Experiment: KB with Nitrofurantoin 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1356 and Psyr_1357 are separated by 8 nucleotides Psyr_1357 and Psyr_1358 are separated by 68 nucleotides Psyr_1358 and Psyr_1359 are separated by 122 nucleotides
Psyr_1356: Psyr_1356 - lipid-A-disaccharide synthase, at 1,536,144 to 1,537,286
_1356
Psyr_1357: Psyr_1357 - RNase HII, at 1,537,295 to 1,537,951
_1357
Psyr_1358: Psyr_1358 - DNA polymerase III, alpha subunit, at 1,538,020 to 1,541,541
_1358
Psyr_1359: Psyr_1359 - acetyl-CoA carboxylase carboxyltransferase subunit alpha, at 1,541,664 to 1,542,617
_1359
Position (kb) 1538
1539
1540
1541
1542 Strain fitness (log2 ratio) -2
-1
0
1 at 1537.318 kb on + strand at 1537.476 kb on - strand, within Psyr_1357 at 1537.476 kb on - strand, within Psyr_1357 at 1537.476 kb on - strand, within Psyr_1357 at 1537.550 kb on - strand, within Psyr_1357 at 1537.601 kb on - strand, within Psyr_1357 at 1537.601 kb on - strand, within Psyr_1357 at 1537.690 kb on - strand, within Psyr_1357 at 1537.764 kb on - strand, within Psyr_1357 at 1537.971 kb on + strand at 1537.971 kb on + strand at 1537.971 kb on + strand at 1537.971 kb on + strand at 1537.971 kb on + strand at 1537.995 kb on + strand at 1538.008 kb on + strand at 1538.008 kb on + strand at 1538.010 kb on + strand at 1538.010 kb on + strand at 1538.010 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 125 ug/ml remove 1,537,318 + -0.8 1,537,476 - Psyr_1357 0.28 +1.4 1,537,476 - Psyr_1357 0.28 -0.6 1,537,476 - Psyr_1357 0.28 +0.5 1,537,550 - Psyr_1357 0.39 -1.4 1,537,601 - Psyr_1357 0.47 -0.6 1,537,601 - Psyr_1357 0.47 +0.2 1,537,690 - Psyr_1357 0.60 -0.1 1,537,764 - Psyr_1357 0.71 -1.6 1,537,971 + -0.1 1,537,971 + -0.2 1,537,971 + +0.0 1,537,971 + -0.1 1,537,971 + -0.0 1,537,995 + -2.2 1,538,008 + -0.0 1,538,008 + -1.0 1,538,010 + +0.0 1,538,010 + -1.8 1,538,010 + -0.7
Or see this region's nucleotide sequence