Experiment: KB with Nitrofurantoin 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1332 and Psyr_1333 are separated by 301 nucleotides Psyr_1333 and Psyr_1334 overlap by 10 nucleotides Psyr_1334 and Psyr_1335 overlap by 4 nucleotides
Psyr_1332: Psyr_1332 - Glycosyl transferase, family 2, at 1,509,862 to 1,512,357
_1332
Psyr_1333: Psyr_1333 - UBA/THIF-type NAD/FAD binding fold protein, at 1,512,659 to 1,513,489
_1333
Psyr_1334: Psyr_1334 - Cysteine desulfuration protein SufE, at 1,513,480 to 1,513,887
_1334
Psyr_1335: Psyr_1335 - Aminotransferase, class V, at 1,513,884 to 1,515,125
_1335
Position (kb)
1512
1513
1514 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1511.710 kb on + strand, within Psyr_1332 at 1511.710 kb on + strand, within Psyr_1332 at 1511.711 kb on - strand, within Psyr_1332 at 1511.711 kb on - strand, within Psyr_1332 at 1511.711 kb on - strand, within Psyr_1332 at 1511.812 kb on - strand, within Psyr_1332 at 1511.924 kb on + strand, within Psyr_1332 at 1511.924 kb on + strand, within Psyr_1332 at 1512.032 kb on - strand, within Psyr_1332 at 1512.032 kb on - strand, within Psyr_1332 at 1512.065 kb on - strand, within Psyr_1332 at 1512.144 kb on - strand at 1512.151 kb on + strand at 1512.151 kb on + strand at 1512.151 kb on + strand at 1512.152 kb on - strand at 1512.152 kb on - strand at 1512.152 kb on - strand at 1512.152 kb on - strand at 1512.154 kb on - strand at 1512.154 kb on - strand at 1512.154 kb on - strand at 1512.176 kb on - strand at 1512.193 kb on + strand at 1512.194 kb on - strand at 1512.224 kb on + strand at 1512.336 kb on + strand at 1512.454 kb on + strand at 1512.454 kb on + strand at 1512.455 kb on - strand at 1512.521 kb on + strand at 1512.537 kb on + strand at 1512.627 kb on + strand at 1512.629 kb on + strand at 1512.652 kb on - strand at 1512.699 kb on + strand at 1512.699 kb on + strand at 1512.699 kb on + strand at 1512.699 kb on + strand at 1512.700 kb on - strand at 1512.700 kb on - strand at 1512.700 kb on - strand at 1512.700 kb on - strand at 1512.700 kb on - strand at 1512.700 kb on - strand at 1512.700 kb on - strand at 1512.700 kb on - strand at 1512.700 kb on - strand at 1512.700 kb on - strand at 1512.701 kb on + strand at 1512.702 kb on - strand at 1512.848 kb on - strand, within Psyr_1333 at 1512.880 kb on + strand, within Psyr_1333 at 1512.881 kb on - strand, within Psyr_1333 at 1513.238 kb on + strand, within Psyr_1333 at 1513.238 kb on + strand, within Psyr_1333 at 1513.239 kb on - strand, within Psyr_1333 at 1513.297 kb on + strand, within Psyr_1333 at 1513.297 kb on + strand, within Psyr_1333 at 1513.298 kb on - strand, within Psyr_1333 at 1513.298 kb on - strand, within Psyr_1333 at 1513.298 kb on - strand, within Psyr_1333 at 1513.298 kb on - strand, within Psyr_1333 at 1513.333 kb on - strand, within Psyr_1333 at 1513.470 kb on + strand at 1513.646 kb on - strand, within Psyr_1334 at 1513.744 kb on + strand, within Psyr_1334 at 1513.744 kb on + strand, within Psyr_1334 at 1513.744 kb on + strand, within Psyr_1334 at 1513.744 kb on + strand, within Psyr_1334 at 1513.744 kb on + strand, within Psyr_1334 at 1513.745 kb on - strand, within Psyr_1334 at 1513.951 kb on + strand at 1513.951 kb on + strand at 1513.952 kb on - strand at 1513.952 kb on - strand at 1514.225 kb on - strand, within Psyr_1335 at 1514.317 kb on + strand, within Psyr_1335 at 1514.417 kb on - strand, within Psyr_1335 at 1514.440 kb on + strand, within Psyr_1335 at 1514.441 kb on - strand, within Psyr_1335
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 125 ug/ml remove 1,511,710 + Psyr_1332 0.74 -0.7 1,511,710 + Psyr_1332 0.74 -0.8 1,511,711 - Psyr_1332 0.74 -0.1 1,511,711 - Psyr_1332 0.74 +1.6 1,511,711 - Psyr_1332 0.74 +0.3 1,511,812 - Psyr_1332 0.78 +0.0 1,511,924 + Psyr_1332 0.83 -0.1 1,511,924 + Psyr_1332 0.83 -1.2 1,512,032 - Psyr_1332 0.87 -0.7 1,512,032 - Psyr_1332 0.87 +0.3 1,512,065 - Psyr_1332 0.88 +0.3 1,512,144 - -1.1 1,512,151 + -0.0 1,512,151 + +0.8 1,512,151 + -0.6 1,512,152 - +0.2 1,512,152 - +1.3 1,512,152 - +0.8 1,512,152 - -1.6 1,512,154 - -0.2 1,512,154 - +0.8 1,512,154 - -0.0 1,512,176 - +0.1 1,512,193 + -0.5 1,512,194 - +0.3 1,512,224 + -0.2 1,512,336 + +0.8 1,512,454 + -0.2 1,512,454 + +0.2 1,512,455 - -0.6 1,512,521 + -1.4 1,512,537 + +2.1 1,512,627 + +0.6 1,512,629 + -0.0 1,512,652 - +0.4 1,512,699 + -0.2 1,512,699 + -1.2 1,512,699 + +1.5 1,512,699 + -1.2 1,512,700 - +0.1 1,512,700 - -1.6 1,512,700 - -0.2 1,512,700 - +0.2 1,512,700 - +0.2 1,512,700 - +1.8 1,512,700 - -0.8 1,512,700 - +0.8 1,512,700 - -0.2 1,512,700 - -0.4 1,512,701 + -0.8 1,512,702 - -0.2 1,512,848 - Psyr_1333 0.23 +0.2 1,512,880 + Psyr_1333 0.27 -0.1 1,512,881 - Psyr_1333 0.27 +0.8 1,513,238 + Psyr_1333 0.70 -0.0 1,513,238 + Psyr_1333 0.70 +0.6 1,513,239 - Psyr_1333 0.70 -1.2 1,513,297 + Psyr_1333 0.77 -0.1 1,513,297 + Psyr_1333 0.77 -1.3 1,513,298 - Psyr_1333 0.77 +0.5 1,513,298 - Psyr_1333 0.77 +0.8 1,513,298 - Psyr_1333 0.77 +1.3 1,513,298 - Psyr_1333 0.77 +1.0 1,513,333 - Psyr_1333 0.81 -0.0 1,513,470 + +1.0 1,513,646 - Psyr_1334 0.41 -0.2 1,513,744 + Psyr_1334 0.65 -0.4 1,513,744 + Psyr_1334 0.65 +0.8 1,513,744 + Psyr_1334 0.65 +1.3 1,513,744 + Psyr_1334 0.65 +0.0 1,513,744 + Psyr_1334 0.65 -0.2 1,513,745 - Psyr_1334 0.65 +2.3 1,513,951 + -0.7 1,513,951 + -0.8 1,513,952 - -0.2 1,513,952 - -0.6 1,514,225 - Psyr_1335 0.27 +2.1 1,514,317 + Psyr_1335 0.35 +0.3 1,514,417 - Psyr_1335 0.43 +0.2 1,514,440 + Psyr_1335 0.45 +0.9 1,514,441 - Psyr_1335 0.45 +2.9
Or see this region's nucleotide sequence