Experiment: KB with Nitrofurantoin 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3059 and Psyr_3060 are separated by 450 nucleotides Psyr_3060 and Psyr_3061 are separated by 109 nucleotides Psyr_3061 and Psyr_3062 are separated by 42 nucleotides
Psyr_3059: Psyr_3059 - Hemolysin-type calcium-binding region:Peptidase M10A and M12B, matrixin and adamalysin, at 3,657,318 to 3,658,925
_3059
Psyr_3060: Psyr_3060 - GAF:ATP-binding region, ATPase-like:Histidine kinase A, N-terminal, at 3,659,376 to 3,660,704
_3060
Psyr_3061: Psyr_3061 - putative glutathione S-transferase, at 3,660,814 to 3,661,461
_3061
Psyr_3062: Psyr_3062 - transcriptional regulator, LysR family, at 3,661,504 to 3,662,400
_3062
Position (kb)
3659
3660
3661 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3658.414 kb on + strand, within Psyr_3059 at 3658.415 kb on - strand, within Psyr_3059 at 3658.480 kb on + strand, within Psyr_3059 at 3658.481 kb on - strand at 3658.481 kb on - strand, within Psyr_3059 at 3658.632 kb on - strand, within Psyr_3059 at 3658.762 kb on + strand, within Psyr_3059 at 3658.762 kb on + strand, within Psyr_3059 at 3658.763 kb on - strand, within Psyr_3059 at 3658.763 kb on - strand, within Psyr_3059 at 3658.763 kb on - strand, within Psyr_3059 at 3658.801 kb on + strand at 3658.802 kb on - strand at 3658.802 kb on - strand at 3658.945 kb on + strand at 3658.955 kb on - strand at 3659.032 kb on - strand at 3659.131 kb on + strand at 3659.148 kb on - strand at 3659.149 kb on + strand at 3659.149 kb on + strand at 3659.175 kb on + strand at 3659.299 kb on + strand at 3659.300 kb on - strand at 3659.300 kb on - strand at 3659.300 kb on - strand at 3659.308 kb on + strand at 3659.308 kb on + strand at 3659.309 kb on - strand at 3659.309 kb on - strand at 3659.309 kb on - strand at 3659.326 kb on - strand at 3659.334 kb on + strand at 3659.335 kb on - strand at 3659.344 kb on - strand at 3659.344 kb on - strand at 3659.344 kb on - strand at 3659.344 kb on - strand at 3659.344 kb on - strand at 3659.350 kb on + strand at 3659.350 kb on + strand at 3659.350 kb on + strand at 3659.351 kb on - strand at 3659.351 kb on - strand at 3659.351 kb on - strand at 3659.351 kb on - strand at 3659.446 kb on + strand at 3659.709 kb on + strand, within Psyr_3060 at 3659.709 kb on + strand, within Psyr_3060 at 3659.710 kb on - strand, within Psyr_3060 at 3659.710 kb on - strand, within Psyr_3060 at 3659.710 kb on - strand, within Psyr_3060 at 3659.710 kb on - strand, within Psyr_3060 at 3659.710 kb on - strand at 3659.710 kb on - strand, within Psyr_3060 at 3659.721 kb on + strand, within Psyr_3060 at 3659.742 kb on - strand, within Psyr_3060 at 3659.784 kb on - strand, within Psyr_3060 at 3660.386 kb on + strand, within Psyr_3060 at 3660.386 kb on + strand, within Psyr_3060 at 3660.386 kb on + strand, within Psyr_3060 at 3660.387 kb on - strand, within Psyr_3060 at 3660.387 kb on - strand, within Psyr_3060 at 3660.449 kb on - strand, within Psyr_3060 at 3660.580 kb on + strand at 3660.642 kb on + strand at 3660.653 kb on - strand at 3660.674 kb on + strand at 3660.689 kb on + strand at 3660.692 kb on - strand at 3660.733 kb on + strand at 3660.853 kb on + strand at 3660.853 kb on + strand at 3660.890 kb on - strand, within Psyr_3061 at 3661.457 kb on + strand at 3661.457 kb on + strand at 3661.457 kb on + strand at 3661.457 kb on + strand at 3661.458 kb on - strand at 3661.458 kb on - strand at 3661.458 kb on - strand at 3661.458 kb on - strand at 3661.458 kb on - strand at 3661.458 kb on - strand at 3661.458 kb on - strand at 3661.458 kb on - strand at 3661.458 kb on - strand at 3661.458 kb on - strand at 3661.458 kb on - strand at 3661.461 kb on + strand at 3661.461 kb on + strand at 3661.462 kb on - strand at 3661.462 kb on - strand at 3661.508 kb on - strand at 3661.622 kb on + strand, within Psyr_3062 at 3661.623 kb on - strand, within Psyr_3062 at 3661.623 kb on - strand, within Psyr_3062
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 125 ug/ml remove 3,658,414 + Psyr_3059 0.68 +0.3 3,658,415 - Psyr_3059 0.68 -0.4 3,658,480 + Psyr_3059 0.72 +0.4 3,658,481 - +0.6 3,658,481 - Psyr_3059 0.72 +1.2 3,658,632 - Psyr_3059 0.82 -1.2 3,658,762 + Psyr_3059 0.90 -0.1 3,658,762 + Psyr_3059 0.90 +1.8 3,658,763 - Psyr_3059 0.90 +0.2 3,658,763 - Psyr_3059 0.90 -0.7 3,658,763 - Psyr_3059 0.90 +0.9 3,658,801 + +1.2 3,658,802 - +1.4 3,658,802 - -0.0 3,658,945 + -0.8 3,658,955 - +0.5 3,659,032 - -1.2 3,659,131 + -1.4 3,659,148 - +0.9 3,659,149 + -1.4 3,659,149 + +0.1 3,659,175 + +0.6 3,659,299 + +0.1 3,659,300 - -0.5 3,659,300 - +0.8 3,659,300 - +1.0 3,659,308 + -0.3 3,659,308 + -0.7 3,659,309 - +0.3 3,659,309 - -0.2 3,659,309 - +0.8 3,659,326 - +0.3 3,659,334 + -0.4 3,659,335 - +0.0 3,659,344 - -1.4 3,659,344 - +1.9 3,659,344 - +2.4 3,659,344 - +0.5 3,659,344 - +1.4 3,659,350 + -1.4 3,659,350 + +0.3 3,659,350 + +0.3 3,659,351 - -0.1 3,659,351 - -1.2 3,659,351 - +1.6 3,659,351 - -1.2 3,659,446 + +0.8 3,659,709 + Psyr_3060 0.25 +0.6 3,659,709 + Psyr_3060 0.25 -0.2 3,659,710 - Psyr_3060 0.25 -0.2 3,659,710 - Psyr_3060 0.25 -0.2 3,659,710 - Psyr_3060 0.25 -0.1 3,659,710 - Psyr_3060 0.25 -0.3 3,659,710 - +0.6 3,659,710 - Psyr_3060 0.25 -0.4 3,659,721 + Psyr_3060 0.26 -0.7 3,659,742 - Psyr_3060 0.28 -0.4 3,659,784 - Psyr_3060 0.31 +0.6 3,660,386 + Psyr_3060 0.76 -0.8 3,660,386 + Psyr_3060 0.76 -1.0 3,660,386 + Psyr_3060 0.76 -1.4 3,660,387 - Psyr_3060 0.76 -1.2 3,660,387 - Psyr_3060 0.76 -0.1 3,660,449 - Psyr_3060 0.81 -1.8 3,660,580 + -0.8 3,660,642 + +0.6 3,660,653 - -0.0 3,660,674 + +0.7 3,660,689 + -0.6 3,660,692 - -0.3 3,660,733 + +1.1 3,660,853 + -2.1 3,660,853 + -0.6 3,660,890 - Psyr_3061 0.12 -0.3 3,661,457 + -0.6 3,661,457 + +1.2 3,661,457 + -0.2 3,661,457 + +0.6 3,661,458 - +1.2 3,661,458 - -1.4 3,661,458 - -1.3 3,661,458 - -1.6 3,661,458 - -1.2 3,661,458 - -0.2 3,661,458 - +0.3 3,661,458 - -0.0 3,661,458 - +0.9 3,661,458 - +1.3 3,661,458 - -0.8 3,661,461 + -0.0 3,661,461 + -0.0 3,661,462 - -0.1 3,661,462 - +0.6 3,661,508 - -2.1 3,661,622 + Psyr_3062 0.13 -0.2 3,661,623 - Psyr_3062 0.13 -2.4 3,661,623 - Psyr_3062 0.13 -0.5
Or see this region's nucleotide sequence