Experiment: KB with Nitrofurantoin 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2981 and Psyr_2982 are separated by 55 nucleotides Psyr_2982 and Psyr_2983 are separated by 167 nucleotides Psyr_2983 and Psyr_2984 are separated by 14 nucleotides Psyr_2984 and Psyr_2985 are separated by 24 nucleotides Psyr_2985 and Psyr_2986 are separated by 0 nucleotides
Psyr_2981: Psyr_2981 - hypothetical protein, at 3,574,664 to 3,574,945
_2981
Psyr_2982: Psyr_2982 - conserved hypothetical protein, at 3,575,001 to 3,575,354
_2982
Psyr_2983: Psyr_2983 - conserved domain protein, at 3,575,522 to 3,575,827
_2983
Psyr_2984: Psyr_2984 - conserved hypothetical protein, at 3,575,842 to 3,576,024
_2984
Psyr_2985: Psyr_2985 - DNA-N1-methyladenine dioxygenase, at 3,576,049 to 3,576,735
_2985
Psyr_2986: Psyr_2986 - Glycoside hydrolase, family 15, at 3,576,736 to 3,578,553
_2986
Position (kb)
3575
3576 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3574.522 kb on - strand at 3574.522 kb on - strand at 3574.522 kb on - strand at 3574.522 kb on - strand at 3574.522 kb on - strand at 3574.573 kb on + strand at 3574.573 kb on + strand at 3574.574 kb on - strand at 3574.574 kb on - strand at 3574.574 kb on - strand at 3574.605 kb on + strand at 3574.605 kb on + strand at 3574.605 kb on + strand at 3574.605 kb on + strand at 3574.605 kb on + strand at 3574.606 kb on - strand at 3574.606 kb on - strand at 3574.609 kb on - strand at 3574.646 kb on + strand at 3574.730 kb on + strand, within Psyr_2981 at 3574.730 kb on + strand, within Psyr_2981 at 3574.943 kb on + strand at 3574.944 kb on - strand at 3575.118 kb on + strand, within Psyr_2982 at 3575.282 kb on + strand, within Psyr_2982 at 3575.282 kb on + strand, within Psyr_2982 at 3575.282 kb on + strand, within Psyr_2982 at 3575.282 kb on + strand, within Psyr_2982 at 3575.282 kb on + strand, within Psyr_2982 at 3575.283 kb on - strand, within Psyr_2982 at 3575.283 kb on - strand, within Psyr_2982 at 3575.283 kb on - strand, within Psyr_2982 at 3575.334 kb on + strand at 3575.334 kb on + strand at 3575.337 kb on + strand at 3575.337 kb on + strand at 3575.337 kb on + strand at 3575.338 kb on - strand at 3575.338 kb on - strand at 3575.369 kb on + strand at 3575.369 kb on + strand at 3575.370 kb on - strand at 3575.370 kb on - strand at 3575.379 kb on - strand at 3575.399 kb on + strand at 3575.399 kb on + strand at 3575.399 kb on + strand at 3575.400 kb on - strand at 3575.400 kb on - strand at 3575.400 kb on - strand at 3575.442 kb on - strand at 3575.442 kb on - strand at 3575.533 kb on - strand at 3575.533 kb on - strand at 3575.596 kb on + strand, within Psyr_2983 at 3575.654 kb on + strand, within Psyr_2983 at 3575.654 kb on + strand, within Psyr_2983 at 3575.654 kb on + strand, within Psyr_2983 at 3575.654 kb on + strand, within Psyr_2983 at 3575.654 kb on + strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.655 kb on - strand, within Psyr_2983 at 3575.884 kb on + strand, within Psyr_2984 at 3575.991 kb on + strand, within Psyr_2984 at 3575.991 kb on + strand, within Psyr_2984 at 3575.992 kb on - strand, within Psyr_2984 at 3575.992 kb on - strand, within Psyr_2984 at 3576.025 kb on + strand at 3576.025 kb on + strand at 3576.368 kb on + strand, within Psyr_2985 at 3576.368 kb on + strand, within Psyr_2985 at 3576.368 kb on + strand, within Psyr_2985 at 3576.451 kb on - strand, within Psyr_2985 at 3576.451 kb on - strand, within Psyr_2985 at 3576.493 kb on + strand, within Psyr_2985 at 3576.782 kb on - strand at 3576.782 kb on - strand at 3576.807 kb on + strand at 3576.807 kb on + strand at 3576.807 kb on + strand at 3576.808 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 125 ug/ml remove 3,574,522 - -0.3 3,574,522 - -1.0 3,574,522 - +0.4 3,574,522 - +0.9 3,574,522 - -0.2 3,574,573 + +0.4 3,574,573 + +0.8 3,574,574 - -2.8 3,574,574 - -1.4 3,574,574 - -0.7 3,574,605 + +0.6 3,574,605 + +0.5 3,574,605 + -0.4 3,574,605 + +0.6 3,574,605 + -0.2 3,574,606 - -0.8 3,574,606 - -0.2 3,574,609 - +0.1 3,574,646 + -0.2 3,574,730 + Psyr_2981 0.23 -0.1 3,574,730 + Psyr_2981 0.23 +0.5 3,574,943 + -1.0 3,574,944 - -0.4 3,575,118 + Psyr_2982 0.33 +0.3 3,575,282 + Psyr_2982 0.79 -0.4 3,575,282 + Psyr_2982 0.79 +2.9 3,575,282 + Psyr_2982 0.79 -0.4 3,575,282 + Psyr_2982 0.79 +1.0 3,575,282 + Psyr_2982 0.79 +0.4 3,575,283 - Psyr_2982 0.80 -0.2 3,575,283 - Psyr_2982 0.80 +1.0 3,575,283 - Psyr_2982 0.80 -0.2 3,575,334 + -0.4 3,575,334 + -1.4 3,575,337 + +1.0 3,575,337 + -0.4 3,575,337 + -0.2 3,575,338 - -1.1 3,575,338 - -0.2 3,575,369 + -0.1 3,575,369 + -1.6 3,575,370 - +0.2 3,575,370 - +0.4 3,575,379 - -0.2 3,575,399 + +1.2 3,575,399 + -0.3 3,575,399 + +0.0 3,575,400 - -1.7 3,575,400 - -1.4 3,575,400 - +0.9 3,575,442 - -1.4 3,575,442 - +0.3 3,575,533 - +0.6 3,575,533 - +0.9 3,575,596 + Psyr_2983 0.24 -0.2 3,575,654 + Psyr_2983 0.43 -1.2 3,575,654 + Psyr_2983 0.43 +0.3 3,575,654 + Psyr_2983 0.43 +0.6 3,575,654 + Psyr_2983 0.43 -0.4 3,575,654 + Psyr_2983 0.43 +0.1 3,575,655 - Psyr_2983 0.43 +0.6 3,575,655 - Psyr_2983 0.43 +0.6 3,575,655 - Psyr_2983 0.43 -0.7 3,575,655 - Psyr_2983 0.43 +0.8 3,575,655 - Psyr_2983 0.43 -0.2 3,575,655 - Psyr_2983 0.43 -0.1 3,575,655 - Psyr_2983 0.43 -0.3 3,575,655 - Psyr_2983 0.43 +1.2 3,575,655 - Psyr_2983 0.43 +1.6 3,575,655 - Psyr_2983 0.43 -0.2 3,575,655 - Psyr_2983 0.43 -0.4 3,575,884 + Psyr_2984 0.23 -0.9 3,575,991 + Psyr_2984 0.81 +0.0 3,575,991 + Psyr_2984 0.81 -0.1 3,575,992 - Psyr_2984 0.82 +2.4 3,575,992 - Psyr_2984 0.82 -0.1 3,576,025 + +1.2 3,576,025 + +0.6 3,576,368 + Psyr_2985 0.46 +1.9 3,576,368 + Psyr_2985 0.46 -1.7 3,576,368 + Psyr_2985 0.46 -1.0 3,576,451 - Psyr_2985 0.59 +0.9 3,576,451 - Psyr_2985 0.59 -2.0 3,576,493 + Psyr_2985 0.65 -1.0 3,576,782 - -1.7 3,576,782 - -1.1 3,576,807 + -2.0 3,576,807 + -0.4 3,576,807 + -0.7 3,576,808 - +0.4
Or see this region's nucleotide sequence