Experiment: KB with Nitrofurantoin 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2086 and Psyr_2087 are separated by 68 nucleotides Psyr_2087 and Psyr_2088 are separated by 136 nucleotides
Psyr_2086: Psyr_2086 - 2-methylcitrate synthase, at 2,431,446 to 2,432,573
_2086
Psyr_2087: Psyr_2087 - aconitase / 2-methylcitrate dehydratase (trans-methylaconitate-forming), at 2,432,642 to 2,435,230
_2087
Psyr_2088: Psyr_2088 - 2-methylaconitate cis-trans isomerase, at 2,435,367 to 2,436,557
_2088
Position (kb)
2432
2433
2434
2435
2436 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2431.650 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.650 kb on + strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.651 kb on - strand, within Psyr_2086 at 2431.653 kb on - strand, within Psyr_2086 at 2432.065 kb on - strand, within Psyr_2086 at 2432.236 kb on - strand, within Psyr_2086 at 2432.385 kb on + strand, within Psyr_2086 at 2432.385 kb on + strand, within Psyr_2086 at 2432.386 kb on - strand, within Psyr_2086 at 2432.526 kb on + strand at 2432.526 kb on + strand at 2432.526 kb on + strand at 2432.527 kb on - strand at 2432.571 kb on + strand at 2432.688 kb on - strand at 2432.732 kb on + strand at 2432.747 kb on + strand at 2432.747 kb on + strand at 2432.747 kb on + strand at 2433.632 kb on + strand at 2433.633 kb on - strand, within Psyr_2087 at 2433.633 kb on - strand, within Psyr_2087 at 2433.681 kb on - strand, within Psyr_2087 at 2433.718 kb on - strand, within Psyr_2087 at 2434.172 kb on - strand, within Psyr_2087 at 2434.226 kb on + strand, within Psyr_2087 at 2434.227 kb on - strand, within Psyr_2087 at 2434.227 kb on - strand, within Psyr_2087 at 2434.382 kb on + strand, within Psyr_2087 at 2434.382 kb on + strand, within Psyr_2087 at 2434.382 kb on + strand, within Psyr_2087 at 2434.383 kb on - strand, within Psyr_2087 at 2434.383 kb on - strand, within Psyr_2087 at 2434.383 kb on - strand, within Psyr_2087 at 2434.383 kb on - strand, within Psyr_2087 at 2434.383 kb on - strand, within Psyr_2087 at 2434.383 kb on - strand, within Psyr_2087 at 2434.383 kb on - strand, within Psyr_2087 at 2434.383 kb on - strand, within Psyr_2087 at 2434.383 kb on - strand, within Psyr_2087 at 2434.383 kb on - strand, within Psyr_2087 at 2434.383 kb on - strand, within Psyr_2087 at 2434.460 kb on + strand, within Psyr_2087 at 2434.460 kb on + strand, within Psyr_2087 at 2434.461 kb on - strand, within Psyr_2087 at 2434.461 kb on - strand, within Psyr_2087 at 2434.817 kb on + strand, within Psyr_2087 at 2434.818 kb on - strand, within Psyr_2087 at 2434.818 kb on - strand, within Psyr_2087 at 2434.818 kb on - strand, within Psyr_2087 at 2434.818 kb on - strand, within Psyr_2087 at 2434.818 kb on - strand, within Psyr_2087 at 2435.164 kb on + strand at 2435.164 kb on + strand at 2435.164 kb on + strand at 2435.164 kb on + strand at 2435.165 kb on - strand at 2435.168 kb on + strand at 2435.168 kb on + strand at 2435.228 kb on + strand at 2435.228 kb on + strand at 2435.228 kb on + strand at 2435.228 kb on + strand at 2435.228 kb on + strand at 2435.229 kb on - strand at 2435.229 kb on - strand at 2435.229 kb on - strand at 2435.229 kb on - strand at 2435.232 kb on + strand at 2435.232 kb on + strand at 2435.233 kb on - strand at 2435.274 kb on - strand at 2435.403 kb on + strand at 2435.403 kb on + strand at 2435.403 kb on + strand at 2435.403 kb on + strand at 2435.403 kb on + strand at 2435.403 kb on + strand at 2435.403 kb on + strand at 2435.404 kb on - strand at 2435.404 kb on - strand at 2435.404 kb on - strand at 2435.404 kb on - strand at 2435.404 kb on - strand at 2435.404 kb on - strand at 2435.404 kb on - strand at 2435.404 kb on - strand at 2435.404 kb on - strand at 2435.404 kb on - strand at 2435.622 kb on + strand, within Psyr_2088 at 2435.623 kb on - strand, within Psyr_2088 at 2436.228 kb on + strand, within Psyr_2088
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 125 ug/ml remove 2,431,650 + Psyr_2086 0.18 +0.2 2,431,650 + Psyr_2086 0.18 -0.1 2,431,650 + Psyr_2086 0.18 +1.0 2,431,650 + Psyr_2086 0.18 -0.4 2,431,650 + Psyr_2086 0.18 +0.7 2,431,650 + Psyr_2086 0.18 -0.7 2,431,651 - Psyr_2086 0.18 -0.2 2,431,651 - Psyr_2086 0.18 +0.4 2,431,651 - Psyr_2086 0.18 -0.6 2,431,651 - Psyr_2086 0.18 +0.6 2,431,651 - Psyr_2086 0.18 +0.3 2,431,651 - Psyr_2086 0.18 -0.6 2,431,653 - Psyr_2086 0.18 +0.4 2,432,065 - Psyr_2086 0.55 -0.7 2,432,236 - Psyr_2086 0.70 -0.4 2,432,385 + Psyr_2086 0.83 -0.1 2,432,385 + Psyr_2086 0.83 -0.0 2,432,386 - Psyr_2086 0.83 -1.7 2,432,526 + +0.6 2,432,526 + +0.5 2,432,526 + -1.4 2,432,527 - +0.9 2,432,571 + -1.1 2,432,688 - +0.6 2,432,732 + -1.6 2,432,747 + +1.2 2,432,747 + -0.7 2,432,747 + -0.3 2,433,632 + +0.0 2,433,633 - Psyr_2087 0.38 -1.3 2,433,633 - Psyr_2087 0.38 +0.6 2,433,681 - Psyr_2087 0.40 -1.6 2,433,718 - Psyr_2087 0.42 -0.7 2,434,172 - Psyr_2087 0.59 -0.1 2,434,226 + Psyr_2087 0.61 +0.0 2,434,227 - Psyr_2087 0.61 +0.0 2,434,227 - Psyr_2087 0.61 -1.0 2,434,382 + Psyr_2087 0.67 -1.5 2,434,382 + Psyr_2087 0.67 +0.4 2,434,382 + Psyr_2087 0.67 +0.8 2,434,383 - Psyr_2087 0.67 -0.4 2,434,383 - Psyr_2087 0.67 -0.9 2,434,383 - Psyr_2087 0.67 -0.9 2,434,383 - Psyr_2087 0.67 -0.4 2,434,383 - Psyr_2087 0.67 -2.9 2,434,383 - Psyr_2087 0.67 -0.6 2,434,383 - Psyr_2087 0.67 +0.0 2,434,383 - Psyr_2087 0.67 -0.4 2,434,383 - Psyr_2087 0.67 -3.4 2,434,383 - Psyr_2087 0.67 -2.0 2,434,383 - Psyr_2087 0.67 -0.6 2,434,460 + Psyr_2087 0.70 -0.1 2,434,460 + Psyr_2087 0.70 -0.1 2,434,461 - Psyr_2087 0.70 -0.1 2,434,461 - Psyr_2087 0.70 -3.4 2,434,817 + Psyr_2087 0.84 -0.3 2,434,818 - Psyr_2087 0.84 +0.4 2,434,818 - Psyr_2087 0.84 -1.5 2,434,818 - Psyr_2087 0.84 -2.0 2,434,818 - Psyr_2087 0.84 +0.2 2,434,818 - Psyr_2087 0.84 +0.6 2,435,164 + -0.4 2,435,164 + +0.0 2,435,164 + +2.4 2,435,164 + +0.2 2,435,165 - -1.7 2,435,168 + +0.9 2,435,168 + +1.0 2,435,228 + +0.1 2,435,228 + +0.4 2,435,228 + +0.2 2,435,228 + -0.4 2,435,228 + +0.0 2,435,229 - -1.0 2,435,229 - -1.0 2,435,229 - -2.0 2,435,229 - +1.6 2,435,232 + +0.3 2,435,232 + +0.6 2,435,233 - -1.1 2,435,274 - -0.6 2,435,403 + -0.9 2,435,403 + -0.2 2,435,403 + +1.9 2,435,403 + -0.5 2,435,403 + +0.0 2,435,403 + +0.2 2,435,403 + -1.7 2,435,404 - -0.4 2,435,404 - -1.0 2,435,404 - -0.6 2,435,404 - -2.0 2,435,404 - +0.6 2,435,404 - -1.0 2,435,404 - -0.6 2,435,404 - -0.7 2,435,404 - +0.9 2,435,404 - +0.6 2,435,622 + Psyr_2088 0.21 -0.4 2,435,623 - Psyr_2088 0.21 +0.6 2,436,228 + Psyr_2088 0.72 -0.4
Or see this region's nucleotide sequence