.473 kb on + strand at 1980.474 kb on - strand at 1980.553 kb on + strand, within RR42_RS29525 at 1980.685 kb on - strand, within RR42_RS29525 at 1980.685 kb on - strand, within RR42_RS29525 at 1980.746 kb on + strand at 1980.747 kb on - strand at 1980.777 kb on + strand at 1980.778 kb on - strand at 1980.778 kb on - strand at 1980.811 kb on - strand at 1980.849 kb on + strand at 1981.173 kb on - strand, within RR42_RS29530 at 1981.302 kb on - strand, within RR42_RS29530 at 1981.308 kb on + strand, within RR42_RS29530 at 1981.308 kb on + strand, within RR42_RS29530 at 1981.351 kb on - strand, within RR42_RS29530 at 1981.616 kb on + strand, within RR42_RS29530 at 1981.626 kb on + strand, within RR42_RS29530 at 1981.626 kb on + strand, within RR42_RS29530 at 1981.627 kb on - strand, within RR42_RS29530 at 1981.627 kb on - strand, within RR42_RS29530 at 1981.627 kb on - strand, within RR42_RS29530 at 1981.856 kb on + strand, within RR42_RS29530 at 1981.856 kb on + strand, within RR42_RS29530 at 1981.857 kb on - strand, within RR42_RS29530 at 1981.857 kb on - strand, within RR42_RS29530 at 1981.859 kb on + strand, within RR42_RS29530 at 1981.860 kb on - strand, within RR42_RS29530 at 1981.860 kb on - strand, within RR42_RS29530 at 1981.860 kb on - strand, within RR42_RS29530 at 1982.067 kb on + strand at 1982.068 kb on - strand at 1982.152 kb on + strand at 1982.152 kb on + strand at 1982.153 kb on - strand at 1982.153 kb on - strand at 1982.157 kb on + strand at 1982.157 kb on + strand at 1982.157 kb on + strand at 1982.158 kb on - strand at 1982.158 kb on - strand at 1982.158 kb on - strand at 1982.495 kb on - strand, within RR42_RS29535 at 1982.546 kb on - strand, within RR42_RS29535 at 1982.568 kb on - strand, within RR42_RS29535 at 1982.624 kb on - strand, within RR42_RS29535 at 1982.624 kb on - strand, within RR42_RS29535 at 1982.624 kb on - strand, within RR42_RS29535 at 1982.624 kb on - strand, within RR42_RS29535 at 1982.715 kb on + strand, within RR42_RS29535 at 1982.715 kb on + strand, within RR42_RS29535 at 1982.716 kb on - strand, within RR42_RS29535 at 1982.773 kb on + strand, within RR42_RS29535 at 1982.786 kb on + strand, within RR42_RS29535 at 1982.823 kb on + strand, within RR42_RS29535 at 1982.824 kb on - strand, within RR42_RS29535 at 1982.920 kb on - strand, within RR42_RS29535 at 1982.922 kb on + strand, within RR42_RS29535 at 1982.923 kb on - strand, within RR42_RS29535 at 1982.923 kb on - strand, within RR42_RS29535 at 1982.937 kb on + strand, within RR42_RS29535 at 1982.938 kb on - strand, within RR42_RS29535 at 1982.938 kb on - strand, within RR42_RS29535 at 1982.938 kb on - strand, within RR42_RS29535 at 1982.963 kb on + strand, within RR42_RS29535 at 1982.969 kb on + strand, within RR42_RS29535 at 1982.969 kb on + strand, within RR42_RS29535 at 1982.969 kb on + strand, within RR42_RS29535 at 1982.978 kb on - strand, within RR42_RS29535 at 1983.028 kb on - strand, within RR42_RS29535 at 1983.028 kb on - strand, within RR42_RS29535 at 1983.173 kb on - strand, within RR42_RS29535
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 1,979,912 + -0.8 1,979,912 + +0.3 1,979,960 + -0.2 1,979,960 + -0.2 1,979,969 + -1.0 1,979,969 + -0.9 1,980,083 - RR42_RS29520 0.24 -0.9 1,980,091 + RR42_RS29520 0.25 +0.5 1,980,091 + RR42_RS29520 0.25 +0.8 1,980,224 + RR42_RS29520 0.52 -0.9 1,980,225 - RR42_RS29520 0.53 +0.3 1,980,311 + RR42_RS29520 0.70 -0.5 1,980,312 - RR42_RS29520 0.70 +0.6 1,980,341 + RR42_RS29520 0.76 +0.4 1,980,473 + -0.2 1,980,474 - +0.4 1,980,553 + RR42_RS29525 0.24 +0.0 1,980,685 - RR42_RS29525 0.76 -0.6 1,980,685 - RR42_RS29525 0.76 +1.1 1,980,746 + +0.5 1,980,747 - -0.3 1,980,777 + -0.2 1,980,778 - -0.6 1,980,778 - -0.5 1,980,811 - +1.3 1,980,849 + +0.2 1,981,173 - RR42_RS29530 0.26 -1.1 1,981,302 - RR42_RS29530 0.36 -2.2 1,981,308 + RR42_RS29530 0.36 -2.0 1,981,308 + RR42_RS29530 0.36 -0.5 1,981,351 - RR42_RS29530 0.39 -0.1 1,981,616 + RR42_RS29530 0.59 -0.5 1,981,626 + RR42_RS29530 0.59 -2.0 1,981,626 + RR42_RS29530 0.59 -1.3 1,981,627 - RR42_RS29530 0.60 -1.5 1,981,627 - RR42_RS29530 0.60 -1.7 1,981,627 - RR42_RS29530 0.60 -1.1 1,981,856 + RR42_RS29530 0.76 -1.3 1,981,856 + RR42_RS29530 0.76 +0.5 1,981,857 - RR42_RS29530 0.76 -1.3 1,981,857 - RR42_RS29530 0.76 -2.3 1,981,859 + RR42_RS29530 0.77 -1.7 1,981,860 - RR42_RS29530 0.77 -2.0 1,981,860 - RR42_RS29530 0.77 -0.8 1,981,860 - RR42_RS29530 0.77 -0.8 1,982,067 + -0.8 1,982,068 - -1.3 1,982,152 + +0.1 1,982,152 + -1.1 1,982,153 - -1.2 1,982,153 - -1.5 1,982,157 + +0.3 1,982,157 + -0.8 1,982,157 + -0.2 1,982,158 - -1.7 1,982,158 - -2.3 1,982,158 - -0.8 1,982,495 - RR42_RS29535 0.21 -0.5 1,982,546 - RR42_RS29535 0.26 -1.4 1,982,568 - RR42_RS29535 0.28 -0.7 1,982,624 - RR42_RS29535 0.33 -1.0 1,982,624 - RR42_RS29535 0.33 -0.0 1,982,624 - RR42_RS29535 0.33 -0.6 1,982,624 - RR42_RS29535 0.33 -1.4 1,982,715 + RR42_RS29535 0.42 -0.9 1,982,715 + RR42_RS29535 0.42 -0.8 1,982,716 - RR42_RS29535 0.42 -0.2 1,982,773 + RR42_RS29535 0.47 -2.4 1,982,786 + RR42_RS29535 0.49 -1.5 1,982,823 + RR42_RS29535 0.52 -1.8 1,982,824 - RR42_RS29535 0.52 -0.4 1,982,920 - RR42_RS29535 0.62 +0.6 1,982,922 + RR42_RS29535 0.62 -0.2 1,982,923 - RR42_RS29535 0.62 -0.7 1,982,923 - RR42_RS29535 0.62 -1.3 1,982,937 + RR42_RS29535 0.63 +0.8 1,982,938 - RR42_RS29535 0.63 -0.7 1,982,938 - RR42_RS29535 0.63 -1.2 1,982,938 - RR42_RS29535 0.63 -1.2 1,982,963 + RR42_RS29535 0.66 -1.7 1,982,969 + RR42_RS29535 0.66 -2.2 1,982,969 + RR42_RS29535 0.66 -1.0 1,982,969 + RR42_RS29535 0.66 -1.5 1,982,978 - RR42_RS29535 0.67 -0.8 1,983,028 - RR42_RS29535 0.72 +0.5 1,983,028 - RR42_RS29535 0.72 -1.6 1,983,173 - RR42_RS29535 0.86 -1.2
Or see this region's nucleotide sequence