Experiment: KB with Nitrofurantoin 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2171 and Psyr_2172 overlap by 4 nucleotides Psyr_2172 and Psyr_2173 are separated by 25 nucleotides Psyr_2173 and Psyr_2174 are separated by 0 nucleotides
Psyr_2171: Psyr_2171 - HpcH/HpaI aldolase, at 2,524,631 to 2,525,446
_2171
Psyr_2172: Psyr_2172 - conserved hypothetical protein, at 2,525,443 to 2,526,288
_2172
Psyr_2173: Psyr_2173 - Electron transfer flavoprotein, alpha subunit, at 2,526,314 to 2,527,243
_2173
Psyr_2174: Psyr_2174 - Electron transfer flavoprotein beta-subunit, at 2,527,244 to 2,527,993
_2174
Position (kb)
2525
2526
2527 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2524.456 kb on + strand at 2524.601 kb on + strand at 2524.601 kb on + strand at 2524.602 kb on - strand at 2524.602 kb on - strand at 2524.602 kb on - strand at 2524.608 kb on + strand at 2524.608 kb on + strand at 2524.608 kb on + strand at 2524.608 kb on + strand at 2524.609 kb on - strand at 2524.609 kb on - strand at 2524.813 kb on + strand, within Psyr_2171 at 2524.862 kb on + strand, within Psyr_2171 at 2524.863 kb on - strand, within Psyr_2171 at 2524.865 kb on + strand, within Psyr_2171 at 2525.671 kb on + strand, within Psyr_2172 at 2525.671 kb on + strand, within Psyr_2172 at 2525.671 kb on + strand, within Psyr_2172 at 2525.671 kb on + strand, within Psyr_2172 at 2525.672 kb on - strand, within Psyr_2172 at 2525.672 kb on - strand, within Psyr_2172 at 2525.672 kb on - strand, within Psyr_2172 at 2525.672 kb on - strand, within Psyr_2172 at 2525.708 kb on + strand, within Psyr_2172 at 2525.708 kb on + strand, within Psyr_2172 at 2525.709 kb on - strand, within Psyr_2172 at 2525.709 kb on - strand, within Psyr_2172 at 2525.709 kb on - strand, within Psyr_2172 at 2525.720 kb on + strand, within Psyr_2172 at 2525.721 kb on - strand, within Psyr_2172 at 2525.727 kb on + strand, within Psyr_2172 at 2525.728 kb on - strand, within Psyr_2172 at 2525.728 kb on - strand, within Psyr_2172 at 2525.915 kb on - strand at 2525.929 kb on + strand, within Psyr_2172 at 2525.978 kb on - strand, within Psyr_2172 at 2526.004 kb on + strand, within Psyr_2172 at 2526.004 kb on + strand, within Psyr_2172 at 2526.288 kb on + strand at 2526.288 kb on + strand at 2526.288 kb on + strand at 2526.288 kb on + strand at 2526.470 kb on - strand, within Psyr_2173 at 2526.483 kb on + strand, within Psyr_2173 at 2526.483 kb on + strand, within Psyr_2173 at 2526.483 kb on + strand, within Psyr_2173 at 2526.483 kb on + strand, within Psyr_2173 at 2526.483 kb on + strand, within Psyr_2173 at 2526.483 kb on + strand, within Psyr_2173 at 2526.484 kb on - strand, within Psyr_2173 at 2526.484 kb on - strand, within Psyr_2173 at 2526.484 kb on - strand, within Psyr_2173 at 2526.484 kb on - strand, within Psyr_2173 at 2526.484 kb on - strand, within Psyr_2173 at 2526.484 kb on - strand, within Psyr_2173 at 2526.484 kb on - strand, within Psyr_2173 at 2526.841 kb on + strand, within Psyr_2173 at 2526.841 kb on + strand, within Psyr_2173 at 2526.862 kb on - strand, within Psyr_2173 at 2526.862 kb on - strand, within Psyr_2173 at 2526.993 kb on + strand, within Psyr_2173 at 2527.014 kb on - strand, within Psyr_2173 at 2527.246 kb on - strand at 2527.251 kb on - strand at 2527.251 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 125 ug/ml remove 2,524,456 + -1.1 2,524,601 + +0.3 2,524,601 + -0.1 2,524,602 - -0.1 2,524,602 - -2.3 2,524,602 - +1.4 2,524,608 + -0.2 2,524,608 + +0.6 2,524,608 + +0.2 2,524,608 + +0.6 2,524,609 - +0.3 2,524,609 - -0.3 2,524,813 + Psyr_2171 0.22 +0.7 2,524,862 + Psyr_2171 0.28 +0.6 2,524,863 - Psyr_2171 0.28 +0.1 2,524,865 + Psyr_2171 0.29 -1.5 2,525,671 + Psyr_2172 0.27 +1.2 2,525,671 + Psyr_2172 0.27 +0.6 2,525,671 + Psyr_2172 0.27 -1.2 2,525,671 + Psyr_2172 0.27 -0.5 2,525,672 - Psyr_2172 0.27 -0.4 2,525,672 - Psyr_2172 0.27 -0.2 2,525,672 - Psyr_2172 0.27 -0.5 2,525,672 - Psyr_2172 0.27 +0.6 2,525,708 + Psyr_2172 0.31 -0.2 2,525,708 + Psyr_2172 0.31 -0.4 2,525,709 - Psyr_2172 0.31 -0.6 2,525,709 - Psyr_2172 0.31 -0.4 2,525,709 - Psyr_2172 0.31 -1.3 2,525,720 + Psyr_2172 0.33 +0.4 2,525,721 - Psyr_2172 0.33 -1.4 2,525,727 + Psyr_2172 0.34 -0.4 2,525,728 - Psyr_2172 0.34 -0.8 2,525,728 - Psyr_2172 0.34 -1.4 2,525,915 - +0.6 2,525,929 + Psyr_2172 0.57 -1.0 2,525,978 - Psyr_2172 0.63 -0.1 2,526,004 + Psyr_2172 0.66 -0.0 2,526,004 + Psyr_2172 0.66 -1.4 2,526,288 + -2.2 2,526,288 + -0.0 2,526,288 + +1.9 2,526,288 + +0.9 2,526,470 - Psyr_2173 0.17 +0.6 2,526,483 + Psyr_2173 0.18 +0.6 2,526,483 + Psyr_2173 0.18 +0.0 2,526,483 + Psyr_2173 0.18 -1.0 2,526,483 + Psyr_2173 0.18 +0.6 2,526,483 + Psyr_2173 0.18 -0.6 2,526,483 + Psyr_2173 0.18 +0.4 2,526,484 - Psyr_2173 0.18 +0.6 2,526,484 - Psyr_2173 0.18 -2.2 2,526,484 - Psyr_2173 0.18 -0.4 2,526,484 - Psyr_2173 0.18 -0.1 2,526,484 - Psyr_2173 0.18 -0.6 2,526,484 - Psyr_2173 0.18 +0.8 2,526,484 - Psyr_2173 0.18 +0.6 2,526,841 + Psyr_2173 0.57 -0.2 2,526,841 + Psyr_2173 0.57 -1.0 2,526,862 - Psyr_2173 0.59 +1.6 2,526,862 - Psyr_2173 0.59 -0.2 2,526,993 + Psyr_2173 0.73 +1.0 2,527,014 - Psyr_2173 0.75 +1.0 2,527,246 - -0.1 2,527,251 - -0.7 2,527,251 - -0.6
Or see this region's nucleotide sequence