Experiment: Trisodium citrate (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt RR42_RS27055 and RR42_RS27060 overlap by 8 nucleotides RR42_RS27060 and RR42_RS27065 are separated by 393 nucleotides RR42_RS27065 and RR42_RS27070 are separated by 27 nucleotides
RR42_RS27055: RR42_RS27055 - membrane protein, at 1,420,971 to 1,424,111
_RS27055
RR42_RS27060: RR42_RS27060 - cation transporter, at 1,424,104 to 1,425,243
_RS27060
RR42_RS27065: RR42_RS27065 - hypothetical protein, at 1,425,637 to 1,425,993
_RS27065
RR42_RS27070: RR42_RS27070 - membrane protein, at 1,426,021 to 1,426,725
_RS27070
Position (kb) 1424
1425
1426 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1423.439 kb on - strand, within RR42_RS27055 at 1423.439 kb on - strand, within RR42_RS27055 at 1423.694 kb on - strand, within RR42_RS27055 at 1423.700 kb on - strand, within RR42_RS27055 at 1423.977 kb on - strand at 1424.026 kb on + strand at 1424.114 kb on - strand at 1424.114 kb on - strand at 1424.736 kb on - strand, within RR42_RS27060 at 1425.123 kb on + strand, within RR42_RS27060 at 1425.124 kb on - strand, within RR42_RS27060 at 1425.189 kb on + strand at 1425.881 kb on + strand, within RR42_RS27065 at 1425.993 kb on + strand at 1426.150 kb on + strand, within RR42_RS27070 at 1426.150 kb on + strand, within RR42_RS27070 at 1426.151 kb on - strand, within RR42_RS27070 at 1426.151 kb on - strand, within RR42_RS27070 at 1426.151 kb on - strand, within RR42_RS27070
Per-strain Table
Position Strand Gene LocusTag Fraction Trisodium citrate (C) remove 1,423,439 - RR42_RS27055 0.79 -1.9 1,423,439 - RR42_RS27055 0.79 -2.3 1,423,694 - RR42_RS27055 0.87 -1.6 1,423,700 - RR42_RS27055 0.87 +0.1 1,423,977 - -0.1 1,424,026 + -2.2 1,424,114 - +0.3 1,424,114 - +1.0 1,424,736 - RR42_RS27060 0.55 -0.0 1,425,123 + RR42_RS27060 0.89 -1.4 1,425,124 - RR42_RS27060 0.89 -2.8 1,425,189 + -2.4 1,425,881 + RR42_RS27065 0.68 -0.7 1,425,993 + -0.0 1,426,150 + RR42_RS27070 0.18 +0.2 1,426,150 + RR42_RS27070 0.18 -1.2 1,426,151 - RR42_RS27070 0.18 +0.3 1,426,151 - RR42_RS27070 0.18 +0.0 1,426,151 - RR42_RS27070 0.18 +0.1
Or see this region's nucleotide sequence