Experiment: KB with Nitrofurantoin 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1986 and Psyr_1987 are separated by 180 nucleotides Psyr_1987 and Psyr_1988 are separated by 1 nucleotides Psyr_1988 and Psyr_1989 are separated by 12 nucleotides
Psyr_1986: Psyr_1986 - aspartate semialdehyde dehydrogenase, at 2,306,109 to 2,307,221
_1986
Psyr_1987: Psyr_1987 - 2-keto-3-deoxygalactonate kinase, at 2,307,402 to 2,308,406
_1987
Psyr_1988: Psyr_1988 - 2-keto-3-deoxy-phosphogalactonate aldolase, at 2,308,408 to 2,309,043
_1988
Psyr_1989: Psyr_1989 - galactonate dehydratase, at 2,309,056 to 2,310,261
_1989
Position (kb)
2307
2308
2309 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2307.281 kb on + strand at 2307.357 kb on + strand at 2307.357 kb on + strand at 2307.360 kb on - strand at 2307.360 kb on - strand at 2307.360 kb on - strand at 2307.360 kb on - strand at 2307.910 kb on + strand, within Psyr_1987 at 2307.910 kb on + strand, within Psyr_1987 at 2308.152 kb on + strand, within Psyr_1987 at 2308.269 kb on + strand, within Psyr_1987 at 2308.269 kb on + strand, within Psyr_1987 at 2308.882 kb on - strand, within Psyr_1988 at 2308.905 kb on - strand, within Psyr_1988 at 2308.905 kb on - strand, within Psyr_1988 at 2308.905 kb on - strand, within Psyr_1988 at 2308.905 kb on - strand, within Psyr_1988 at 2308.999 kb on + strand at 2308.999 kb on + strand at 2309.000 kb on - strand at 2309.000 kb on - strand at 2309.000 kb on - strand at 2309.000 kb on - strand at 2309.000 kb on - strand at 2309.000 kb on - strand at 2309.000 kb on - strand at 2309.085 kb on - strand at 2309.263 kb on + strand, within Psyr_1989 at 2309.263 kb on + strand, within Psyr_1989
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 125 ug/ml remove 2,307,281 + +0.8 2,307,357 + +0.6 2,307,357 + +0.4 2,307,360 - -2.4 2,307,360 - -1.0 2,307,360 - -0.8 2,307,360 - +0.7 2,307,910 + Psyr_1987 0.51 +1.0 2,307,910 + Psyr_1987 0.51 +0.8 2,308,152 + Psyr_1987 0.75 +1.6 2,308,269 + Psyr_1987 0.86 +0.1 2,308,269 + Psyr_1987 0.86 +0.4 2,308,882 - Psyr_1988 0.75 +1.0 2,308,905 - Psyr_1988 0.78 +0.6 2,308,905 - Psyr_1988 0.78 -0.8 2,308,905 - Psyr_1988 0.78 -0.6 2,308,905 - Psyr_1988 0.78 +0.0 2,308,999 + -0.1 2,308,999 + -0.5 2,309,000 - -0.5 2,309,000 - -1.2 2,309,000 - -0.1 2,309,000 - -0.6 2,309,000 - -0.8 2,309,000 - -0.4 2,309,000 - -1.0 2,309,085 - -0.5 2,309,263 + Psyr_1989 0.17 -0.7 2,309,263 + Psyr_1989 0.17 +2.6
Or see this region's nucleotide sequence