Experiment: KB with Nitrofurantoin 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3164 and Psyr_RNA47 are separated by 121 nucleotides Psyr_RNA47 and Psyr_3165 are separated by 142 nucleotides Psyr_3165 and Psyr_3166 overlap by 15 nucleotides Psyr_3166 and Psyr_3167 are separated by 16 nucleotides
Psyr_3164: Psyr_3164 - Protein of unknown function UPF0005, at 3,793,117 to 3,793,788
_3164
Psyr_RNA47: Psyr_RNA47 - tRNA-Ser, at 3,793,910 to 3,793,997
_RNA47
Psyr_3165: Psyr_3165 - transcriptional regulator, LysR family, at 3,794,140 to 3,795,048
_3165
Psyr_3166: Psyr_3166 - ABC transporter, periplasmic substrate-binding protein, putative, at 3,795,034 to 3,795,813
_3166
Psyr_3167: Psyr_3167 - Citrate-proton symport, at 3,795,830 to 3,797,131
_3167
Position (kb)
3794
3795
3796 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 3793.170 kb on + strand at 3793.170 kb on + strand at 3793.172 kb on + strand at 3793.172 kb on + strand at 3793.172 kb on + strand at 3793.172 kb on + strand at 3793.172 kb on + strand at 3793.173 kb on - strand at 3793.173 kb on - strand at 3793.173 kb on - strand at 3793.173 kb on - strand at 3793.173 kb on - strand at 3793.180 kb on + strand at 3793.180 kb on + strand at 3793.181 kb on - strand at 3793.181 kb on - strand at 3793.181 kb on - strand at 3793.181 kb on - strand at 3793.181 kb on - strand at 3793.184 kb on + strand at 3793.184 kb on + strand at 3793.184 kb on + strand at 3793.184 kb on + strand at 3793.184 kb on + strand at 3793.184 kb on + strand at 3793.184 kb on + strand at 3793.185 kb on - strand, within Psyr_3164 at 3793.185 kb on - strand, within Psyr_3164 at 3793.185 kb on - strand, within Psyr_3164 at 3793.185 kb on - strand, within Psyr_3164 at 3793.185 kb on - strand, within Psyr_3164 at 3793.185 kb on - strand, within Psyr_3164 at 3793.185 kb on - strand, within Psyr_3164 at 3793.185 kb on - strand, within Psyr_3164 at 3793.208 kb on + strand, within Psyr_3164 at 3793.208 kb on + strand, within Psyr_3164 at 3793.208 kb on + strand, within Psyr_3164 at 3793.208 kb on + strand, within Psyr_3164 at 3793.208 kb on + strand, within Psyr_3164 at 3793.209 kb on - strand, within Psyr_3164 at 3793.209 kb on - strand, within Psyr_3164 at 3793.209 kb on - strand, within Psyr_3164 at 3793.209 kb on - strand, within Psyr_3164 at 3793.209 kb on - strand, within Psyr_3164 at 3793.212 kb on + strand, within Psyr_3164 at 3793.213 kb on - strand, within Psyr_3164 at 3793.213 kb on - strand, within Psyr_3164 at 3793.334 kb on - strand, within Psyr_3164 at 3793.408 kb on + strand, within Psyr_3164 at 3793.408 kb on + strand, within Psyr_3164 at 3793.408 kb on + strand, within Psyr_3164 at 3793.408 kb on + strand, within Psyr_3164 at 3793.408 kb on + strand, within Psyr_3164 at 3793.408 kb on + strand, within Psyr_3164 at 3793.408 kb on + strand, within Psyr_3164 at 3793.408 kb on + strand, within Psyr_3164 at 3793.409 kb on - strand, within Psyr_3164 at 3793.409 kb on - strand, within Psyr_3164 at 3793.409 kb on - strand, within Psyr_3164 at 3793.412 kb on + strand, within Psyr_3164 at 3793.412 kb on + strand, within Psyr_3164 at 3793.412 kb on + strand, within Psyr_3164 at 3793.413 kb on - strand, within Psyr_3164 at 3793.604 kb on + strand, within Psyr_3164 at 3793.604 kb on + strand, within Psyr_3164 at 3793.605 kb on - strand, within Psyr_3164 at 3793.605 kb on - strand, within Psyr_3164 at 3793.709 kb on + strand, within Psyr_3164 at 3793.709 kb on + strand, within Psyr_3164 at 3793.710 kb on - strand, within Psyr_3164 at 3793.713 kb on + strand, within Psyr_3164 at 3793.713 kb on + strand, within Psyr_3164 at 3793.713 kb on + strand, within Psyr_3164 at 3793.713 kb on + strand, within Psyr_3164 at 3793.714 kb on - strand, within Psyr_3164 at 3793.765 kb on - strand at 3793.772 kb on + strand at 3793.772 kb on + strand at 3793.773 kb on - strand at 3794.100 kb on + strand at 3794.101 kb on - strand at 3794.101 kb on - strand at 3794.102 kb on + strand at 3794.103 kb on - strand at 3794.103 kb on - strand at 3794.103 kb on - strand at 3794.103 kb on - strand at 3794.103 kb on - strand at 3794.103 kb on - strand at 3794.459 kb on - strand, within Psyr_3165 at 3794.459 kb on - strand, within Psyr_3165 at 3794.461 kb on + strand, within Psyr_3165 at 3794.461 kb on + strand, within Psyr_3165 at 3794.461 kb on + strand, within Psyr_3165 at 3794.461 kb on + strand, within Psyr_3165 at 3794.461 kb on + strand, within Psyr_3165 at 3794.461 kb on + strand, within Psyr_3165 at 3794.461 kb on + strand, within Psyr_3165 at 3794.461 kb on + strand, within Psyr_3165 at 3794.461 kb on + strand, within Psyr_3165 at 3794.462 kb on - strand, within Psyr_3165 at 3794.462 kb on - strand, within Psyr_3165 at 3794.462 kb on - strand, within Psyr_3165 at 3794.462 kb on - strand, within Psyr_3165 at 3794.462 kb on - strand, within Psyr_3165 at 3794.462 kb on - strand, within Psyr_3165 at 3794.644 kb on + strand, within Psyr_3165 at 3794.648 kb on + strand, within Psyr_3165 at 3794.648 kb on + strand, within Psyr_3165 at 3794.649 kb on - strand, within Psyr_3165 at 3794.649 kb on - strand, within Psyr_3165 at 3794.680 kb on - strand, within Psyr_3165 at 3794.710 kb on + strand, within Psyr_3165 at 3794.711 kb on - strand, within Psyr_3165 at 3794.722 kb on + strand, within Psyr_3165 at 3794.722 kb on + strand, within Psyr_3165 at 3794.722 kb on + strand, within Psyr_3165 at 3794.722 kb on + strand, within Psyr_3165 at 3794.722 kb on + strand, within Psyr_3165 at 3794.723 kb on - strand, within Psyr_3165 at 3794.723 kb on - strand, within Psyr_3165 at 3794.723 kb on - strand, within Psyr_3165 at 3794.723 kb on - strand, within Psyr_3165 at 3794.723 kb on - strand, within Psyr_3165 at 3794.723 kb on - strand, within Psyr_3165 at 3794.723 kb on - strand, within Psyr_3165 at 3794.951 kb on + strand, within Psyr_3165 at 3794.952 kb on - strand, within Psyr_3165 at 3794.952 kb on - strand, within Psyr_3165 at 3794.952 kb on - strand, within Psyr_3165 at 3795.098 kb on + strand at 3795.254 kb on + strand, within Psyr_3166 at 3795.254 kb on + strand, within Psyr_3166 at 3795.362 kb on + strand, within Psyr_3166 at 3795.362 kb on + strand, within Psyr_3166 at 3795.362 kb on + strand, within Psyr_3166 at 3795.362 kb on + strand, within Psyr_3166 at 3795.362 kb on + strand at 3795.363 kb on - strand, within Psyr_3166 at 3795.363 kb on - strand, within Psyr_3166 at 3795.386 kb on + strand, within Psyr_3166 at 3795.387 kb on - strand, within Psyr_3166 at 3795.421 kb on - strand, within Psyr_3166 at 3795.421 kb on - strand, within Psyr_3166 at 3795.517 kb on + strand, within Psyr_3166 at 3795.518 kb on - strand, within Psyr_3166 at 3795.518 kb on - strand, within Psyr_3166 at 3795.518 kb on - strand, within Psyr_3166 at 3795.695 kb on + strand, within Psyr_3166 at 3795.705 kb on + strand, within Psyr_3166 at 3795.705 kb on + strand, within Psyr_3166 at 3795.800 kb on + strand at 3795.801 kb on - strand at 3795.801 kb on - strand at 3795.801 kb on - strand at 3795.813 kb on + strand at 3795.814 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 125 ug/ml remove 3,793,170 + -1.4 3,793,170 + -2.9 3,793,172 + -2.8 3,793,172 + -1.3 3,793,172 + -2.8 3,793,172 + -1.3 3,793,172 + -1.0 3,793,173 - -0.0 3,793,173 - +0.0 3,793,173 - -1.3 3,793,173 - -0.7 3,793,173 - -2.3 3,793,180 + -1.2 3,793,180 + -1.9 3,793,181 - +0.2 3,793,181 - +0.1 3,793,181 - -0.6 3,793,181 - +0.6 3,793,181 - -0.4 3,793,184 + -1.0 3,793,184 + -1.4 3,793,184 + -1.0 3,793,184 + -1.9 3,793,184 + -1.0 3,793,184 + -0.4 3,793,184 + -0.7 3,793,185 - Psyr_3164 0.10 +0.8 3,793,185 - Psyr_3164 0.10 -2.6 3,793,185 - Psyr_3164 0.10 +0.2 3,793,185 - Psyr_3164 0.10 +0.6 3,793,185 - Psyr_3164 0.10 -0.4 3,793,185 - Psyr_3164 0.10 +0.5 3,793,185 - Psyr_3164 0.10 -0.2 3,793,185 - Psyr_3164 0.10 -1.8 3,793,208 + Psyr_3164 0.14 -1.4 3,793,208 + Psyr_3164 0.14 -4.3 3,793,208 + Psyr_3164 0.14 -2.0 3,793,208 + Psyr_3164 0.14 -1.8 3,793,208 + Psyr_3164 0.14 -0.3 3,793,209 - Psyr_3164 0.14 +0.4 3,793,209 - Psyr_3164 0.14 -1.4 3,793,209 - Psyr_3164 0.14 -0.9 3,793,209 - Psyr_3164 0.14 +3.1 3,793,209 - Psyr_3164 0.14 +0.1 3,793,212 + Psyr_3164 0.14 -2.2 3,793,213 - Psyr_3164 0.14 -0.9 3,793,213 - Psyr_3164 0.14 +0.3 3,793,334 - Psyr_3164 0.32 -1.1 3,793,408 + Psyr_3164 0.43 -0.3 3,793,408 + Psyr_3164 0.43 +0.3 3,793,408 + Psyr_3164 0.43 -0.5 3,793,408 + Psyr_3164 0.43 -0.9 3,793,408 + Psyr_3164 0.43 -2.8 3,793,408 + Psyr_3164 0.43 -0.3 3,793,408 + Psyr_3164 0.43 -2.6 3,793,408 + Psyr_3164 0.43 -2.3 3,793,409 - Psyr_3164 0.43 +0.7 3,793,409 - Psyr_3164 0.43 +0.6 3,793,409 - Psyr_3164 0.43 -0.6 3,793,412 + Psyr_3164 0.44 +0.1 3,793,412 + Psyr_3164 0.44 -1.6 3,793,412 + Psyr_3164 0.44 -1.3 3,793,413 - Psyr_3164 0.44 -1.4 3,793,604 + Psyr_3164 0.72 -2.0 3,793,604 + Psyr_3164 0.72 -1.6 3,793,605 - Psyr_3164 0.73 +0.2 3,793,605 - Psyr_3164 0.73 +0.6 3,793,709 + Psyr_3164 0.88 +0.8 3,793,709 + Psyr_3164 0.88 -1.6 3,793,710 - Psyr_3164 0.88 +0.4 3,793,713 + Psyr_3164 0.89 -0.4 3,793,713 + Psyr_3164 0.89 +0.0 3,793,713 + Psyr_3164 0.89 -0.7 3,793,713 + Psyr_3164 0.89 -0.0 3,793,714 - Psyr_3164 0.89 -2.6 3,793,765 - +1.7 3,793,772 + +1.0 3,793,772 + -1.1 3,793,773 - -1.6 3,794,100 + +0.2 3,794,101 - -0.0 3,794,101 - -0.3 3,794,102 + -1.1 3,794,103 - -0.4 3,794,103 - -0.2 3,794,103 - -0.4 3,794,103 - -0.0 3,794,103 - +0.9 3,794,103 - -0.4 3,794,459 - Psyr_3165 0.35 -0.9 3,794,459 - Psyr_3165 0.35 -0.4 3,794,461 + Psyr_3165 0.35 +0.2 3,794,461 + Psyr_3165 0.35 +0.8 3,794,461 + Psyr_3165 0.35 +0.4 3,794,461 + Psyr_3165 0.35 +0.7 3,794,461 + Psyr_3165 0.35 +0.6 3,794,461 + Psyr_3165 0.35 -0.8 3,794,461 + Psyr_3165 0.35 +0.3 3,794,461 + Psyr_3165 0.35 +1.6 3,794,461 + Psyr_3165 0.35 +1.6 3,794,462 - Psyr_3165 0.35 -1.4 3,794,462 - Psyr_3165 0.35 +0.4 3,794,462 - Psyr_3165 0.35 +0.1 3,794,462 - Psyr_3165 0.35 -0.3 3,794,462 - Psyr_3165 0.35 -1.0 3,794,462 - Psyr_3165 0.35 +0.2 3,794,644 + Psyr_3165 0.55 +0.2 3,794,648 + Psyr_3165 0.56 +0.4 3,794,648 + Psyr_3165 0.56 +0.6 3,794,649 - Psyr_3165 0.56 -0.4 3,794,649 - Psyr_3165 0.56 +0.6 3,794,680 - Psyr_3165 0.59 +0.4 3,794,710 + Psyr_3165 0.63 +1.6 3,794,711 - Psyr_3165 0.63 +0.8 3,794,722 + Psyr_3165 0.64 +0.5 3,794,722 + Psyr_3165 0.64 -0.0 3,794,722 + Psyr_3165 0.64 +1.2 3,794,722 + Psyr_3165 0.64 +1.3 3,794,722 + Psyr_3165 0.64 +1.1 3,794,723 - Psyr_3165 0.64 +0.7 3,794,723 - Psyr_3165 0.64 +0.3 3,794,723 - Psyr_3165 0.64 +0.3 3,794,723 - Psyr_3165 0.64 +1.0 3,794,723 - Psyr_3165 0.64 -2.0 3,794,723 - Psyr_3165 0.64 -0.4 3,794,723 - Psyr_3165 0.64 -0.7 3,794,951 + Psyr_3165 0.89 -0.4 3,794,952 - Psyr_3165 0.89 +1.0 3,794,952 - Psyr_3165 0.89 +0.6 3,794,952 - Psyr_3165 0.89 +1.7 3,795,098 + -0.9 3,795,254 + Psyr_3166 0.28 -1.6 3,795,254 + Psyr_3166 0.28 +2.1 3,795,362 + Psyr_3166 0.42 -0.4 3,795,362 + Psyr_3166 0.42 +1.6 3,795,362 + Psyr_3166 0.42 -1.1 3,795,362 + Psyr_3166 0.42 -0.4 3,795,362 + -0.4 3,795,363 - Psyr_3166 0.42 +1.4 3,795,363 - Psyr_3166 0.42 +0.1 3,795,386 + Psyr_3166 0.45 -0.4 3,795,387 - Psyr_3166 0.45 -0.2 3,795,421 - Psyr_3166 0.50 +0.6 3,795,421 - Psyr_3166 0.50 +1.1 3,795,517 + Psyr_3166 0.62 +0.1 3,795,518 - Psyr_3166 0.62 -0.1 3,795,518 - Psyr_3166 0.62 +0.6 3,795,518 - Psyr_3166 0.62 -1.0 3,795,695 + Psyr_3166 0.85 -1.0 3,795,705 + Psyr_3166 0.86 -0.4 3,795,705 + Psyr_3166 0.86 +0.5 3,795,800 + -1.3 3,795,801 - +0.7 3,795,801 - -0.2 3,795,801 - -0.1 3,795,813 + -1.1 3,795,814 - +1.9
Or see this region's nucleotide sequence