Experiment: KB with Nitrofurantoin 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2131 and Psyr_2132 are separated by 3 nucleotides Psyr_2132 and Psyr_2133 are separated by 135 nucleotides Psyr_2133 and Psyr_2134 are separated by 77 nucleotides Psyr_2134 and Psyr_2135 are separated by 96 nucleotides
Psyr_2131: Psyr_2131 - transcriptional regulator, TetR family, at 2,491,504 to 2,492,160
_2131
Psyr_2132: Psyr_2132 - shikimate dehydrogenase, at 2,492,164 to 2,493,018
_2132
Psyr_2133: Psyr_2133 - Pseudouridine synthase, at 2,493,154 to 2,494,095
_2133
Psyr_2134: Psyr_2134 - protein of unknown function DUF903, at 2,494,173 to 2,494,397
_2134
Psyr_2135: Psyr_2135 - molybdenum cofactor guanylyltransferase, at 2,494,494 to 2,495,099
_2135
Position (kb)
2493
2494
2495 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2492.166 kb on - strand at 2492.166 kb on - strand at 2492.166 kb on - strand at 2492.166 kb on - strand at 2492.166 kb on - strand at 2492.327 kb on + strand, within Psyr_2132 at 2492.328 kb on - strand, within Psyr_2132 at 2492.415 kb on + strand, within Psyr_2132 at 2492.620 kb on + strand, within Psyr_2132 at 2492.621 kb on - strand, within Psyr_2132 at 2492.621 kb on - strand, within Psyr_2132 at 2492.621 kb on - strand, within Psyr_2132 at 2492.725 kb on - strand, within Psyr_2132 at 2493.024 kb on + strand at 2493.024 kb on + strand at 2493.024 kb on + strand at 2493.024 kb on + strand at 2493.025 kb on - strand at 2493.025 kb on - strand at 2493.025 kb on - strand at 2493.025 kb on - strand at 2493.158 kb on + strand at 2493.185 kb on - strand at 2493.185 kb on - strand at 2493.247 kb on - strand at 2493.247 kb on - strand at 2493.253 kb on + strand, within Psyr_2133 at 2493.253 kb on + strand, within Psyr_2133 at 2493.253 kb on + strand, within Psyr_2133 at 2493.254 kb on - strand, within Psyr_2133 at 2493.254 kb on - strand, within Psyr_2133 at 2493.254 kb on - strand, within Psyr_2133 at 2493.254 kb on - strand, within Psyr_2133 at 2493.421 kb on + strand, within Psyr_2133 at 2493.541 kb on + strand, within Psyr_2133 at 2493.541 kb on + strand, within Psyr_2133 at 2493.542 kb on - strand, within Psyr_2133 at 2493.594 kb on + strand, within Psyr_2133 at 2493.595 kb on - strand, within Psyr_2133 at 2493.595 kb on - strand, within Psyr_2133 at 2493.595 kb on - strand, within Psyr_2133 at 2493.674 kb on - strand, within Psyr_2133 at 2493.917 kb on + strand, within Psyr_2133 at 2493.918 kb on - strand, within Psyr_2133 at 2493.918 kb on - strand, within Psyr_2133 at 2493.918 kb on - strand, within Psyr_2133 at 2493.918 kb on - strand, within Psyr_2133 at 2494.097 kb on - strand at 2494.190 kb on + strand at 2494.190 kb on + strand at 2494.191 kb on - strand at 2494.194 kb on + strand at 2494.194 kb on + strand at 2494.195 kb on - strand at 2494.195 kb on - strand at 2494.268 kb on - strand, within Psyr_2134 at 2494.268 kb on - strand, within Psyr_2134 at 2494.398 kb on + strand at 2494.466 kb on - strand at 2494.467 kb on + strand at 2494.467 kb on + strand at 2494.676 kb on + strand, within Psyr_2135 at 2494.677 kb on - strand, within Psyr_2135 at 2494.680 kb on + strand, within Psyr_2135 at 2494.785 kb on + strand, within Psyr_2135 at 2494.785 kb on + strand, within Psyr_2135 at 2494.842 kb on + strand, within Psyr_2135 at 2494.842 kb on + strand, within Psyr_2135 at 2494.843 kb on - strand, within Psyr_2135 at 2494.843 kb on - strand, within Psyr_2135 at 2495.066 kb on + strand at 2495.066 kb on + strand at 2495.067 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 125 ug/ml remove 2,492,166 - +0.1 2,492,166 - +0.2 2,492,166 - -0.3 2,492,166 - -0.6 2,492,166 - +0.3 2,492,327 + Psyr_2132 0.19 -0.1 2,492,328 - Psyr_2132 0.19 -0.4 2,492,415 + Psyr_2132 0.29 -0.6 2,492,620 + Psyr_2132 0.53 +0.8 2,492,621 - Psyr_2132 0.53 -1.0 2,492,621 - Psyr_2132 0.53 -0.6 2,492,621 - Psyr_2132 0.53 +0.4 2,492,725 - Psyr_2132 0.66 -0.3 2,493,024 + +0.1 2,493,024 + -1.4 2,493,024 + +2.2 2,493,024 + -0.5 2,493,025 - +1.1 2,493,025 - +0.7 2,493,025 - +1.6 2,493,025 - +1.3 2,493,158 + +1.3 2,493,185 - -0.6 2,493,185 - +0.5 2,493,247 - +0.3 2,493,247 - -0.7 2,493,253 + Psyr_2133 0.11 -1.0 2,493,253 + Psyr_2133 0.11 -1.3 2,493,253 + Psyr_2133 0.11 +0.6 2,493,254 - Psyr_2133 0.11 -0.5 2,493,254 - Psyr_2133 0.11 +0.4 2,493,254 - Psyr_2133 0.11 +0.6 2,493,254 - Psyr_2133 0.11 +1.9 2,493,421 + Psyr_2133 0.28 +0.8 2,493,541 + Psyr_2133 0.41 +0.2 2,493,541 + Psyr_2133 0.41 +0.6 2,493,542 - Psyr_2133 0.41 +0.6 2,493,594 + Psyr_2133 0.47 +0.3 2,493,595 - Psyr_2133 0.47 +0.0 2,493,595 - Psyr_2133 0.47 -1.5 2,493,595 - Psyr_2133 0.47 +0.1 2,493,674 - Psyr_2133 0.55 +1.3 2,493,917 + Psyr_2133 0.81 +0.6 2,493,918 - Psyr_2133 0.81 +0.1 2,493,918 - Psyr_2133 0.81 +1.3 2,493,918 - Psyr_2133 0.81 +1.4 2,493,918 - Psyr_2133 0.81 -1.0 2,494,097 - -0.4 2,494,190 + +0.4 2,494,190 + +0.8 2,494,191 - -1.0 2,494,194 + +0.8 2,494,194 + +0.3 2,494,195 - +1.0 2,494,195 - -0.8 2,494,268 - Psyr_2134 0.42 -1.4 2,494,268 - Psyr_2134 0.42 -0.0 2,494,398 + +2.6 2,494,466 - +0.0 2,494,467 + +1.0 2,494,467 + +1.0 2,494,676 + Psyr_2135 0.30 +0.1 2,494,677 - Psyr_2135 0.30 +0.1 2,494,680 + Psyr_2135 0.31 +1.5 2,494,785 + Psyr_2135 0.48 -1.1 2,494,785 + Psyr_2135 0.48 -2.4 2,494,842 + Psyr_2135 0.57 -0.8 2,494,842 + Psyr_2135 0.57 +0.1 2,494,843 - Psyr_2135 0.58 -0.9 2,494,843 - Psyr_2135 0.58 -0.9 2,495,066 + +0.8 2,495,066 + -1.4 2,495,067 - -0.3
Or see this region's nucleotide sequence