Experiment: KB with Nitrofurantoin 125 ug/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1195 and Psyr_1196 overlap by 1 nucleotides Psyr_1196 and Psyr_1197 are separated by 34 nucleotides Psyr_1197 and Psyr_1198 are separated by 83 nucleotides Psyr_1198 and Psyr_1199 overlap by 23 nucleotides Psyr_1199 and Psyr_1200 are separated by 1 nucleotides
Psyr_1195: Psyr_1195 - type III secretion apparatus lipoprotein, YscJ/HrcJ family, at 1,355,109 to 1,355,915
_1195
Psyr_1196: Psyr_1196 - type III secretion protein HrpD, at 1,355,915 to 1,356,442
_1196
Psyr_1197: Psyr_1197 - type III secretion protein HrpE, at 1,356,477 to 1,357,058
_1197
Psyr_1198: Psyr_1198 - type III secretion protein HrpF, at 1,357,142 to 1,357,366
_1198
Psyr_1199: Psyr_1199 - type III secretion protein HrpG, at 1,357,344 to 1,357,784
_1199
Psyr_1200: Psyr_1200 - outer-membrane type III secretion protein HrcC, at 1,357,786 to 1,359,888
_1200
Position (kb)
1356
1357
1358 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1355.503 kb on + strand, within Psyr_1195 at 1355.503 kb on + strand, within Psyr_1195 at 1355.503 kb on + strand, within Psyr_1195 at 1355.504 kb on - strand, within Psyr_1195 at 1355.936 kb on + strand at 1355.937 kb on - strand at 1355.937 kb on - strand at 1356.320 kb on + strand, within Psyr_1196 at 1356.321 kb on - strand, within Psyr_1196 at 1356.321 kb on - strand, within Psyr_1196 at 1356.420 kb on + strand at 1356.420 kb on + strand at 1356.420 kb on + strand at 1356.420 kb on + strand at 1356.421 kb on - strand at 1356.494 kb on + strand at 1356.494 kb on + strand at 1356.495 kb on - strand at 1356.495 kb on - strand at 1356.495 kb on - strand at 1356.497 kb on + strand at 1356.497 kb on + strand at 1356.546 kb on + strand, within Psyr_1197 at 1356.546 kb on + strand, within Psyr_1197 at 1356.546 kb on + strand, within Psyr_1197 at 1356.547 kb on - strand, within Psyr_1197 at 1356.547 kb on - strand, within Psyr_1197 at 1356.547 kb on - strand, within Psyr_1197 at 1356.547 kb on - strand, within Psyr_1197 at 1356.616 kb on + strand, within Psyr_1197 at 1356.616 kb on + strand, within Psyr_1197 at 1356.616 kb on + strand, within Psyr_1197 at 1356.745 kb on + strand, within Psyr_1197 at 1356.746 kb on - strand, within Psyr_1197 at 1356.746 kb on - strand, within Psyr_1197 at 1356.746 kb on - strand, within Psyr_1197 at 1357.133 kb on + strand at 1357.134 kb on - strand at 1357.146 kb on + strand at 1357.146 kb on + strand at 1357.147 kb on - strand at 1357.147 kb on - strand at 1357.147 kb on - strand at 1357.169 kb on - strand, within Psyr_1198 at 1357.330 kb on + strand, within Psyr_1198 at 1357.331 kb on - strand, within Psyr_1198 at 1357.331 kb on - strand, within Psyr_1198 at 1357.331 kb on - strand, within Psyr_1198 at 1357.523 kb on + strand, within Psyr_1199 at 1357.872 kb on + strand at 1357.879 kb on + strand at 1357.879 kb on + strand at 1357.879 kb on + strand at 1357.880 kb on - strand at 1357.885 kb on + strand at 1357.957 kb on - strand at 1357.992 kb on - strand at 1358.038 kb on + strand, within Psyr_1200 at 1358.038 kb on + strand, within Psyr_1200
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Nitrofurantoin 125 ug/ml remove 1,355,503 + Psyr_1195 0.49 -0.5 1,355,503 + Psyr_1195 0.49 +0.5 1,355,503 + Psyr_1195 0.49 -0.0 1,355,504 - Psyr_1195 0.49 -0.8 1,355,936 + +0.8 1,355,937 - -0.2 1,355,937 - -0.0 1,356,320 + Psyr_1196 0.77 -0.2 1,356,321 - Psyr_1196 0.77 +1.7 1,356,321 - Psyr_1196 0.77 -0.1 1,356,420 + -0.7 1,356,420 + +0.4 1,356,420 + +0.9 1,356,420 + -0.1 1,356,421 - +0.8 1,356,494 + -0.3 1,356,494 + -0.4 1,356,495 - +1.2 1,356,495 - -1.2 1,356,495 - +0.1 1,356,497 + -1.1 1,356,497 + -0.8 1,356,546 + Psyr_1197 0.12 -1.1 1,356,546 + Psyr_1197 0.12 +0.6 1,356,546 + Psyr_1197 0.12 +1.1 1,356,547 - Psyr_1197 0.12 +1.8 1,356,547 - Psyr_1197 0.12 +0.4 1,356,547 - Psyr_1197 0.12 +1.3 1,356,547 - Psyr_1197 0.12 +0.6 1,356,616 + Psyr_1197 0.24 -0.1 1,356,616 + Psyr_1197 0.24 +0.8 1,356,616 + Psyr_1197 0.24 +1.8 1,356,745 + Psyr_1197 0.46 +1.1 1,356,746 - Psyr_1197 0.46 +1.8 1,356,746 - Psyr_1197 0.46 +0.7 1,356,746 - Psyr_1197 0.46 +1.2 1,357,133 + -1.4 1,357,134 - +0.2 1,357,146 + -1.3 1,357,146 + +2.3 1,357,147 - +0.0 1,357,147 - +0.5 1,357,147 - -0.4 1,357,169 - Psyr_1198 0.12 -0.2 1,357,330 + Psyr_1198 0.84 +0.3 1,357,331 - Psyr_1198 0.84 +0.8 1,357,331 - Psyr_1198 0.84 +1.6 1,357,331 - Psyr_1198 0.84 -0.2 1,357,523 + Psyr_1199 0.41 +0.5 1,357,872 + -0.2 1,357,879 + +0.2 1,357,879 + -0.1 1,357,879 + +0.2 1,357,880 - +1.1 1,357,885 + -0.1 1,357,957 - -1.2 1,357,992 - +0.3 1,358,038 + Psyr_1200 0.12 -0.0 1,358,038 + Psyr_1200 0.12 +3.3
Or see this region's nucleotide sequence